P20228 (DCE_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate decarboxylase
Short name=GAD
EC=4.1.1.15

Gene names

Name:	Gad1
Synonyms:	Gad, Glb
ORF Names:	CG14994

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the production of GABA.
Catalytic activity	L-glutamate = 4-aminobutanoate + CO2.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the group II decarboxylase family.

Ontologies

Keywords
Biological process	Neurotransmitter biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	gamma-aminobutyric acid biosynthetic process Inferred from mutant phenotype PubMed 12603819. Source: FlyBase glutamate catabolic process Inferred from mutant phenotype PubMed 11753421. Source: FlyBase larval locomotory behavior Inferred from mutant phenotype PubMed 10939332. Source: FlyBase neuromuscular junction development Inferred from mutant phenotype PubMed 10939332. Source: FlyBase neurotransmitter biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW neurotransmitter receptor metabolic process Inferred from mutant phenotype PubMed 11753421. Source: FlyBase olfactory learning Inferred from mutant phenotype PubMed 19043409. Source: FlyBase response to mechanical stimulus Inferred from genetic interaction PubMed 16943550. Source: FlyBase synapse assembly Inferred from mutant phenotype PubMed 11753421. Source: FlyBase
Molecular_function	glutamate decarboxylase activity Inferred from direct assay PubMed 10939332. Source: FlyBase pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Glutamate decarboxylase		PRO_0000146972
Regions
	Region	107 – 109	3	Substrate binding By similarity
Sites
	Binding site	483	1	Substrate By similarity
Amino acid modifications
	Modified residue	322	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	201	1	F → L in CAA53791. Ref.1
	Sequence conflict	301	1	K → T in CAA53791. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P20228 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: AAEBFC6FDC662001	FASTA	510	57,819
	10 20 30 40 50 60 MSLNPNGYKL SERTGKLTAY DLMPTTVTAG PETREFLLKV IDVLLDFVKA TNDRNEKVLD 70 80 90 100 110 120 FHHPEDMKRL LDLDVPDRAL PLQQLIEDCA TTLKYQVKTG HPHFFNQLSN GLDLISMAGE 130 140 150 160 170 180 WLTATANTNM FTYEIAPVFI LMENVVLTKM REIIGWSGGD SILAPGGSIS NLYAFLAARH 190 200 210 220 230 240 KMFPNYKEHG SVGLPGTLVM FTSDQCHYSI KSCAAVCGLG TDHCIVVPSD EHGKMITSEL 250 260 270 280 290 300 ERLILERKAK GDIPFFVNAT AGTTVLGAFD DINTIADICQ KYNCWMHIDA AWGGGLLMSR 310 320 330 340 350 360 KHRHPRFTGV ERADSVTWNP HKLMGALLQC STIHFKEDGL LISCNQMSAE YLFMTDKQYD 370 380 390 400 410 420 ISYDTGDKVI QCGRHNDIFK LWLQWRAKGT EGFEQQQDRL MELVQYQLKR IREQSDRFHL 430 440 450 460 470 480 ILEPECVNVS FWYVPKRLRG VPHDAKKEVE LGKICPIIKG RMMQKGTLMV GYQPDDRRPN 490 500 510 FFRSIISSAA VNEADVDFML DEIHRLGDDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Drosophila GABAergic systems: sequence and expression of glutamic acid decarboxylase." Jackson F.R., Newby L.M., Kulkarni S.J. J. Neurochem. 54:1068-1078(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76198 mRNA. Translation: CAA53791.1. AE014296 Genomic DNA. Translation: AAF47834.1. AE014296 Genomic DNA. Translation: AAN11581.1. AE014296 Genomic DNA. Translation: AAN11582.1. AY089526 mRNA. Translation: AAL90264.1.
PIR	A30999. JH0192.
RefSeq	NP_001261396.1. NM_001274467.1. NP_523914.2. NM_079190.3. NP_728930.1. NM_168055.2. NP_728931.1. NM_168056.2.
UniGene	Dm.4963.
3D structure databases
ProteinModelPortal	P20228.
SMR	P20228. Positions 17-510.
ModBase	Search...
Protein-protein interaction databases
IntAct	P20228. 1 interaction.
MINT	MINT-917310.
STRING	7227.FBpp0073133.
Proteomic databases
PaxDb	P20228.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0073275; FBpp0073131; FBgn0004516. FBtr0073276; FBpp0073132; FBgn0004516. FBtr0073277; FBpp0073133; FBgn0004516. FBtr0332980; FBpp0305196; FBgn0004516.
GeneID	38484.
KEGG	dme:Dmel_CG14994.
Organism-specific databases
CTD	2571.
FlyBase	FBgn0004516. Gad1.
Phylogenomic databases
eggNOG	COG0076.
GeneTree	ENSGT00550000074275.
InParanoid	P20228.
KO	K01580.
OMA	ISMAGEW.
OrthoDB	EOG4V9S5R.
PhylomeDB	P20228.
Gene expression databases
Bgee	P20228.
GermOnline	CG14994. Drosophila melanogaster.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR002129. PyrdxlP-dep_de-COase. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. IPR021115. Pyridoxal-P_BS. [Graphical view]
Pfam	PF00282. Pyridoxal_deC. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00392. DDC_GAD_HDC_YDC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	38484.
NextBio	808870.

Entry information

Entry name

DCE_DROME

Accession

Primary (citable) accession number: P20228
Secondary accession number(s): A4V1G1, Q9VZI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	June 21, 2005
Last modified:	May 1, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project