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P20267

- PO3F1_RAT

UniProt

P20267 - PO3F1_RAT

Protein

POU domain, class 3, transcription factor 1

Gene

Pou3f1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis. Both in normal development and in response to nerve transection, SCIP expression was transiently activated only during the period of rapid cell division that separates the premyelinating and myelinating phases of Schwann cell differentiation. In cotransfection assays, scip acted as a transcriptional repressor of myelin specific genes.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi339 – 39860HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
    2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB
    3. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
    4. sequence-specific DNA binding Source: RGD
    5. sequence-specific DNA binding transcription factor activity Source: RGD

    GO - Biological processi

    1. glial cell differentiation Source: RGD
    2. negative regulation of transcription, DNA-templated Source: RGD
    3. negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    4. positive regulation of gene expression Source: UniProtKB
    5. positive regulation of transcription, DNA-templated Source: UniProtKB
    6. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    7. regulation of transcription, DNA-templated Source: RGD
    8. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    POU domain, class 3, transcription factor 1
    Alternative name(s):
    Octamer-binding protein 6
    Short name:
    Oct-6
    Octamer-binding transcription factor 6
    Short name:
    OTF-6
    POU domain transcription factor SCIP
    Tst-1
    Gene namesi
    Name:Pou3f1
    Synonyms:Oct-6, Otf-6, Otf6, Scip
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi619767. Pou3f1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 451451POU domain, class 3, transcription factor 1PRO_0000100722Add
    BLAST

    Expressioni

    Tissue specificityi

    Neural tissues and testis.

    Gene expression databases

    GenevestigatoriP20267.

    Structurei

    3D structure databases

    ProteinModelPortaliP20267.
    SMRiP20267. Positions 253-399.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini247 – 32175POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    HOVERGENiHBG053120.
    KOiK09365.
    PhylomeDBiP20267.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    IPR016362. Transcription_factor_POU.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
    PRINTSiPR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20267-1 [UniParc]FASTAAdd to Basket

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    MATTAQYLPR GPGGGAGGTG PLMHPDAAAA AAAAAAAERL HAGAAYREVQ    50
    KLMHHEWLGA GAGHPVGLAH PQWLPTGGGG GGDWAGGPHL EHGKAGGGST 100
    GRADDGGGGG GFHARLVHQG AAHAGAAWAQ GGTAHHLGPA MSPSPGAGGG 150
    HQPQPLGLYA QAAYPGGGGG GLAGMLAAGG GGAGPGLHHA LHEDGHEAQL 200
    EPSPPPHLGA HGHAHGHAHA GGLHAAAAHL HPGAGGGGSS VGEHSDEDAP 250
    SSDDLEQFAK QFKQRRIKLG FTQADVGLAL GTLYGNVFSQ TTICRFEALQ 300
    LSFKNMCKLK PLLNKWLEET DSSSGSPTNL DKIAAQGRKR KKRTSIEVGV 350
    KGALESHFLK CPKPSAHEIT GLADSLQLEK EVVRVWFCNR RQKEKRMTPA 400
    AGAGHPPMDD VYAPGELGPG GGSASPPSAP PPPPPAALHH HHHHTLPGSV 450
    Q 451
    Length:451
    Mass (Da):45,496
    Last modified:March 1, 1992 - v2
    Checksum:iE32626A759125EAA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti423 – 4231S → G in AAA42118. (PubMed:1705013)Curated
    Sequence conflicti423 – 4231S → G in AAA42303. (PubMed:1705013)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M72711 mRNA. Translation: AAA42118.1.
    M63712 mRNA. Translation: AAA42303.1.
    PIRiA40168.
    RefSeqiNP_620193.1. NM_138838.1.
    UniGeneiRn.82720.

    Genome annotation databases

    GeneIDi192110.
    KEGGirno:192110.
    UCSCiRGD:619767. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M72711 mRNA. Translation: AAA42118.1 .
    M63712 mRNA. Translation: AAA42303.1 .
    PIRi A40168.
    RefSeqi NP_620193.1. NM_138838.1.
    UniGenei Rn.82720.

    3D structure databases

    ProteinModelPortali P20267.
    SMRi P20267. Positions 253-399.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 192110.
    KEGGi rno:192110.
    UCSCi RGD:619767. rat.

    Organism-specific databases

    CTDi 5453.
    RGDi 619767. Pou3f1.

    Phylogenomic databases

    HOVERGENi HBG053120.
    KOi K09365.
    PhylomeDBi P20267.

    Miscellaneous databases

    NextBioi 622681.
    PROi P20267.

    Gene expression databases

    Genevestigatori P20267.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    IPR016362. Transcription_factor_POU.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002629. Transcription_factor_POU. 1 hit.
    PRINTSi PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression and activity of the POU transcription factor SCIP."
      Monuki E.S., Kuhn R., Weinmaster G., Trapp B.D., Lemke G.
      Science 249:1300-1303(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Expression of a large family of POU-domain regulatory genes in mammalian brain development."
      He X., Treacy M.N., Simmons D.M., Ingraham H.A., Swanson L.W., Rosenfeld M.G.
      Nature 340:35-42(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 265-369.
    3. Cited for: SEQUENCE REVISION TO 320.
    4. "Tst-1, a member of the POU domain gene family, binds the promoter of the gene encoding the cell surface adhesion molecule P0."
      He X., Gerrero M.R., Simmons D.M., Park R.E., Lin C.R., Swanson L.W., Rosenfeld M.G.
      Mol. Cell. Biol. 11:1739-1744(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 35-451.

    Entry informationi

    Entry nameiPO3F1_RAT
    AccessioniPrimary (citable) accession number: P20267
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3