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P20265

- PO3F2_HUMAN

UniProt

P20265 - PO3F2_HUMAN

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Protein

POU domain, class 3, transcription factor 2

Gene
POU3F2, BRN2, OCT7, OTF7
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi354 – 41360HomeoboxAdd
BLAST

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. protein binding Source: IntAct
  3. RNA polymerase II transcription coactivator activity Source: Ensembl
  4. sequence-specific DNA binding Source: Ensembl
  5. sequence-specific DNA binding transcription factor activity Source: ProtInc

GO - Biological processi

  1. astrocyte development Source: Ensembl
  2. cellular response to organic substance Source: Ensembl
  3. cerebral cortex radially oriented cell migration Source: Ensembl
  4. epidermis development Source: Ensembl
  5. forebrain ventricular zone progenitor cell division Source: Ensembl
  6. hypothalamus cell differentiation Source: Ensembl
  7. myelination in peripheral nervous system Source: Ensembl
  8. neurohypophysis development Source: Ensembl
  9. neuron differentiation Source: Ensembl
  10. positive regulation of cell proliferation Source: MGI
  11. positive regulation of multicellular organism growth Source: Ensembl
  12. regulation of axonogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiP20265.

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 3, transcription factor 2
Alternative name(s):
Brain-specific homeobox/POU domain protein 2
Short name:
Brain-2
Short name:
Brn-2
Nervous system-specific octamer-binding transcription factor N-Oct-3
Octamer-binding protein 7
Short name:
Oct-7
Octamer-binding transcription factor 7
Short name:
OTF-7
Gene namesi
Name:POU3F2
Synonyms:BRN2, OCT7, OTF7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 6

Organism-specific databases

HGNCiHGNC:9215. POU3F2.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
  2. transcription factor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33539.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443POU domain, class 3, transcription factor 2PRO_0000013495Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei341 – 3411Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP20265.
PaxDbiP20265.
PRIDEiP20265.

PTM databases

PhosphoSiteiP20265.

Expressioni

Tissue specificityi

Expressed specifically in the neuroectodermal cell lineage.

Gene expression databases

BgeeiP20265.
CleanExiHS_POU3F2.
GenevestigatoriP20265.

Interactioni

Subunit structurei

Interacts with PQBP1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1167176,EBI-1167176
EP300Q094723EBI-1167176,EBI-447295
PAX3P237602EBI-1167176,EBI-1167564
SOX10P566933EBI-1167176,EBI-1167533
TBPP202262EBI-1167176,EBI-355371

Protein-protein interaction databases

BioGridi111450. 9 interactions.
IntActiP20265. 6 interactions.
MINTiMINT-2801637.
STRINGi9606.ENSP00000329170.

Structurei

3D structure databases

ProteinModelPortaliP20265.
SMRiP20265. Positions 265-414.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini262 – 33675POU-specificAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi68 – 9023Poly-GlyAdd
BLAST
Compositional biasi125 – 14925Poly-GlnAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG261729.
HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiP20265.
KOiK09365.
OMAiMHNSSNN.
OrthoDBiEOG7DJSMG.
PhylomeDBiP20265.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. Transcription_factor_POU.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative initiation. Align

Isoform N-OCT 3 (identifier: P20265-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL VQGDYGALQS    50
NGHPLSHAHQ WITALSHGGG GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ 100
PDIKPSVVVQ QGGRGDELHG PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQR 150
PPHLVHHAAN HHPGPGAWRS AAAAAHLPPS MGASNGGLLY SQPSFTVNGM 200
LGAGGQPAGL HHHGLRDAHD EPHHADHHPH PHSHPHQQPP PPPPPQGPPG 250
HPGAHHDPHS DEDTPTSDDL EQFAKQFKQR RIKLGFTQAD VGLALGTLYG 300
NVFSQTTICR FEALQLSFKN MCKLKPLLNK WLEEADSSSG SPTSIDKIAA 350
QGRKRKKRTS IEVSVKGALE SHFLKCPKPS AQEITSLADS LQLEKEVVRV 400
WFCNRRQKEK RMTPPGGTLP GAEDVYGGSR DTPPHHGVQT PVQ 443
Length:443
Mass (Da):46,893
Last modified:September 13, 2005 - v4
Checksum:iD470360894D788C6
GO
Isoform N-OCT 5A (identifier: P20265-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-180: Missing.

Show »
Length:263
Mass (Da):28,723
Checksum:i8E6F82E65137A377
GO
Isoform N-OCT 5B (identifier: P20265-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-199: Missing.

Show »
Length:244
Mass (Da):26,841
Checksum:iD185AA0BBD6C33C7
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 199199Missing in isoform N-OCT 5B. VSP_018750Add
BLAST
Alternative sequencei1 – 180180Missing in isoform N-OCT 5A. VSP_018749Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261G → A in CAA77990. 1 Publication
Sequence conflicti170 – 1701S → T1 Publication
Sequence conflicti170 – 1701S → T1 Publication
Sequence conflicti207 – 2071P → S in AAH51699. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11933 mRNA. Translation: CAA77990.1.
L37868 Genomic DNA. Translation: AAB59611.1.
AL022395 Genomic DNA. Translation: CAB37982.1.
BC051699 mRNA. Translation: AAH51699.2.
CCDSiCCDS5040.1. [P20265-1]
PIRiS29334.
RefSeqiNP_005595.2. NM_005604.3. [P20265-1]
UniGeneiHs.182505.

Genome annotation databases

EnsembliENST00000328345; ENSP00000329170; ENSG00000184486. [P20265-1]
GeneIDi5454.
KEGGihsa:5454.
UCSCiuc003ppe.3. human. [P20265-1]

Polymorphism databases

DMDMi78100757.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11933 mRNA. Translation: CAA77990.1 .
L37868 Genomic DNA. Translation: AAB59611.1 .
AL022395 Genomic DNA. Translation: CAB37982.1 .
BC051699 mRNA. Translation: AAH51699.2 .
CCDSi CCDS5040.1. [P20265-1 ]
PIRi S29334.
RefSeqi NP_005595.2. NM_005604.3. [P20265-1 ]
UniGenei Hs.182505.

3D structure databases

ProteinModelPortali P20265.
SMRi P20265. Positions 265-414.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111450. 9 interactions.
IntActi P20265. 6 interactions.
MINTi MINT-2801637.
STRINGi 9606.ENSP00000329170.

Chemistry

BindingDBi P20265.
ChEMBLi CHEMBL4189.

PTM databases

PhosphoSitei P20265.

Polymorphism databases

DMDMi 78100757.

Proteomic databases

MaxQBi P20265.
PaxDbi P20265.
PRIDEi P20265.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000328345 ; ENSP00000329170 ; ENSG00000184486 . [P20265-1 ]
GeneIDi 5454.
KEGGi hsa:5454.
UCSCi uc003ppe.3. human. [P20265-1 ]

Organism-specific databases

CTDi 5454.
GeneCardsi GC06P099295.
HGNCi HGNC:9215. POU3F2.
MIMi 600494. gene.
neXtProti NX_P20265.
PharmGKBi PA33539.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG261729.
HOGENOMi HOG000116303.
HOVERGENi HBG053120.
InParanoidi P20265.
KOi K09365.
OMAi MHNSSNN.
OrthoDBi EOG7DJSMG.
PhylomeDBi P20265.
TreeFami TF316413.

Enzyme and pathway databases

SignaLinki P20265.

Miscellaneous databases

GeneWikii POU3F2.
GenomeRNAii 5454.
NextBioi 21109.
PROi P20265.
SOURCEi Search...

Gene expression databases

Bgeei P20265.
CleanExi HS_POU3F2.
Genevestigatori P20265.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. Transcription_factor_POU.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view ]
PIRSFi PIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSi PR00028. POUDOMAIN.
SMARTi SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning of human N-Oct3, a nervous-system specific POU domain transcription factor binding to the octamer DNA motif."
    Schreiber E., Tobler A., Malipiero U., Schaffner W., Fontana A.
    Nucleic Acids Res. 21:253-258(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The brn-2 gene regulates the melanocytic phenotype and tumorigenic potential of human melanoma cells."
    Angus J., Thomson F., Murphy K., Baker E., Sutherland G.R., Parsons P.G., Sturm R.A.
    Oncogene 11:691-700(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  3. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. "Expression of a large family of POU-domain regulatory genes in mammalian brain development."
    He X., Treacy M.N., Simmons D.M., Ingraham H.A., Swanson L.W., Rosenfeld M.G.
    Nature 340:35-42(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 280-404.
    Tissue: Brain.
  6. "PQBP-1, a novel polyglutamine tract binding protein, inhibits transcription activation by Brn-2 and affects cell survival."
    Waragai M., Lammers C.-H., Takeuchi S., Imafuku I., Udagawa Y., Kanazawa I., Kawabata M., Mouradian M.M., Okazawa H.
    Hum. Mol. Genet. 8:977-987(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PQBP1.
    Tissue: Brain.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-341, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.

Entry informationi

Entry nameiPO3F2_HUMAN
AccessioniPrimary (citable) accession number: P20265
Secondary accession number(s): Q14960, Q86V54, Q9UJL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: September 13, 2005
Last modified: July 9, 2014
This is version 156 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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