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Protein

Defensin-like protein 1

Gene
N/A
Organism
Triticum aestivum (Wheat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits protein translation in cell-free systems.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Fungicide

Keywords - Biological processi

Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Defensin-like protein 1
Alternative name(s):
Gamma-1-purothionin
OrganismiTriticum aestivum (Wheat)
Taxonomic identifieri4565 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeTriticinaeTriticum
Proteomesi
  • UP000019116 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4747Defensin-like protein 1PRO_0000074261Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi3 ↔ 47
Disulfide bondi14 ↔ 34
Disulfide bondi20 ↔ 41
Disulfide bondi24 ↔ 43

Keywords - PTMi

Disulfide bond

Interactioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei11 – 111Interaction with trypsinBy similarity

Structurei

Secondary structure

1
47
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Helixi17 – 2610Combined sources
Beta strandi30 – 356Combined sources
Turni36 – 394Combined sources
Beta strandi40 – 467Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GPSNMR-A1-47[»]
ProteinModelPortaliP20158.
SMRiP20158. Positions 1-47.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20158.

Family & Domainsi

Sequence similaritiesi

Belongs to the DEFL family.Curated

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR008177. G_Purothionin.
IPR008176. Gamma-thionin.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PRINTSiPR00288. PUROTHIONIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS00940. GAMMA_THIONIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20158-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40 
KICRRRSAGF KGPCMSNKNC AQVCQQEGWG GGNCDGPFRR CKCIRQC
Length:47
Mass (Da):5,239
Last modified:February 1, 1991 - v1
Checksum:i8ACB73627381A4D8
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GPSNMR-A1-47[»]
ProteinModelPortaliP20158.
SMRiP20158. Positions 1-47.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP20158.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR008177. G_Purothionin.
IPR008176. Gamma-thionin.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PRINTSiPR00288. PUROTHIONIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS00940. GAMMA_THIONIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Gamma-purothionins: amino acid sequence of two polypeptides of a new family of thionins from wheat endosperm."
    Colilla F.J., Rocher A., Mendez E.
    FEBS Lett. 270:191-194(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Strain: cv. Senatore Capelli.
    Tissue: Endosperm.
  2. "Solution structure of gamma 1-H and gamma 1-P thionins from barley and wheat endosperm determined by 1H-NMR: a structural motif common to toxic arthropod proteins."
    Bruix M., Jimenez M.A., Santoro J., Gonzalez C., Colilla F.J., Mendez E., Rico M.
    Biochemistry 32:715-724(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiDEF1_WHEAT
AccessioniPrimary (citable) accession number: P20158
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: February 17, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was initially thought (PubMed:2226781 and PubMed:8380707) to be a thionin.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.