Reviewed,
UniProtKB/Swiss-Prot P20171 (RASH_RAT)
Last modified
October 13, 2009.
Version 78.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: GTPase HRas Alternative name(s): Transforming protein p21 p21ras H-Ras-1 c-H-ras Cleaved into the following chain: 1- Recommended name: GTPase HRas, N-terminally processed | ||||
| Gene names |
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| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 189 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. |
| Enzyme regulation | Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). |
| Subunit structure | Forms a signaling complex with RASGRP1 and DGKZ. In its GTP-bound form interacts with PLCE1. Interacts with TBC1D10C. Interacts with RGL3 and RASSF5 By similarity. |
| Subcellular location | Cell membrane; Lipid-anchor; Cytoplasmic side By similarity. Golgi apparatus membrane; Lipid-anchor By similarity. Note: Shuttles between the plasma membrane and the Golgi apparatus By similarity. |
| Post-translational modification | Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi By similarity. S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation. |
| Sequence similarities | Belongs to the small GTPase superfamily. Ras family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Golgi apparatus Membrane |
| Disease | Proto-oncogene |
| Ligand | GTP-binding Nucleotide-binding |
| PTM | Acetylation Lipoprotein Methylation Palmitate Prenylation S-nitrosylation |
| Gene Ontology (GO) | |
| Biological process | Ras protein signal transduction Inferred from mutant phenotype. Source: RGD apoptosisInferred from mutant phenotype. Source: RGD positive regulation of DNA replicationInferred from mutant phenotype. Source: RGD protein heterooligomerizationInferred from physical interaction. Source: RGD |
| Cellular component | Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW anchored to membraneInferred from electronic annotation. Source: UniProtKB-SubCell internal side of plasma membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | GTP binding Inferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 186 | 186 | GTPase HRas | PRO_0000043000 | |||||
| Initiator methionine | 1 | 1 | Removed; alternate By similarity | ||||||
| Chain | 2 – 186 | 185 | GTPase HRas, N-terminally processed | PRO_0000326479 | |||||
| Propeptide | 187 – 189 | 3 | Removed in mature form By similarity | PRO_0000043001 | |||||
Regions | |||||||||
| Nucleotide binding | 10 – 17 | 8 | GTP | ||||||
| Nucleotide binding | 57 – 61 | 5 | GTP | ||||||
| Nucleotide binding | 116 – 119 | 4 | GTP | ||||||
| Region | 166 – 185 | 20 | Hypervariable region | ||||||
| Motif | 32 – 40 | 9 | Effector region | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1 | 1 | N-acetylmethionine; in GTPase HRas; alternate By similarity | ||||||
| Modified residue | 2 | 1 | N-acetylthreonine; in GTPase HRas, N-terminally processed By similarity | ||||||
| Modified residue | 118 | 1 | S-nitrosocysteine By similarity | ||||||
| Modified residue | 186 | 1 | Cysteine methyl ester Ref.3 | ||||||
| Lipidation | 181 | 1 | S-palmitoyl cysteine By similarity | ||||||
| Lipidation | 184 | 1 | S-palmitoyl cysteine | ||||||
| Lipidation | 186 | 1 | S-farnesyl cysteine | ||||||
Experimental info | |||||||||
| Mutagenesis | 26 | 1 | N → G: Interacts and partially stimulates PLCE1; when associated with L-61. Ref.4 | ||||||
| Mutagenesis | 35 | 1 | T → S: No interaction and stimulation of PLCE1; when associated with L-61. Ref.4 | ||||||
| Mutagenesis | 37 | 1 | E → G: Reduced interaction and stimulation of PLCE1; when associated with L-61. Ref.4 | ||||||
| Mutagenesis | 38 | 1 | D → N: Reduced interaction and stimulation of PLCE1; when associated with L-61. Ref.4 | ||||||
| Mutagenesis | 40 | 1 | Y → C: No interaction and stimulation of PLCE1; when associated with L-61. Ref.4 | ||||||
| Mutagenesis | 61 | 1 | Q → L: Constitutively active. Constitutively interacts and stimulates PLCE1 phospholipase activity. Reduced interaction and stimulation of PLCE1; when associated with G-37 and N-38. No interaction and stimulation of PLCE1; when associated with S-35 and C-40. Interacts and partially stimulates PLCE1; when associated with G-26. Interacts but does not stimulate PLCE1; when associated with S-186. Ref.4 | ||||||
| Mutagenesis | 186 | 1 | C → S: Interacts but does not stimulate PLCE1; when associated with L-61. Ref.4 | ||||||
Sequences
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References
| [1] | "Nucleotide sequence of the two rat cellular rasH genes." Ruta M., Wolford R., Dhar R., Defeo-Jones D., Ellis R.W., Scolnick E.M. Mol. Cell. Biol. 6:1706-1710(1986) [PubMed: 3023901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Nucleotide sequence and characterization of the 5' flanking region of the rat Ha-ras protooncogene." Damante G., Filetti S., Rapoport B. Proc. Natl. Acad. Sci. U.S.A. 84:774-778(1987) [PubMed: 3027702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-37. |
| [3] | "Posttranslational modification of the Ha-ras oncogene protein: evidence for a third class of protein carboxyl methyltransferases." Clarke S., Vogel J.P., Deschenes R.J., Stock J. Proc. Natl. Acad. Sci. U.S.A. 85:4643-4647(1988) [PubMed: 3290900] [Abstract] Cited for: ISOPRENYLATION AT CYS-186, CLEAVAGE, METHYLATION AT CYS-186. |
| [4] | "Phospholipase C(epsilon): a novel Ras effector." Kelley G.G., Reks S.E., Ondrako J.M., Smrcka A.V. EMBO J. 20:743-754(2001) [PubMed: 11179219] [Abstract] Cited for: INTERACTION WITH PLCE1, MUTAGENESIS OF ASN-26; THR-35; GLU-37; ASP-38; TYR-40; GLN-61 AND CYS-186. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| M13011 Genomic DNA. Translation: AAA42009.1. M15188 Genomic DNA. Translation: AAA42008.1. | |
| IPI | IPI00196529. |
| PIR | A25229. |
| UniGene | Rn.102180 Rn.129785 |
3D structure databases | |
| HSSP | HSSP built from PDB template 6Q21 based on UniProtKB P01112. |
| SMR | P20171. Positions 1-166. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P20171. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000022363; ENSRNOP00000022363; ENSRNOG00000016611; Rattus norvegicus. [Genome view] |
| UCSC | BC086608. rat. |
Organism-specific databases | |
| RGD | 2827. Hras. |
Phylogenomic databases | |
| HOVERGEN | P20171. |
Gene expression databases | |
| Genevestigator | P20171. |
| GermOnline | ENSRNOG00000016611. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR003577. GTPase_Ras. IPR013753. Ras. IPR001806. Ras_GTPase. IPR015592. Ras_Ras_related. IPR005225. Small_GTP_bd. [Graphical view] |
| PANTHER | PTHR11708:SF125. Ras_Ras_related. 1 hit. |
| Pfam | PF00071. Ras. 1 hit. [Graphical view] |
| PRINTS | PR00449. RASTRNSFRMNG. |
| SMART | SM00173. RAS. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00231. small_GTP. 1 hit. |
| PROSITE | PS51421. RAS. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | RASH_RAT | ||||||||
| Accession | Primary (citable) accession number: P20171 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

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