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P20275

- PFKA_SPICI

UniProt

P20275 - PFKA_SPICI

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Protein
ATP-dependent 6-phosphofructokinase
Gene
pfkA
Organism
Spiroplasma citri
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121ATP; via amide nitrogen By similarity
Metal bindingi104 – 1041Magnesium; catalytic By similarity
Active sitei128 – 1281Proton acceptor By similarity
Binding sitei155 – 1551Allosteric activator ADP By similarity
Binding sitei163 – 1631Substrate; shared with dimeric partner By similarity
Binding sitei223 – 2231Substrate By similarity
Binding sitei245 – 2451Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 742ATP By similarity
Nucleotide bindingi103 – 1064ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
OrganismiSpiroplasma citri
Taxonomic identifieri2133 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesEntomoplasmatalesSpiroplasmataceaeSpiroplasma

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327ATP-dependent 6-phosphofructokinaseUniRule annotation
PRO_0000111975Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP20275.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1283Substrate binding By similarity
Regioni170 – 1723Substrate binding By similarity
Regioni186 – 1883Allosteric activator ADP binding By similarity
Regioni214 – 2163Allosteric activator ADP binding By similarity
Regioni251 – 2544Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20275-1 [UniParc]FASTAAdd to Basket

« Hide

MLKKIGILTS GGDSQGMNAA IAGVIKTAHA KGLETYIIRD GYLGLINNWI    50
EVVDNNFADS IMLLGGTVIG SARLPEFKDP EVQKKAVDIL KKQEIAALVV 100
IGGDGSYQGA QRLTELGINC IALPGTIDND ITSSDYTIGF DTAINIVVEA 150
IDRLRDTMQS HNRCSIVEVM GHACGDIALY AGIAGGADII SINEAALSET 200
EIADRVAMLH QAQKRSVIVV VSEMIYPDVH KLAKLVESKS GYITRATVLG 250
HTQRGGNPTA MDRYRAFQMA QFAVEQIIAG VGGLAIGNQG DQIIARPIME 300
ALSIPRSSRK EIWAKFDQLN QNIYQKS 327
Length:327
Mass (Da):35,197
Last modified:February 1, 1996 - v2
Checksum:i19D9D47E176FE2F6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF012877 Genomic DNA. Translation: AAB69998.1.
PIRiE35270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF012877 Genomic DNA. Translation: AAB69998.1 .
PIRi E35270.

3D structure databases

ProteinModelPortali P20275.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Organization and nucleotide sequences of the Spiroplasma citri genes for ribosomal protein S2, elongation factor Ts, spiralin, phosphofructokinase, pyruvate kinase, and an unidentified protein."
    Chevalier C., Saillard C., Bove J.M.
    J. Bacteriol. 172:2693-2703(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 27556 / NCPPB 2647 / R8A2.
  2. "Gene organization and transcriptional analysis of the Spiroplasma citri rpsB/tsf/x operon."
    Le Dantec L., Bove J.M., Saillard C.
    Curr. Microbiol. 37:269-273(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 27556 / NCPPB 2647 / R8A2.

Entry informationi

Entry nameiPFKA_SPICI
AccessioniPrimary (citable) accession number: P20275
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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