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Protein

Glutamate decarboxylase

Gene

Gad1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the production of GABA.

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei483SubstrateBy similarity1

GO - Molecular functioni

  • glutamate decarboxylase activity Source: FlyBase
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • gamma-aminobutyric acid biosynthetic process Source: FlyBase
  • glutamate catabolic process Source: FlyBase
  • larval locomotory behavior Source: FlyBase
  • neuromuscular junction development Source: FlyBase
  • neurotransmitter biosynthetic process Source: UniProtKB-KW
  • neurotransmitter receptor metabolic process Source: FlyBase
  • olfactory learning Source: FlyBase
  • response to mechanical stimulus Source: FlyBase
  • synapse assembly Source: FlyBase

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processNeurotransmitter biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BRENDAi4.1.1.15. 1994.
ReactomeiR-DME-888568. GABA synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase (EC:4.1.1.15)
Short name:
GAD
Gene namesi
Name:Gad1
Synonyms:Gad, Glb
ORF Names:CG14994
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0004516. Gad1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001469721 – 510Glutamate decarboxylaseAdd BLAST510

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei322N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiP20228.
PRIDEiP20228.

Expressioni

Gene expression databases

BgeeiFBgn0004516.
ExpressionAtlasiP20228. differential.
GenevisibleiP20228. DM.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi63964. 4 interactors.
IntActiP20228. 1 interactor.
MINTiMINT-917310.
STRINGi7227.FBpp0073131.

Structurei

3D structure databases

ProteinModelPortaliP20228.
SMRiP20228.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 109Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the group II decarboxylase family.Curated

Phylogenomic databases

eggNOGiKOG0629. Eukaryota.
COG0076. LUCA.
GeneTreeiENSGT00760000119205.
InParanoidiP20228.
KOiK01580.
OMAiTVNPHKM.
OrthoDBiEOG091G07ZU.
PhylomeDBiP20228.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiView protein in InterPro
IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_sub2.
IPR021115. Pyridoxal-P_BS.
PfamiView protein in Pfam
PF00282. Pyridoxal_deC. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiView protein in PROSITE
PS00392. DDC_GAD_HDC_YDC. 1 hit.

Sequencei

Sequence statusi: Complete.

P20228-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLNPNGYKL SERTGKLTAY DLMPTTVTAG PETREFLLKV IDVLLDFVKA
60 70 80 90 100
TNDRNEKVLD FHHPEDMKRL LDLDVPDRAL PLQQLIEDCA TTLKYQVKTG
110 120 130 140 150
HPHFFNQLSN GLDLISMAGE WLTATANTNM FTYEIAPVFI LMENVVLTKM
160 170 180 190 200
REIIGWSGGD SILAPGGSIS NLYAFLAARH KMFPNYKEHG SVGLPGTLVM
210 220 230 240 250
FTSDQCHYSI KSCAAVCGLG TDHCIVVPSD EHGKMITSEL ERLILERKAK
260 270 280 290 300
GDIPFFVNAT AGTTVLGAFD DINTIADICQ KYNCWMHIDA AWGGGLLMSR
310 320 330 340 350
KHRHPRFTGV ERADSVTWNP HKLMGALLQC STIHFKEDGL LISCNQMSAE
360 370 380 390 400
YLFMTDKQYD ISYDTGDKVI QCGRHNDIFK LWLQWRAKGT EGFEQQQDRL
410 420 430 440 450
MELVQYQLKR IREQSDRFHL ILEPECVNVS FWYVPKRLRG VPHDAKKEVE
460 470 480 490 500
LGKICPIIKG RMMQKGTLMV GYQPDDRRPN FFRSIISSAA VNEADVDFML
510
DEIHRLGDDL
Length:510
Mass (Da):57,819
Last modified:June 21, 2005 - v2
Checksum:iAAEBFC6FDC662001
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti201F → L in CAA53791 (PubMed:1689376).Curated1
Sequence conflicti301K → T in CAA53791 (PubMed:1689376).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76198 mRNA. Translation: CAA53791.1.
AE014296 Genomic DNA. Translation: AAF47834.1.
AE014296 Genomic DNA. Translation: AAN11581.1.
AE014296 Genomic DNA. Translation: AAN11582.1.
AY089526 mRNA. Translation: AAL90264.1.
PIRiJH0192. A30999.
RefSeqiNP_001261396.1. NM_001274467.1.
NP_523914.2. NM_079190.3.
NP_728930.1. NM_168055.2.
NP_728931.1. NM_168056.2.
UniGeneiDm.4963.

Genome annotation databases

EnsemblMetazoaiFBtr0073275; FBpp0073131; FBgn0004516.
FBtr0073276; FBpp0073132; FBgn0004516.
FBtr0073277; FBpp0073133; FBgn0004516.
FBtr0332980; FBpp0305196; FBgn0004516.
GeneIDi38484.
KEGGidme:Dmel_CG14994.

Similar proteinsi

Entry informationi

Entry nameiDCE_DROME
AccessioniPrimary (citable) accession number: P20228
Secondary accession number(s): A4V1G1, Q9VZI7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: June 21, 2005
Last modified: October 25, 2017
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families