P20179 (RDRP_CCMV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase 2a
Short name=protein 2a
EC=2.7.7.48

Gene names

ORF Names:

ORF2a

Organism

Cowpea chlorotic mottle virus (CCMV) [Complete proteome]

Taxonomic identifier

12303 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Bromoviridae › Bromovirus

Virus host

Glycine max (Soybean) (Glycine hispida) [TaxID: 3847]
Vigna unguiculata (Cowpea) [TaxID: 3917]

Protein attributes

Sequence length	808 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which replicates the viral genome composed of 3 RNA segments, RNA1, RNA2 and RNA3 Potential.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Interacts with replication protein 1a By similarity.
Sequence similarities	Belongs to the ssRNA positive-strand viruses RNA-directed RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 808	808	RNA-directed RNA polymerase 2a		PRO_0000083270
Regions
	Domain	470 – 583	114	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	P20179 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: D39AFFE56D29B2BE	FASTA	808	92,937
	10 20 30 40 50 60 MSKFIPEGET YHVPSFQWMF DQTLESDSHH DEAIFVTESI NESGVDTSVE ITADGTLASY 70 80 90 100 110 120 MHAVKPLVED GLLNPPFDQA RWGLCCKNVV DVYDGLLGYR LIPMAEAARM LYLEIDGSFV 130 140 150 160 170 180 DESECDDWRP VDTSDGFTEA MFDVMNEIPG EETKNTCALS LEAESRQAPE TSDMVPSEYT 190 200 210 220 230 240 LADRYVTTRE EFASVDSDYD ISLNLVSPVE FRVGVCEDTY RHSEADDPTM PQYHDRISLK 250 260 270 280 290 300 SLEAAGHHML PTHAYFDDTY YQALEELGDY NVDISKLSVR QSDVDWYRDP EKYYEPELSI 310 320 330 340 350 360 GSFQRRIGTQ KTVLTALKKR NADVPELADS VDIKRVACEV AEKFKRAYLN HSGIGLLGQS 370 380 390 400 410 420 MDVMSRGLEY HKKWKDHKDL TGVTVLSEIN LQRYQHMIKS DIKPVVSDTL HLERAVAATI 430 440 450 460 470 480 TFHGKGVTSC FSPYFTACFE KFSKALKSRF VVPIGKISSL ELKNVPLSNK WFLEADLSKF 490 500 510 520 530 540 DKSQGELHLE FQREILLSLG FPAPLTNWWC DFHRESMLSD PHAGVNMPVS FQRRTGDAFT 550 560 570 580 590 600 YFGNTLVTMA MMAYCCDMNT VDCAIFSGDD SLLICKSKPH LDANVFQSLF NMEIKVMDPS 610 620 630 640 650 660 LPYVCSKFLL ETEMNNLVSV PDPMREIQRL AKRKIIKSPE LLRAHFESFC DRMKFLNKLD 670 680 690 700 710 720 EKMINLLCKF VALKYKKPDV ENDVRVAIAA FGYYSENFLR FCECYATEGV NIYKVKHPIT 730 740 750 760 770 780 QEWFEASRDR DGDWFHDWRN PKFPTALDKV WRFFGKYARD DPMKHIEERD RRHRLNRAMN 790 800 SSLKLAYDRR SLSKDKETVA WVRKTLSK

References

[1]	"Sequence of cowpea chlorotic mottle virus RNAs 2 and 3 and evidence of a recombination event during bromovirus evolution." Allison R.F., Janda M., Ahlquist P. Virology 172:321-330(1989) [PubMed: 2773323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M28817 Genomic RNA. Translation: AAA46371.1.
PIR	P2WMCC. JU0117.
RefSeq	NP_613275.1. NC_003541.1.
3D structure databases
ProteinModelPortal	P20179.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	962150.
Family and domain databases
InterPro	IPR007610. BBMV_Gp1_N. IPR007094. RNA-dir_pol_PSvirus. IPR001788. Tymovirus_RNA-dep_RNA_pol. [Graphical view]
Pfam	PF04522. DUF585. 1 hit. PF00978. RdRP_2. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_CCMV

Accession

Primary (citable) accession number: P20179

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	October 19, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program