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P20265 (PO3F2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 153. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 3, transcription factor 2
Alternative name(s):
Brain-specific homeobox/POU domain protein 2
Short name=Brain-2
Short name=Brn-2
Nervous system-specific octamer-binding transcription factor N-Oct-3
Octamer-binding protein 7
Short name=Oct-7
Octamer-binding transcription factor 7
Short name=OTF-7
Gene names
Name:POU3F2
Synonyms:BRN2, OCT7, OTF7
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length443 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters By similarity.

Subunit structure

Interacts with PQBP1. Ref.6

Subcellular location

Nucleus.

Tissue specificity

Expressed specifically in the neuroectodermal cell lineage.

Sequence similarities

Belongs to the POU transcription factor family. Class-3 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative initiation
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processastrocyte development

Inferred from electronic annotation. Source: Ensembl

cellular response to organic substance

Inferred from electronic annotation. Source: Ensembl

cerebral cortex radially oriented cell migration

Inferred from electronic annotation. Source: Ensembl

epidermis development

Inferred from electronic annotation. Source: Ensembl

forebrain ventricular zone progenitor cell division

Inferred from electronic annotation. Source: Ensembl

hypothalamus cell differentiation

Inferred from electronic annotation. Source: Ensembl

myelination in peripheral nervous system

Inferred from electronic annotation. Source: Ensembl

neurohypophysis development

Inferred from electronic annotation. Source: Ensembl

neuron differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from direct assay PubMed 15024079. Source: MGI

positive regulation of multicellular organism growth

Inferred from electronic annotation. Source: Ensembl

regulation of axonogenesis

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

transcription factor complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionRNA polymerase II transcription coactivator activity

Inferred from electronic annotation. Source: Ensembl

identical protein binding

Inferred from physical interaction PubMed 11029584. Source: IntAct

sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

sequence-specific DNA binding transcription factor activity

Traceable author statement PubMed 7601453PubMed 9482665. Source: ProtInc

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative initiation. [Align] [Select]
Isoform N-OCT 3 (identifier: P20265-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform N-OCT 5A (identifier: P20265-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-180: Missing.
Isoform N-OCT 5B (identifier: P20265-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-199: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 443443POU domain, class 3, transcription factor 2
PRO_0000013495

Regions

Domain262 – 33675POU-specific
DNA binding354 – 41360Homeobox
Compositional bias68 – 9023Poly-Gly
Compositional bias125 – 14925Poly-Gln

Amino acid modifications

Modified residue3411Phosphoserine Ref.8

Natural variations

Alternative sequence1 – 199199Missing in isoform N-OCT 5B.
VSP_018750
Alternative sequence1 – 180180Missing in isoform N-OCT 5A.
VSP_018749

Experimental info

Sequence conflict261G → A in CAA77990. Ref.1
Sequence conflict1701S → T Ref.1
Sequence conflict1701S → T Ref.2
Sequence conflict2071P → S in AAH51699. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform N-OCT 3 [UniParc].

Last modified September 13, 2005. Version 4.
Checksum: D470360894D788C6

FASTA44346,893
        10         20         30         40         50         60 
MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL VQGDYGALQS NGHPLSHAHQ 

        70         80         90        100        110        120 
WITALSHGGG GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ PDIKPSVVVQ QGGRGDELHG 

       130        140        150        160        170        180 
PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQR PPHLVHHAAN HHPGPGAWRS AAAAAHLPPS 

       190        200        210        220        230        240 
MGASNGGLLY SQPSFTVNGM LGAGGQPAGL HHHGLRDAHD EPHHADHHPH PHSHPHQQPP 

       250        260        270        280        290        300 
PPPPPQGPPG HPGAHHDPHS DEDTPTSDDL EQFAKQFKQR RIKLGFTQAD VGLALGTLYG 

       310        320        330        340        350        360 
NVFSQTTICR FEALQLSFKN MCKLKPLLNK WLEEADSSSG SPTSIDKIAA QGRKRKKRTS 

       370        380        390        400        410        420 
IEVSVKGALE SHFLKCPKPS AQEITSLADS LQLEKEVVRV WFCNRRQKEK RMTPPGGTLP 

       430        440 
GAEDVYGGSR DTPPHHGVQT PVQ 

« Hide

Isoform N-OCT 5A [UniParc].

Checksum: 8E6F82E65137A377
Show »

FASTA26328,723
Isoform N-OCT 5B [UniParc].

Checksum: D185AA0BBD6C33C7
Show »

FASTA24426,841

References

« Hide 'large scale' references
[1]"cDNA cloning of human N-Oct3, a nervous-system specific POU domain transcription factor binding to the octamer DNA motif."
Schreiber E., Tobler A., Malipiero U., Schaffner W., Fontana A.
Nucleic Acids Res. 21:253-258(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"The brn-2 gene regulates the melanocytic phenotype and tumorigenic potential of human melanoma cells."
Angus J., Thomson F., Murphy K., Baker E., Sutherland G.R., Parsons P.G., Sturm R.A.
Oncogene 11:691-700(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Liver.
[3]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"Expression of a large family of POU-domain regulatory genes in mammalian brain development."
He X., Treacy M.N., Simmons D.M., Ingraham H.A., Swanson L.W., Rosenfeld M.G.
Nature 340:35-42(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 280-404.
Tissue: Brain.
[6]"PQBP-1, a novel polyglutamine tract binding protein, inhibits transcription activation by Brn-2 and affects cell survival."
Waragai M., Lammers C.-H., Takeuchi S., Imafuku I., Udagawa Y., Kanazawa I., Kawabata M., Mouradian M.M., Okazawa H.
Hum. Mol. Genet. 8:977-987(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PQBP1.
Tissue: Brain.
[7]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-341, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z11933 mRNA. Translation: CAA77990.1.
L37868 Genomic DNA. Translation: AAB59611.1.
AL022395 Genomic DNA. Translation: CAB37982.1.
BC051699 mRNA. Translation: AAH51699.2.
PIRS29334.
RefSeqNP_005595.2. NM_005604.3.
UniGeneHs.182505.

3D structure databases

ProteinModelPortalP20265.
SMRP20265. Positions 265-414.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111450. 9 interactions.
IntActP20265. 6 interactions.
MINTMINT-2801637.
STRING9606.ENSP00000329170.

Chemistry

BindingDBP20265.
ChEMBLCHEMBL4189.

PTM databases

PhosphoSiteP20265.

Polymorphism databases

DMDM78100757.

Proteomic databases

PaxDbP20265.
PRIDEP20265.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000328345; ENSP00000329170; ENSG00000184486. [P20265-1]
GeneID5454.
KEGGhsa:5454.
UCSCuc003ppe.3. human. [P20265-1]

Organism-specific databases

CTD5454.
GeneCardsGC06P099295.
HGNCHGNC:9215. POU3F2.
MIM600494. gene.
neXtProtNX_P20265.
PharmGKBPA33539.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG261729.
HOGENOMHOG000116303.
HOVERGENHBG053120.
InParanoidP20265.
KOK09365.
OMAMHNSSNN.
OrthoDBEOG7DJSMG.
PhylomeDBP20265.
TreeFamTF316413.

Enzyme and pathway databases

SignaLinkP20265.

Gene expression databases

BgeeP20265.
CleanExHS_POU3F2.
GenevestigatorP20265.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. Transcription_factor_POU.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiPOU3F2.
GenomeRNAi5454.
NextBio21109.
PROP20265.
SOURCESearch...

Entry information

Entry namePO3F2_HUMAN
AccessionPrimary (citable) accession number: P20265
Secondary accession number(s): Q14960, Q86V54, Q9UJL0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: September 13, 2005
Last modified: April 16, 2014
This is version 153 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM