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P20290

- BTF3_HUMAN

UniProt

P20290 - BTF3_HUMAN

Protein

Transcription factor BTF3

Gene

BTF3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.1 Publication

    GO - Molecular functioni

    1. poly(A) RNA binding Source: UniProtKB
    2. protein binding Source: IntAct

    GO - Biological processi

    1. in utero embryonic development Source: Ensembl
    2. protein transport Source: UniProtKB-KW
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. transcription from RNA polymerase II promoter Source: ProtInc

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Protein transport, Transcription, Transcription regulation, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor BTF3
    Alternative name(s):
    Nascent polypeptide-associated complex subunit beta
    Short name:
    NAC-beta
    RNA polymerase B transcription factor 3
    Gene namesi
    Name:BTF3
    Synonyms:NACB
    ORF Names:OK/SW-cl.8
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:1125. BTF3.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication
    Note: The heterodimer with NACA is cytoplasmic.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA25445.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 206206Transcription factor BTF3PRO_0000213548Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei30 – 301Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP20290.
    PaxDbiP20290.
    PRIDEiP20290.

    PTM databases

    PhosphoSiteiP20290.

    Expressioni

    Gene expression databases

    ArrayExpressiP20290.
    BgeeiP20290.
    CleanExiHS_BTF3.
    GenevestigatoriP20290.

    Organism-specific databases

    HPAiCAB013007.
    HPA056420.

    Interactioni

    Subunit structurei

    Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit. Both subunits can contact nascent polypeptide chains.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ESR1P033725EBI-1054703,EBI-78473

    Protein-protein interaction databases

    BioGridi107154. 17 interactions.
    IntActiP20290. 6 interactions.
    MINTiMINT-5002480.
    STRINGi9606.ENSP00000369965.

    Structurei

    Secondary structure

    1
    206
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi105 – 1106
    Beta strandi113 – 1208
    Beta strandi122 – 1265
    Turni127 – 1304
    Beta strandi131 – 1366
    Beta strandi138 – 1425
    Helixi143 – 1464
    Helixi149 – 1535
    Helixi158 – 1614

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3LKXX-ray2.50A97-162[»]
    3MCBX-ray1.90B97-154[»]
    ProteinModelPortaliP20290.
    SMRiP20290. Positions 97-154.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP20290.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini82 – 14766NAC-A/BPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi185 – 1884Poly-Asp

    Sequence similaritiesi

    Belongs to the NAC-beta family.Curated
    Contains 1 NAC-A/B (NAC-alpha/beta) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG283773.
    HOGENOMiHOG000261245.
    HOVERGENiHBG004906.
    InParanoidiP20290.
    KOiK01527.
    OMAiDGKAPIA.
    OrthoDBiEOG7CVPXQ.
    PhylomeDBiP20290.
    TreeFamiTF317546.

    Family and domain databases

    InterProiIPR002715. Nas_poly-pep-assoc_cplx_dom.
    [Graphical view]
    PfamiPF01849. NAC. 1 hit.
    [Graphical view]
    PROSITEiPS51151. NAC_AB. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P20290-1) [UniParc]FASTAAdd to Basket

    Also known as: BTF3a

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRRTGAPAQA DSRGRGRARG GCPGGEATLS QPPPRGGTRG QEPQMKETIM    50
    NQEKLAKLQA QVRIGGKGTA RRKKKVVHRT ATADDKKLQF SLKKLGVNNI 100
    SGIEEVNMFT NQGTVIHFNN PKVQASLAAN TFTITGHAET KQLTEMLPSI 150
    LNQLGADSLT SLRRLAEALP KQSVDGKAPL ATGEDDDDEV PDLVENFDEA 200
    SKNEAN 206
    Length:206
    Mass (Da):22,168
    Last modified:February 1, 1991 - v1
    Checksum:i9653AC480EAF64C6
    GO
    Isoform 2 (identifier: P20290-2) [UniParc]FASTAAdd to Basket

    Also known as: BTF3b

    The sequence of this isoform differs from the canonical sequence as follows:
         1-44: Missing.

    Show »
    Length:162
    Mass (Da):17,699
    Checksum:i5062DE8858CEE979
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti41 – 411Q → E in AAA58398. (PubMed:1386332)Curated
    Sequence conflicti68 – 10538Missing in AAA58398. (PubMed:1386332)CuratedAdd
    BLAST
    Sequence conflicti192 – 1965DLVEN → GG(PubMed:1386332)Curated
    Sequence conflicti198 – 1981D → Q(PubMed:1386332)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 4444Missing in isoform 2. 6 PublicationsVSP_013587Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53280 mRNA. Translation: CAA37375.1.
    X53281 mRNA. Translation: CAA37376.1.
    M90357, M90352 Genomic DNA. Translation: AAA58398.1.
    X74070 mRNA. Translation: CAA52200.1.
    AB062126 mRNA. Translation: BAB93458.1.
    BT007120 mRNA. Translation: AAP35784.1.
    AK291125 mRNA. Translation: BAF83814.1.
    CH471084 Genomic DNA. Translation: EAW95727.1.
    BC008062 mRNA. Translation: AAH08062.1.
    CCDSiCCDS34185.1. [P20290-1]
    CCDS4019.1. [P20290-2]
    PIRiJC1235.
    RefSeqiNP_001032726.1. NM_001037637.1. [P20290-1]
    NP_001198.2. NM_001207.4. [P20290-2]
    UniGeneiHs.591768.

    Genome annotation databases

    EnsembliENST00000335895; ENSP00000338516; ENSG00000145741. [P20290-2]
    ENST00000380591; ENSP00000369965; ENSG00000145741. [P20290-1]
    GeneIDi689.
    KEGGihsa:689.
    UCSCiuc003kcq.1. human. [P20290-1]

    Polymorphism databases

    DMDMi115143.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53280 mRNA. Translation: CAA37375.1 .
    X53281 mRNA. Translation: CAA37376.1 .
    M90357 , M90352 Genomic DNA. Translation: AAA58398.1 .
    X74070 mRNA. Translation: CAA52200.1 .
    AB062126 mRNA. Translation: BAB93458.1 .
    BT007120 mRNA. Translation: AAP35784.1 .
    AK291125 mRNA. Translation: BAF83814.1 .
    CH471084 Genomic DNA. Translation: EAW95727.1 .
    BC008062 mRNA. Translation: AAH08062.1 .
    CCDSi CCDS34185.1. [P20290-1 ]
    CCDS4019.1. [P20290-2 ]
    PIRi JC1235.
    RefSeqi NP_001032726.1. NM_001037637.1. [P20290-1 ]
    NP_001198.2. NM_001207.4. [P20290-2 ]
    UniGenei Hs.591768.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3LKX X-ray 2.50 A 97-162 [» ]
    3MCB X-ray 1.90 B 97-154 [» ]
    ProteinModelPortali P20290.
    SMRi P20290. Positions 97-154.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107154. 17 interactions.
    IntActi P20290. 6 interactions.
    MINTi MINT-5002480.
    STRINGi 9606.ENSP00000369965.

    PTM databases

    PhosphoSitei P20290.

    Polymorphism databases

    DMDMi 115143.

    Proteomic databases

    MaxQBi P20290.
    PaxDbi P20290.
    PRIDEi P20290.

    Protocols and materials databases

    DNASUi 689.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000335895 ; ENSP00000338516 ; ENSG00000145741 . [P20290-2 ]
    ENST00000380591 ; ENSP00000369965 ; ENSG00000145741 . [P20290-1 ]
    GeneIDi 689.
    KEGGi hsa:689.
    UCSCi uc003kcq.1. human. [P20290-1 ]

    Organism-specific databases

    CTDi 689.
    GeneCardsi GC05P072794.
    HGNCi HGNC:1125. BTF3.
    HPAi CAB013007.
    HPA056420.
    MIMi 602542. gene.
    neXtProti NX_P20290.
    PharmGKBi PA25445.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG283773.
    HOGENOMi HOG000261245.
    HOVERGENi HBG004906.
    InParanoidi P20290.
    KOi K01527.
    OMAi DGKAPIA.
    OrthoDBi EOG7CVPXQ.
    PhylomeDBi P20290.
    TreeFami TF317546.

    Miscellaneous databases

    ChiTaRSi BTF3. human.
    EvolutionaryTracei P20290.
    GeneWikii BTF3.
    GenomeRNAii 689.
    NextBioi 2836.
    PROi P20290.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P20290.
    Bgeei P20290.
    CleanExi HS_BTF3.
    Genevestigatori P20290.

    Family and domain databases

    InterProi IPR002715. Nas_poly-pep-assoc_cplx_dom.
    [Graphical view ]
    Pfami PF01849. NAC. 1 hit.
    [Graphical view ]
    PROSITEi PS51151. NAC_AB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing and expression of complementary DNA for the general transcription factor BTF3."
      Zheng X.M., Black D., Chambon P., Egly J.-M.
      Nature 344:556-559(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    2. "Genomic structure of the putative BTF3 transcription factor."
      Kanno M., Chalut C., Egly J.-M.
      Gene 117:219-228(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Leukocyte.
    3. "cDNA expression and human 2D-gel data bases: towards integrating protein and DNA information."
      Leffers H., Honore B., Madsen A., Nielsen M.S., Anderson A.H., Celis J.E.
      Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    4. "Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients."
      Shichijo S., Itoh K.
      Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Colon adenocarcinoma.
    5. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    6. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Skin.
    9. "The alpha and beta subunit of the nascent polypeptide-associated complex have distinct functions."
      Beatrix B., Sakai H., Wiedmann M.
      J. Biol. Chem. 275:37838-37845(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH NACA, ASSOCIATION WITH RIBOSOMES, SUBCELLULAR LOCATION.
    10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic kidney.
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Crystal structures of NAC domains of human nascent polypeptide-associated complex (NAC) and its alphaNAC subunit."
      Wang L., Zhang W., Wang L., Zhang X.C., Li X., Rao Z.
      Protein Cell 1:406-416(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 97-154, SUBUNIT.

    Entry informationi

    Entry nameiBTF3_HUMAN
    AccessioniPrimary (citable) accession number: P20290
    Secondary accession number(s): A8K510, Q13893, Q76M56
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3