P20249 (PA2A_GLOBL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Acidic phospholipase A2 Short name=PA2-II Short name=svPLA2 EC=3.1.1.4 Alternative name(s): Phosphatidylcholine 2-acylhydrolase
Organism	Gloydius blomhoffii (Mamushi) (Agkistrodon halys blomhoffi)
Taxonomic identifier	242054 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Viperidae › Crotalinae › Gloydius

Protein attributes

Sequence length	122 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion.
Subunit structure	Monomer.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Miscellaneous	Is not neurotoxic.
Sequence similarities	Belongs to the phospholipase A2 family. Group II subfamily. D49 sub-subfamily.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Secreted
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 122	122	Acidic phospholipase A2		PRO_0000161596
Sites
	Active site	47	1
	Active site	89	1
	Metal binding	27	1	Calcium; via carbonyl oxygen
	Metal binding	29	1	Calcium; via carbonyl oxygen
	Metal binding	30	1	Calcium; via carbonyl oxygen
	Metal binding	48	1	Calcium
Amino acid modifications
	Disulfide bond	26 ↔ 115
	Disulfide bond	28 ↔ 44
	Disulfide bond	43 ↔ 95
	Disulfide bond	49 ↔ 122
	Disulfide bond	50 ↔ 88
	Disulfide bond	57 ↔ 81
	Disulfide bond	75 ↔ 86

Sequences

	Sequence		Length	Mass (Da)
	P20249 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 9728BC3E27CC5474	FASTA	122	13,664
	10 20 30 40 50 60 SLMQFETLIM KIAGRSGIWY YGSYGCYCGA GGQGRPQDAS DRCCFVHDCC YGKVTGCDPK 70 80 90 100 110 120 LDVYTYTEEN GAIVCGGDDP CKKQICECDK DAAICFRDNI DTYDNKYWFF PAKNCQEESE PC

References

[1]	"Revised amino acid sequence, crystallization, and preliminary X-ray diffraction analysis of acidic phospholipase A2 from the venom of Agkistrodon halys blomhoffii." Tomoo K., Ohishi H., Ishida T., Inoue M., Ikeda K., Aoki Y., Samejima Y. J. Biol. Chem. 264:3636-3638(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.
[2]	"Complete amino acid sequence of phospholipase A2-II isolated from Agkistrodon halys blomhoffii venom." Samejima Y., Iwanaga S., Suzuki T. FEBS Lett. 47:348-351(1974) [PubMed] [Europe PMC] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE. Tissue: Venom.
[3]	"Structure of acidic phospholipase A2 for the venom of Agkistrodon halys blomhoffii at 2.8-A resolution." Tomoo K., Ohishi H., Doi M., Ishida T., Inoue M., Ikeda K., Hata Y., Samejima Y. Biochem. Biophys. Res. Commun. 184:137-143(1992) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).

Cross-references

3D structure databases
ProteinModelPortal	P20249.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008137.
Family and domain databases
Gene3D	1.20.90.10. 1 hit.
InterPro	IPR001211. PLipase_A2. IPR013090. PLipase_A2_AS. IPR016090. PLipase_A2_dom. [Graphical view]
PANTHER	PTHR11716. PTHR11716. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
SMART	SM00085. PA2c. 1 hit. [Graphical view]
SUPFAM	SSF48619. PhospholipaseA2. 1 hit.
PROSITE	PS00119. PA2_ASP. 1 hit. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA2A_GLOBL

Accession

Primary (citable) accession number: P20249

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program