Reviewed, UniProtKB/Swiss-Prot P20179 (ORF2A_CCMV)

Names and origin

Protein names

Recommended name:
    RNA-directed RNA polymerase 2A
      Short name=protein 2A
    EC=2.7.7.48

Gene names

Name:	RNA2
ORF Names:	ORF2a

Organism

Cowpea chlorotic mottle virus (CCMV)

Taxonomic identifier

12303 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Bromoviridae › Bromovirus

Virus host

Glycine max (Soybean) [TaxID: 3847]
Vigna unguiculata (Cowpea) [TaxID: 3917]

Protein attributes

Sequence length	808 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNA-directed RNA polymerase.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Miscellaneous	The virus has a tripartite genome (RNA1, RNA2 and RNA3) and an encapsidated subgenomic RNA (RNA4) from which the coat protein is expressed. Virions are icosahedral, not enveloped, with 32 capsomers per nucleocapsid.
Sequence similarities	Belongs to the cucumoviruses/bromoviruses 2A family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 808	808	RNA-directed RNA polymerase 2A		PRO_0000083270
Regions
	Domain	470 – 583	114	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	P20179-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: D39AFFE56D29B2BE	FASTA	808	92,937
	10 20 30 40 50 60 MSKFIPEGET YHVPSFQWMF DQTLESDSHH DEAIFVTESI NESGVDTSVE ITADGTLASY 70 80 90 100 110 120 MHAVKPLVED GLLNPPFDQA RWGLCCKNVV DVYDGLLGYR LIPMAEAARM LYLEIDGSFV 130 140 150 160 170 180 DESECDDWRP VDTSDGFTEA MFDVMNEIPG EETKNTCALS LEAESRQAPE TSDMVPSEYT 190 200 210 220 230 240 LADRYVTTRE EFASVDSDYD ISLNLVSPVE FRVGVCEDTY RHSEADDPTM PQYHDRISLK 250 260 270 280 290 300 SLEAAGHHML PTHAYFDDTY YQALEELGDY NVDISKLSVR QSDVDWYRDP EKYYEPELSI 310 320 330 340 350 360 GSFQRRIGTQ KTVLTALKKR NADVPELADS VDIKRVACEV AEKFKRAYLN HSGIGLLGQS 370 380 390 400 410 420 MDVMSRGLEY HKKWKDHKDL TGVTVLSEIN LQRYQHMIKS DIKPVVSDTL HLERAVAATI 430 440 450 460 470 480 TFHGKGVTSC FSPYFTACFE KFSKALKSRF VVPIGKISSL ELKNVPLSNK WFLEADLSKF 490 500 510 520 530 540 DKSQGELHLE FQREILLSLG FPAPLTNWWC DFHRESMLSD PHAGVNMPVS FQRRTGDAFT 550 560 570 580 590 600 YFGNTLVTMA MMAYCCDMNT VDCAIFSGDD SLLICKSKPH LDANVFQSLF NMEIKVMDPS 610 620 630 640 650 660 LPYVCSKFLL ETEMNNLVSV PDPMREIQRL AKRKIIKSPE LLRAHFESFC DRMKFLNKLD 670 680 690 700 710 720 EKMINLLCKF VALKYKKPDV ENDVRVAIAA FGYYSENFLR FCECYATEGV NIYKVKHPIT 730 740 750 760 770 780 QEWFEASRDR DGDWFHDWRN PKFPTALDKV WRFFGKYARD DPMKHIEERD RRHRLNRAMN 790 800 SSLKLAYDRR SLSKDKETVA WVRKTLSK

References

[1]	"Sequence of cowpea chlorotic mottle virus RNAs 2 and 3 and evidence of a recombination event during bromovirus evolution." Allison R.F., Janda M., Ahlquist P. Virology 172:321-330(1989) [PubMed: 2773323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M28817 Genomic RNA. Translation: AAA46371.1.
PIR	P2WMCC. JU0117.
RefSeq	NP_613275.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	962150.
Enzyme and pathway databases
BRENDA	2.7.7.48. 290031.
Family and domain databases
InterPro	IPR007610. DUF585. IPR001788. RNA-dep_RNA_pol_vir-typ. IPR007094. RNA_pol_PSvir. [Graphical view]
Pfam	PF04522. DUF585. 1 hit. PF00978. RdRP_2. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ORF2A_CCMV

Accession

Primary (citable) accession number: P20179

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)