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Protein

Cannabinoid receptor 1

Gene

Cnr1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca2+ channel current.

GO - Molecular functioni

  • cannabinoid receptor activity Source: RGD
  • drug binding Source: RGD

GO - Biological processi

  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: UniProtKB
  • aging Source: RGD
  • axonal fasciculation Source: Ensembl
  • glucose homeostasis Source: Ensembl
  • G-protein coupled receptor signaling pathway Source: RGD
  • learning or memory Source: RGD
  • maternal process involved in female pregnancy Source: RGD
  • memory Source: RGD
  • negative regulation of action potential Source: RGD
  • negative regulation of blood pressure Source: RGD
  • negative regulation of dopamine secretion Source: RGD
  • negative regulation of fatty acid beta-oxidation Source: RGD
  • negative regulation of ion transport Source: RGD
  • negative regulation of mast cell activation Source: RGD
  • negative regulation of nitric-oxide synthase activity Source: RGD
  • positive regulation of acute inflammatory response to antigenic stimulus Source: RGD
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of blood pressure Source: RGD
  • positive regulation of fever generation Source: RGD
  • positive regulation of neuron projection development Source: RGD
  • regulation of feeding behavior Source: RGD
  • regulation of insulin secretion Source: RGD
  • regulation of lipid metabolic process Source: RGD
  • regulation of penile erection Source: RGD
  • regulation of synaptic transmission, GABAergic Source: RGD
  • regulation of synaptic transmission, glutamatergic Source: RGD
  • response to cocaine Source: RGD
  • response to ethanol Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to morphine Source: RGD
  • response to nicotine Source: RGD
  • response to nutrient Source: RGD
  • sensory perception of pain Source: RGD
  • spermatogenesis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-373076. Class A/1 (Rhodopsin-like receptors).
R-RNO-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Cannabinoid receptor 1
Short name:
CB-R
Short name:
CB1
Alternative name(s):
Brain-type cannabinoid receptor
Gene namesi
Name:Cnr1
Synonyms:Skr6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi2369. Cnr1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 117117ExtracellularSequence analysisAdd
BLAST
Transmembranei118 – 14326Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini144 – 15512CytoplasmicSequence analysisAdd
BLAST
Transmembranei156 – 17621Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini177 – 18812ExtracellularSequence analysisAdd
BLAST
Transmembranei189 – 21325Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini214 – 23320CytoplasmicSequence analysisAdd
BLAST
Transmembranei234 – 25623Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini257 – 27418ExtracellularSequence analysisAdd
BLAST
Transmembranei275 – 30026Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini301 – 34545CytoplasmicSequence analysisAdd
BLAST
Transmembranei346 – 36621Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini367 – 37812ExtracellularSequence analysisAdd
BLAST
Transmembranei379 – 40022Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini401 – 47373CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • axon Source: Ensembl
  • growth cone Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • membrane raft Source: CACAO
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2111385.
GuidetoPHARMACOLOGYi56.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Cannabinoid receptor 1PRO_0000069318Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi78 – 781N-linked (GlcNAc...)Sequence analysis
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence analysis
Lipidationi416 – 4161S-palmitoyl cysteine1 Publication
Modified residuei426 – 4261PhosphoserineBy similarity
Modified residuei430 – 4301PhosphoserineBy similarity

Post-translational modificationi

Palmitoylation at Cys-416 is important for recruitment at both plasma membrane and lipid rafts.1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP20272.
PRIDEiP20272.

PTM databases

iPTMnetiP20272.
PhosphoSiteiP20272.
SwissPalmiP20272.

Expressioni

Gene expression databases

GenevisibleiP20272. RN.

Interactioni

Subunit structurei

Interacts (via C-terminus) with CNRIP1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Adora2aP305433EBI-2909800,EBI-2902822

Protein-protein interaction databases

BioGridi247288. 1 interaction.
IntActiP20272. 2 interactions.
MINTiMINT-7266592.
STRINGi10116.ENSRNOP00000010850.

Chemistry

BindingDBiP20272.

Structurei

3D structure databases

ProteinModelPortaliP20272.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000049272.
HOVERGENiHBG051045.
InParanoidiP20272.
KOiK04277.
OMAiVAFCVMW.
OrthoDBiEOG7CK36K.
PhylomeDBiP20272.
TreeFamiTF330052.

Family and domain databases

InterProiIPR000810. Canbinoid_rcpt_1.
IPR002230. Cnbnoid_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PIRSFiPIRSF037995. Cnoid_rcpt_1. 1 hit.
PRINTSiPR00522. CANABINOID1R.
PR00362. CANNABINOIDR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20272-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSILDGLAD TTFRTITTDL LYVGSNDIQY EDIKGDMASK LGYFPQKFPL
60 70 80 90 100
TSFRGSPFQE KMTAGDNSPL VPAGDTTNIT EFYNKSLSSF KENEENIQCG
110 120 130 140 150
ENFMDMECFM ILNPSQQLAI AVLSLTLGTF TVLENLLVLC VILHSRSLRC
160 170 180 190 200
RPSYHFIGSL AVADLLGSVI FVYSFVDFHV FHRKDSPNVF LFKLGGVTAS
210 220 230 240 250
FTASVGSLFL TAIDRYISIH RPLAYKRIVT RPKAVVAFCL MWTIAIVIAV
260 270 280 290 300
LPLLGWNCKK LQSVCSDIFP LIDETYLMFW IGVTSVLLLF IVYAYMYILW
310 320 330 340 350
KAHSHAVRMI QRGTQKSIII HTSEDGKVQV TRPDQARMDI RLAKTLVLIL
360 370 380 390 400
VVLIICWGPL LAIMVYDVFG KMNKLIKTVF AFCSMLCLLN STVNPIIYAL
410 420 430 440 450
RSKDLRHAFR SMFPSCEGTA QPLDNSMGDS DCLHKHANNT ASMHRAAESC
460 470
IKSTVKIAKV TMSVSTDTSA EAL
Length:473
Mass (Da):52,845
Last modified:February 1, 1991 - v1
Checksum:iE59A66AFE17B646C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55812 mRNA. Translation: CAA39332.1.
U40395 mRNA. Translation: AAA99067.1.
PIRiA33117.
RefSeqiNP_036916.1. NM_012784.4.
XP_006238046.1. XM_006237984.2.
UniGeneiRn.89774.

Genome annotation databases

EnsembliENSRNOT00000010850; ENSRNOP00000010850; ENSRNOG00000008223.
GeneIDi25248.
KEGGirno:25248.
UCSCiRGD:2369. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55812 mRNA. Translation: CAA39332.1.
U40395 mRNA. Translation: AAA99067.1.
PIRiA33117.
RefSeqiNP_036916.1. NM_012784.4.
XP_006238046.1. XM_006237984.2.
UniGeneiRn.89774.

3D structure databases

ProteinModelPortaliP20272.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247288. 1 interaction.
IntActiP20272. 2 interactions.
MINTiMINT-7266592.
STRINGi10116.ENSRNOP00000010850.

Chemistry

BindingDBiP20272.
ChEMBLiCHEMBL2111385.
GuidetoPHARMACOLOGYi56.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP20272.
PhosphoSiteiP20272.
SwissPalmiP20272.

Proteomic databases

PaxDbiP20272.
PRIDEiP20272.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010850; ENSRNOP00000010850; ENSRNOG00000008223.
GeneIDi25248.
KEGGirno:25248.
UCSCiRGD:2369. rat.

Organism-specific databases

CTDi1268.
RGDi2369. Cnr1.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000049272.
HOVERGENiHBG051045.
InParanoidiP20272.
KOiK04277.
OMAiVAFCVMW.
OrthoDBiEOG7CK36K.
PhylomeDBiP20272.
TreeFamiTF330052.

Enzyme and pathway databases

ReactomeiR-RNO-373076. Class A/1 (Rhodopsin-like receptors).
R-RNO-418594. G alpha (i) signalling events.

Miscellaneous databases

PROiP20272.

Gene expression databases

GenevisibleiP20272. RN.

Family and domain databases

InterProiIPR000810. Canbinoid_rcpt_1.
IPR002230. Cnbnoid_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PIRSFiPIRSF037995. Cnoid_rcpt_1. 1 hit.
PRINTSiPR00522. CANABINOID1R.
PR00362. CANNABINOIDR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structure of a cannabinoid receptor and functional expression of the cloned cDNA."
    Matsuda L.A., Lolait S.J., Brownstein M.J., Young A.C., Bonner T.I.
    Nature 346:561-564(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain cortex.
  2. "Determination of the cannabinoid receptors in mouse x rat hybridoma NG108-15 cells and rat GH4C1 cells."
    Ho B.Y., Zhao J.
    Neurosci. Lett. 212:123-126(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "An amino-terminal variant of the central cannabinoid receptor resulting from alternative splicing."
    Shire D., Carillon C., Kaghad M., Calandra B., Rinaldi-Carmona M., Le Fur G., Caput D., Ferrara P.
    J. Biol. Chem. 270:3726-3731(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-107.
  4. "CB1 cannabinoid receptor activity is modulated by the cannabinoid receptor interacting protein CRIP 1a."
    Niehaus J.L., Liu Y., Wallis K.T., Egertova M., Bhartur S.G., Mukhopadhyay S., Shi S., He H., Selley D.E., Howlett A.C., Elphick M.R., Lewis D.L.
    Mol. Pharmacol. 72:1557-1566(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CNRIP1.
    Tissue: Brain.
  5. "Effects of palmitoylation of Cys(415) in helix 8 of the CB(1) cannabinoid receptor on membrane localization and signalling."
    Oddi S., Dainese E., Sandiford S., Fezza F., Lanuti M., Chiurchiu V., Totaro A., Catanzaro G., Barcaroli D., De Laurenzi V., Centonze D., Mukhopadhyay S., Selent J., Howlett A.C., Maccarrone M.
    Br. J. Pharmacol. 165:2635-2651(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-416.

Entry informationi

Entry nameiCNR1_RAT
AccessioniPrimary (citable) accession number: P20272
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 8, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.