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Protein

Gamma-aminobutyric acid receptor subunit alpha-3

Gene

Gabra3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

GO - Molecular functioni

GO - Biological processi

  • chemical synaptic transmission Source: RGD
  • gamma-aminobutyric acid signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit alpha-3
Alternative name(s):
GABA(A) receptor subunit alpha-3
Gene namesi
Name:Gabra3
Synonyms:Gabra-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2648. Gabra3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 276ExtracellularCuratedAdd BLAST248
Transmembranei277 – 298HelicalCuratedAdd BLAST22
Transmembranei304 – 325HelicalCuratedAdd BLAST22
Transmembranei338 – 359HelicalCuratedAdd BLAST22
Topological domaini360 – 458CytoplasmicCuratedAdd BLAST99
Transmembranei459 – 480HelicalCuratedAdd BLAST22

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL328.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000000043929 – 493Gamma-aminobutyric acid receptor subunit alpha-3Add BLAST465

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi63N-linked (GlcNAc...)Sequence analysis1
Glycosylationi163N-linked (GlcNAc...)Sequence analysis1
Glycosylationi176N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi191 ↔ 205By similarity
Glycosylationi228N-linked (GlcNAc...)Sequence analysis1
Modified residuei427PhosphoserineBy similarity1
Modified residuei428PhosphothreonineBy similarity1
Modified residuei434PhosphoserineBy similarity1
Modified residuei443PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP20236.
PRIDEiP20236.

PTM databases

iPTMnetiP20236.
PhosphoSitePlusiP20236.
UniCarbKBiP20236.

Interactioni

Subunit structurei

Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Binds UBQLN1 (By similarity). Interacts with GPHN (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
GphnQ03555-65EBI-5273284,EBI-5273276

Protein-protein interaction databases

IntActiP20236. 1 interactor.
MINTiMINT-8293904.
STRINGi10116.ENSRNOP00000050712.

Chemistry databases

BindingDBiP20236.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4TK1X-ray2.70C/D396-406[»]
4TK2X-ray4.10C/D396-406[»]
4TK3X-ray2.70C/D396-406[»]
4TK4X-ray3.60C/D396-406[»]
4U90X-ray2.00D/E396-404[»]
ProteinModelPortaliP20236.
SMRiP20236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3642. Eukaryota.
ENOG410XNQG. LUCA.
HOGENOMiHOG000231337.
HOVERGENiHBG051707.
InParanoidiP20236.
PhylomeDBiP20236.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR001390. GABAAa_rcpt.
IPR005433. GABBAa3_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01079. GABAARALPHA.
PR01616. GABAARALPHA3.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20236-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITTQMWHFY VTRVGLLLLI SILPGTTGQG ESRRQEPGDF VKQDIGGLSP
60 70 80 90 100
KHAPDIPDDS TDNITIFTRI LDRLLDGYDN RLRPGLGDAV TEVKTDIYVT
110 120 130 140 150
SFGPVSDTDM EYTIDVFFRQ TWHDERLKFD GPMKILPLNN LLASKIWTPD
160 170 180 190 200
TFFHNGKKSV AHNMTTPNKL LRLVDNGTLL YTMRLTIHAE CPMHLEDFPM
210 220 230 240 250
DVHACPLKFG SYAYTKAEVI YSWTLGKNKS VEVAQDGSRL NQYDLLGHVV
260 270 280 290 300
GTEIIRSSTG EYVVMTTHFH LKRKIGYFVI QTYLPCIMTV ILSQVSFWLN
310 320 330 340 350
RESVPARTVF GVTTVLTMTT LSISARNSLP KVAYATAMDW FMAVCYAFVF
360 370 380 390 400
SALIEFATVN YFTKRSWAWE GKKVPEALEM KKKTPAAPTK KTSTTFNIVG
410 420 430 440 450
TTYPINLALD TEFSTISKAA AAPSASSTPT VIASPKTTYV QDSPAETKTY
460 470 480 490
NSVSKVDKIS RIIFPVLFAI FNLVYWATYV NRESAIKGMI RKQ
Length:493
Mass (Da):55,433
Last modified:February 1, 1991 - v1
Checksum:i7145AA1A00D67B53
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti160V → M in AAC42031 (PubMed:1966765).Curated1
Sequence conflicti324S → T in AAC42031 (PubMed:1966765).Curated1
Sequence conflicti409L → K in AAC42031 (PubMed:1966765).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08492 Genomic DNA. Translation: AAC42031.1.
X51991 mRNA. Translation: CAA36247.1.
PIRiA34130.
UniGeneiRn.10367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08492 Genomic DNA. Translation: AAC42031.1.
X51991 mRNA. Translation: CAA36247.1.
PIRiA34130.
UniGeneiRn.10367.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4TK1X-ray2.70C/D396-406[»]
4TK2X-ray4.10C/D396-406[»]
4TK3X-ray2.70C/D396-406[»]
4TK4X-ray3.60C/D396-406[»]
4U90X-ray2.00D/E396-404[»]
ProteinModelPortaliP20236.
SMRiP20236.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP20236. 1 interactor.
MINTiMINT-8293904.
STRINGi10116.ENSRNOP00000050712.

Chemistry databases

BindingDBiP20236.
ChEMBLiCHEMBL328.

PTM databases

iPTMnetiP20236.
PhosphoSitePlusiP20236.
UniCarbKBiP20236.

Proteomic databases

PaxDbiP20236.
PRIDEiP20236.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi2648. Gabra3.

Phylogenomic databases

eggNOGiKOG3642. Eukaryota.
ENOG410XNQG. LUCA.
HOGENOMiHOG000231337.
HOVERGENiHBG051707.
InParanoidiP20236.
PhylomeDBiP20236.

Miscellaneous databases

PROiP20236.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR001390. GABAAa_rcpt.
IPR005433. GABBAa3_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01079. GABAARALPHA.
PR01616. GABAARALPHA3.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGBRA3_RAT
AccessioniPrimary (citable) accession number: P20236
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 2, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.