P20257 (PA2BE_PSEAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Basic phospholipase A2 PA-15 Short name=svPLA2 EC=3.1.1.4 Alternative name(s): Phosphatidylcholine 2-acylhydrolase
Organism	Pseudechis australis (Mulga snake) (King brown snake)
Taxonomic identifier	8670 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Acanthophiinae › Pseudechis

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion By similarity.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Toxic dose	LD50 is 3.4 mg/kg by intravenous injection. Ref.1
Sequence similarities	Belongs to the phospholipase A2 family. Group I subfamily. D49 sub-subfamily.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Secreted
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	Basic phospholipase A2 PA-15		PRO_0000161690
Sites
	Active site	48	1	By similarity
	Active site	92	1	By similarity
	Metal binding	28	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	30	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	32	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	49	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	11 ↔ 71		By similarity
	Disulfide bond	27 ↔ 117		By similarity
	Disulfide bond	29 ↔ 45		By similarity
	Disulfide bond	44 ↔ 98		By similarity
	Disulfide bond	51 ↔ 91		By similarity
	Disulfide bond	60 ↔ 84		By similarity
	Disulfide bond	78 ↔ 89		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20257 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: EA0FDFC13D91AC9A	FASTA	118	13,200
	10 20 30 40 50 60 NILQFRKMIQ CANKGSRAAW HYLDYGCYCG PGGRGTPVDE LDRCCKIHDD CYIEAGKDGC 70 80 90 100 110 YPKLTWYSWQ CTGDAPTCNP KSKCKDFVCA CDAAAAKCFA KAAYNKANWN IDTKTRCK

References

[1]	"Amino acid sequences of eight phospholipases A2 from the venom of Australian king brown snake, Pseudechis australis." Takasaki C., Yutani F., Kajiyashiki T. Toxicon 28:329-339(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, LETHAL DOSE. Tissue: Venom.

Cross-references

Sequence databases
PIR	H34860.
3D structure databases
ProteinModelPortal	P20257.
SMR	P20257. Positions 1-118.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008137.
Family and domain databases
Gene3D	1.20.90.10. 1 hit.
InterPro	IPR001211. PLipase_A2. IPR013090. PLipase_A2_AS. IPR016090. PLipase_A2_dom. [Graphical view]
PANTHER	PTHR11716. PTHR11716. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
SMART	SM00085. PA2c. 1 hit. [Graphical view]
SUPFAM	SSF48619. PhospholipaseA2. 1 hit.
PROSITE	PS00119. PA2_ASP. 1 hit. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA2BE_PSEAU

Accession

Primary (citable) accession number: P20257

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program