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P20192

- DGKA_PIG

UniProt

P20192 - DGKA_PIG

Protein

Diacylglycerol kinase alpha

Gene

DGKA

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Enzyme regulationi

    Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi122 – 133121CuratedAdd
    BLAST
    Calcium bindingi167 – 178122CuratedAdd
    BLAST
    Zinc fingeri204 – 25249Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri268 – 31851Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium ion binding Source: InterPro
    3. diacylglycerol kinase activity Source: UniProtKB-EC
    4. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    SABIO-RKP20192.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase alpha (EC:2.7.1.107)
    Short name:
    DAG kinase alpha
    Alternative name(s):
    80 kDa diacylglycerol kinase
    Diglyceride kinase alpha
    Short name:
    DGK-alpha
    Gene namesi
    Name:DGKA
    Synonyms:DAGK, DAGK1
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Chromosome 5

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi248 – 2481K → R: No decrease in activity. 1 Publication
    Mutagenesisi383 – 3831K → N: No decrease in activity. 1 Publication
    Mutagenesisi395 – 3951K → N: No decrease in activity. 1 Publication
    Mutagenesisi483 – 4831K → N: No decrease in activity. 1 Publication
    Mutagenesisi492 – 4921K → R: No decrease in activity. 1 Publication
    Mutagenesisi554 – 5541K → N: No decrease in activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 734734Diacylglycerol kinase alphaPRO_0000218455Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei483 – 4831N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Expressioni

    Tissue specificityi

    Lymphocytes and oligodendroglial cells.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi9823.ENSSSCP00000000387.

    Structurei

    3D structure databases

    ProteinModelPortaliP20192.
    SMRiP20192. Positions 1-117.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini109 – 14436EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini154 – 18936EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini371 – 505135DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri204 – 25249Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri268 – 31851Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47311.
    GeneTreeiENSGT00750000117569.
    HOGENOMiHOG000252931.
    HOVERGENiHBG051345.
    KOiK00901.
    OMAiLVGLHCV.
    OrthoDBiEOG75XGK8.
    TreeFamiTF313104.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    1.10.238.110. 1 hit.
    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR029477. DAG_kinase_typeI_N.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF00130. C1_1. 2 hits.
    PF14513. DAG_kinase_N. 2 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 2 hits.
    PROSITEiPS50146. DAGK. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20192-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKERGLISP SDFAQLQKYM EYSTKKVSDV LKLFEDGEMA EYLQGDAIGY    50
    EGFQQFLKIY LEVDSVPSHL SLALFQSFQT SYCSEETVKR DVVCLSDVSC 100
    YFSLLEGGRP EDKLEFTFKL YDTDRNGILD SSEVDRIIIQ MMRMAEYLDW 150
    DVSELRPILQ EMMKEIDYDG SGSVSLAEWL RAGATTVPLL VLLGLEMTLK 200
    DNGQHMWRPK RFPRPVYCNL CESSIGLGKQ GLSCNLCKYT VHDQCAMKAL 250
    PCEVSTYAKS RKDIGVQTHV WVRGGCESGR CDRCQKKIRI YHSLVGLHCV 300
    WCHLEIHDDC LPAMGHECDC GLLRDHILPP SSIYPSVLAS GQERKVSKTS 350
    QKTTDDLNLS TSEALRIDPV SNTHPLLVFV NPKSGGKQGE RVLWKFQYLL 400
    NPRQVFNLLK DGPEPGLRFF REVPDYRILV CGGDGTVGWI LETIDKANLP 450
    FVPPVAVLPL GTGNDLARCL RWGGGYEGQN LGKILKDLEA SKVVHMDRWS 500
    VEVIPQQTEE KSDPVPFQII NNYFSIGVDA SIAHRFHIMR EKYPEKFNSR 550
    MKNKLWYFEF ATSESIFSTC KKLEESLTVE ICGKPLDLSN LSLEGIAVLN 600
    IPSTHGGSNL WGDTKRPHGD IHGINQALGA MAKVITDPDI LKTCVPDLSD 650
    KRLEVVGLEG AIEMGQIYTK LKNAGHRLAK CSEITFHTTK TLPMQIDGEP 700
    WMQTPCTIKI THRNQMPMLV GPPPRSSNFF GFLC 734
    Length:734
    Mass (Da):82,606
    Last modified:February 1, 1991 - v1
    Checksum:i711C2E66FB4B4E80
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53256 mRNA. Translation: CAA37347.1.
    PIRiS09156.
    RefSeqiNP_999197.1. NM_214032.2.
    XP_005663934.1. XM_005663877.1.
    UniGeneiSsc.54911.

    Genome annotation databases

    EnsembliENSSSCT00000000393; ENSSSCP00000000387; ENSSSCG00000000370.
    GeneIDi397097.
    KEGGissc:397097.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53256 mRNA. Translation: CAA37347.1 .
    PIRi S09156.
    RefSeqi NP_999197.1. NM_214032.2.
    XP_005663934.1. XM_005663877.1.
    UniGenei Ssc.54911.

    3D structure databases

    ProteinModelPortali P20192.
    SMRi P20192. Positions 1-117.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9823.ENSSSCP00000000387.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSSSCT00000000393 ; ENSSSCP00000000387 ; ENSSSCG00000000370 .
    GeneIDi 397097.
    KEGGi ssc:397097.

    Organism-specific databases

    CTDi 1606.

    Phylogenomic databases

    eggNOGi NOG47311.
    GeneTreei ENSGT00750000117569.
    HOGENOMi HOG000252931.
    HOVERGENi HBG051345.
    KOi K00901.
    OMAi LVGLHCV.
    OrthoDBi EOG75XGK8.
    TreeFami TF313104.

    Enzyme and pathway databases

    SABIO-RK P20192.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    1.10.238.110. 1 hit.
    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR029477. DAG_kinase_typeI_N.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF00130. C1_1. 2 hits.
    PF14513. DAG_kinase_N. 2 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 2 hits.
    PROSITEi PS50146. DAGK. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Porcine diacylglycerol kinase sequence has zinc finger and E-F hand motifs."
      Sakane F., Yamada K., Kanoh H., Yokoyama C., Tanabe T.
      Nature 344:345-348(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Lymphocyte.
    2. "The C-terminal part of diacylglycerol kinase alpha lacking zinc fingers serves as a catalytic domain."
      Sakane F., Kai M., Wada I., Imai S., Kanoh H.
      Biochem. J. 318:583-590(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN CATALYTIC, MUTAGENESIS.

    Entry informationi

    Entry nameiDGKA_PIG
    AccessioniPrimary (citable) accession number: P20192
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3