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Protein

Transcription factor BTF3

Gene

BTF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.1 Publication

GO - Molecular functioni

  1. poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  1. in utero embryonic development Source: Ensembl
  2. protein transport Source: UniProtKB-KW
  3. regulation of transcription, DNA-templated Source: UniProtKB-KW
  4. transcription from RNA polymerase II promoter Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Transcription, Transcription regulation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor BTF3
Alternative name(s):
Nascent polypeptide-associated complex subunit beta
Short name:
NAC-beta
RNA polymerase B transcription factor 3
Gene namesi
Name:BTF3
Synonyms:NACB
ORF Names:OK/SW-cl.8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:1125. BTF3.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication
Note: The heterodimer with NACA is cytoplasmic.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25445.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 206206Transcription factor BTF3PRO_0000213548Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP20290.
PaxDbiP20290.
PRIDEiP20290.

PTM databases

PhosphoSiteiP20290.

Expressioni

Gene expression databases

BgeeiP20290.
CleanExiHS_BTF3.
ExpressionAtlasiP20290. baseline and differential.
GenevestigatoriP20290.

Organism-specific databases

HPAiCAB013007.
HPA056420.

Interactioni

Subunit structurei

Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit. Both subunits can contact nascent polypeptide chains.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ESR1P033725EBI-1054703,EBI-78473

Protein-protein interaction databases

BioGridi107154. 19 interactions.
IntActiP20290. 6 interactions.
MINTiMINT-5002480.
STRINGi9606.ENSP00000369965.

Structurei

Secondary structure

1
206
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi105 – 1106Combined sources
Beta strandi113 – 1208Combined sources
Beta strandi122 – 1265Combined sources
Turni127 – 1304Combined sources
Beta strandi131 – 1366Combined sources
Beta strandi138 – 1425Combined sources
Helixi143 – 1464Combined sources
Helixi149 – 1535Combined sources
Helixi158 – 1614Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LKXX-ray2.50A97-162[»]
3MCBX-ray1.90B97-154[»]
ProteinModelPortaliP20290.
SMRiP20290. Positions 97-154.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20290.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini82 – 14766NAC-A/BPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi185 – 1884Poly-Asp

Sequence similaritiesi

Belongs to the NAC-beta family.Curated
Contains 1 NAC-A/B (NAC-alpha/beta) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG283773.
GeneTreeiENSGT00390000006283.
HOGENOMiHOG000261245.
HOVERGENiHBG004906.
InParanoidiP20290.
KOiK01527.
OMAiDGKAPIA.
OrthoDBiEOG7CVPXQ.
PhylomeDBiP20290.
TreeFamiTF317546.

Family and domain databases

InterProiIPR002715. Nas_poly-pep-assoc_cplx_dom.
[Graphical view]
PfamiPF01849. NAC. 1 hit.
[Graphical view]
PROSITEiPS51151. NAC_AB. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P20290-1) [UniParc]FASTAAdd to Basket

Also known as: BTF3a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRTGAPAQA DSRGRGRARG GCPGGEATLS QPPPRGGTRG QEPQMKETIM
60 70 80 90 100
NQEKLAKLQA QVRIGGKGTA RRKKKVVHRT ATADDKKLQF SLKKLGVNNI
110 120 130 140 150
SGIEEVNMFT NQGTVIHFNN PKVQASLAAN TFTITGHAET KQLTEMLPSI
160 170 180 190 200
LNQLGADSLT SLRRLAEALP KQSVDGKAPL ATGEDDDDEV PDLVENFDEA

SKNEAN
Length:206
Mass (Da):22,168
Last modified:February 1, 1991 - v1
Checksum:i9653AC480EAF64C6
GO
Isoform 2 (identifier: P20290-2) [UniParc]FASTAAdd to Basket

Also known as: BTF3b

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.

Show »
Length:162
Mass (Da):17,699
Checksum:i5062DE8858CEE979
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti41 – 411Q → E in AAA58398. (PubMed:1386332)Curated
Sequence conflicti68 – 10538Missing in AAA58398. (PubMed:1386332)CuratedAdd
BLAST
Sequence conflicti192 – 1965DLVEN → GG(PubMed:1386332)Curated
Sequence conflicti198 – 1981D → Q(PubMed:1386332)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4444Missing in isoform 2. 6 PublicationsVSP_013587Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53280 mRNA. Translation: CAA37375.1.
X53281 mRNA. Translation: CAA37376.1.
M90357, M90352 Genomic DNA. Translation: AAA58398.1.
X74070 mRNA. Translation: CAA52200.1.
AB062126 mRNA. Translation: BAB93458.1.
BT007120 mRNA. Translation: AAP35784.1.
AK291125 mRNA. Translation: BAF83814.1.
CH471084 Genomic DNA. Translation: EAW95727.1.
BC008062 mRNA. Translation: AAH08062.1.
CCDSiCCDS34185.1. [P20290-1]
CCDS4019.1. [P20290-2]
PIRiJC1235.
RefSeqiNP_001032726.1. NM_001037637.1. [P20290-1]
NP_001198.2. NM_001207.4. [P20290-2]
UniGeneiHs.591768.

Genome annotation databases

EnsembliENST00000335895; ENSP00000338516; ENSG00000145741. [P20290-2]
ENST00000380591; ENSP00000369965; ENSG00000145741. [P20290-1]
GeneIDi689.
KEGGihsa:689.
UCSCiuc003kcq.1. human. [P20290-1]

Polymorphism databases

DMDMi115143.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53280 mRNA. Translation: CAA37375.1.
X53281 mRNA. Translation: CAA37376.1.
M90357, M90352 Genomic DNA. Translation: AAA58398.1.
X74070 mRNA. Translation: CAA52200.1.
AB062126 mRNA. Translation: BAB93458.1.
BT007120 mRNA. Translation: AAP35784.1.
AK291125 mRNA. Translation: BAF83814.1.
CH471084 Genomic DNA. Translation: EAW95727.1.
BC008062 mRNA. Translation: AAH08062.1.
CCDSiCCDS34185.1. [P20290-1]
CCDS4019.1. [P20290-2]
PIRiJC1235.
RefSeqiNP_001032726.1. NM_001037637.1. [P20290-1]
NP_001198.2. NM_001207.4. [P20290-2]
UniGeneiHs.591768.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LKXX-ray2.50A97-162[»]
3MCBX-ray1.90B97-154[»]
ProteinModelPortaliP20290.
SMRiP20290. Positions 97-154.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107154. 19 interactions.
IntActiP20290. 6 interactions.
MINTiMINT-5002480.
STRINGi9606.ENSP00000369965.

PTM databases

PhosphoSiteiP20290.

Polymorphism databases

DMDMi115143.

Proteomic databases

MaxQBiP20290.
PaxDbiP20290.
PRIDEiP20290.

Protocols and materials databases

DNASUi689.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000335895; ENSP00000338516; ENSG00000145741. [P20290-2]
ENST00000380591; ENSP00000369965; ENSG00000145741. [P20290-1]
GeneIDi689.
KEGGihsa:689.
UCSCiuc003kcq.1. human. [P20290-1]

Organism-specific databases

CTDi689.
GeneCardsiGC05P072794.
HGNCiHGNC:1125. BTF3.
HPAiCAB013007.
HPA056420.
MIMi602542. gene.
neXtProtiNX_P20290.
PharmGKBiPA25445.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG283773.
GeneTreeiENSGT00390000006283.
HOGENOMiHOG000261245.
HOVERGENiHBG004906.
InParanoidiP20290.
KOiK01527.
OMAiDGKAPIA.
OrthoDBiEOG7CVPXQ.
PhylomeDBiP20290.
TreeFamiTF317546.

Miscellaneous databases

ChiTaRSiBTF3. human.
EvolutionaryTraceiP20290.
GeneWikiiBTF3.
GenomeRNAii689.
NextBioi2836.
PROiP20290.
SOURCEiSearch...

Gene expression databases

BgeeiP20290.
CleanExiHS_BTF3.
ExpressionAtlasiP20290. baseline and differential.
GenevestigatoriP20290.

Family and domain databases

InterProiIPR002715. Nas_poly-pep-assoc_cplx_dom.
[Graphical view]
PfamiPF01849. NAC. 1 hit.
[Graphical view]
PROSITEiPS51151. NAC_AB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and expression of complementary DNA for the general transcription factor BTF3."
    Zheng X.M., Black D., Chambon P., Egly J.-M.
    Nature 344:556-559(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
  2. "Genomic structure of the putative BTF3 transcription factor."
    Kanno M., Chalut C., Egly J.-M.
    Gene 117:219-228(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Leukocyte.
  3. "cDNA expression and human 2D-gel data bases: towards integrating protein and DNA information."
    Leffers H., Honore B., Madsen A., Nielsen M.S., Anderson A.H., Celis J.E.
    Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  4. "Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients."
    Shichijo S., Itoh K.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Colon adenocarcinoma.
  5. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  6. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Skin.
  9. "The alpha and beta subunit of the nascent polypeptide-associated complex have distinct functions."
    Beatrix B., Sakai H., Wiedmann M.
    J. Biol. Chem. 275:37838-37845(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NACA, ASSOCIATION WITH RIBOSOMES, SUBCELLULAR LOCATION.
  10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic kidney.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Crystal structures of NAC domains of human nascent polypeptide-associated complex (NAC) and its alphaNAC subunit."
    Wang L., Zhang W., Wang L., Zhang X.C., Li X., Rao Z.
    Protein Cell 1:406-416(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 97-154, SUBUNIT.

Entry informationi

Entry nameiBTF3_HUMAN
AccessioniPrimary (citable) accession number: P20290
Secondary accession number(s): A8K510, Q13893, Q76M56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: February 4, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.