Reviewed, UniProtKB/Swiss-Prot P20192 (DGKA_PIG)

Names and origin

Protein names

Recommended name:
    Diacylglycerol kinase alpha
      Short name=DAG kinase alpha
    EC=2.7.1.107
Alternative name(s):
    Diglyceride kinase alpha
      Short name=DGK-alpha
    80 kDa diacylglycerol kinase

Gene names

Name:	DGKA
Synonyms:	DAGK, DAGK1

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	734 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Enzyme regulation	Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C.
Subunit structure	Monomer.
Tissue specificity	Lymphocytes and oligodendroglial cells.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 2 EF-hand domains. Contains 2 phorbol-ester/DAG-type zinc fingers.

Ontologies

Keywords
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Calcium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: InterPro intracellular signaling cascade Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 734	734	Diacylglycerol kinase alpha		PRO_0000218455
Regions
	Domain	109 – 144	36	EF-hand 1
	Domain	154 – 189	36	EF-hand 2
	Domain	371 – 505	135	DAGKc
	Calcium binding	122 – 133	12	1 Probable
	Calcium binding	167 – 178	12	2 Probable
	Zinc finger	204 – 252	49	Phorbol-ester/DAG-type 1
	Zinc finger	268 – 318	51	Phorbol-ester/DAG-type 2
Amino acid modifications
	Modified residue	22	1	Phosphotyrosine By similarity
	Modified residue	483	1	N6-acetyllysine By similarity
Experimental info
	Mutagenesis	248	1	K → R: No decrease in activity.
	Mutagenesis	383	1	K → N: No decrease in activity.
	Mutagenesis	395	1	K → N: No decrease in activity.
	Mutagenesis	483	1	K → N: No decrease in activity.
	Mutagenesis	492	1	K → R: No decrease in activity.
	Mutagenesis	554	1	K → N: No decrease in activity.

Sequences

	Sequence		Length	Mass (Da)
	P20192-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 711C2E66FB4B4E80	FASTA	734	82,606
	10 20 30 40 50 60 MSKERGLISP SDFAQLQKYM EYSTKKVSDV LKLFEDGEMA EYLQGDAIGY EGFQQFLKIY 70 80 90 100 110 120 LEVDSVPSHL SLALFQSFQT SYCSEETVKR DVVCLSDVSC YFSLLEGGRP EDKLEFTFKL 130 140 150 160 170 180 YDTDRNGILD SSEVDRIIIQ MMRMAEYLDW DVSELRPILQ EMMKEIDYDG SGSVSLAEWL 190 200 210 220 230 240 RAGATTVPLL VLLGLEMTLK DNGQHMWRPK RFPRPVYCNL CESSIGLGKQ GLSCNLCKYT 250 260 270 280 290 300 VHDQCAMKAL PCEVSTYAKS RKDIGVQTHV WVRGGCESGR CDRCQKKIRI YHSLVGLHCV 310 320 330 340 350 360 WCHLEIHDDC LPAMGHECDC GLLRDHILPP SSIYPSVLAS GQERKVSKTS QKTTDDLNLS 370 380 390 400 410 420 TSEALRIDPV SNTHPLLVFV NPKSGGKQGE RVLWKFQYLL NPRQVFNLLK DGPEPGLRFF 430 440 450 460 470 480 REVPDYRILV CGGDGTVGWI LETIDKANLP FVPPVAVLPL GTGNDLARCL RWGGGYEGQN 490 500 510 520 530 540 LGKILKDLEA SKVVHMDRWS VEVIPQQTEE KSDPVPFQII NNYFSIGVDA SIAHRFHIMR 550 560 570 580 590 600 EKYPEKFNSR MKNKLWYFEF ATSESIFSTC KKLEESLTVE ICGKPLDLSN LSLEGIAVLN 610 620 630 640 650 660 IPSTHGGSNL WGDTKRPHGD IHGINQALGA MAKVITDPDI LKTCVPDLSD KRLEVVGLEG 670 680 690 700 710 720 AIEMGQIYTK LKNAGHRLAK CSEITFHTTK TLPMQIDGEP WMQTPCTIKI THRNQMPMLV 730 GPPPRSSNFF GFLC

References

[1]	"Porcine diacylglycerol kinase sequence has zinc finger and E-F hand motifs." Sakane F., Yamada K., Kanoh H., Yokoyama C., Tanabe T. Nature 344:345-348(1990) [PubMed: 2156169] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Lymphocyte.
[2]	"The C-terminal part of diacylglycerol kinase alpha lacking zinc fingers serves as a catalytic domain." Sakane F., Kai M., Wada I., Imai S., Kanoh H. Biochem. J. 318:583-590(1996) [PubMed: 8809050] [Abstract] Cited for: DOMAIN CATALYTIC, MUTAGENESIS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X53256 mRNA. Translation: CAA37347.1.
PIR	S09156.
RefSeq	NP_999197.1.
UniGene	Ssc.54911
3D structure databases
HSSP	HSSP built from PDB template 1M39 based on UniProtKB P41208.
SMR	P20192. Positions 1-117.
ModBase	Search...
Genome annotation databases
GeneID	397097.
KEGG	ssc:397097.
Organism-specific databases
CTD	397097.
Phylogenomic databases
HOVERGEN	P20192.
Enzyme and pathway databases
BRENDA	2.7.1.107. 249.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kinase_access. IPR001206. Diacylglycerol_kinase_cat. IPR018248. EF_hand. IPR018247. EF_HAND_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. [Graphical view]
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. PF00036. efhand. 1 hit. [Graphical view]
ProDom	PD002939. DAGKa. 1 hit. PD005043. DAGKc. 1 hit. PD000012. EF-hand. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGKA_PIG

Accession

Primary (citable) accession number: P20192

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	October 13, 2009
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)