Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

POU domain, class 3, transcription factor 2

Gene

POU3F2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi354 – 41360HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. RNA polymerase II transcription coactivator activity Source: Ensembl
  3. sequence-specific DNA binding Source: Ensembl
  4. sequence-specific DNA binding transcription factor activity Source: ProtInc

GO - Biological processi

  1. astrocyte development Source: Ensembl
  2. cellular response to organic substance Source: Ensembl
  3. cerebral cortex radially oriented cell migration Source: Ensembl
  4. epidermis development Source: Ensembl
  5. forebrain ventricular zone progenitor cell division Source: Ensembl
  6. hypothalamus cell differentiation Source: Ensembl
  7. myelination in peripheral nervous system Source: Ensembl
  8. neurohypophysis development Source: Ensembl
  9. neuron differentiation Source: Ensembl
  10. positive regulation of cell proliferation Source: MGI
  11. positive regulation of multicellular organism growth Source: Ensembl
  12. regulation of axonogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiP20265.

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 3, transcription factor 2
Alternative name(s):
Brain-specific homeobox/POU domain protein 2
Short name:
Brain-2
Short name:
Brn-2
Nervous system-specific octamer-binding transcription factor N-Oct-3
Octamer-binding protein 7
Short name:
Oct-7
Octamer-binding transcription factor 7
Short name:
OTF-7
Gene namesi
Name:POU3F2
Synonyms:BRN2, OCT7, OTF7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:9215. POU3F2.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
  2. transcription factor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33539.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443POU domain, class 3, transcription factor 2PRO_0000013495Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei341 – 3411Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP20265.
PaxDbiP20265.
PRIDEiP20265.

PTM databases

PhosphoSiteiP20265.

Expressioni

Tissue specificityi

Expressed specifically in the neuroectodermal cell lineage.

Gene expression databases

BgeeiP20265.
CleanExiHS_POU3F2.
GenevestigatoriP20265.

Interactioni

Subunit structurei

Interacts with PQBP1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1167176,EBI-1167176
EP300Q094723EBI-1167176,EBI-447295
PAX3P237602EBI-1167176,EBI-1167564
SOX10P566933EBI-1167176,EBI-1167533
TBPP202262EBI-1167176,EBI-355371

Protein-protein interaction databases

BioGridi111450. 9 interactions.
IntActiP20265. 6 interactions.
MINTiMINT-2801637.
STRINGi9606.ENSP00000329170.

Structurei

3D structure databases

ProteinModelPortaliP20265.
SMRiP20265. Positions 265-414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini262 – 33675POU-specificPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi68 – 9023Poly-GlyAdd
BLAST
Compositional biasi125 – 14925Poly-GlnAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG261729.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiP20265.
KOiK09365.
OMAiMHNSSNN.
OrthoDBiEOG7DJSMG.
PhylomeDBiP20265.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. TF_POU_3.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform N-OCT 3 (identifier: P20265-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL VQGDYGALQS
60 70 80 90 100
NGHPLSHAHQ WITALSHGGG GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ
110 120 130 140 150
PDIKPSVVVQ QGGRGDELHG PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQR
160 170 180 190 200
PPHLVHHAAN HHPGPGAWRS AAAAAHLPPS MGASNGGLLY SQPSFTVNGM
210 220 230 240 250
LGAGGQPAGL HHHGLRDAHD EPHHADHHPH PHSHPHQQPP PPPPPQGPPG
260 270 280 290 300
HPGAHHDPHS DEDTPTSDDL EQFAKQFKQR RIKLGFTQAD VGLALGTLYG
310 320 330 340 350
NVFSQTTICR FEALQLSFKN MCKLKPLLNK WLEEADSSSG SPTSIDKIAA
360 370 380 390 400
QGRKRKKRTS IEVSVKGALE SHFLKCPKPS AQEITSLADS LQLEKEVVRV
410 420 430 440
WFCNRRQKEK RMTPPGGTLP GAEDVYGGSR DTPPHHGVQT PVQ
Length:443
Mass (Da):46,893
Last modified:September 13, 2005 - v4
Checksum:iD470360894D788C6
GO
Isoform N-OCT 5A (identifier: P20265-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-180: Missing.

Show »
Length:263
Mass (Da):28,723
Checksum:i8E6F82E65137A377
GO
Isoform N-OCT 5B (identifier: P20265-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-199: Missing.

Show »
Length:244
Mass (Da):26,841
Checksum:iD185AA0BBD6C33C7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261G → A in CAA77990 (PubMed:8441633).Curated
Sequence conflicti170 – 1701S → T (PubMed:8441633).Curated
Sequence conflicti170 – 1701S → T (PubMed:7651733).Curated
Sequence conflicti207 – 2071P → S in AAH51699 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 199199Missing in isoform N-OCT 5B. CuratedVSP_018750Add
BLAST
Alternative sequencei1 – 180180Missing in isoform N-OCT 5A. CuratedVSP_018749Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11933 mRNA. Translation: CAA77990.1.
L37868 Genomic DNA. Translation: AAB59611.1.
AL022395 Genomic DNA. Translation: CAB37982.1.
BC051699 mRNA. Translation: AAH51699.2.
CCDSiCCDS5040.1. [P20265-1]
PIRiS29334.
RefSeqiNP_005595.2. NM_005604.3. [P20265-1]
UniGeneiHs.182505.

Genome annotation databases

EnsembliENST00000328345; ENSP00000329170; ENSG00000184486. [P20265-1]
GeneIDi5454.
KEGGihsa:5454.
UCSCiuc003ppe.3. human. [P20265-1]

Polymorphism databases

DMDMi78100757.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11933 mRNA. Translation: CAA77990.1.
L37868 Genomic DNA. Translation: AAB59611.1.
AL022395 Genomic DNA. Translation: CAB37982.1.
BC051699 mRNA. Translation: AAH51699.2.
CCDSiCCDS5040.1. [P20265-1]
PIRiS29334.
RefSeqiNP_005595.2. NM_005604.3. [P20265-1]
UniGeneiHs.182505.

3D structure databases

ProteinModelPortaliP20265.
SMRiP20265. Positions 265-414.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111450. 9 interactions.
IntActiP20265. 6 interactions.
MINTiMINT-2801637.
STRINGi9606.ENSP00000329170.

Chemistry

BindingDBiP20265.
ChEMBLiCHEMBL4189.

PTM databases

PhosphoSiteiP20265.

Polymorphism databases

DMDMi78100757.

Proteomic databases

MaxQBiP20265.
PaxDbiP20265.
PRIDEiP20265.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000328345; ENSP00000329170; ENSG00000184486. [P20265-1]
GeneIDi5454.
KEGGihsa:5454.
UCSCiuc003ppe.3. human. [P20265-1]

Organism-specific databases

CTDi5454.
GeneCardsiGC06P099295.
HGNCiHGNC:9215. POU3F2.
MIMi600494. gene.
neXtProtiNX_P20265.
PharmGKBiPA33539.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG261729.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiP20265.
KOiK09365.
OMAiMHNSSNN.
OrthoDBiEOG7DJSMG.
PhylomeDBiP20265.
TreeFamiTF316413.

Enzyme and pathway databases

SignaLinkiP20265.

Miscellaneous databases

ChiTaRSiPOU3F2. human.
GeneWikiiPOU3F2.
GenomeRNAii5454.
NextBioi21109.
PROiP20265.
SOURCEiSearch...

Gene expression databases

BgeeiP20265.
CleanExiHS_POU3F2.
GenevestigatoriP20265.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. TF_POU_3.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning of human N-Oct3, a nervous-system specific POU domain transcription factor binding to the octamer DNA motif."
    Schreiber E., Tobler A., Malipiero U., Schaffner W., Fontana A.
    Nucleic Acids Res. 21:253-258(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The brn-2 gene regulates the melanocytic phenotype and tumorigenic potential of human melanoma cells."
    Angus J., Thomson F., Murphy K., Baker E., Sutherland G.R., Parsons P.G., Sturm R.A.
    Oncogene 11:691-700(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  3. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. "Expression of a large family of POU-domain regulatory genes in mammalian brain development."
    He X., Treacy M.N., Simmons D.M., Ingraham H.A., Swanson L.W., Rosenfeld M.G.
    Nature 340:35-42(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 280-404.
    Tissue: Brain.
  6. "PQBP-1, a novel polyglutamine tract binding protein, inhibits transcription activation by Brn-2 and affects cell survival."
    Waragai M., Lammers C.-H., Takeuchi S., Imafuku I., Udagawa Y., Kanazawa I., Kawabata M., Mouradian M.M., Okazawa H.
    Hum. Mol. Genet. 8:977-987(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PQBP1.
    Tissue: Brain.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-341, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.

Entry informationi

Entry nameiPO3F2_HUMAN
AccessioniPrimary (citable) accession number: P20265
Secondary accession number(s): Q14960, Q86V54, Q9UJL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: September 13, 2005
Last modified: April 1, 2015
This is version 161 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.