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Protein

PTS system glucose-specific EIICBA component

Gene

ptsG

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport.By similarity

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-glucose(Side 1) = [protein]-L-histidine + D-glucose 6-phosphate(Side 2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei461Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation1
Active sitei620Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciBSUB:BSU13890-MONOMER.
SABIO-RKP20166.

Protein family/group databases

TCDBi4.A.1.1.9. the pts glucose-glucoside (glc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system glucose-specific EIICBA componentBy similarity (EC:2.7.1.199By similarity)
Alternative name(s):
EII-Glc/EIII-Glc1 Publication
EIICBA-GlcBy similarity
EIICBA-Glc 1Curated
Including the following 3 domains:
Glucose permease IIC componentBy similarity
Alternative name(s):
PTS system glucose-specific EIIC componentBy similarity
Glucose-specific phosphotransferase enzyme IIB componentBy similarity
Alternative name(s):
PTS system glucose-specific EIIB componentBy similarity
Glucose-specific phosphotransferase enzyme IIA componentBy similarity
Alternative name(s):
PTS system glucose-specific EIIA componentBy similarity
Gene namesi
Name:ptsG
Synonyms:crr, ptsX
Ordered Locus Names:BSU13890
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei16 – 36HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei66 – 86HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei89 – 109HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei139 – 159HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei180 – 200HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei233 – 253HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei283 – 303HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei313 – 333HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei338 – 358HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei365 – 385HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei388 – 408HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865571 – 699PTS system glucose-specific EIICBA componentAdd BLAST699

Proteomic databases

PaxDbiP20166.
PRIDEiP20166.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100007711.

Structurei

Secondary structure

1699
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi557 – 562Combined sources6
Helixi563 – 565Combined sources3
Beta strandi566 – 568Combined sources3
Helixi569 – 572Combined sources4
Beta strandi577 – 584Combined sources8
Beta strandi586 – 592Combined sources7
Beta strandi595 – 599Combined sources5
Beta strandi602 – 614Combined sources13
Beta strandi616 – 620Combined sources5
Beta strandi622 – 624Combined sources3
Turni625 – 632Combined sources8
Beta strandi633 – 636Combined sources4
Beta strandi641 – 643Combined sources3
Beta strandi645 – 652Combined sources8
Helixi654 – 657Combined sources4
Helixi658 – 660Combined sources3
Beta strandi666 – 672Combined sources7
Helixi673 – 675Combined sources3
Beta strandi678 – 681Combined sources4
Beta strandi685 – 687Combined sources3
Beta strandi692 – 698Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AX3NMR-A539-699[»]
1GPRX-ray1.90A539-699[»]
ProteinModelPortaliP20166.
SMRiP20166.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20166.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 424PTS EIIC type-1PROSITE-ProRule annotationAdd BLAST424
Domaini439 – 520PTS EIIB type-1PROSITE-ProRule annotationAdd BLAST82
Domaini568 – 672PTS EIIA type-1PROSITE-ProRule annotationAdd BLAST105

Domaini

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation
The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.PROSITE-ProRule annotation
The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIA type-1 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIB type-1 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1263. LUCA.
COG1264. LUCA.
COG2190. LUCA.
HOGENOMiHOG000250993.
InParanoidiP20166.
KOiK20116.
K20117.
K20118.
OMAiMQIGEFT.
PhylomeDBiP20166.

Family and domain databases

CDDicd00212. PTS_IIB_glc. 1 hit.
Gene3Di3.30.1360.60. 1 hit.
InterProiIPR011055. Dup_hybrid_motif.
IPR018113. PTrfase_EIIB_Cys.
IPR001127. PTS_EIIA_1_perm.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR011299. PTS_IIBC_glc.
IPR004719. PTS_maltose/Glc_sub_IIC.
[Graphical view]
PfamiPF00358. PTS_EIIA_1. 1 hit.
PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
SSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR00830. PTBA. 1 hit.
TIGR00852. pts-Glc. 1 hit.
TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITEiPS51093. PTS_EIIA_TYPE_1. 1 hit.
PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKALFGVLQ KIGRALMLPV AILPAAGILL AIGNAMQNKD MIQVLHFLSN
60 70 80 90 100
DNVQLVAGVM ESAGQIVFDN LPLLFAVGVA IGLANGDGVA GIAAIIGYLV
110 120 130 140 150
MNVSMSAVLL ANGTIPSDSV ERAKFFTENH PAYVNMLGIP TLATGVFGGI
160 170 180 190 200
IVGVLAALLF NRFYTIELPQ YLGFFAGKRF VPIVTSISAL ILGLIMLVIW
210 220 230 240 250
PPIQHGLNAF STGLVEANPT LAAFIFGVIE RSLIPFGLHH IFYSPFWYEF
260 270 280 290 300
FSYKSAAGEI IRGDQRIFMA QIKDGVQLTA GTFMTGKYPF MMFGLPAAAL
310 320 330 340 350
AIYHEAKPQN KKLVAGIMGS AALTSFLTGI TEPLEFSFLF VAPVLFAIHC
360 370 380 390 400
LFAGLSFMVM QLLNVKIGMT FSGGLIDYFL FGILPNRTAW WLVIPVGLGL
410 420 430 440 450
AVIYYFGFRF AIRKFNLKTP GREDAAEETA APGKTGEAGD LPYEILQAMG
460 470 480 490 500
DQENIKHLDA CITRLRVTVN DQKKVDKDRL KQLGASGVLE VGNNIQAIFG
510 520 530 540 550
PRSDGLKTQM QDIIAGRKPR PEPKTSAQEE VGQQVEEVIA EPLQNEIGEE
560 570 580 590 600
VFVSPITGEI HPITDVPDQV FSGKMMGDGF AILPSEGIVV SPVRGKILNV
610 620 630 640 650
FPTKHAIGLQ SDGGREILIH FGIDTVSLKG EGFTSFVSEG DRVEPGQKLL
660 670 680 690
EVDLDAVKPN VPSLMTPIVF TNLAEGETVS IKASGSVNRE QEDIVKIEK
Length:699
Mass (Da):75,525
Last modified:December 1, 1992 - v2
Checksum:i2A14D3C32EE0A9C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11744 Genomic DNA. Translation: CAA77803.1.
AL009126 Genomic DNA. Translation: CAB13262.1.
X12832 Genomic DNA. Translation: CAA31315.1.
M60344 Genomic DNA. Translation: AAA22498.1.
PIRiS25083. WQBSGS.
RefSeqiNP_389272.1. NC_000964.3.
WP_003244661.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13262; CAB13262; BSU13890.
GeneIDi939255.
KEGGibsu:BSU13890.
PATRICi18974561. VBIBacSub10457_1472.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11744 Genomic DNA. Translation: CAA77803.1.
AL009126 Genomic DNA. Translation: CAB13262.1.
X12832 Genomic DNA. Translation: CAA31315.1.
M60344 Genomic DNA. Translation: AAA22498.1.
PIRiS25083. WQBSGS.
RefSeqiNP_389272.1. NC_000964.3.
WP_003244661.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AX3NMR-A539-699[»]
1GPRX-ray1.90A539-699[»]
ProteinModelPortaliP20166.
SMRiP20166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100007711.

Protein family/group databases

TCDBi4.A.1.1.9. the pts glucose-glucoside (glc) family.

Proteomic databases

PaxDbiP20166.
PRIDEiP20166.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13262; CAB13262; BSU13890.
GeneIDi939255.
KEGGibsu:BSU13890.
PATRICi18974561. VBIBacSub10457_1472.

Phylogenomic databases

eggNOGiCOG1263. LUCA.
COG1264. LUCA.
COG2190. LUCA.
HOGENOMiHOG000250993.
InParanoidiP20166.
KOiK20116.
K20117.
K20118.
OMAiMQIGEFT.
PhylomeDBiP20166.

Enzyme and pathway databases

BioCyciBSUB:BSU13890-MONOMER.
SABIO-RKP20166.

Miscellaneous databases

EvolutionaryTraceiP20166.

Family and domain databases

CDDicd00212. PTS_IIB_glc. 1 hit.
Gene3Di3.30.1360.60. 1 hit.
InterProiIPR011055. Dup_hybrid_motif.
IPR018113. PTrfase_EIIB_Cys.
IPR001127. PTS_EIIA_1_perm.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR011299. PTS_IIBC_glc.
IPR004719. PTS_maltose/Glc_sub_IIC.
[Graphical view]
PfamiPF00358. PTS_EIIA_1. 1 hit.
PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
SSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR00830. PTBA. 1 hit.
TIGR00852. pts-Glc. 1 hit.
TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITEiPS51093. PTS_EIIA_TYPE_1. 1 hit.
PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTG3C_BACSU
AccessioniPrimary (citable) accession number: P20166
Secondary accession number(s): P08875
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.