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Protein

Transcription factor BTF3

Gene

BTF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.1 Publication

GO - Molecular functioni

  • RNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processProtein transport, Transcription, Transcription regulation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor BTF3
Alternative name(s):
Nascent polypeptide-associated complex subunit beta
Short name:
NAC-beta
RNA polymerase B transcription factor 3
Gene namesi
Name:BTF3
Synonyms:NACB
ORF Names:OK/SW-cl.8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000145741.15.
HGNCiHGNC:1125. BTF3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi689.
OpenTargetsiENSG00000145741.
PharmGKBiPA25445.

Polymorphism and mutation databases

BioMutaiBTF3.
DMDMi115143.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002135481 – 206Transcription factor BTF3Add BLAST206

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Omega-N-methylarginineCombined sources1
Modified residuei30PhosphoserineCombined sources1
Modified residuei46N6-methyllysineCombined sources1
Modified residuei54N6-methyllysineCombined sources1
Modified residuei160PhosphothreonineCombined sources1
Modified residuei173PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiP20290.
MaxQBiP20290.
PaxDbiP20290.
PeptideAtlasiP20290.
PRIDEiP20290.
TopDownProteomicsiP20290-1. [P20290-1]
P20290-2. [P20290-2]

PTM databases

iPTMnetiP20290.
PhosphoSitePlusiP20290.
SwissPalmiP20290.

Expressioni

Gene expression databases

BgeeiENSG00000145741.
CleanExiHS_BTF3.
ExpressionAtlasiP20290. baseline and differential.
GenevisibleiP20290. HS.

Organism-specific databases

HPAiCAB013007.
HPA056420.

Interactioni

Subunit structurei

Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit. Both subunits can contact nascent polypeptide chains.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi107154. 58 interactors.
IntActiP20290. 43 interactors.
MINTiMINT-5002480.
STRINGi9606.ENSP00000369965.

Structurei

Secondary structure

1206
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi105 – 110Combined sources6
Beta strandi113 – 120Combined sources8
Beta strandi122 – 126Combined sources5
Turni127 – 130Combined sources4
Beta strandi131 – 136Combined sources6
Beta strandi138 – 142Combined sources5
Helixi143 – 146Combined sources4
Helixi149 – 153Combined sources5
Helixi158 – 161Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LKXX-ray2.50A97-162[»]
3MCBX-ray1.90B97-154[»]
ProteinModelPortaliP20290.
SMRiP20290.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20290.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 147NAC-A/BPROSITE-ProRule annotationAdd BLAST66

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi185 – 188Poly-Asp4

Sequence similaritiesi

Belongs to the NAC-beta family.Curated

Phylogenomic databases

eggNOGiKOG2240. Eukaryota.
ENOG4111JBG. LUCA.
GeneTreeiENSGT00390000006283.
HOGENOMiHOG000261245.
HOVERGENiHBG004906.
InParanoidiP20290.
KOiK01527.
OMAiMKEAIMN.
OrthoDBiEOG091G0TSG.
PhylomeDBiP20290.
TreeFamiTF317546.

Family and domain databases

InterProiView protein in InterPro
IPR002715. Nas_poly-pep-assoc_cplx_dom.
PfamiView protein in Pfam
PF01849. NAC. 1 hit.
SMARTiView protein in SMART
SM01407. NAC. 1 hit.
PROSITEiView protein in PROSITE
PS51151. NAC_AB. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P20290-1) [UniParc]FASTAAdd to basket
Also known as: BTF3a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRTGAPAQA DSRGRGRARG GCPGGEATLS QPPPRGGTRG QEPQMKETIM
60 70 80 90 100
NQEKLAKLQA QVRIGGKGTA RRKKKVVHRT ATADDKKLQF SLKKLGVNNI
110 120 130 140 150
SGIEEVNMFT NQGTVIHFNN PKVQASLAAN TFTITGHAET KQLTEMLPSI
160 170 180 190 200
LNQLGADSLT SLRRLAEALP KQSVDGKAPL ATGEDDDDEV PDLVENFDEA

SKNEAN
Length:206
Mass (Da):22,168
Last modified:February 1, 1991 - v1
Checksum:i9653AC480EAF64C6
GO
Isoform 2 (identifier: P20290-2) [UniParc]FASTAAdd to basket
Also known as: BTF3b

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.

Show »
Length:162
Mass (Da):17,699
Checksum:i5062DE8858CEE979
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41Q → E in AAA58398 (PubMed:1386332).Curated1
Sequence conflicti68 – 105Missing in AAA58398 (PubMed:1386332).CuratedAdd BLAST38
Sequence conflicti192 – 196DLVEN → GG (PubMed:1386332).Curated5
Sequence conflicti198D → Q (PubMed:1386332).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0135871 – 44Missing in isoform 2. 6 PublicationsAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53280 mRNA. Translation: CAA37375.1.
X53281 mRNA. Translation: CAA37376.1.
M90357, M90352 Genomic DNA. Translation: AAA58398.1.
X74070 mRNA. Translation: CAA52200.1.
AB062126 mRNA. Translation: BAB93458.1.
BT007120 mRNA. Translation: AAP35784.1.
AK291125 mRNA. Translation: BAF83814.1.
CH471084 Genomic DNA. Translation: EAW95727.1.
BC008062 mRNA. Translation: AAH08062.1.
CCDSiCCDS34185.1. [P20290-1]
CCDS4019.1. [P20290-2]
PIRiJC1235.
RefSeqiNP_001032726.1. NM_001037637.1. [P20290-1]
NP_001198.2. NM_001207.4. [P20290-2]
UniGeneiHs.591768.

Genome annotation databases

EnsembliENST00000335895; ENSP00000338516; ENSG00000145741. [P20290-2]
ENST00000380591; ENSP00000369965; ENSG00000145741. [P20290-1]
GeneIDi689.
KEGGihsa:689.
UCSCiuc003kcq.2. human. [P20290-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiBTF3_HUMAN
AccessioniPrimary (citable) accession number: P20290
Secondary accession number(s): A8K510, Q13893, Q76M56
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: September 27, 2017
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families