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Protein

POU domain, class 3, transcription factor 2

Gene

POU3F2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi354 – 413HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

SignaLinkiP20265
SIGNORiP20265

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 3, transcription factor 2
Alternative name(s):
Brain-specific homeobox/POU domain protein 2
Short name:
Brain-2
Short name:
Brn-2
Nervous system-specific octamer-binding transcription factor N-Oct-3
Octamer-binding protein 7
Short name:
Oct-7
Octamer-binding transcription factor 7
Short name:
OTF-7
Gene namesi
Name:POU3F2
Synonyms:BRN2, OCT7, OTF7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000184486.8
HGNCiHGNC:9215 POU3F2
MIMi600494 gene
neXtProtiNX_P20265

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5454
OpenTargetsiENSG00000184486
PharmGKBiPA33539

Chemistry databases

ChEMBLiCHEMBL4189

Polymorphism and mutation databases

BioMutaiPOU3F2
DMDMi78100757

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000134951 – 443POU domain, class 3, transcription factor 2Add BLAST443

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei341PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP20265
PaxDbiP20265
PeptideAtlasiP20265
PRIDEiP20265

PTM databases

iPTMnetiP20265
PhosphoSitePlusiP20265

Expressioni

Tissue specificityi

Expressed specifically in the neuroectodermal cell lineage.

Gene expression databases

BgeeiENSG00000184486
CleanExiHS_POU3F2
GenevisibleiP20265 HS

Organism-specific databases

HPAiHPA047081
HPA056261

Interactioni

Subunit structurei

Interacts with PQBP1 (PubMed:10332029). Interaction with ISL1 (By similarity).By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi1114508 interactors.
IntActiP20265 6 interactors.
STRINGi9606.ENSP00000329170

Chemistry databases

BindingDBiP20265

Structurei

3D structure databases

ProteinModelPortaliP20265
SMRiP20265
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini262 – 336POU-specificPROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi68 – 90Poly-GlyAdd BLAST23
Compositional biasi125 – 149Poly-GlnAdd BLAST25

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802 Eukaryota
ENOG410XQ7X LUCA
GeneTreeiENSGT00760000118935
HOGENOMiHOG000116303
HOVERGENiHBG053120
InParanoidiP20265
KOiK09365
OMAiSFTVNGM
OrthoDBiEOG091G0U0S
PhylomeDBiP20265
TreeFamiTF316413

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
IPR013847 POU
IPR000327 POU_dom
IPR016362 TF_POU_3
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00157 Pou, 1 hit
PIRSFiPIRSF002629 Transcription_factor_POU, 1 hit
PRINTSiPR00028 POUDOMAIN
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00352 POU, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00035 POU_1, 1 hit
PS00465 POU_2, 1 hit
PS51179 POU_3, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform N-OCT 3 (identifier: P20265-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL VQGDYGALQS
60 70 80 90 100
NGHPLSHAHQ WITALSHGGG GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ
110 120 130 140 150
PDIKPSVVVQ QGGRGDELHG PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQR
160 170 180 190 200
PPHLVHHAAN HHPGPGAWRS AAAAAHLPPS MGASNGGLLY SQPSFTVNGM
210 220 230 240 250
LGAGGQPAGL HHHGLRDAHD EPHHADHHPH PHSHPHQQPP PPPPPQGPPG
260 270 280 290 300
HPGAHHDPHS DEDTPTSDDL EQFAKQFKQR RIKLGFTQAD VGLALGTLYG
310 320 330 340 350
NVFSQTTICR FEALQLSFKN MCKLKPLLNK WLEEADSSSG SPTSIDKIAA
360 370 380 390 400
QGRKRKKRTS IEVSVKGALE SHFLKCPKPS AQEITSLADS LQLEKEVVRV
410 420 430 440
WFCNRRQKEK RMTPPGGTLP GAEDVYGGSR DTPPHHGVQT PVQ
Length:443
Mass (Da):46,893
Last modified:September 13, 2005 - v4
Checksum:iD470360894D788C6
GO
Isoform N-OCT 5A (identifier: P20265-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-180: Missing.

Show »
Length:263
Mass (Da):28,723
Checksum:i8E6F82E65137A377
GO
Isoform N-OCT 5B (identifier: P20265-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-199: Missing.

Show »
Length:244
Mass (Da):26,841
Checksum:iD185AA0BBD6C33C7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26G → A in CAA77990 (PubMed:8441633).Curated1
Sequence conflicti170S → T (PubMed:8441633).Curated1
Sequence conflicti170S → T (PubMed:7651733).Curated1
Sequence conflicti207P → S in AAH51699 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0187501 – 199Missing in isoform N-OCT 5B. CuratedAdd BLAST199
Alternative sequenceiVSP_0187491 – 180Missing in isoform N-OCT 5A. CuratedAdd BLAST180

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11933 mRNA Translation: CAA77990.1
L37868 Genomic DNA Translation: AAB59611.1
AL022395 Genomic DNA No translation available.
BC051699 mRNA Translation: AAH51699.2
CCDSiCCDS5040.1 [P20265-1]
PIRiS29334
RefSeqiNP_005595.2, NM_005604.3 [P20265-1]
UniGeneiHs.182505

Genome annotation databases

EnsembliENST00000328345; ENSP00000329170; ENSG00000184486 [P20265-1]
GeneIDi5454
KEGGihsa:5454
UCSCiuc003ppe.4 human [P20265-1]

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiPO3F2_HUMAN
AccessioniPrimary (citable) accession number: P20265
Secondary accession number(s): Q14960, Q86V54, Q9UJL0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: September 13, 2005
Last modified: March 28, 2018
This is version 187 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome