SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P20290

- BTF3_HUMAN

UniProt

P20290 - BTF3_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Transcription factor BTF3
Gene
BTF3, NACB, OK/SW-cl.8
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.1 Publication

GO - Molecular functioni

  1. poly(A) RNA binding Source: UniProtKB
  2. protein binding Source: IntAct

GO - Biological processi

  1. in utero embryonic development Source: Ensembl
  2. protein transport Source: UniProtKB-KW
  3. regulation of transcription, DNA-templated Source: UniProtKB-KW
  4. transcription from RNA polymerase II promoter Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Transcription, Transcription regulation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor BTF3
Alternative name(s):
Nascent polypeptide-associated complex subunit beta
Short name:
NAC-beta
RNA polymerase B transcription factor 3
Gene namesi
Name:BTF3
Synonyms:NACB
ORF Names:OK/SW-cl.8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:1125. BTF3.

Subcellular locationi

Cytoplasm. Nucleus
Note: The heterodimer with NACA is cytoplasmic.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25445.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 206206Transcription factor BTF3
PRO_0000213548Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP20290.
PaxDbiP20290.
PRIDEiP20290.

PTM databases

PhosphoSiteiP20290.

Expressioni

Gene expression databases

ArrayExpressiP20290.
BgeeiP20290.
CleanExiHS_BTF3.
GenevestigatoriP20290.

Organism-specific databases

HPAiCAB013007.
HPA056420.

Interactioni

Subunit structurei

Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit. Both subunits can contact nascent polypeptide chains.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ESR1P033725EBI-1054703,EBI-78473

Protein-protein interaction databases

BioGridi107154. 17 interactions.
IntActiP20290. 6 interactions.
MINTiMINT-5002480.
STRINGi9606.ENSP00000369965.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi105 – 1106
Beta strandi113 – 1208
Beta strandi122 – 1265
Turni127 – 1304
Beta strandi131 – 1366
Beta strandi138 – 1425
Helixi143 – 1464
Helixi149 – 1535
Helixi158 – 1614

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LKXX-ray2.50A97-162[»]
3MCBX-ray1.90B97-154[»]
ProteinModelPortaliP20290.
SMRiP20290. Positions 97-154.

Miscellaneous databases

EvolutionaryTraceiP20290.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini82 – 14766NAC-A/B
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi185 – 1884Poly-Asp

Sequence similaritiesi

Belongs to the NAC-beta family.

Phylogenomic databases

eggNOGiNOG283773.
HOGENOMiHOG000261245.
HOVERGENiHBG004906.
InParanoidiP20290.
KOiK01527.
OMAiDGKAPIA.
OrthoDBiEOG7CVPXQ.
PhylomeDBiP20290.
TreeFamiTF317546.

Family and domain databases

InterProiIPR002715. Nas_poly-pep-assoc_cplx_dom.
[Graphical view]
PfamiPF01849. NAC. 1 hit.
[Graphical view]
PROSITEiPS51151. NAC_AB. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P20290-1) [UniParc]FASTAAdd to Basket

Also known as: BTF3a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRRTGAPAQA DSRGRGRARG GCPGGEATLS QPPPRGGTRG QEPQMKETIM    50
NQEKLAKLQA QVRIGGKGTA RRKKKVVHRT ATADDKKLQF SLKKLGVNNI 100
SGIEEVNMFT NQGTVIHFNN PKVQASLAAN TFTITGHAET KQLTEMLPSI 150
LNQLGADSLT SLRRLAEALP KQSVDGKAPL ATGEDDDDEV PDLVENFDEA 200
SKNEAN 206
Length:206
Mass (Da):22,168
Last modified:February 1, 1991 - v1
Checksum:i9653AC480EAF64C6
GO
Isoform 2 (identifier: P20290-2) [UniParc]FASTAAdd to Basket

Also known as: BTF3b

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.

Show »
Length:162
Mass (Da):17,699
Checksum:i5062DE8858CEE979
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4444Missing in isoform 2.
VSP_013587Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti41 – 411Q → E in AAA58398. 1 Publication
Sequence conflicti68 – 10538Missing in AAA58398. 1 Publication
Add
BLAST
Sequence conflicti192 – 1965DLVEN → GG1 Publication
Sequence conflicti198 – 1981D → Q1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53280 mRNA. Translation: CAA37375.1.
X53281 mRNA. Translation: CAA37376.1.
M90357, M90352 Genomic DNA. Translation: AAA58398.1.
X74070 mRNA. Translation: CAA52200.1.
AB062126 mRNA. Translation: BAB93458.1.
BT007120 mRNA. Translation: AAP35784.1.
AK291125 mRNA. Translation: BAF83814.1.
CH471084 Genomic DNA. Translation: EAW95727.1.
BC008062 mRNA. Translation: AAH08062.1.
CCDSiCCDS34185.1. [P20290-1]
CCDS4019.1. [P20290-2]
PIRiJC1235.
RefSeqiNP_001032726.1. NM_001037637.1. [P20290-1]
NP_001198.2. NM_001207.4. [P20290-2]
UniGeneiHs.591768.

Genome annotation databases

EnsembliENST00000335895; ENSP00000338516; ENSG00000145741. [P20290-2]
ENST00000380591; ENSP00000369965; ENSG00000145741. [P20290-1]
GeneIDi689.
KEGGihsa:689.
UCSCiuc003kcq.1. human. [P20290-1]

Polymorphism databases

DMDMi115143.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53280 mRNA. Translation: CAA37375.1 .
X53281 mRNA. Translation: CAA37376.1 .
M90357 , M90352 Genomic DNA. Translation: AAA58398.1 .
X74070 mRNA. Translation: CAA52200.1 .
AB062126 mRNA. Translation: BAB93458.1 .
BT007120 mRNA. Translation: AAP35784.1 .
AK291125 mRNA. Translation: BAF83814.1 .
CH471084 Genomic DNA. Translation: EAW95727.1 .
BC008062 mRNA. Translation: AAH08062.1 .
CCDSi CCDS34185.1. [P20290-1 ]
CCDS4019.1. [P20290-2 ]
PIRi JC1235.
RefSeqi NP_001032726.1. NM_001037637.1. [P20290-1 ]
NP_001198.2. NM_001207.4. [P20290-2 ]
UniGenei Hs.591768.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3LKX X-ray 2.50 A 97-162 [» ]
3MCB X-ray 1.90 B 97-154 [» ]
ProteinModelPortali P20290.
SMRi P20290. Positions 97-154.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 107154. 17 interactions.
IntActi P20290. 6 interactions.
MINTi MINT-5002480.
STRINGi 9606.ENSP00000369965.

PTM databases

PhosphoSitei P20290.

Polymorphism databases

DMDMi 115143.

Proteomic databases

MaxQBi P20290.
PaxDbi P20290.
PRIDEi P20290.

Protocols and materials databases

DNASUi 689.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000335895 ; ENSP00000338516 ; ENSG00000145741 . [P20290-2 ]
ENST00000380591 ; ENSP00000369965 ; ENSG00000145741 . [P20290-1 ]
GeneIDi 689.
KEGGi hsa:689.
UCSCi uc003kcq.1. human. [P20290-1 ]

Organism-specific databases

CTDi 689.
GeneCardsi GC05P072794.
HGNCi HGNC:1125. BTF3.
HPAi CAB013007.
HPA056420.
MIMi 602542. gene.
neXtProti NX_P20290.
PharmGKBi PA25445.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG283773.
HOGENOMi HOG000261245.
HOVERGENi HBG004906.
InParanoidi P20290.
KOi K01527.
OMAi DGKAPIA.
OrthoDBi EOG7CVPXQ.
PhylomeDBi P20290.
TreeFami TF317546.

Miscellaneous databases

ChiTaRSi BTF3. human.
EvolutionaryTracei P20290.
GeneWikii BTF3.
GenomeRNAii 689.
NextBioi 2836.
PROi P20290.
SOURCEi Search...

Gene expression databases

ArrayExpressi P20290.
Bgeei P20290.
CleanExi HS_BTF3.
Genevestigatori P20290.

Family and domain databases

InterProi IPR002715. Nas_poly-pep-assoc_cplx_dom.
[Graphical view ]
Pfami PF01849. NAC. 1 hit.
[Graphical view ]
PROSITEi PS51151. NAC_AB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and expression of complementary DNA for the general transcription factor BTF3."
    Zheng X.M., Black D., Chambon P., Egly J.-M.
    Nature 344:556-559(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
  2. "Genomic structure of the putative BTF3 transcription factor."
    Kanno M., Chalut C., Egly J.-M.
    Gene 117:219-228(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Leukocyte.
  3. "cDNA expression and human 2D-gel data bases: towards integrating protein and DNA information."
    Leffers H., Honore B., Madsen A., Nielsen M.S., Anderson A.H., Celis J.E.
    Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  4. "Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients."
    Shichijo S., Itoh K.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Colon adenocarcinoma.
  5. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  6. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Skin.
  9. "The alpha and beta subunit of the nascent polypeptide-associated complex have distinct functions."
    Beatrix B., Sakai H., Wiedmann M.
    J. Biol. Chem. 275:37838-37845(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NACA, ASSOCIATION WITH RIBOSOMES, SUBCELLULAR LOCATION.
  10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic kidney.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Crystal structures of NAC domains of human nascent polypeptide-associated complex (NAC) and its alphaNAC subunit."
    Wang L., Zhang W., Wang L., Zhang X.C., Li X., Rao Z.
    Protein Cell 1:406-416(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 97-154, SUBUNIT.

Entry informationi

Entry nameiBTF3_HUMAN
AccessioniPrimary (citable) accession number: P20290
Secondary accession number(s): A8K510, Q13893, Q76M56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 9, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi