Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P20236

- GBRA3_RAT

UniProt

P20236 - GBRA3_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Gamma-aminobutyric acid receptor subunit alpha-3

Gene

Gabra3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

GO - Molecular functioni

  1. chloride channel activity Source: UniProtKB-KW
  2. extracellular ligand-gated ion channel activity Source: InterPro
  3. GABA-A receptor activity Source: RGD

GO - Biological processi

  1. gamma-aminobutyric acid signaling pathway Source: InterPro
  2. synaptic transmission Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit alpha-3
Alternative name(s):
GABA(A) receptor subunit alpha-3
Gene namesi
Name:Gabra3
Synonyms:Gabra-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2648. Gabra3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 276248ExtracellularCuratedAdd
BLAST
Transmembranei277 – 29822HelicalCuratedAdd
BLAST
Transmembranei304 – 32522HelicalCuratedAdd
BLAST
Transmembranei338 – 35922HelicalCuratedAdd
BLAST
Topological domaini360 – 45899CytoplasmicCuratedAdd
BLAST
Transmembranei459 – 48022HelicalCuratedAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. chloride channel complex Source: UniProtKB-KW
  3. postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 493465Gamma-aminobutyric acid receptor subunit alpha-3PRO_0000000439Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi191 ↔ 205By similarity
Glycosylationi228 – 2281N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP20236.

Expressioni

Gene expression databases

GenevestigatoriP20236.

Interactioni

Subunit structurei

Binds UBQLN1 (By similarity). Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
GphnQ03555-65EBI-5273284,EBI-5273276

Protein-protein interaction databases

IntActiP20236. 1 interaction.
MINTiMINT-8293904.
STRINGi10116.ENSRNOP00000050712.

Structurei

3D structure databases

ProteinModelPortaliP20236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG238757.
HOGENOMiHOG000231337.
HOVERGENiHBG051707.
InParanoidiP20236.
PhylomeDBiP20236.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR001390. GABAAa_rcpt.
IPR005433. GABBAa3_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 2 hits.
[Graphical view]
PRINTSiPR01079. GABAARALPHA.
PR01616. GABAARALPHA3.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20236-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MITTQMWHFY VTRVGLLLLI SILPGTTGQG ESRRQEPGDF VKQDIGGLSP
60 70 80 90 100
KHAPDIPDDS TDNITIFTRI LDRLLDGYDN RLRPGLGDAV TEVKTDIYVT
110 120 130 140 150
SFGPVSDTDM EYTIDVFFRQ TWHDERLKFD GPMKILPLNN LLASKIWTPD
160 170 180 190 200
TFFHNGKKSV AHNMTTPNKL LRLVDNGTLL YTMRLTIHAE CPMHLEDFPM
210 220 230 240 250
DVHACPLKFG SYAYTKAEVI YSWTLGKNKS VEVAQDGSRL NQYDLLGHVV
260 270 280 290 300
GTEIIRSSTG EYVVMTTHFH LKRKIGYFVI QTYLPCIMTV ILSQVSFWLN
310 320 330 340 350
RESVPARTVF GVTTVLTMTT LSISARNSLP KVAYATAMDW FMAVCYAFVF
360 370 380 390 400
SALIEFATVN YFTKRSWAWE GKKVPEALEM KKKTPAAPTK KTSTTFNIVG
410 420 430 440 450
TTYPINLALD TEFSTISKAA AAPSASSTPT VIASPKTTYV QDSPAETKTY
460 470 480 490
NSVSKVDKIS RIIFPVLFAI FNLVYWATYV NRESAIKGMI RKQ
Length:493
Mass (Da):55,433
Last modified:February 1, 1991 - v1
Checksum:i7145AA1A00D67B53
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti160 – 1601V → M in AAC42031. (PubMed:1966765)Curated
Sequence conflicti324 – 3241S → T in AAC42031. (PubMed:1966765)Curated
Sequence conflicti409 – 4091L → K in AAC42031. (PubMed:1966765)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08492 Genomic DNA. Translation: AAC42031.1.
X51991 mRNA. Translation: CAA36247.1.
PIRiA34130.
UniGeneiRn.10367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08492 Genomic DNA. Translation: AAC42031.1 .
X51991 mRNA. Translation: CAA36247.1 .
PIRi A34130.
UniGenei Rn.10367.

3D structure databases

ProteinModelPortali P20236.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P20236. 1 interaction.
MINTi MINT-8293904.
STRINGi 10116.ENSRNOP00000050712.

Chemistry

BindingDBi P20236.
ChEMBLi CHEMBL328.
GuidetoPHARMACOLOGYi 406.

Proteomic databases

PRIDEi P20236.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

RGDi 2648. Gabra3.

Phylogenomic databases

eggNOGi NOG238757.
HOGENOMi HOG000231337.
HOVERGENi HBG051707.
InParanoidi P20236.
PhylomeDBi P20236.

Miscellaneous databases

NextBioi 604953.

Gene expression databases

Genevestigatori P20236.

Family and domain databases

Gene3Di 2.70.170.10. 1 hit.
InterProi IPR006028. GABAA/Glycine_rcpt.
IPR001390. GABAAa_rcpt.
IPR005433. GABBAa3_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view ]
PANTHERi PTHR18945. PTHR18945. 1 hit.
Pfami PF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 2 hits.
[Graphical view ]
PRINTSi PR01079. GABAARALPHA.
PR01616. GABAARALPHA3.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMi SSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsi TIGR00860. LIC. 1 hit.
PROSITEi PS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Functional expression and sites of gene transcription of a novel alpha subunit of the GABAA receptor in rat brain."
    Malherbe P., Sigel E., Baur R., Persohn E., Richards J.G., Moehler H.
    FEBS Lett. 260:261-265(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "The GABAA receptor family: molecular and functional diversity."
    Seeburg P.H., Wisden W., Verdoorn T., Pritchett D., Werner P., Herb A., Lueddens H., Sprengel R., Sakmann B.
    Cold Spring Harb. Symp. Quant. Biol. 55:29-40(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Brain.

Entry informationi

Entry nameiGBRA3_RAT
AccessioniPrimary (citable) accession number: P20236
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: October 29, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3