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P20267

- PO3F1_RAT

UniProt

P20267 - PO3F1_RAT

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Protein

POU domain, class 3, transcription factor 1

Gene

Pou3f1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis. Both in normal development and in response to nerve transection, SCIP expression was transiently activated only during the period of rapid cell division that separates the premyelinating and myelinating phases of Schwann cell differentiation. In cotransfection assays, scip acted as a transcriptional repressor of myelin specific genes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi339 – 39860HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB
  3. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
  4. sequence-specific DNA binding Source: RGD
  5. sequence-specific DNA binding transcription factor activity Source: RGD

GO - Biological processi

  1. glial cell differentiation Source: RGD
  2. negative regulation of transcription, DNA-templated Source: RGD
  3. negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  4. positive regulation of gene expression Source: UniProtKB
  5. positive regulation of transcription, DNA-templated Source: UniProtKB
  6. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  7. regulation of transcription, DNA-templated Source: RGD
  8. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 3, transcription factor 1
Alternative name(s):
Octamer-binding protein 6
Short name:
Oct-6
Octamer-binding transcription factor 6
Short name:
OTF-6
POU domain transcription factor SCIP
Tst-1
Gene namesi
Name:Pou3f1
Synonyms:Oct-6, Otf-6, Otf6, Scip
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi619767. Pou3f1.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 451451POU domain, class 3, transcription factor 1PRO_0000100722Add
BLAST

Expressioni

Tissue specificityi

Neural tissues and testis.

Gene expression databases

GenevestigatoriP20267.

Structurei

3D structure databases

ProteinModelPortaliP20267.
SMRiP20267. Positions 253-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini247 – 32175POU-specificPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

HOVERGENiHBG053120.
InParanoidiP20267.
KOiK09365.
PhylomeDBiP20267.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. Transcription_factor_POU.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20267-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATTAQYLPR GPGGGAGGTG PLMHPDAAAA AAAAAAAERL HAGAAYREVQ
60 70 80 90 100
KLMHHEWLGA GAGHPVGLAH PQWLPTGGGG GGDWAGGPHL EHGKAGGGST
110 120 130 140 150
GRADDGGGGG GFHARLVHQG AAHAGAAWAQ GGTAHHLGPA MSPSPGAGGG
160 170 180 190 200
HQPQPLGLYA QAAYPGGGGG GLAGMLAAGG GGAGPGLHHA LHEDGHEAQL
210 220 230 240 250
EPSPPPHLGA HGHAHGHAHA GGLHAAAAHL HPGAGGGGSS VGEHSDEDAP
260 270 280 290 300
SSDDLEQFAK QFKQRRIKLG FTQADVGLAL GTLYGNVFSQ TTICRFEALQ
310 320 330 340 350
LSFKNMCKLK PLLNKWLEET DSSSGSPTNL DKIAAQGRKR KKRTSIEVGV
360 370 380 390 400
KGALESHFLK CPKPSAHEIT GLADSLQLEK EVVRVWFCNR RQKEKRMTPA
410 420 430 440 450
AGAGHPPMDD VYAPGELGPG GGSASPPSAP PPPPPAALHH HHHHTLPGSV

Q
Length:451
Mass (Da):45,496
Last modified:March 1, 1992 - v2
Checksum:iE32626A759125EAA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti423 – 4231S → G in AAA42118. (PubMed:1705013)Curated
Sequence conflicti423 – 4231S → G in AAA42303. (PubMed:1705013)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M72711 mRNA. Translation: AAA42118.1.
M63712 mRNA. Translation: AAA42303.1.
PIRiA40168.
RefSeqiNP_620193.1. NM_138838.1.
UniGeneiRn.82720.

Genome annotation databases

GeneIDi192110.
KEGGirno:192110.
UCSCiRGD:619767. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M72711 mRNA. Translation: AAA42118.1 .
M63712 mRNA. Translation: AAA42303.1 .
PIRi A40168.
RefSeqi NP_620193.1. NM_138838.1.
UniGenei Rn.82720.

3D structure databases

ProteinModelPortali P20267.
SMRi P20267. Positions 253-399.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 192110.
KEGGi rno:192110.
UCSCi RGD:619767. rat.

Organism-specific databases

CTDi 5453.
RGDi 619767. Pou3f1.

Phylogenomic databases

HOVERGENi HBG053120.
InParanoidi P20267.
KOi K09365.
PhylomeDBi P20267.

Miscellaneous databases

NextBioi 622681.
PROi P20267.

Gene expression databases

Genevestigatori P20267.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. Transcription_factor_POU.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view ]
PIRSFi PIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSi PR00028. POUDOMAIN.
SMARTi SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Expression and activity of the POU transcription factor SCIP."
    Monuki E.S., Kuhn R., Weinmaster G., Trapp B.D., Lemke G.
    Science 249:1300-1303(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Expression of a large family of POU-domain regulatory genes in mammalian brain development."
    He X., Treacy M.N., Simmons D.M., Ingraham H.A., Swanson L.W., Rosenfeld M.G.
    Nature 340:35-42(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 265-369.
  3. Cited for: SEQUENCE REVISION TO 320.
  4. "Tst-1, a member of the POU domain gene family, binds the promoter of the gene encoding the cell surface adhesion molecule P0."
    He X., Gerrero M.R., Simmons D.M., Park R.E., Lin C.R., Swanson L.W., Rosenfeld M.G.
    Mol. Cell. Biol. 11:1739-1744(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 35-451.

Entry informationi

Entry nameiPO3F1_RAT
AccessioniPrimary (citable) accession number: P20267
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: March 1, 1992
Last modified: October 29, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3