P20169 (DSPA_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Drug sensory protein A
EC=2.7.13.3

Gene names

Name:	dspA
Synonyms:	dfr
Ordered Locus Names:	sll0698

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	663 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Miscellaneous	Mutations in dspA results in resistance to herbicides difunon, diuron and calmodulin antagonists chlorpromazine and trifluoperazine.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Biological process	Herbicide resistance Two-component regulatory system
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro response to herbicide Inferred from electronic annotation. Source: UniProtKB-KW signal transduction by phosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay sensor kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 663	663	Drug sensory protein A		PRO_0000074758
Regions
	Transmembrane	32 – 52	21	Helical; Potential
	Transmembrane	165 – 185	21	Helical; Potential
	Transmembrane	199 – 219	21	Helical; Potential
	Domain	220 – 272	53	HAMP
	Domain	281 – 351	71	PAS
	Domain	429 – 656	228	Histidine kinase
Amino acid modifications
	Modified residue	432	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20169 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 42CD7CA67E396586	FASTA	663	74,536
	10 20 30 40 50 60 MGTSVSNPTA ILQTMQGFLR KWWSEFNLQT RLMAAATLVV SLLMSGLTFW AVNTIQEDAQ 70 80 90 100 110 120 LVDTRFGRDV GLLLAANVAP MIADKNLTEV ARFSSRFYEN TSNIRYMIYA DPSGKIFFGI 130 140 150 160 170 180 PYSEETVQNS LTLERRIELP QIDPHNFDQP FVRQHHTPNG DVTDVFIPLQ YQGKFLGVLA 190 200 210 220 230 240 IGINPNPAAV NSSNLTRDVT IAVFISIWVM VILGAVFNAL TITQPIKELL LGVKNIAAGN 250 260 270 280 290 300 FKQRITLPFG GELGELIVNF NEMAERLERY EAQNIEELTA EKAKLDTLVS TIADGAMLVD 310 320 330 340 350 360 TNLQLLLVNP TARRLFAWEN KPIIGENLLE NLPPEITAQL TQPLRELAAD QGSLLFSPGH 370 380 390 400 410 420 GPQEEEQDKT YAPEEFRISL TQPFPRTIRL MLTQVLDQNR ENLRGIVMTV QDITREVELN 430 440 450 460 470 480 EAKSQFISNV SHELRTPLFN IKSFIETLSE FGEDLSEVER KEFLETANHE TDRLSRLVND 490 500 510 520 530 540 VLDLSKLESS KIYQLDAVDL YQLIEQSLRS YQLNAKDKQL QLEKILDPDL PFALGNYDLL 550 560 570 580 590 600 LQVMTNLIGN SFKFTKAGGK IIVRAYPLHR SNLRAEDGPG LVRVEISDTG IGIDPEDQAA 610 620 630 640 650 660 IFERFYRVEN RVHTLEGTGL GLSIVKNIIA KHQSQIHLVS EVGVGTTFWF DLAVYQSMLM VVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The dspA gene product of the cyanobacterium Synechocystis sp. strain PCC 6803 influences sensitivity to chemically different growth inhibitors and has amino acid similarity to histidine protein kinases." Bartsevich V.V., Shestakov S.V. Microbiology 141:2915-2920(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.
[3]	"Molecular cloning and sequencing of the psaD gene encoding subunit II of photosystem I from the cyanobacterium, Synechocystis sp. PCC 6803." Reilly P., Hulmes J.D., Pan Y.-C.E., Nelson N. J. Biol. Chem. 263:17658-17662(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X72856 Genomic DNA. Translation: CAA51377.1. BA000022 Genomic DNA. Translation: BAA16687.1. J04195 Genomic DNA. No translation available.
PIR	S74535.
RefSeq	NP_440007.1. NC_000911.1. YP_005650063.1. NC_017277.1. YP_007449890.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P20169.
ModBase	Search...
Protein-protein interaction databases
IntAct	P20169. 1 interaction.
STRING	1148.sll0698.
Proteomic databases
PaxDb	P20169.
PRIDE	P20169.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA16687; BAA16687; BAA16687.
GeneID	12253896. 14615531. 953218.
KEGG	syn:sll0698. syy:SYNGTS_0110.
PATRIC	23837088. VBISynSp132158_0119.
Phylogenomic databases
eggNOG	COG0642.
HOGENOM	HOG000233124.
KO	K07769.
OMA	NDTRYAR.
ProtClustDB	CLSK892481.
Enzyme and pathway databases
BRENDA	2.7.13.3. 6185.
Family and domain databases
Gene3D	1.10.287.130. 1 hit. 3.30.565.10. 1 hit.
InterPro	IPR003660. HAMP_linker_domain. IPR003594. HATPase_ATP-bd. IPR000014. PAS. IPR013767. PAS_fold. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. [Graphical view]
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. PF00989. PAS. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF47384. His_kin_homodim. 1 hit.
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. PS50112. PAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DSPA_SYNY3

Accession

Primary (citable) accession number: P20169
Secondary accession number(s): Q55232

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program