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Protein

POU domain, class 3, transcription factor 1

Gene

Pou3f1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis. Both in normal development and in response to nerve transection, SCIP expression was transiently activated only during the period of rapid cell division that separates the premyelinating and myelinating phases of Schwann cell differentiation. In cotransfection assays, scip acted as a transcriptional repressor of myelin specific genes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi339 – 39860HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • brain development Source: InterPro
  • glial cell differentiation Source: RGD
  • negative regulation of transcription, DNA-templated Source: RGD
  • negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of transcription, DNA-templated Source: RGD
  • transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 3, transcription factor 1
Alternative name(s):
Octamer-binding protein 6
Short name:
Oct-6
Octamer-binding transcription factor 6
Short name:
OTF-6
POU domain transcription factor SCIP
Tst-1
Gene namesi
Name:Pou3f1
Synonyms:Oct-6, Otf-6, Otf6, Scip
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619767. Pou3f1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 451451POU domain, class 3, transcription factor 1PRO_0000100722Add
BLAST

Expressioni

Tissue specificityi

Neural tissues and testis.

Gene expression databases

GenevisibleiP20267. RN.

Structurei

3D structure databases

ProteinModelPortaliP20267.
SMRiP20267. Positions 253-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini247 – 32175POU-specificPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

HOVERGENiHBG053120.
InParanoidiP20267.
KOiK09365.
PhylomeDBiP20267.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. TF_POU_3.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20267-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTAQYLPR GPGGGAGGTG PLMHPDAAAA AAAAAAAERL HAGAAYREVQ
60 70 80 90 100
KLMHHEWLGA GAGHPVGLAH PQWLPTGGGG GGDWAGGPHL EHGKAGGGST
110 120 130 140 150
GRADDGGGGG GFHARLVHQG AAHAGAAWAQ GGTAHHLGPA MSPSPGAGGG
160 170 180 190 200
HQPQPLGLYA QAAYPGGGGG GLAGMLAAGG GGAGPGLHHA LHEDGHEAQL
210 220 230 240 250
EPSPPPHLGA HGHAHGHAHA GGLHAAAAHL HPGAGGGGSS VGEHSDEDAP
260 270 280 290 300
SSDDLEQFAK QFKQRRIKLG FTQADVGLAL GTLYGNVFSQ TTICRFEALQ
310 320 330 340 350
LSFKNMCKLK PLLNKWLEET DSSSGSPTNL DKIAAQGRKR KKRTSIEVGV
360 370 380 390 400
KGALESHFLK CPKPSAHEIT GLADSLQLEK EVVRVWFCNR RQKEKRMTPA
410 420 430 440 450
AGAGHPPMDD VYAPGELGPG GGSASPPSAP PPPPPAALHH HHHHTLPGSV

Q
Length:451
Mass (Da):45,496
Last modified:March 1, 1992 - v2
Checksum:iE32626A759125EAA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti423 – 4231S → G in AAA42118 (PubMed:1705013).Curated
Sequence conflicti423 – 4231S → G in AAA42303 (PubMed:1705013).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M72711 mRNA. Translation: AAA42118.1.
M63712 mRNA. Translation: AAA42303.1.
PIRiA40168.
RefSeqiNP_620193.1. NM_138838.1.
UniGeneiRn.82720.

Genome annotation databases

GeneIDi192110.
KEGGirno:192110.
UCSCiRGD:619767. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M72711 mRNA. Translation: AAA42118.1.
M63712 mRNA. Translation: AAA42303.1.
PIRiA40168.
RefSeqiNP_620193.1. NM_138838.1.
UniGeneiRn.82720.

3D structure databases

ProteinModelPortaliP20267.
SMRiP20267. Positions 253-399.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi192110.
KEGGirno:192110.
UCSCiRGD:619767. rat.

Organism-specific databases

CTDi5453.
RGDi619767. Pou3f1.

Phylogenomic databases

HOVERGENiHBG053120.
InParanoidiP20267.
KOiK09365.
PhylomeDBiP20267.

Miscellaneous databases

NextBioi622681.
PROiP20267.

Gene expression databases

GenevisibleiP20267. RN.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. TF_POU_3.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression and activity of the POU transcription factor SCIP."
    Monuki E.S., Kuhn R., Weinmaster G., Trapp B.D., Lemke G.
    Science 249:1300-1303(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Expression of a large family of POU-domain regulatory genes in mammalian brain development."
    He X., Treacy M.N., Simmons D.M., Ingraham H.A., Swanson L.W., Rosenfeld M.G.
    Nature 340:35-42(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 265-369.
  3. Cited for: SEQUENCE REVISION TO 320.
  4. "Tst-1, a member of the POU domain gene family, binds the promoter of the gene encoding the cell surface adhesion molecule P0."
    He X., Gerrero M.R., Simmons D.M., Park R.E., Lin C.R., Swanson L.W., Rosenfeld M.G.
    Mol. Cell. Biol. 11:1739-1744(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 35-451.

Entry informationi

Entry nameiPO3F1_RAT
AccessioniPrimary (citable) accession number: P20267
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: March 1, 1992
Last modified: June 24, 2015
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.