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P20249

- PA2A_GLOBL

UniProt

P20249 - PA2A_GLOBL

Protein

Acidic phospholipase A2

Gene
N/A
Organism
Gloydius blomhoffii (Mamushi) (Agkistrodon halys blomhoffi)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

    Catalytic activityi

    Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 calcium ion.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi27 – 271Calcium; via carbonyl oxygen
    Metal bindingi29 – 291Calcium; via carbonyl oxygen
    Metal bindingi30 – 301Calcium; via carbonyl oxygen
    Active sitei47 – 471
    Metal bindingi48 – 481Calcium
    Active sitei89 – 891

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phospholipase A2 activity Source: UniProtKB-EC

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW
    2. phospholipid metabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acidic phospholipase A2 (EC:3.1.1.4)
    Short name:
    PA2-II
    Short name:
    svPLA2
    Alternative name(s):
    Phosphatidylcholine 2-acylhydrolase
    OrganismiGloydius blomhoffii (Mamushi) (Agkistrodon halys blomhoffi)
    Taxonomic identifieri242054 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeGloydius

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 122122Acidic phospholipase A2PRO_0000161596Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi26 ↔ 115
    Disulfide bondi28 ↔ 44
    Disulfide bondi43 ↔ 95
    Disulfide bondi49 ↔ 122
    Disulfide bondi50 ↔ 88
    Disulfide bondi57 ↔ 81
    Disulfide bondi75 ↔ 86

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliP20249.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    HOVERGENiHBG008137.

    Family and domain databases

    Gene3Di1.20.90.10. 1 hit.
    InterProiIPR001211. PLipase_A2.
    IPR013090. PLipase_A2_AS.
    IPR016090. PLipase_A2_dom.
    [Graphical view]
    PANTHERiPTHR11716. PTHR11716. 1 hit.
    PfamiPF00068. Phospholip_A2_1. 1 hit.
    [Graphical view]
    PRINTSiPR00389. PHPHLIPASEA2.
    SMARTiSM00085. PA2c. 1 hit.
    [Graphical view]
    SUPFAMiSSF48619. SSF48619. 1 hit.
    PROSITEiPS00119. PA2_ASP. 1 hit.
    PS00118. PA2_HIS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20249-1 [UniParc]FASTAAdd to Basket

    « Hide

    SLMQFETLIM KIAGRSGIWY YGSYGCYCGA GGQGRPQDAS DRCCFVHDCC    50
    YGKVTGCDPK LDVYTYTEEN GAIVCGGDDP CKKQICECDK DAAICFRDNI 100
    DTYDNKYWFF PAKNCQEESE PC 122
    Length:122
    Mass (Da):13,664
    Last modified:February 1, 1991 - v1
    Checksum:i9728BC3E27CC5474
    GO

    Cross-referencesi

    3D structure databases

    ProteinModelPortali P20249.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG008137.

    Family and domain databases

    Gene3Di 1.20.90.10. 1 hit.
    InterProi IPR001211. PLipase_A2.
    IPR013090. PLipase_A2_AS.
    IPR016090. PLipase_A2_dom.
    [Graphical view ]
    PANTHERi PTHR11716. PTHR11716. 1 hit.
    Pfami PF00068. Phospholip_A2_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00389. PHPHLIPASEA2.
    SMARTi SM00085. PA2c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48619. SSF48619. 1 hit.
    PROSITEi PS00119. PA2_ASP. 1 hit.
    PS00118. PA2_HIS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Revised amino acid sequence, crystallization, and preliminary X-ray diffraction analysis of acidic phospholipase A2 from the venom of Agkistrodon halys blomhoffii."
      Tomoo K., Ohishi H., Ishida T., Inoue M., Ikeda K., Aoki Y., Samejima Y.
      J. Biol. Chem. 264:3636-3638(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
      Tissue: Venom.
    2. "Complete amino acid sequence of phospholipase A2-II isolated from Agkistrodon halys blomhoffii venom."
      Samejima Y., Iwanaga S., Suzuki T.
      FEBS Lett. 47:348-351(1974) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY PROTEIN SEQUENCE.
      Tissue: Venom.
    3. "Structure of acidic phospholipase A2 for the venom of Agkistrodon halys blomhoffii at 2.8-A resolution."
      Tomoo K., Ohishi H., Doi M., Ishida T., Inoue M., Ikeda K., Hata Y., Samejima Y.
      Biochem. Biophys. Res. Commun. 184:137-143(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).

    Entry informationi

    Entry nameiPA2A_GLOBL
    AccessioniPrimary (citable) accession number: P20249
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Is not neurotoxic.

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3