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Protein

POU domain, class 3, transcription factor 3

Gene

POU3F3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3') (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi406 – 46560HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • sequence-specific DNA binding Source: UniProtKB
  • sequence-specific DNA binding transcription factor activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 3, transcription factor 3
Alternative name(s):
Brain-specific homeobox/POU domain protein 1
Short name:
Brain-1
Short name:
Brn-1
Octamer-binding protein 8
Short name:
Oct-8
Octamer-binding transcription factor 8
Short name:
OTF-8
Gene namesi
Name:POU3F3
Synonyms:BRN1, OTF8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:9216. POU3F3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33540.

Polymorphism and mutation databases

BioMutaiPOU3F3.
DMDMi2506534.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500POU domain, class 3, transcription factor 3PRO_0000100727Add
BLAST

Proteomic databases

MaxQBiP20264.
PaxDbiP20264.
PRIDEiP20264.

PTM databases

PhosphoSiteiP20264.

Expressioni

Tissue specificityi

Brain.

Gene expression databases

BgeeiP20264.
CleanExiHS_POU3F3.
GenevisibleiP20264. HS.

Interactioni

Protein-protein interaction databases

BioGridi111451. 2 interactions.
IntActiP20264. 2 interactions.
MINTiMINT-1410140.
STRINGi9606.ENSP00000355001.

Structurei

3D structure databases

ProteinModelPortaliP20264.
SMRiP20264. Positions 317-466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini314 – 38875POU-specificPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi28 – 304277Gly-richAdd
BLAST
Compositional biasi81 – 204124Ala-richAdd
BLAST
Compositional biasi133 – 18452Pro-richAdd
BLAST
Compositional biasi270 – 29425His-richAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG261729.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiP20264.
KOiK09365.
OMAiPHQGHPG.
OrthoDBiEOG7DJSMG.
PhylomeDBiP20264.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. TF_POU_3.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20264-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAASNPYL PGNSLLAAGS IVHSDAAGAG GGGGGGGGGG GGGAGGGGGG
60 70 80 90 100
MQPGSAAVTS GAYRGDPSSV KMVQSDFMQG AMAASNGGHM LSHAHQWVTA
110 120 130 140 150
LPHAAAAAAA AAAAAVEASS PWSGSAVGMA GSPQQPPQPP PPPPQGPDVK
160 170 180 190 200
GGAGRDDLHA GTALHHRGPP HLGPPPPPPH QGHPGGWGAA AAAAAAAAAA
210 220 230 240 250
AAAAHLPSMA GGQQPPPQSL LYSQPGGFTV NGMLSAPPGP GGGGGGAGGG
260 270 280 290 300
AQSLVHPGLV RGDTPELAEH HHHHHHHAHP HPPHPHHAQG PPHHGGGGGG
310 320 330 340 350
AGPGLNSHDP HSDEDTPTSD DLEQFAKQFK QRRIKLGFTQ ADVGLALGTL
360 370 380 390 400
YGNVFSQTTI CRFEALQLSF KNMCKLKPLL NKWLEEADSS TGSPTSIDKI
410 420 430 440 450
AAQGRKRKKR TSIEVSVKGA LESHFLKCPK PSAQEITNLA DSLQLEKEVV
460 470 480 490 500
RVWFCNRRQK EKRMTPPGIQ QQTPDDVYSQ VGTVSADTPP PHHGLQTSVQ
Length:500
Mass (Da):50,327
Last modified:November 1, 1997 - v2
Checksum:iE536EFFFA5212319
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti433 – 4331A → S (PubMed:2739723).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001835 Genomic DNA. Translation: BAA19459.1.
AC018730 Genomic DNA. Translation: AAX88973.1.
CCDSiCCDS33265.1.
RefSeqiNP_006227.1. NM_006236.2.
UniGeneiHs.248158.

Genome annotation databases

EnsembliENST00000361360; ENSP00000355001; ENSG00000198914.
GeneIDi5455.
KEGGihsa:5455.
UCSCiuc010ywg.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001835 Genomic DNA. Translation: BAA19459.1.
AC018730 Genomic DNA. Translation: AAX88973.1.
CCDSiCCDS33265.1.
RefSeqiNP_006227.1. NM_006236.2.
UniGeneiHs.248158.

3D structure databases

ProteinModelPortaliP20264.
SMRiP20264. Positions 317-466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111451. 2 interactions.
IntActiP20264. 2 interactions.
MINTiMINT-1410140.
STRINGi9606.ENSP00000355001.

Chemistry

BindingDBiP20264.
ChEMBLiCHEMBL5243.

PTM databases

PhosphoSiteiP20264.

Polymorphism and mutation databases

BioMutaiPOU3F3.
DMDMi2506534.

Proteomic databases

MaxQBiP20264.
PaxDbiP20264.
PRIDEiP20264.

Protocols and materials databases

DNASUi5455.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361360; ENSP00000355001; ENSG00000198914.
GeneIDi5455.
KEGGihsa:5455.
UCSCiuc010ywg.2. human.

Organism-specific databases

CTDi5455.
GeneCardsiGC02P105471.
HGNCiHGNC:9216. POU3F3.
MIMi602480. gene.
neXtProtiNX_P20264.
PharmGKBiPA33540.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG261729.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiP20264.
KOiK09365.
OMAiPHQGHPG.
OrthoDBiEOG7DJSMG.
PhylomeDBiP20264.
TreeFamiTF316413.

Miscellaneous databases

GenomeRNAii5455.
NextBioi21113.
PROiP20264.
SOURCEiSearch...

Gene expression databases

BgeeiP20264.
CleanExiHS_POU3F3.
GenevisibleiP20264. HS.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. TF_POU_3.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Class III POU genes: generation of homopolymeric amino acid repeats under GC pressure in mammals."
    Sumiyama K., Washio-Watanabe K., Saitou N., Hayakawa T., Ueda S.
    J. Mol. Evol. 43:170-178(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Expression of a large family of POU-domain regulatory genes in mammalian brain development."
    He X., Treacy M.N., Simmons D.M., Ingraham H.A., Swanson L.W., Rosenfeld M.G.
    Nature 340:35-42(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 332-456.
    Tissue: Brain.

Entry informationi

Entry nameiPO3F3_HUMAN
AccessioniPrimary (citable) accession number: P20264
Secondary accession number(s): P78379, Q4ZG25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: November 1, 1997
Last modified: July 22, 2015
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.