P20264 (PO3F3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
POU domain, class 3, transcription factor 3

Alternative name(s):
Brain-specific homeobox/POU domain protein 1
Short name=Brain-1
Short name=Brn-1
Octamer-binding protein 8
Short name=Oct-8
Octamer-binding transcription factor 8
Short name=OTF-8

Gene names

Name:	POU3F3
Synonyms:	BRN1, OTF8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3') By similarity.
Subcellular location	Nucleus.
Tissue specificity	Brain.
Sequence similarities	Belongs to the POU transcription factor family. Class-3 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.

Ontologies

Keywords
Biological process	Neurogenesis Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	central nervous system development Traceable author statement Ref.1. Source: ProtInc cerebral cortex radially oriented cell migration Inferred from electronic annotation. Source: Compara forebrain ventricular zone progenitor cell division Inferred from electronic annotation. Source: Compara metanephric DCT cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB metanephric ascending thin limb development Inferred from sequence or structural similarity. Source: UniProtKB metanephric macula densa development Inferred from sequence or structural similarity. Source: UniProtKB metanephric thick ascending limb development Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	sequence-specific DNA binding Inferred from sequence or structural similarity. Source: UniProtKB sequence-specific DNA binding transcription factor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	POU domain, class 3, transcription factor 3		PRO_0000100727
Regions
	Domain	314 – 388	75	POU-specific
	DNA binding	406 – 465	60	Homeobox
	Compositional bias	28 – 304	277	Gly-rich
	Compositional bias	81 – 204	124	Ala-rich
	Compositional bias	133 – 184	52	Pro-rich
	Compositional bias	270 – 294	25	His-rich
Experimental info
	Sequence conflict	433	1	A → S Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P20264 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: E536EFFFA5212319	FASTA	500	50,327
	10 20 30 40 50 60 MATAASNPYL PGNSLLAAGS IVHSDAAGAG GGGGGGGGGG GGGAGGGGGG MQPGSAAVTS 70 80 90 100 110 120 GAYRGDPSSV KMVQSDFMQG AMAASNGGHM LSHAHQWVTA LPHAAAAAAA AAAAAVEASS 130 140 150 160 170 180 PWSGSAVGMA GSPQQPPQPP PPPPQGPDVK GGAGRDDLHA GTALHHRGPP HLGPPPPPPH 190 200 210 220 230 240 QGHPGGWGAA AAAAAAAAAA AAAAHLPSMA GGQQPPPQSL LYSQPGGFTV NGMLSAPPGP 250 260 270 280 290 300 GGGGGGAGGG AQSLVHPGLV RGDTPELAEH HHHHHHHAHP HPPHPHHAQG PPHHGGGGGG 310 320 330 340 350 360 AGPGLNSHDP HSDEDTPTSD DLEQFAKQFK QRRIKLGFTQ ADVGLALGTL YGNVFSQTTI 370 380 390 400 410 420 CRFEALQLSF KNMCKLKPLL NKWLEEADSS TGSPTSIDKI AAQGRKRKKR TSIEVSVKGA 430 440 450 460 470 480 LESHFLKCPK PSAQEITNLA DSLQLEKEVV RVWFCNRRQK EKRMTPPGIQ QQTPDDVYSQ 490 500 VGTVSADTPP PHHGLQTSVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Class III POU genes: generation of homopolymeric amino acid repeats under GC pressure in mammals." Sumiyama K., Washio-Watanabe K., Saitou N., Hayakawa T., Ueda S. J. Mol. Evol. 43:170-178(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Expression of a large family of POU-domain regulatory genes in mammalian brain development." He X., Treacy M.N., Simmons D.M., Ingraham H.A., Swanson L.W., Rosenfeld M.G. Nature 340:35-42(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 332-456. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB001835 Genomic DNA. Translation: BAA19459.1. AC018730 Genomic DNA. Translation: AAX88973.1.
IPI	IPI00022911.
RefSeq	NP_006227.1. NM_006236.1.
UniGene	Hs.673855.
3D structure databases
ProteinModelPortal	P20264.
ModBase	Search...
Protein-protein interaction databases
IntAct	P20264. 1 interaction.
MINT	MINT-1410140.
STRING	9606.ENSP00000355001.
PTM databases
PhosphoSite	P20264.
Polymorphism databases
DMDM	2506534.
Proteomic databases
PaxDb	P20264.
PRIDE	P20264.
Protocols and materials databases
DNASU	5455.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000361360; ENSP00000355001; ENSG00000198914.
GeneID	5455.
KEGG	hsa:5455.
UCSC	uc010ywg.2. human.
Organism-specific databases
CTD	5455.
GeneCards	GC02P105471.
HGNC	HGNC:9216. POU3F3.
MIM	602480. gene.
neXtProt	NX_P20264.
PharmGKB	PA33540.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG261729.
HOGENOM	HOG000116303.
HOVERGEN	HBG053120.
InParanoid	P20264.
KO	K09365.
OMA	HRPPHLG.
OrthoDB	EOG4HDSTS.
Gene expression databases
Bgee	P20264.
CleanEx	HS_POU3F3.
Genevestigator	P20264.
GermOnline	ENSG00000198914. Homo sapiens.
Family and domain databases
Gene3D	1.10.10.60. 1 hit. 1.10.260.40. 1 hit.
InterPro	IPR017970. Homeobox_CS. IPR001356. Homeodomain. IPR009057. Homeodomain-like. IPR010982. Lambda_DNA-bd_dom. IPR013847. POU. IPR000327. POU_specific. IPR016362. Transcription_factor_POU. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. PF00157. Pou. 1 hit. [Graphical view]
PIRSF	PIRSF002629. Transcription_factor_POU. 1 hit.
PRINTS	PR00028. POUDOMAIN.
SMART	SM00389. HOX. 1 hit. SM00352. POU. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit. SSF47413. Lambda_like_DNA. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. PS00035. POU_1. 1 hit. PS00465. POU_2. 1 hit. PS51179. POU_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P20264.
ChEMBL	CHEMBL5243.
GenomeRNAi	5455.
NextBio	21113.
SOURCE	Search...

Entry information

Entry name

PO3F3_HUMAN

Accession

Primary (citable) accession number: P20264
Secondary accession number(s): P78379, Q4ZG25

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.