Reviewed, UniProtKB/Swiss-Prot P20254 (PA20_PSEAU)

Names and origin

Protein names	Recommended name:     Phospholipase A2 isozyme PA-10A     EC=3.1.1.4 Alternative name(s):     Phosphatidylcholine 2-acylhydrolase
Organism	Pseudechis australis (Mulga snake) (King brown snake)
Taxonomic identifier	8670 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Acanthophiinae › Pseudechis

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Miscellaneous	There are many protein components with phospholipase A2 activity in the Mulga snake venom and some of them are myotoxic.
Sequence similarities	Belongs to the phospholipase A2 family. Group I subfamily.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	Phospholipase A2 isozyme PA-10A		PRO_0000161685
Sites
	Active site	48	1	By similarity
	Active site	92	1	By similarity
	Metal binding	28	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	30	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	32	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	49	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	11 ↔ 71		By similarity
	Disulfide bond	27 ↔ 117		By similarity
	Disulfide bond	29 ↔ 45		By similarity
	Disulfide bond	44 ↔ 98		By similarity
	Disulfide bond	51 ↔ 91		By similarity
	Disulfide bond	60 ↔ 84		By similarity
	Disulfide bond	78 ↔ 89		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20254-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 884D1D3A6E2B5FCB	FASTA	118	13,027
	10 20 30 40 50 60 NLIQFSNMIQ CANKGSRPSL HYADYGCYCG WGGSGTPVDE LDRCCKVHDD CYDQAGKKGC 70 80 90 100 110 FPKLTLYSWD CTGNVPICNP KSKCKDFVCA CDAAAAKCFA KAPYNKANWN IDTKTRCK

References

[1]	"Amino acid sequences of eight phospholipases A2 from the venom of Australian king brown snake, Pseudechis australis." Takasaki C., Yutani F., Kajiyashiki T. Toxicon 28:329-339(1990) [PubMed: 2343466] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.

Cross-references

Sequence databases
PIR	E34860.
3D structure databases
SMR	P20254. Positions 1-118.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P20254.
Enzyme and pathway databases
BRENDA	3.1.1.4. 276288.
Family and domain databases
InterPro	IPR016090. Phospholipase_A2. IPR013090. Phospholipase_A2_AS. IPR001211. Phospholipase_A2_euk. [Graphical view]
Gene3D	G3DSA:1.20.90.10. Phospholipase_A2. 1 hit.
PANTHER	PTHR11716. Phospholipase_A2. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
SMART	SM00085. PA2c. 1 hit. [Graphical view]
PROSITE	PS00119. PA2_ASP. 1 hit. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA20_PSEAU

Accession

Primary (citable) accession number: P20254

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	November 24, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)