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P20265

- PO3F2_HUMAN

UniProt

P20265 - PO3F2_HUMAN

Protein

POU domain, class 3, transcription factor 2

Gene

POU3F2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 157 (01 Oct 2014)
      Sequence version 4 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi354 – 41360HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct
    3. RNA polymerase II transcription coactivator activity Source: Ensembl
    4. sequence-specific DNA binding Source: Ensembl
    5. sequence-specific DNA binding transcription factor activity Source: ProtInc

    GO - Biological processi

    1. astrocyte development Source: Ensembl
    2. cellular response to organic substance Source: Ensembl
    3. cerebral cortex radially oriented cell migration Source: Ensembl
    4. epidermis development Source: Ensembl
    5. forebrain ventricular zone progenitor cell division Source: Ensembl
    6. hypothalamus cell differentiation Source: Ensembl
    7. myelination in peripheral nervous system Source: Ensembl
    8. neurohypophysis development Source: Ensembl
    9. neuron differentiation Source: Ensembl
    10. positive regulation of cell proliferation Source: MGI
    11. positive regulation of multicellular organism growth Source: Ensembl
    12. regulation of axonogenesis Source: Ensembl

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    SignaLinkiP20265.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    POU domain, class 3, transcription factor 2
    Alternative name(s):
    Brain-specific homeobox/POU domain protein 2
    Short name:
    Brain-2
    Short name:
    Brn-2
    Nervous system-specific octamer-binding transcription factor N-Oct-3
    Octamer-binding protein 7
    Short name:
    Oct-7
    Octamer-binding transcription factor 7
    Short name:
    OTF-7
    Gene namesi
    Name:POU3F2
    Synonyms:BRN2, OCT7, OTF7
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:9215. POU3F2.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. transcription factor complex Source: Ensembl

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33539.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 443443POU domain, class 3, transcription factor 2PRO_0000013495Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei341 – 3411Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP20265.
    PaxDbiP20265.
    PRIDEiP20265.

    PTM databases

    PhosphoSiteiP20265.

    Expressioni

    Tissue specificityi

    Expressed specifically in the neuroectodermal cell lineage.

    Gene expression databases

    BgeeiP20265.
    CleanExiHS_POU3F2.
    GenevestigatoriP20265.

    Interactioni

    Subunit structurei

    Interacts with PQBP1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-1167176,EBI-1167176
    EP300Q094723EBI-1167176,EBI-447295
    PAX3P237602EBI-1167176,EBI-1167564
    SOX10P566933EBI-1167176,EBI-1167533
    TBPP202262EBI-1167176,EBI-355371

    Protein-protein interaction databases

    BioGridi111450. 9 interactions.
    IntActiP20265. 6 interactions.
    MINTiMINT-2801637.
    STRINGi9606.ENSP00000329170.

    Structurei

    3D structure databases

    ProteinModelPortaliP20265.
    SMRiP20265. Positions 265-414.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini262 – 33675POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi68 – 9023Poly-GlyAdd
    BLAST
    Compositional biasi125 – 14925Poly-GlnAdd
    BLAST

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG261729.
    HOGENOMiHOG000116303.
    HOVERGENiHBG053120.
    InParanoidiP20265.
    KOiK09365.
    OMAiMHNSSNN.
    OrthoDBiEOG7DJSMG.
    PhylomeDBiP20265.
    TreeFamiTF316413.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    IPR016362. Transcription_factor_POU.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
    PRINTSiPR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative initiation. Align

    Isoform N-OCT 3 (identifier: P20265-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL VQGDYGALQS    50
    NGHPLSHAHQ WITALSHGGG GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ 100
    PDIKPSVVVQ QGGRGDELHG PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQR 150
    PPHLVHHAAN HHPGPGAWRS AAAAAHLPPS MGASNGGLLY SQPSFTVNGM 200
    LGAGGQPAGL HHHGLRDAHD EPHHADHHPH PHSHPHQQPP PPPPPQGPPG 250
    HPGAHHDPHS DEDTPTSDDL EQFAKQFKQR RIKLGFTQAD VGLALGTLYG 300
    NVFSQTTICR FEALQLSFKN MCKLKPLLNK WLEEADSSSG SPTSIDKIAA 350
    QGRKRKKRTS IEVSVKGALE SHFLKCPKPS AQEITSLADS LQLEKEVVRV 400
    WFCNRRQKEK RMTPPGGTLP GAEDVYGGSR DTPPHHGVQT PVQ 443
    Length:443
    Mass (Da):46,893
    Last modified:September 13, 2005 - v4
    Checksum:iD470360894D788C6
    GO
    Isoform N-OCT 5A (identifier: P20265-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-180: Missing.

    Show »
    Length:263
    Mass (Da):28,723
    Checksum:i8E6F82E65137A377
    GO
    Isoform N-OCT 5B (identifier: P20265-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-199: Missing.

    Show »
    Length:244
    Mass (Da):26,841
    Checksum:iD185AA0BBD6C33C7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 261G → A in CAA77990. (PubMed:8441633)Curated
    Sequence conflicti170 – 1701S → T(PubMed:8441633)Curated
    Sequence conflicti170 – 1701S → T(PubMed:7651733)Curated
    Sequence conflicti207 – 2071P → S in AAH51699. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 199199Missing in isoform N-OCT 5B. CuratedVSP_018750Add
    BLAST
    Alternative sequencei1 – 180180Missing in isoform N-OCT 5A. CuratedVSP_018749Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11933 mRNA. Translation: CAA77990.1.
    L37868 Genomic DNA. Translation: AAB59611.1.
    AL022395 Genomic DNA. Translation: CAB37982.1.
    BC051699 mRNA. Translation: AAH51699.2.
    CCDSiCCDS5040.1. [P20265-1]
    PIRiS29334.
    RefSeqiNP_005595.2. NM_005604.3. [P20265-1]
    UniGeneiHs.182505.

    Genome annotation databases

    EnsembliENST00000328345; ENSP00000329170; ENSG00000184486. [P20265-1]
    GeneIDi5454.
    KEGGihsa:5454.
    UCSCiuc003ppe.3. human. [P20265-1]

    Polymorphism databases

    DMDMi78100757.

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11933 mRNA. Translation: CAA77990.1 .
    L37868 Genomic DNA. Translation: AAB59611.1 .
    AL022395 Genomic DNA. Translation: CAB37982.1 .
    BC051699 mRNA. Translation: AAH51699.2 .
    CCDSi CCDS5040.1. [P20265-1 ]
    PIRi S29334.
    RefSeqi NP_005595.2. NM_005604.3. [P20265-1 ]
    UniGenei Hs.182505.

    3D structure databases

    ProteinModelPortali P20265.
    SMRi P20265. Positions 265-414.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111450. 9 interactions.
    IntActi P20265. 6 interactions.
    MINTi MINT-2801637.
    STRINGi 9606.ENSP00000329170.

    Chemistry

    BindingDBi P20265.
    ChEMBLi CHEMBL4189.

    PTM databases

    PhosphoSitei P20265.

    Polymorphism databases

    DMDMi 78100757.

    Proteomic databases

    MaxQBi P20265.
    PaxDbi P20265.
    PRIDEi P20265.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000328345 ; ENSP00000329170 ; ENSG00000184486 . [P20265-1 ]
    GeneIDi 5454.
    KEGGi hsa:5454.
    UCSCi uc003ppe.3. human. [P20265-1 ]

    Organism-specific databases

    CTDi 5454.
    GeneCardsi GC06P099295.
    HGNCi HGNC:9215. POU3F2.
    MIMi 600494. gene.
    neXtProti NX_P20265.
    PharmGKBi PA33539.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG261729.
    HOGENOMi HOG000116303.
    HOVERGENi HBG053120.
    InParanoidi P20265.
    KOi K09365.
    OMAi MHNSSNN.
    OrthoDBi EOG7DJSMG.
    PhylomeDBi P20265.
    TreeFami TF316413.

    Enzyme and pathway databases

    SignaLinki P20265.

    Miscellaneous databases

    GeneWikii POU3F2.
    GenomeRNAii 5454.
    NextBioi 21109.
    PROi P20265.
    SOURCEi Search...

    Gene expression databases

    Bgeei P20265.
    CleanExi HS_POU3F2.
    Genevestigatori P20265.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    IPR016362. Transcription_factor_POU.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002629. Transcription_factor_POU. 1 hit.
    PRINTSi PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning of human N-Oct3, a nervous-system specific POU domain transcription factor binding to the octamer DNA motif."
      Schreiber E., Tobler A., Malipiero U., Schaffner W., Fontana A.
      Nucleic Acids Res. 21:253-258(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "The brn-2 gene regulates the melanocytic phenotype and tumorigenic potential of human melanoma cells."
      Angus J., Thomson F., Murphy K., Baker E., Sutherland G.R., Parsons P.G., Sturm R.A.
      Oncogene 11:691-700(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Liver.
    3. "The DNA sequence and analysis of human chromosome 6."
      Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
      Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    5. "Expression of a large family of POU-domain regulatory genes in mammalian brain development."
      He X., Treacy M.N., Simmons D.M., Ingraham H.A., Swanson L.W., Rosenfeld M.G.
      Nature 340:35-42(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 280-404.
      Tissue: Brain.
    6. "PQBP-1, a novel polyglutamine tract binding protein, inhibits transcription activation by Brn-2 and affects cell survival."
      Waragai M., Lammers C.-H., Takeuchi S., Imafuku I., Udagawa Y., Kanazawa I., Kawabata M., Mouradian M.M., Okazawa H.
      Hum. Mol. Genet. 8:977-987(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PQBP1.
      Tissue: Brain.
    7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-341, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.

    Entry informationi

    Entry nameiPO3F2_HUMAN
    AccessioniPrimary (citable) accession number: P20265
    Secondary accession number(s): Q14960, Q86V54, Q9UJL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 157 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3