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Protein

Drug sensory protein A

Gene

dspA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphorelay sensor kinase activity Source: InterPro

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: InterPro
  2. response to herbicide Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Herbicide resistance, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.13.3. 6185.

Names & Taxonomyi

Protein namesi
Recommended name:
Drug sensory protein A (EC:2.7.13.3)
Gene namesi
Name:dspA
Synonyms:dfr
Ordered Locus Names:sll0698
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei32 – 5221HelicalSequence AnalysisAdd
BLAST
Transmembranei165 – 18521HelicalSequence AnalysisAdd
BLAST
Transmembranei199 – 21921HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 663663Drug sensory protein APRO_0000074758Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei432 – 4321Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP20169.
PRIDEiP20169.

Interactioni

Protein-protein interaction databases

IntActiP20169. 1 interaction.
STRINGi1148.sll0698.

Structurei

3D structure databases

ProteinModelPortaliP20169.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini220 – 27253HAMPPROSITE-ProRule annotationAdd
BLAST
Domaini281 – 35171PASPROSITE-ProRule annotationAdd
BLAST
Domaini429 – 656228Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0642.
HOGENOMiHOG000233124.
InParanoidiP20169.
KOiK07769.
OMAiAEKFWRS.
OrthoDBiEOG6G4VQG.
PhylomeDBiP20169.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003661. EnvZ-like_dim/P.
IPR003660. HAMP_linker_domain.
IPR003594. HATPase_C.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20169-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTSVSNPTA ILQTMQGFLR KWWSEFNLQT RLMAAATLVV SLLMSGLTFW
60 70 80 90 100
AVNTIQEDAQ LVDTRFGRDV GLLLAANVAP MIADKNLTEV ARFSSRFYEN
110 120 130 140 150
TSNIRYMIYA DPSGKIFFGI PYSEETVQNS LTLERRIELP QIDPHNFDQP
160 170 180 190 200
FVRQHHTPNG DVTDVFIPLQ YQGKFLGVLA IGINPNPAAV NSSNLTRDVT
210 220 230 240 250
IAVFISIWVM VILGAVFNAL TITQPIKELL LGVKNIAAGN FKQRITLPFG
260 270 280 290 300
GELGELIVNF NEMAERLERY EAQNIEELTA EKAKLDTLVS TIADGAMLVD
310 320 330 340 350
TNLQLLLVNP TARRLFAWEN KPIIGENLLE NLPPEITAQL TQPLRELAAD
360 370 380 390 400
QGSLLFSPGH GPQEEEQDKT YAPEEFRISL TQPFPRTIRL MLTQVLDQNR
410 420 430 440 450
ENLRGIVMTV QDITREVELN EAKSQFISNV SHELRTPLFN IKSFIETLSE
460 470 480 490 500
FGEDLSEVER KEFLETANHE TDRLSRLVND VLDLSKLESS KIYQLDAVDL
510 520 530 540 550
YQLIEQSLRS YQLNAKDKQL QLEKILDPDL PFALGNYDLL LQVMTNLIGN
560 570 580 590 600
SFKFTKAGGK IIVRAYPLHR SNLRAEDGPG LVRVEISDTG IGIDPEDQAA
610 620 630 640 650
IFERFYRVEN RVHTLEGTGL GLSIVKNIIA KHQSQIHLVS EVGVGTTFWF
660
DLAVYQSMLM VVG
Length:663
Mass (Da):74,536
Last modified:November 1, 1997 - v2
Checksum:i42CD7CA67E396586
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72856 Genomic DNA. Translation: CAA51377.1.
BA000022 Genomic DNA. Translation: BAA16687.1.
J04195 Genomic DNA. No translation available.
PIRiS74535.
RefSeqiNP_440007.1. NC_000911.1.
YP_005650063.1. NC_017277.1.
YP_007449890.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA16687; BAA16687; BAA16687.
GeneIDi953218.
KEGGisyn:sll0698.
PATRICi23837088. VBISynSp132158_0119.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72856 Genomic DNA. Translation: CAA51377.1.
BA000022 Genomic DNA. Translation: BAA16687.1.
J04195 Genomic DNA. No translation available.
PIRiS74535.
RefSeqiNP_440007.1. NC_000911.1.
YP_005650063.1. NC_017277.1.
YP_007449890.1. NC_020286.1.

3D structure databases

ProteinModelPortaliP20169.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP20169. 1 interaction.
STRINGi1148.sll0698.

Proteomic databases

PaxDbiP20169.
PRIDEiP20169.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA16687; BAA16687; BAA16687.
GeneIDi953218.
KEGGisyn:sll0698.
PATRICi23837088. VBISynSp132158_0119.

Phylogenomic databases

eggNOGiCOG0642.
HOGENOMiHOG000233124.
InParanoidiP20169.
KOiK07769.
OMAiAEKFWRS.
OrthoDBiEOG6G4VQG.
PhylomeDBiP20169.

Enzyme and pathway databases

BRENDAi2.7.13.3. 6185.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003661. EnvZ-like_dim/P.
IPR003660. HAMP_linker_domain.
IPR003594. HATPase_C.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The dspA gene product of the cyanobacterium Synechocystis sp. strain PCC 6803 influences sensitivity to chemically different growth inhibitors and has amino acid similarity to histidine protein kinases."
    Bartsevich V.V., Shestakov S.V.
    Microbiology 141:2915-2920(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.
  3. "Molecular cloning and sequencing of the psaD gene encoding subunit II of photosystem I from the cyanobacterium, Synechocystis sp. PCC 6803."
    Reilly P., Hulmes J.D., Pan Y.-C.E., Nelson N.
    J. Biol. Chem. 263:17658-17662(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.

Entry informationi

Entry nameiDSPA_SYNY3
AccessioniPrimary (citable) accession number: P20169
Secondary accession number(s): Q55232
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutations in dspA results in resistance to herbicides difunon, diuron and calmodulin antagonists chlorpromazine and trifluoperazine.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.