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P20275

- PFKA_SPICI

UniProt

P20275 - PFKA_SPICI

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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Spiroplasma citri
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121ATP; via amide nitrogenUniRule annotation
Metal bindingi104 – 1041Magnesium; catalyticUniRule annotation
Active sitei128 – 1281Proton acceptorUniRule annotation
Binding sitei155 – 1551Allosteric activator ADPUniRule annotation
Binding sitei163 – 1631Substrate; shared with dimeric partnerUniRule annotation
Binding sitei223 – 2231SubstrateUniRule annotation
Binding sitei245 – 2451Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 742ATPUniRule annotation
Nucleotide bindingi103 – 1064ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
OrganismiSpiroplasma citri
Taxonomic identifieri2133 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesEntomoplasmatalesSpiroplasmataceaeSpiroplasma

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327ATP-dependent 6-phosphofructokinasePRO_0000111975Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP20275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1283Substrate bindingUniRule annotation
Regioni170 – 1723Substrate bindingUniRule annotation
Regioni186 – 1883Allosteric activator ADP bindingUniRule annotation
Regioni214 – 2163Allosteric activator ADP bindingUniRule annotation
Regioni251 – 2544Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20275-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKKIGILTS GGDSQGMNAA IAGVIKTAHA KGLETYIIRD GYLGLINNWI
60 70 80 90 100
EVVDNNFADS IMLLGGTVIG SARLPEFKDP EVQKKAVDIL KKQEIAALVV
110 120 130 140 150
IGGDGSYQGA QRLTELGINC IALPGTIDND ITSSDYTIGF DTAINIVVEA
160 170 180 190 200
IDRLRDTMQS HNRCSIVEVM GHACGDIALY AGIAGGADII SINEAALSET
210 220 230 240 250
EIADRVAMLH QAQKRSVIVV VSEMIYPDVH KLAKLVESKS GYITRATVLG
260 270 280 290 300
HTQRGGNPTA MDRYRAFQMA QFAVEQIIAG VGGLAIGNQG DQIIARPIME
310 320
ALSIPRSSRK EIWAKFDQLN QNIYQKS
Length:327
Mass (Da):35,197
Last modified:February 1, 1996 - v2
Checksum:i19D9D47E176FE2F6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF012877 Genomic DNA. Translation: AAB69998.1.
PIRiE35270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF012877 Genomic DNA. Translation: AAB69998.1 .
PIRi E35270.

3D structure databases

ProteinModelPortali P20275.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Organization and nucleotide sequences of the Spiroplasma citri genes for ribosomal protein S2, elongation factor Ts, spiralin, phosphofructokinase, pyruvate kinase, and an unidentified protein."
    Chevalier C., Saillard C., Bove J.M.
    J. Bacteriol. 172:2693-2703(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 27556 / NCPPB 2647 / R8A2.
  2. "Gene organization and transcriptional analysis of the Spiroplasma citri rpsB/tsf/x operon."
    Le Dantec L., Bove J.M., Saillard C.
    Curr. Microbiol. 37:269-273(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 27556 / NCPPB 2647 / R8A2.

Entry informationi

Entry nameiPFKA_SPICI
AccessioniPrimary (citable) accession number: P20275
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3