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Protein

POU domain, class 3, transcription factor 2

Gene

POU3F2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi354 – 413HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:G66-33241-MONOMER.
SignaLinkiP20265.
SIGNORiP20265.

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 3, transcription factor 2
Alternative name(s):
Brain-specific homeobox/POU domain protein 2
Short name:
Brain-2
Short name:
Brn-2
Nervous system-specific octamer-binding transcription factor N-Oct-3
Octamer-binding protein 7
Short name:
Oct-7
Octamer-binding transcription factor 7
Short name:
OTF-7
Gene namesi
Name:POU3F2
Synonyms:BRN2, OCT7, OTF7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:9215. POU3F2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5454.
OpenTargetsiENSG00000184486.
PharmGKBiPA33539.

Chemistry databases

ChEMBLiCHEMBL4189.

Polymorphism and mutation databases

BioMutaiPOU3F2.
DMDMi78100757.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000134951 – 443POU domain, class 3, transcription factor 2Add BLAST443

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei341PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP20265.
PaxDbiP20265.
PeptideAtlasiP20265.
PRIDEiP20265.

PTM databases

iPTMnetiP20265.
PhosphoSitePlusiP20265.

Expressioni

Tissue specificityi

Expressed specifically in the neuroectodermal cell lineage.

Gene expression databases

BgeeiENSG00000184486.
CleanExiHS_POU3F2.
GenevisibleiP20265. HS.

Organism-specific databases

HPAiHPA047081.

Interactioni

Subunit structurei

Interacts with PQBP1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1167176,EBI-1167176
EP300Q094723EBI-1167176,EBI-447295
PAX3P237602EBI-1167176,EBI-1167564
SOX10P566933EBI-1167176,EBI-1167533
TBPP202262EBI-1167176,EBI-355371

Protein-protein interaction databases

BioGridi111450. 8 interactors.
IntActiP20265. 6 interactors.
MINTiMINT-2801637.
STRINGi9606.ENSP00000329170.

Chemistry databases

BindingDBiP20265.

Structurei

3D structure databases

ProteinModelPortaliP20265.
SMRiP20265.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini262 – 336POU-specificPROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi68 – 90Poly-GlyAdd BLAST23
Compositional biasi125 – 149Poly-GlnAdd BLAST25

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802. Eukaryota.
ENOG410XQ7X. LUCA.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiP20265.
KOiK09365.
OMAiHPASQHQ.
OrthoDBiEOG091G0U0S.
PhylomeDBiP20265.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
IPR016362. TF_POU_3.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform N-OCT 3 (identifier: P20265-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL VQGDYGALQS
60 70 80 90 100
NGHPLSHAHQ WITALSHGGG GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ
110 120 130 140 150
PDIKPSVVVQ QGGRGDELHG PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQR
160 170 180 190 200
PPHLVHHAAN HHPGPGAWRS AAAAAHLPPS MGASNGGLLY SQPSFTVNGM
210 220 230 240 250
LGAGGQPAGL HHHGLRDAHD EPHHADHHPH PHSHPHQQPP PPPPPQGPPG
260 270 280 290 300
HPGAHHDPHS DEDTPTSDDL EQFAKQFKQR RIKLGFTQAD VGLALGTLYG
310 320 330 340 350
NVFSQTTICR FEALQLSFKN MCKLKPLLNK WLEEADSSSG SPTSIDKIAA
360 370 380 390 400
QGRKRKKRTS IEVSVKGALE SHFLKCPKPS AQEITSLADS LQLEKEVVRV
410 420 430 440
WFCNRRQKEK RMTPPGGTLP GAEDVYGGSR DTPPHHGVQT PVQ
Length:443
Mass (Da):46,893
Last modified:September 13, 2005 - v4
Checksum:iD470360894D788C6
GO
Isoform N-OCT 5A (identifier: P20265-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-180: Missing.

Show »
Length:263
Mass (Da):28,723
Checksum:i8E6F82E65137A377
GO
Isoform N-OCT 5B (identifier: P20265-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-199: Missing.

Show »
Length:244
Mass (Da):26,841
Checksum:iD185AA0BBD6C33C7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26G → A in CAA77990 (PubMed:8441633).Curated1
Sequence conflicti170S → T (PubMed:8441633).Curated1
Sequence conflicti170S → T (PubMed:7651733).Curated1
Sequence conflicti207P → S in AAH51699 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0187501 – 199Missing in isoform N-OCT 5B. CuratedAdd BLAST199
Alternative sequenceiVSP_0187491 – 180Missing in isoform N-OCT 5A. CuratedAdd BLAST180

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11933 mRNA. Translation: CAA77990.1.
L37868 Genomic DNA. Translation: AAB59611.1.
AL022395 Genomic DNA. Translation: CAB37982.1.
BC051699 mRNA. Translation: AAH51699.2.
CCDSiCCDS5040.1. [P20265-1]
PIRiS29334.
RefSeqiNP_005595.2. NM_005604.3. [P20265-1]
UniGeneiHs.182505.

Genome annotation databases

EnsembliENST00000328345; ENSP00000329170; ENSG00000184486. [P20265-1]
GeneIDi5454.
KEGGihsa:5454.
UCSCiuc003ppe.4. human. [P20265-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11933 mRNA. Translation: CAA77990.1.
L37868 Genomic DNA. Translation: AAB59611.1.
AL022395 Genomic DNA. Translation: CAB37982.1.
BC051699 mRNA. Translation: AAH51699.2.
CCDSiCCDS5040.1. [P20265-1]
PIRiS29334.
RefSeqiNP_005595.2. NM_005604.3. [P20265-1]
UniGeneiHs.182505.

3D structure databases

ProteinModelPortaliP20265.
SMRiP20265.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111450. 8 interactors.
IntActiP20265. 6 interactors.
MINTiMINT-2801637.
STRINGi9606.ENSP00000329170.

Chemistry databases

BindingDBiP20265.
ChEMBLiCHEMBL4189.

PTM databases

iPTMnetiP20265.
PhosphoSitePlusiP20265.

Polymorphism and mutation databases

BioMutaiPOU3F2.
DMDMi78100757.

Proteomic databases

MaxQBiP20265.
PaxDbiP20265.
PeptideAtlasiP20265.
PRIDEiP20265.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000328345; ENSP00000329170; ENSG00000184486. [P20265-1]
GeneIDi5454.
KEGGihsa:5454.
UCSCiuc003ppe.4. human. [P20265-1]

Organism-specific databases

CTDi5454.
DisGeNETi5454.
GeneCardsiPOU3F2.
HGNCiHGNC:9215. POU3F2.
HPAiHPA047081.
MIMi600494. gene.
neXtProtiNX_P20265.
OpenTargetsiENSG00000184486.
PharmGKBiPA33539.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3802. Eukaryota.
ENOG410XQ7X. LUCA.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiP20265.
KOiK09365.
OMAiHPASQHQ.
OrthoDBiEOG091G0U0S.
PhylomeDBiP20265.
TreeFamiTF316413.

Enzyme and pathway databases

BioCyciZFISH:G66-33241-MONOMER.
SignaLinkiP20265.
SIGNORiP20265.

Miscellaneous databases

ChiTaRSiPOU3F2. human.
GeneWikiiPOU3F2.
GenomeRNAii5454.
PROiP20265.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000184486.
CleanExiHS_POU3F2.
GenevisibleiP20265. HS.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
IPR016362. TF_POU_3.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPO3F2_HUMAN
AccessioniPrimary (citable) accession number: P20265
Secondary accession number(s): Q14960, Q86V54, Q9UJL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: September 13, 2005
Last modified: November 2, 2016
This is version 175 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.