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P20264 (PO3F3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 3, transcription factor 3
Alternative name(s):
Brain-specific homeobox/POU domain protein 1
Short name=Brain-1
Short name=Brn-1
Octamer-binding protein 8
Short name=Oct-8
Octamer-binding transcription factor 8
Short name=OTF-8
Gene names
Name:POU3F3
Synonyms:BRN1, OTF8
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3') By similarity.

Subcellular location

Nucleus.

Tissue specificity

Brain.

Sequence similarities

Belongs to the POU transcription factor family. Class-3 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Ontologies

Keywords
   Biological processNeurogenesis
Transcription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcentral nervous system development

Traceable author statement Ref.1. Source: ProtInc

cerebral cortex radially oriented cell migration

Inferred from electronic annotation. Source: Ensembl

forebrain ventricular zone progenitor cell division

Inferred from electronic annotation. Source: Ensembl

metanephric DCT cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

metanephric ascending thin limb development

Inferred from sequence or structural similarity. Source: UniProtKB

metanephric loop of Henle development

Inferred from sequence or structural similarity. Source: UniProtKB

metanephric macula densa development

Inferred from sequence or structural similarity. Source: UniProtKB

metanephric thick ascending limb development

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of gene expression

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionsequence-specific DNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Traceable author statement Ref.1. Source: ProtInc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 500500POU domain, class 3, transcription factor 3
PRO_0000100727

Regions

Domain314 – 38875POU-specific
DNA binding406 – 46560Homeobox
Compositional bias28 – 304277Gly-rich
Compositional bias81 – 204124Ala-rich
Compositional bias133 – 18452Pro-rich
Compositional bias270 – 29425His-rich

Experimental info

Sequence conflict4331A → S Ref.3

Sequences

Sequence LengthMass (Da)Tools
P20264 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: E536EFFFA5212319

FASTA50050,327
        10         20         30         40         50         60 
MATAASNPYL PGNSLLAAGS IVHSDAAGAG GGGGGGGGGG GGGAGGGGGG MQPGSAAVTS 

        70         80         90        100        110        120 
GAYRGDPSSV KMVQSDFMQG AMAASNGGHM LSHAHQWVTA LPHAAAAAAA AAAAAVEASS 

       130        140        150        160        170        180 
PWSGSAVGMA GSPQQPPQPP PPPPQGPDVK GGAGRDDLHA GTALHHRGPP HLGPPPPPPH 

       190        200        210        220        230        240 
QGHPGGWGAA AAAAAAAAAA AAAAHLPSMA GGQQPPPQSL LYSQPGGFTV NGMLSAPPGP 

       250        260        270        280        290        300 
GGGGGGAGGG AQSLVHPGLV RGDTPELAEH HHHHHHHAHP HPPHPHHAQG PPHHGGGGGG 

       310        320        330        340        350        360 
AGPGLNSHDP HSDEDTPTSD DLEQFAKQFK QRRIKLGFTQ ADVGLALGTL YGNVFSQTTI 

       370        380        390        400        410        420 
CRFEALQLSF KNMCKLKPLL NKWLEEADSS TGSPTSIDKI AAQGRKRKKR TSIEVSVKGA 

       430        440        450        460        470        480 
LESHFLKCPK PSAQEITNLA DSLQLEKEVV RVWFCNRRQK EKRMTPPGIQ QQTPDDVYSQ 

       490        500 
VGTVSADTPP PHHGLQTSVQ 

« Hide

References

« Hide 'large scale' references
[1]"Class III POU genes: generation of homopolymeric amino acid repeats under GC pressure in mammals."
Sumiyama K., Washio-Watanabe K., Saitou N., Hayakawa T., Ueda S.
J. Mol. Evol. 43:170-178(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Expression of a large family of POU-domain regulatory genes in mammalian brain development."
He X., Treacy M.N., Simmons D.M., Ingraham H.A., Swanson L.W., Rosenfeld M.G.
Nature 340:35-42(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 332-456.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB001835 Genomic DNA. Translation: BAA19459.1.
AC018730 Genomic DNA. Translation: AAX88973.1.
CCDSCCDS33265.1.
RefSeqNP_006227.1. NM_006236.1.
UniGeneHs.673855.

3D structure databases

ProteinModelPortalP20264.
SMRP20264. Positions 317-466.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111451. 2 interactions.
IntActP20264. 2 interactions.
MINTMINT-1410140.
STRING9606.ENSP00000355001.

Chemistry

BindingDBP20264.
ChEMBLCHEMBL5243.

PTM databases

PhosphoSiteP20264.

Polymorphism databases

DMDM2506534.

Proteomic databases

MaxQBP20264.
PaxDbP20264.
PRIDEP20264.

Protocols and materials databases

DNASU5455.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000361360; ENSP00000355001; ENSG00000198914.
GeneID5455.
KEGGhsa:5455.
UCSCuc010ywg.2. human.

Organism-specific databases

CTD5455.
GeneCardsGC02P105471.
HGNCHGNC:9216. POU3F3.
MIM602480. gene.
neXtProtNX_P20264.
PharmGKBPA33540.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG261729.
HOGENOMHOG000116303.
HOVERGENHBG053120.
InParanoidP20264.
KOK09365.
OMAAHLPSMA.
OrthoDBEOG7DJSMG.
PhylomeDBP20264.
TreeFamTF316413.

Gene expression databases

BgeeP20264.
CleanExHS_POU3F3.
GenevestigatorP20264.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. Transcription_factor_POU.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi5455.
NextBio21113.
PROP20264.
SOURCESearch...

Entry information

Entry namePO3F3_HUMAN
AccessionPrimary (citable) accession number: P20264
Secondary accession number(s): P78379, Q4ZG25
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM