Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

N-(5'-phosphoribosyl)anthranilate isomerase

Gene

trpF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase component 1 (trpE), Aminodeoxychorismate/anthranilate synthase component 2 (pabA)
  2. Anthranilate phosphoribosyltransferase (trpD), Anthranilate phosphoribosyltransferase (trpD)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF), N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (trpC), Indole-3-glycerol phosphate synthase (trpC)
  5. Tryptophan synthase alpha chain (trpA), Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB), Tryptophan synthase beta chain (trpB)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU22650-MONOMER.
UniPathwayiUPA00035; UER00042.

Names & Taxonomyi

Protein namesi
Recommended name:
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:trpF
Ordered Locus Names:BSU22650
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001543471 – 215N-(5'-phosphoribosyl)anthranilate isomeraseAdd BLAST215

Proteomic databases

PaxDbiP20167.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012441.

Structurei

3D structure databases

ProteinModelPortaliP20167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpF family.Curated

Phylogenomic databases

eggNOGiENOG4108ZGB. Bacteria.
COG0135. LUCA.
HOGENOMiHOG000161598.
InParanoidiP20167.
KOiK01817.
OMAiRCFIAGG.
PhylomeDBiP20167.

Family and domain databases

CDDicd00405. PRAI. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00135. PRAI. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00697. PRAI. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.

Sequencei

Sequence statusi: Complete.

P20167-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKPALKYCG IRSLKDLQLA AESQADYLGF IFAESKRKVS PEDVKKWLNQ
60 70 80 90 100
VRVEKQVAGV FVNESIETMS RIAKSLKLDV IQLHGDEKPA DAAALRKLTG
110 120 130 140 150
CEIWKALHHQ DNTTQEIARF KDNVDGFVID SSVKGSRGGT GVAFSWECVP
160 170 180 190 200
EYQQAAIGKR CFIAGGVNPD SITRLLKWQP EGIDLASGIE KNGQKDQNLM
210
RLLEERMNRY VSISE
Length:215
Mass (Da):24,041
Last modified:July 28, 2009 - v3
Checksum:i0153C28BA53DC9ED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15K → Q in AAA20864 (PubMed:3924737).Curated1
Sequence conflicti15K → Q in AAA22868 (PubMed:3924737).Curated1
Sequence conflicti22E → D in AAA20864 (PubMed:3924737).Curated1
Sequence conflicti22E → D in AAA22868 (PubMed:3924737).Curated1
Sequence conflicti36K → Y in AAA20864 (PubMed:3924737).Curated1
Sequence conflicti36K → Y in AAA22868 (PubMed:3924737).Curated1
Sequence conflicti42E → Q in AAA20864 (PubMed:3924737).Curated1
Sequence conflicti42E → Q in AAA22868 (PubMed:3924737).Curated1
Sequence conflicti49N → S in AAA20864 (PubMed:3924737).Curated1
Sequence conflicti49N → S in AAA22868 (PubMed:3924737).Curated1
Sequence conflicti52R → C in AAA20864 (PubMed:3924737).Curated1
Sequence conflicti52R → C in AAA22868 (PubMed:3924737).Curated1
Sequence conflicti74 – 77KSLK → EDLN in AAA20864 (PubMed:3924737).Curated4
Sequence conflicti74 – 77KSLK → EDLN in AAA22868 (PubMed:3924737).Curated4
Sequence conflicti88K → E in AAA20864 (PubMed:3924737).Curated1
Sequence conflicti88K → E in AAA22868 (PubMed:3924737).Curated1
Sequence conflicti93A → D in AAA20864 (PubMed:3924737).Curated1
Sequence conflicti93A → D in AAA22868 (PubMed:3924737).Curated1
Sequence conflicti97K → M in AAA20864 (PubMed:3924737).Curated1
Sequence conflicti97K → M in AAA22868 (PubMed:3924737).Curated1
Sequence conflicti100G → D in AAA20864 (PubMed:3924737).Curated1
Sequence conflicti100G → D in AAA22868 (PubMed:3924737).Curated1
Sequence conflicti110 – 111QD → HE in AAA20864 (PubMed:3924737).Curated2
Sequence conflicti110 – 111QD → HE in AAA22868 (PubMed:3924737).Curated2
Sequence conflicti123N → D in AAA20864 (PubMed:3924737).Curated1
Sequence conflicti123N → D in AAA22868 (PubMed:3924737).Curated1
Sequence conflicti136S → A in AAA20864 (PubMed:3924737).Curated1
Sequence conflicti136S → A in AAA22868 (PubMed:3924737).Curated1
Sequence conflicti147E → D in AAA20864 (PubMed:3924737).Curated1
Sequence conflicti147E → D in AAA22868 (PubMed:3924737).Curated1
Sequence conflicti171S → T in AAA20864 (PubMed:3924737).Curated1
Sequence conflicti171S → T in AAA22868 (PubMed:3924737).Curated1
Sequence conflicti174 – 178RLLKW → DLMKY in AAA20864 (PubMed:3924737).Curated5
Sequence conflicti174 – 178RLLKW → DLMKY in AAA22868 (PubMed:3924737).Curated5
Sequence conflicti181E → A in AAA20864 (PubMed:3924737).Curated1
Sequence conflicti181E → A in AAA22868 (PubMed:3924737).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80245 Genomic DNA. Translation: AAA20864.1.
K01391 Genomic DNA. Translation: AAA22868.1.
AL009126 Genomic DNA. Translation: CAB14181.3.
PIRiD22794.
RefSeqiNP_390146.3. NC_000964.3.
WP_004398742.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14181; CAB14181; BSU22650.
GeneIDi939006.
KEGGibsu:BSU22650.
PATRICi18976339. VBIBacSub10457_2360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80245 Genomic DNA. Translation: AAA20864.1.
K01391 Genomic DNA. Translation: AAA22868.1.
AL009126 Genomic DNA. Translation: CAB14181.3.
PIRiD22794.
RefSeqiNP_390146.3. NC_000964.3.
WP_004398742.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP20167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012441.

Proteomic databases

PaxDbiP20167.

Protocols and materials databases

DNASUi939006.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14181; CAB14181; BSU22650.
GeneIDi939006.
KEGGibsu:BSU22650.
PATRICi18976339. VBIBacSub10457_2360.

Phylogenomic databases

eggNOGiENOG4108ZGB. Bacteria.
COG0135. LUCA.
HOGENOMiHOG000161598.
InParanoidiP20167.
KOiK01817.
OMAiRCFIAGG.
PhylomeDBiP20167.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00042.
BioCyciBSUB:BSU22650-MONOMER.

Family and domain databases

CDDicd00405. PRAI. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00135. PRAI. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00697. PRAI. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRPF_BACSU
AccessioniPrimary (citable) accession number: P20167
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 28, 2009
Last modified: October 5, 2016
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.