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Protein

GTPase HRas

Gene

Hras

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17GTP8
Nucleotide bindingi57 – 61GTP5
Nucleotide bindingi116 – 119GTP4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-112412. SOS-mediated signalling.
R-RNO-1169092. Activation of RAS in B cells.
R-RNO-1250347. SHC1 events in ERBB4 signaling.
R-RNO-1433557. Signaling by SCF-KIT.
R-RNO-167044. Signalling to RAS.
R-RNO-171007. p38MAPK events.
R-RNO-179812. GRB2 events in EGFR signaling.
R-RNO-180336. SHC1 events in EGFR signaling.
R-RNO-186763. Downstream signal transduction.
R-RNO-1963640. GRB2 events in ERBB2 signaling.
R-RNO-210993. Tie2 Signaling.
R-RNO-2179392. EGFR Transactivation by Gastrin.
R-RNO-2424491. DAP12 signaling.
R-RNO-2428933. SHC-related events triggered by IGF1R.
R-RNO-2871796. FCERI mediated MAPK activation.
R-RNO-375165. NCAM signaling for neurite out-growth.
R-RNO-3928662. EPHB-mediated forward signaling.
R-RNO-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-RNO-5218921. VEGFR2 mediated cell proliferation.
R-RNO-5621575. CD209 (DC-SIGN) signaling.
R-RNO-5654688. SHC-mediated cascade:FGFR1.
R-RNO-5654693. FRS-mediated FGFR1 signaling.
R-RNO-5654699. SHC-mediated cascade:FGFR2.
R-RNO-5654700. FRS-mediated FGFR2 signaling.
R-RNO-5654704. SHC-mediated cascade:FGFR3.
R-RNO-5654706. FRS-mediated FGFR3 signaling.
R-RNO-5654712. FRS-mediated FGFR4 signaling.
R-RNO-5654719. SHC-mediated cascade:FGFR4.
R-RNO-5658442. Regulation of RAS by GAPs.
R-RNO-5673000. RAF activation.
R-RNO-5673001. RAF/MAP kinase cascade.
R-RNO-5674135. MAP2K and MAPK activation.
R-RNO-5675221. Negative regulation of MAPK pathway.
R-RNO-74751. Insulin receptor signalling cascade.
R-RNO-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.
R-RNO-8851805. MET activates RAS signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase HRas
Alternative name(s):
H-Ras-1
Transforming protein p21
c-H-ras
p21ras
Cleaved into the following chain:
Gene namesi
Name:Hras
Synonyms:Hras1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi2827. Hras.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: RGD
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi26N → G: Interacts and partially stimulates PLCE1; when associated with L-61. 1 Publication1
Mutagenesisi35T → S: No interaction and stimulation of PLCE1; when associated with L-61. 1 Publication1
Mutagenesisi37E → G: Reduced interaction and stimulation of PLCE1; when associated with L-61. 1 Publication1
Mutagenesisi38D → N: Reduced interaction and stimulation of PLCE1; when associated with L-61. 1 Publication1
Mutagenesisi40Y → C: No interaction and stimulation of PLCE1; when associated with L-61. 1 Publication1
Mutagenesisi61Q → L: Constitutively active. Constitutively interacts and stimulates PLCE1 phospholipase activity. Reduced interaction and stimulation of PLCE1; when associated with G-37 and N-38. No interaction and stimulation of PLCE1; when associated with S-35 and C-40. Interacts and partially stimulates PLCE1; when associated with G-26. Interacts but does not stimulate PLCE1; when associated with S-186. 1 Publication1
Mutagenesisi186C → S: Interacts but does not stimulate PLCE1; when associated with L-61. 1 Publication1

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000430001 – 186GTPase HRasAdd BLAST186
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00003264792 – 186GTPase HRas, N-terminally processedAdd BLAST185
PropeptideiPRO_0000043001187 – 189Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylthreonine; in GTPase HRas, N-terminally processedBy similarity1
Modified residuei118S-nitrosocysteineBy similarity1
Lipidationi181S-palmitoyl cysteineBy similarity1
Lipidationi184S-(15-deoxy-Delta12,14-prostaglandin J2-9-yl)cysteine; alternateBy similarity1
Lipidationi184S-palmitoyl cysteine; alternateBy similarity1
Modified residuei186Cysteine methyl ester1 Publication1
Lipidationi186S-farnesyl cysteine1 Publication1

Post-translational modificationi

Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.By similarity
S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.By similarity
The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation.By similarity
Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Palmitate, Prenylation, S-nitrosylation

Proteomic databases

PaxDbiP20171.
PRIDEiP20171.

PTM databases

SwissPalmiP20171.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016611.
GenevisibleiP20171. RN.

Interactioni

Subunit structurei

Forms a signaling complex with RASGRP1 and DGKZ (By similarity). In its GTP-bound form interacts with PLCE1 (PubMed:11179219). Interacts with TBC1D10C (By similarity). Interacts with RGL3 and RASSF5 (By similarity). Interacts with HSPD1 (By similarity). Interacts with PDE6D (By similarity). Interacts with IKZF3 (By similarity). Interacts with RACK1 (By similarity). Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14 (PubMed:19319189). Interacts (active GTP-bound form) with RGS14 (via RBD 1 domain) (PubMed:19319189, PubMed:19878719). Interacts with PIK3CG; the interaction is required for membrane recruitment and beta-gamma G protein dimer-dependent activation of the PI3K gamma complex PIK3CG:PIK3R6 (By similarity). Interacts with RAPGEF2 (By similarity). Interacts (in GTP-bound form) with Oog1 (By similarity).By similarity2 Publications

Protein-protein interaction databases

BioGridi254357. 5 interactors.
IntActiP20171. 1 interactor.
MINTiMINT-4996514.
STRINGi10116.ENSRNOP00000022363.

Structurei

Secondary structure

1189
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 11Combined sources10
Helixi16 – 25Combined sources10
Beta strandi36 – 46Combined sources11
Beta strandi49 – 58Combined sources10
Helixi62 – 64Combined sources3
Helixi68 – 72Combined sources5
Beta strandi76 – 83Combined sources8
Helixi87 – 91Combined sources5
Helixi93 – 104Combined sources12
Beta strandi111 – 116Combined sources6
Helixi127 – 137Combined sources11
Beta strandi141 – 143Combined sources3
Turni146 – 148Combined sources3
Helixi152 – 163Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3V4FX-ray1.39A1-166[»]
ProteinModelPortaliP20171.
SMRiP20171.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 185Hypervariable regionAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector region9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133672.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP20171.
KOiK02833.
OMAiDCMNCKC.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20171-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET
60 70 80 90 100
CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI
110 120 130 140 150
KRVKDSDDVP MVLVGNKCDL AARTVESRQA QDLARSYGIP YIETSAKTRQ
160 170 180
GVEDAFYTLV REIRQHKLRK LNPPDESGPG CMSCKCVLS
Length:189
Mass (Da):21,298
Last modified:July 27, 2011 - v2
Checksum:iEE6DC2D933E2856A
GO

Sequence cautioni

The sequence AAH86608 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH99130 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti179P → L in AAA42009 (PubMed:3023901).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13011 Genomic DNA. Translation: AAA42009.1.
BC086608 mRNA. Translation: AAH86608.1. Different initiation.
BC099130 mRNA. Translation: AAH99130.1. Different initiation.
M15188 Genomic DNA. Translation: AAA42008.1.
PIRiA25229.
RefSeqiNP_001091711.1. NM_001098241.1.
NP_001123913.1. NM_001130441.1.
UniGeneiRn.102180.

Genome annotation databases

EnsembliENSRNOT00000022363; ENSRNOP00000022363; ENSRNOG00000016611.
ENSRNOT00000079464; ENSRNOP00000069770; ENSRNOG00000016611.
GeneIDi293621.
KEGGirno:293621.
UCSCiRGD:2827. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13011 Genomic DNA. Translation: AAA42009.1.
BC086608 mRNA. Translation: AAH86608.1. Different initiation.
BC099130 mRNA. Translation: AAH99130.1. Different initiation.
M15188 Genomic DNA. Translation: AAA42008.1.
PIRiA25229.
RefSeqiNP_001091711.1. NM_001098241.1.
NP_001123913.1. NM_001130441.1.
UniGeneiRn.102180.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3V4FX-ray1.39A1-166[»]
ProteinModelPortaliP20171.
SMRiP20171.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi254357. 5 interactors.
IntActiP20171. 1 interactor.
MINTiMINT-4996514.
STRINGi10116.ENSRNOP00000022363.

PTM databases

SwissPalmiP20171.

Proteomic databases

PaxDbiP20171.
PRIDEiP20171.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022363; ENSRNOP00000022363; ENSRNOG00000016611.
ENSRNOT00000079464; ENSRNOP00000069770; ENSRNOG00000016611.
GeneIDi293621.
KEGGirno:293621.
UCSCiRGD:2827. rat.

Organism-specific databases

CTDi3265.
RGDi2827. Hras.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133672.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP20171.
KOiK02833.
OMAiDCMNCKC.

Enzyme and pathway databases

ReactomeiR-RNO-112412. SOS-mediated signalling.
R-RNO-1169092. Activation of RAS in B cells.
R-RNO-1250347. SHC1 events in ERBB4 signaling.
R-RNO-1433557. Signaling by SCF-KIT.
R-RNO-167044. Signalling to RAS.
R-RNO-171007. p38MAPK events.
R-RNO-179812. GRB2 events in EGFR signaling.
R-RNO-180336. SHC1 events in EGFR signaling.
R-RNO-186763. Downstream signal transduction.
R-RNO-1963640. GRB2 events in ERBB2 signaling.
R-RNO-210993. Tie2 Signaling.
R-RNO-2179392. EGFR Transactivation by Gastrin.
R-RNO-2424491. DAP12 signaling.
R-RNO-2428933. SHC-related events triggered by IGF1R.
R-RNO-2871796. FCERI mediated MAPK activation.
R-RNO-375165. NCAM signaling for neurite out-growth.
R-RNO-3928662. EPHB-mediated forward signaling.
R-RNO-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-RNO-5218921. VEGFR2 mediated cell proliferation.
R-RNO-5621575. CD209 (DC-SIGN) signaling.
R-RNO-5654688. SHC-mediated cascade:FGFR1.
R-RNO-5654693. FRS-mediated FGFR1 signaling.
R-RNO-5654699. SHC-mediated cascade:FGFR2.
R-RNO-5654700. FRS-mediated FGFR2 signaling.
R-RNO-5654704. SHC-mediated cascade:FGFR3.
R-RNO-5654706. FRS-mediated FGFR3 signaling.
R-RNO-5654712. FRS-mediated FGFR4 signaling.
R-RNO-5654719. SHC-mediated cascade:FGFR4.
R-RNO-5658442. Regulation of RAS by GAPs.
R-RNO-5673000. RAF activation.
R-RNO-5673001. RAF/MAP kinase cascade.
R-RNO-5674135. MAP2K and MAPK activation.
R-RNO-5675221. Negative regulation of MAPK pathway.
R-RNO-74751. Insulin receptor signalling cascade.
R-RNO-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.
R-RNO-8851805. MET activates RAS signaling.

Miscellaneous databases

PROiP20171.

Gene expression databases

BgeeiENSRNOG00000016611.
GenevisibleiP20171. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRASH_RAT
AccessioniPrimary (citable) accession number: P20171
Secondary accession number(s): Q4KLL6, Q5RJJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.