P20290 (BTF3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor BTF3

Alternative name(s):
RNA polymerase B transcription factor 3

Gene names

Name:	BTF3
Synonyms:	NACB
ORF Names:	OK/SW-cl.8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	General transcription factor. BTF3 can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the NAC-beta family. Contains 1 NAC-A/B (NAC-alpha/beta) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	in utero embryonic development Inferred from electronic annotation. Source: Compara regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoter Traceable author statement Ref.1. Source: ProtInc
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ESR1	P03372	5	EBI-1054703,EBI-78473

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P20290-1)

Also known as: BTF3a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P20290-2)

Also known as: BTF3b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 206

206

Transcription factor BTF3

PRO_0000213548

Regions

Domain

82 – 147

66

NAC-A/B

Compositional bias

185 – 188

4

Poly-Asp

Amino acid modifications

Modified residue

30

1

Phosphoserine Ref.9

Natural variations

Alternative sequence

1 – 44

44

Missing in isoform 2.

VSP_013587

Experimental info

Sequence conflict

41

1

Q → E in AAA58398. Ref.2

Sequence conflict

68 – 105

38

Missing in AAA58398. Ref.2

Sequence conflict

192 – 196

5

DLVEN → GG Ref.2

Sequence conflict

198

1

D → Q Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

206

Helix Strand Turn

Details...

Beta strand

105 – 110

6

Beta strand

113 – 120

8

Beta strand

122 – 126

5

Turn

127 – 130

4

Beta strand

131 – 136

6

Beta strand

138 – 142

5

Helix

143 – 146

4

Helix

149 – 153

5

Helix

158 – 161

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (BTF3a) [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 9653AC480EAF64C6	FASTA	206	22,168
	10 20 30 40 50 60 MRRTGAPAQA DSRGRGRARG GCPGGEATLS QPPPRGGTRG QEPQMKETIM NQEKLAKLQA 70 80 90 100 110 120 QVRIGGKGTA RRKKKVVHRT ATADDKKLQF SLKKLGVNNI SGIEEVNMFT NQGTVIHFNN 130 140 150 160 170 180 PKVQASLAAN TFTITGHAET KQLTEMLPSI LNQLGADSLT SLRRLAEALP KQSVDGKAPL 190 200 ATGEDDDDEV PDLVENFDEA SKNEAN
	Isoform 2 (BTF3b) [UniParc]. Checksum: 5062DE8858CEE979	FASTA	162	17,699
	10 20 30 40 50 60 MKETIMNQEK LAKLQAQVRI GGKGTARRKK KVVHRTATAD DKKLQFSLKK LGVNNISGIE 70 80 90 100 110 120 EVNMFTNQGT VIHFNNPKVQ ASLAANTFTI TGHAETKQLT EMLPSILNQL GADSLTSLRR 130 140 150 160 LAEALPKQSV DGKAPLATGE DDDDEVPDLV ENFDEASKNE AN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and expression of complementary DNA for the general transcription factor BTF3." Zheng X.M., Black D., Chambon P., Egly J.-M. Nature 344:556-559(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[2]	"Genomic structure of the putative BTF3 transcription factor." Kanno M., Chalut C., Egly J.-M. Gene 117:219-228(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Leukocyte.
[3]	"cDNA expression and human 2D-gel data bases: towards integrating protein and DNA information." Leffers H., Honore B., Madsen A., Nielsen M.S., Anderson A.H., Celis J.E. Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]	"Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients." Shichijo S., Itoh K. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Colon adenocarcinoma.
[5]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Skin.
[9]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X53280 mRNA. Translation: CAA37375.1.
X53281 mRNA. Translation: CAA37376.1.
M90357, M90352 Genomic DNA. Translation: AAA58398.1.
X74070 mRNA. Translation: CAA52200.1.
AB062126 mRNA. Translation: BAB93458.1.
BT007120 mRNA. Translation: AAP35784.1.
AK291125 mRNA. Translation: BAF83814.1.
CH471084 Genomic DNA. Translation: EAW95727.1.
BC008062 mRNA. Translation: AAH08062.1.

IPI

IPI00221035.
IPI00419473.

PIR

JC1235.

RefSeq

NP_001032726.1. NM_001037637.1.
NP_001198.2. NM_001207.4.

UniGene

Hs.591768.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3LKX	X-ray	2.50	A	97-162	[»]
3MCB	X-ray	1.90	B	97-154	[»]

ProteinModelPortal

P20290.

ModBase

Search...

Protein-protein interaction databases

IntAct

P20290. 5 interactions.

STRING

9606.ENSP00000369965.

PTM databases

PhosphoSite

P20290.

Polymorphism databases

DMDM

115143.

Proteomic databases

PaxDb

P20290.

PRIDE

P20290.

Protocols and materials databases

DNASU

689.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000335895; ENSP00000338516; ENSG00000145741.
ENST00000380591; ENSP00000369965; ENSG00000145741.

GeneID

689.

KEGG

hsa:689.

UCSC

uc003kcq.1. human.

Organism-specific databases

CTD

689.

GeneCards

GC05P072794.

HGNC

HGNC:1125. BTF3.

HPA

CAB013007.

MIM

602542. gene.

neXtProt

NX_P20290.

PharmGKB

PA25445.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG283773.

HOGENOM

HOG000261245.

HOVERGEN

HBG004906.

InParanoid

P20290.

KO

K01527.

OMA

DGKAPIA.

PhylomeDB

P20290.

Gene expression databases

ArrayExpress

P20290.

Bgee

P20290.

CleanEx

HS_BTF3.

Genevestigator

P20290.

GermOnline

ENSG00000145741. Homo sapiens.

Family and domain databases

InterPro

IPR002715. Nas_poly-pep-assoc_cplx_dom.
[Graphical view]

Pfam

PF01849. NAC. 1 hit.
[Graphical view]

PROSITE

PS51151. NAC_AB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

BTF3. human.

EvolutionaryTrace

P20290.

GenomeRNAi

689.

NextBio

2836.

SOURCE

Search...

Entry information

Entry name

BTF3_HUMAN

Accession

Primary (citable) accession number: P20290
Secondary accession number(s): A8K510, Q13893, Q76M56

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	May 1, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.