Reviewed,
UniProtKB/Swiss-Prot P20263 (PO5F1_MOUSE)
Last modified
November 3, 2009.
Version 101.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: POU domain, class 5, transcription factor 1 Alternative name(s): Octamer-binding transcription factor 3 Short name=Oct-3 Oct-4 NF-A3 | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 352 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Ref.7 Ref.8 |
| Subunit structure | Interacts with PKM2 By similarity. Interacts with UBE2I. |
| Subcellular location | Nucleus. Note: Expressed in a diffuse and slightly punctuate pattern. Ref.7 Ref.8 |
| Tissue specificity | Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo. Also expressed in primordial germ cells and in the female germ line. Absent from adult tissues. Ref.1 Ref.2 |
| Developmental stage | Down-regulated during differentiation to endoderm and mesoderm. Ref.1 |
| Induction | |
| Domain | The POU-specific domain mediates interaction with PKM2 By similarity. |
| Post-translational modification | Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. |
| Sequence similarities | Belongs to the POU transcription factor family. Class-5 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Ewsr1 | Q61545 | 7 | EBI-1606219,EBI-1606991 | |
| Nanog | Q80Z64 | 3 | EBI-1606219,EBI-2312517 | |
| Nr0b1 | Q61066 | 1 | EBI-1606219,EBI-2312665 | |
| Pkm2 | P52480 | 4 | EBI-1606219,EBI-647785 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||
Molecule processing | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 352 | 352 | POU domain, class 5, transcription factor 1 | PRO_0000100749 | ||||||||||||||
Regions | ||||||||||||||||||
| Domain | 131 – 205 | 75 | POU-specific | |||||||||||||||
| DNA binding | 223 – 282 | 60 | Homeobox | |||||||||||||||
Amino acid modifications | ||||||||||||||||||
| Cross-link | 118 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Ref.7 Ref.8 Ref.6 | ||||||||||||||||
Experimental info | ||||||||||||||||||
| Mutagenesis | 118 | 1 | K → R: Absence of sumoylation. Enhanced protein degradation. Reduced self-renewal ability in ES cells. 70% lower expression of YES1. Reduced DNA binding. No change in nuclear location. No change in nuclear localization. Absence of sumoylation; when associated with R-215 and R-244. Ref.7 Ref.8 Ref.6 | |||||||||||||||
| Mutagenesis | 120 | 1 | E → A: Absence of sumoylation. Enhanced protein degradation. Reduced self-renewal ability in ES cells. 55% lower expression of YES1. Ref.7 | |||||||||||||||
| Mutagenesis | 215 | 1 | K → R: No change in sumoylation; when associated with R-244. Loss of sumoylation. No change in nuclear localization; when associated with R-118 and R-244. Ref.7 | |||||||||||||||
| Mutagenesis | 244 | 1 | K → R: No change in sumoylation. No change in sumoylation; when associated with R-215. Loss of sumoylation; when associated with R-118 and R-215. No change in nuclear localization; when associated with R-118 and R-215. Ref.7 | |||||||||||||||
| Sequence conflict | 1 – 28 | 28 | Missing in CAA36682. Ref.2 | |||||||||||||||
| Sequence conflict | 29 | 1 | V → M in CAA36682. Ref.2 | |||||||||||||||
| Sequence conflict | 31 | 1 | P → S in AAA39844. Ref.4 | |||||||||||||||
| Sequence conflict | 31 | 1 | P → S in AAB19896. Ref.4 | |||||||||||||||
Secondary structure | ||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||
| Beta strand | 223 – 225 | 3 | ||||||||||||||||
| Helix | 232 – 242 | 11 | ||||||||||||||||
| Helix | 250 – 260 | 11 | ||||||||||||||||
| Helix | 264 – 275 | 12 | ||||||||||||||||
| Beta strand | 276 – 280 | 5 | ||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A POU-domain transcription factor in early stem cells and germ cells of the mammalian embryo." Rosner M.H., Vigano M.A., Ozato K., Timmons P.M., Poirier F., Rigby P.W.J., Staudt L. Nature 345:686-692(1990) [PubMed: 1972777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Embryonic carcinoma. |
| [2] | "New type of POU domain in germ line-specific protein Oct-4." Schoeler H.R., Ruppert S., Suzuki N., Chowdhury K., Gruss P. Nature 344:435-439(1990) [PubMed: 1690859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Tissue: Embryonic carcinoma. |
| [3] | "A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells." Okamoto K., Okazawa H., Okuda A., Sakai M., Muramatsu M., Hamada H. Cell 60:461-472(1990) [PubMed: 1967980] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [4] | "The oct3 gene, a gene for an embryonic transcription factor, is controlled by a retinoic acid repressible enhancer." Okazawa H., Okamoto K., Ishino F., Ishino-Kaneko T., Takeda S., Toyoda Y., Muramatsu M., Hamada H. EMBO J. 10:2997-3005(1991) [PubMed: 1915274] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION, INDUCTION. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo. |
| [6] | "Inhibition of DNA binding of Sox2 by the SUMO conjugation." Tsuruzoe S., Ishihara K., Uchimura Y., Watanabe S., Sekita Y., Aoto T., Saitoh H., Yuasa Y., Niwa H., Kawasuji M., Baba H., Nakao M. Biochem. Biophys. Res. Commun. 351:920-926(2006) [PubMed: 17097055] [Abstract] Cited for: SUMOYLATION AT LYS-118, MUTAGENESIS OF LYS-118. |
| [7] | "Post-translational modification of POU domain transcription factor Oct-4 by SUMO-1." Zhang Z., Liao B., Xu M., Jin Y. FASEB J. 21:3042-3051(2007) [PubMed: 17496161] [Abstract] Cited for: SUMOYLATION AT LYS-118, MUTAGENESIS OF LYS-118; GLU-120; LYS-215 AND LYS-244, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH UBE2I. |
| [8] | "Sumoylation of Oct4 enhances its stability, DNA binding, and transactivation." Wei F., Schoeler H.R., Atchison M.L. J. Biol. Chem. 282:21551-21560(2007) [PubMed: 17525163] [Abstract] Cited for: SUMOYLATION AT LYS-118, MUTAGENESIS OF LYS-118, FUNCTION, SUBCELLULAR LOCATION. |
| [9] | "Secondary structure of the oct-3 POU homeodomain as determined by 1H-15N NMR spectroscopy." Morita E.H., Shirakawa M., Hayashi F., Imagawa M., Kyogoku Y. FEBS Lett. 321:107-110(1993) [PubMed: 8097478] [Abstract] Cited for: STRUCTURE BY NMR OF POU DOMAIN. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M34381 mRNA. Translation: AAA39844.1. Sequence problems. X52437 mRNA. Translation: CAA36682.1. S58426 S58425 Genomic DNA. Translation: AAB19896.1. BC068268 mRNA. Translation: AAH68268.1. | |||||||||||||
| IPI | IPI00117218. | ||||||||||||
| PIR | A34672. S17313. | ||||||||||||
| RefSeq | NP_038661.2. | ||||||||||||
| UniGene | Mm.17031 | ||||||||||||
3D structure databases | |||||||||||||
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| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| IntAct | P20263. 5 interactions. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | P20263. | ||||||||||||
Proteomic databases | |||||||||||||
| PRIDE | P20263. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSMUST00000025271; ENSMUSP00000025271; ENSMUSG00000024406; Mus musculus. [Genome view] | ||||||||||||
| GeneID | 18999. | ||||||||||||
| KEGG | mmu:18999. | ||||||||||||
| UCSC | uc008chu.1. mouse. | ||||||||||||
Organism-specific databases | |||||||||||||
| CTD | 18999. | ||||||||||||
| MGI | MGI:101893. Pou5f1. | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOVERGEN | P20263. | ||||||||||||
| OMA | LENMFLQ. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | P20263. | ||||||||||||
| Bgee | P20263. | ||||||||||||
| CleanEx | MM_POU5F1. | ||||||||||||
| Genevestigator | P20263. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR012287. Homeodomain-rel. IPR013847. POU. IPR015585. POU_dom_5. IPR000327. POU_specific. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:1.10.10.60. Homeodomain-rel. 1 hit. | ||||||||||||
| PANTHER | PTHR11636:SF15. POU_5. 1 hit. | ||||||||||||
| Pfam | PF00046. Homeobox. 1 hit. PF00157. Pou. 1 hit. [Graphical view] | ||||||||||||
| PRINTS | PR00028. POUDOMAIN. | ||||||||||||
| ProDom | PD000010. Homeobox. 1 hit. PD000583. POU. 1 hit. [Graphical view] [Entries sharing at least one domain] | ||||||||||||
| SMART | SM00389. HOX. 1 hit. SM00352. POU. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. PS00035. POU_1. 1 hit. PS00465. POU_2. 1 hit. PS51179. POU_3. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| NextBio | 295410. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | PO5F1_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P20263 Secondary accession number(s): Q63843 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


