Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

POU domain, class 4, transcription factor 1

Gene

Pou4f1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Multifunctional transcription factor with different regions mediating its different effects (PubMed:10640682). Acts by binding (via its C-terminal domain) to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes. Regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages (PubMed:11053412). It has been shown that activation of some of these genes requires its N-terminal domain, maybe through a neuronal-specific cofactor. Ativates BCL2 expression and protects neuronal cells from apoptosis (via the N-terminal domain). Induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins. Exerts its major developmental effects in somatosensory neurons and in brainstem nuclei involved in motor control. Stimulates the binding affinity of the nuclear estrogene receptor ESR1 to DNA estrogen response element (ERE), and hence modulates ESR1-induced transcriptional activity. May positively regulate POU4F2 and POU4F3. Regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord. Plays a role in TNFSF11-mediated terminal osteoclast differentiation. Negatively regulates its own expression interacting directly with a highly conserved autoregulatory domain surrounding the transcription initiation site (By similarity).By similarity2 Publications
Isoform 2: Able to act as transcription factor, cannot regulate the expression of the same subset of genes than isoform 1. Does not have anitapoptotic effect on neuronal cells.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi356 – 415HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 4, transcription factor 1
Alternative name(s):
Brain-specific homeobox/POU domain protein 3A
Short name:
Brain-3A
Short name:
Brn-3A
Gene namesi
Name:Pou4f1
Synonyms:Brn-3, Brn3, Brn3a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620074. Pou4f1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007381 – 420POU domain, class 4, transcription factor 1Add BLAST420

Proteomic databases

PaxDbiP20266.

Expressioni

Tissue specificityi

Detected in brain, spinal cord and dorsal root ganglion (PubMed:2739723, PubMed:8835784). Isoform 2 is detected in brain, spinal cord, dorsal root ganglion and spleen (PubMed:8835784).2 Publications

Interactioni

Subunit structurei

Interacts (via N-terminus) with RIT2; the interaction controls POU4F1 transactivation activity on some neuronal target genes. Isoform 1 interacts with POU4F2; this interaction inhibits both POU4F1 DNA-binding and transcriptional activities. Isoform 1 interacts (C-terminus) with ESR1 (via DNA-binding domain); this interaction decreases the estrogen receptor ESR1 transcriptional activity in a DNA- and ligand 17-beta-estradiol-independent manner.By similarity

Protein-protein interaction databases

IntActiP20266. 1 interactor.
MINTiMINT-1514701.
STRINGi10116.ENSRNOP00000012828.

Structurei

3D structure databases

ProteinModelPortaliP20266.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini261 – 338POU-specificPROSITE-ProRule annotationAdd BLAST78

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 66POU-IV box10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi100 – 108His-richPROSITE-ProRule annotation9
Compositional biasi130 – 185Gly-richPROSITE-ProRule annotationAdd BLAST56
Compositional biasi204 – 257Ala-richPROSITE-ProRule annotationAdd BLAST54

Domaini

The C-terminal domain is able to act as both DNA-binding domain and a transcriptional activator. The N-terminal domain is also required for transactivation activity on some target genes acting as a discrete activation domain. Neurite outgrowth and expression of genes required for synapse formation are primarily dependent on the C-terminal domain, however the N-terminal domain is required for maximal induction.By similarity

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG1168. Eukaryota.
ENOG410XPNX. LUCA.
HOGENOMiHOG000116305.
HOVERGENiHBG053120.
InParanoidiP20266.
KOiK09366.

Family and domain databases

CDDicd00086. homeodomain. 1 hit.
InterProiView protein in InterPro
IPR009057. Homeobox-like.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR015584. POU4-TF1.
IPR000327. POU_dom.
PANTHERiPTHR11636:SF90. PTHR11636:SF90. 1 hit.
PfamiView protein in Pfam
PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiView protein in SMART
SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiView protein in PROSITE
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: P20266-1) [UniParc]FASTAAdd to basket
Also known as: Brn-3A-Long1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMSMNSKQPH FAMHPTLPEH KYPSLHSSSE AIRRACLPTP PLQSNLFASL
60 70 80 90 100
DETLLARAEA LAAVDIAVSQ GKSHPFKPDA TYHTMNSVPC TSTSTVPLAH
110 120 130 140 150
HHHHHHHHQA LEPGDLLDHI SSPSLALMAG AGGAGAAGGG GAPTRPRGAG
160 170 180 190 200
GPGGGGGPGG GGPGVAGRRR GPGGGGGGPG GGLLGXSAHP HPHMHGXGHL
210 220 230 240 250
SHPAAAAAMN MPSGLPHPGL VAAAAHHGAA AAAAAAAAGQ VAAASAAAAV
260 270 280 290 300
VGAAGLASIC DSDTDPRELE AFAERFKQRR IKLGVTQADV GSALANLKIP
310 320 330 340 350
GVGSLSQSTI CRFESLTLSH NNMIALKPIL QAWLEEAEGA QREKMNKPEL
360 370 380 390 400
FNGGEKKRKR TSIAAPEKRS LEAYFAVQPR PSSEKIAAIA EKLDLKKNVV
410 420
RVWFCNQRQK QKRMKFSATY
Length:420
Mass (Da):43,275
Last modified:November 2, 2016 - v3
Checksum:i64B4FAE98FE27011
GO
Isoform 21 Publication (identifier: P20266-2) [UniParc]FASTAAdd to basket
Also known as: Brn-3A-Short1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-84: Missing.

Show »
Length:336
Mass (Da):34,068
Checksum:iC7BF991FAE85F777
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti340A → P (PubMed:2739723).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0586381 – 84Missing in isoform 2. 1 PublicationAdd BLAST84

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF390075 mRNA. Translation: AAK70502.1.
PIRiS05045.
RefSeqiXP_008769153.1. XM_008770931.2. [P20266-1]
UniGeneiRn.232568.

Genome annotation databases

GeneIDi114503.
KEGGirno:114503.
UCSCiRGD:620074. rat. [P20266-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPO4F1_RAT
AccessioniPrimary (citable) accession number: P20266
Secondary accession number(s): Q91XM4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: November 2, 2016
Last modified: September 27, 2017
This is version 141 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families