Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Neurosecretory protein VGF

Gene

Vgf

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.
NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release. NERP-2 stimulates feeding behavior in an orexin-dependent manner.1 Publication
TLQP-21 peptide plays a central and local role in the regulation of rat gastric motor functions.1 Publication

GO - Biological processi

  • neuron differentiation Source: RGD
  • penile erection Source: RGD
  • regulation of neuronal synaptic plasticity Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Growth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Neurosecretory protein VGF
Alternative name(s):
VGF8a protein
Cleaved into the following 12 chains:
Gene namesi
Name:Vgf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69399. Vgf.

Subcellular locationi

  • Secreted 1 Publication
  • Cytoplasmic vesiclesecretory vesicle 1 Publication

  • Note: Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.

GO - Cellular componenti

  • extracellular region Source: UniProtKB-SubCell
  • neuronal cell body Source: RGD
  • neuron projection Source: RGD
  • secretory granule membrane Source: RGD
  • transport vesicle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 617594Neurosecretory protein VGFPRO_0000022656Add
BLAST
Peptidei24 – 6340VGF(24-63)PRO_0000375995Add
BLAST
Peptidei180 – 19415VGF(180-194)PRO_0000375996Add
BLAST
Peptidei285 – 30925Neuroendocrine regulatory peptide-1PRO_0000403366Add
BLAST
Peptidei313 – 35038Neuroendocrine regulatory peptide-2PRO_0000403367Add
BLAST
Peptidei375 – 40733VGF(375-407)PRO_0000375997Add
BLAST
Peptidei556 – 61762TLQP-62PRO_0000403368Add
BLAST
Peptidei556 – 58732TLQP-30PRO_0000403369Add
BLAST
Peptidei556 – 57621TLQP-21PRO_0000403370Add
BLAST
Peptidei556 – 56611TLQP-11PRO_0000403371Add
BLAST
Peptidei567 – 57610HFHH-10PRO_0000403372
Peptidei588 – 61730AQEE-30PRO_0000403373Add
BLAST
Peptidei599 – 61719LQEQ-19PRO_0000403374Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei180 – 1801Pyrrolidone carboxylic acid1 Publication
Modified residuei313 – 3131Pyrrolidone carboxylic acid1 Publication
Modified residuei423 – 4231PhosphoserineBy similarity

Post-translational modificationi

Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure.

Keywords - PTMi

Cleavage on pair of basic residues, Phosphoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP20156.
PRIDEiP20156.

Expressioni

Tissue specificityi

Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.1 Publication

Inductioni

By nerve growth factor.

Interactioni

Protein-protein interaction databases

BioGridi248104. 1 interaction.
IntActiP20156. 1 interaction.
MINTiMINT-7139146.
STRINGi10116.ENSRNOP00000050024.

Structurei

3D structure databases

ProteinModelPortaliP20156.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi360 – 45293Asp/Glu-rich (acidic)Add
BLAST

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IKTZ. Eukaryota.
ENOG410YR85. LUCA.
HOGENOMiHOG000120127.
HOVERGENiHBG006806.
InParanoidiP20156.
PhylomeDBiP20156.

Family and domain databases

InterProiIPR026128. VGF.
[Graphical view]
PANTHERiPTHR15159. PTHR15159. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20156-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTFTLPASV LFCFLLLIRG LGAAPPGRSD VYPPPLGSEH NGQVAEDAVS
60 70 80 90 100
RPKDDSVPEV RAARNSEPQD QGELFQGVDP RALAAVLLQA LDRPASPPAV
110 120 130 140 150
PAGSQQGTPE EAAEALLTES VRSQTHSLPA SEIQASAVAP PRPQTQDNDP
160 170 180 190 200
EADDRSEELE ALASLLQELR DFSPSNAKRQ QETAAAETET RTHTLTRVNL
210 220 230 240 250
ESPGPERVWR ASWGEFQARV PERAPLPPSV PSQFQARMSE NVPLPETHQF
260 270 280 290 300
GEGVSSPKTH LGETLTPLSK AYQSLSAPFP KVRRLEGSFL GGSEAGERLL
310 320 330 340 350
QQGLAQVEAG RRQAEATRQA AAQEERLADL ASDLLLQYLL QGGARQRDLG
360 370 380 390 400
GRGLQETQQE RENEREEEAE QERRGGGEDE VGEEDEEAAE AEAEAEEAER
410 420 430 440 450
ARQNALLFAE EEDGEAGAED KRSQEEAPGH RRKDAEGTEE GGEEDDDDEE
460 470 480 490 500
MDPQTIDSLI ELSTKLHLPA DDVVSIIEEV EEKRKRKKNA PPEPVPPPRA
510 520 530 540 550
APAPTHVRSP QPPPPAPARD ELPDWNEVLP PWDREEDEVF PPGPYHPFPN
560 570 580 590 600
YIRPRTLQPP ASSRRRHFHH ALPPARHHPD LEAQARRAQE EADAEERRLQ
610
EQEELENYIE HVLLHRP
Length:617
Mass (Da):68,179
Last modified:May 26, 2009 - v3
Checksum:i5E23E47DF460C7B4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti342 – 3421G → A (PubMed:2676516).Curated

Mass spectrometryi

Molecular mass is 4164.694 Da from positions 24 - 63. Determined by ESI. 1 Publication
Molecular mass is 1655.730 Da from positions 180 - 194. Determined by ESI. 1 Publication
Molecular mass is 3470.868 Da from positions 375 - 407. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60522 Genomic DNA. Translation: AAA41700.1.
M60525 mRNA. Translation: AAA86428.1.
M74223 mRNA. Translation: AAA42336.1.
PIRiI56530.
S05381.
RefSeqiNP_112259.1. NM_030997.1.
UniGeneiRn.9704.

Genome annotation databases

GeneIDi29461.
KEGGirno:29461.
UCSCiRGD:69399. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60522 Genomic DNA. Translation: AAA41700.1.
M60525 mRNA. Translation: AAA86428.1.
M74223 mRNA. Translation: AAA42336.1.
PIRiI56530.
S05381.
RefSeqiNP_112259.1. NM_030997.1.
UniGeneiRn.9704.

3D structure databases

ProteinModelPortaliP20156.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248104. 1 interaction.
IntActiP20156. 1 interaction.
MINTiMINT-7139146.
STRINGi10116.ENSRNOP00000050024.

Proteomic databases

PaxDbiP20156.
PRIDEiP20156.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29461.
KEGGirno:29461.
UCSCiRGD:69399. rat.

Organism-specific databases

CTDi7425.
RGDi69399. Vgf.

Phylogenomic databases

eggNOGiENOG410IKTZ. Eukaryota.
ENOG410YR85. LUCA.
HOGENOMiHOG000120127.
HOVERGENiHBG006806.
InParanoidiP20156.
PhylomeDBiP20156.

Miscellaneous databases

NextBioi609256.
PROiP20156.

Family and domain databases

InterProiIPR026128. VGF.
[Graphical view]
PANTHERiPTHR15159. PTHR15159. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "A protein induced by NGF in PC12 cells is stored in secretory vesicles and released through the regulated pathway."
    Possenti R., Eldridge J.D., Paterson B.M., Grasso A., Levi A.
    EMBO J. 8:2217-2223(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Possenti R.
    Submitted (OCT-1995) to UniProtKB
    Cited for: SEQUENCE REVISION.
  3. "Structure of the gene encoding VGF, a nervous system-specific mRNA that is rapidly and selectively induced by nerve growth factor in PC12 cells."
    Salton S.R.J., Fischberg D.J., Dong K.-W.
    Mol. Cell. Biol. 11:2335-2349(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  4. "NGF induces the expression of the VGF gene through a cAMP response element."
    Hawley R.J., Scheibe R.J., Wagner J.A.
    J. Neurosci. 12:2573-2581(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "Discovering new bioactive neuropeptides in the striatum secretome using in vivo microdialysis and versatile proteomics."
    Bernay B., Gaillard M.-C., Guryca V., Emadali A., Kuhn L., Bertrand A., Detraz I., Carcenac C., Savasta M., Brouillet E., Garin J., Elalouf J.-M.
    Mol. Cell. Proteomics 8:946-958(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-63; 180-194 AND 375-407, MASS SPECTROMETRY, PYROGLUTAMATE FORMATION AT GLN-180.
    Strain: Wistar.
    Tissue: Corpus striatum.
  6. "In vitro and in vivo pharmacological role of TLQP-21, a VGF-derived peptide, in the regulation of rat gastric motor functions."
    Severini C., La Corte G., Improta G., Broccardo M., Agostini S., Petrella C., Sibilia V., Pagani F., Guidobono F., Bulgarelli I., Ferri G.L., Brancia C., Rinaldi A.M., Levi A., Possenti R.
    Br. J. Pharmacol. 157:984-993(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PEPTIDES, FUNCTION OF PEPTIDE TLQP-21.
  7. "Neuroendocrine regulatory peptide-1 and -2: novel bioactive peptides processed from VGF."
    Toshinai K., Nakazato M.
    Cell. Mol. Life Sci. 66:1939-1945(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF PEPTIDES NERP-1 AND NERP-2, PYROGLUTAMATE FORMATION AT GLN-313, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiVGF_RAT
AccessioniPrimary (citable) accession number: P20156
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: May 26, 2009
Last modified: May 11, 2016
This is version 96 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.