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Protein

Serine protease inhibitor Kazal-type 2

Gene

SPINK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Strong inhibitor of acrosin in male and/or female genital tract. Also inhibits trypsin.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei46 – 47Reactive bond2

GO - Molecular functioni

  • endopeptidase inhibitor activity Source: ProtInc
  • serine-type endopeptidase inhibitor activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Enzyme and pathway databases

BioCyciZFISH:ENSG00000128040-MONOMER.

Protein family/group databases

MEROPSiI01.012.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor Kazal-type 2
Alternative name(s):
Acrosin-trypsin inhibitor
Epididymis tissue protein Li 172
HUSI-II
Gene namesi
Name:SPINK2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:11245. SPINK2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi45P → A: No effect on inhibitory activity towards trypsin. 1 Publication1
Mutagenesisi46R → A: Loss of inhibitory activity towards trypsin. 1 Publication1
Mutagenesisi47H → A: Reduces inhibitory activity towards trypsin. 1 Publication1
Mutagenesisi48F → A: Reduces inhibitory activity towards trypsin. 1 Publication1

Organism-specific databases

DisGeNETi6691.
OpenTargetsiENSG00000128040.
PharmGKBiPA36075.

Polymorphism and mutation databases

DMDMi123985.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000001656124 – 84Serine protease inhibitor Kazal-type 2Add BLAST61

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi36 ↔ 66PROSITE-ProRule annotation1 Publication
Disulfide bondi44 ↔ 63PROSITE-ProRule annotation1 Publication
Disulfide bondi52 ↔ 84PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

EPDiP20155.
PaxDbiP20155.
PeptideAtlasiP20155.
PRIDEiP20155.

PTM databases

iPTMnetiP20155.
PhosphoSitePlusiP20155.

Expressioni

Tissue specificityi

Expressed in epididymis (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000128040.
CleanExiHS_SPINK2.
ExpressionAtlasiP20155. baseline and differential.
GenevisibleiP20155. HS.

Organism-specific databases

HPAiCAB020824.
HPA026813.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ADAMTSL4Q6UY14-33EBI-10200479,EBI-10173507
KRTAP5-9P263713EBI-10200479,EBI-3958099
NOTCH2NLQ7Z3S93EBI-10200479,EBI-945833

Protein-protein interaction databases

BioGridi112569. 26 interactors.
IntActiP20155. 4 interactors.
STRINGi9606.ENSP00000248701.

Structurei

Secondary structure

184
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi27 – 32Combined sources6
Beta strandi51 – 53Combined sources3
Turni62 – 64Combined sources3
Helixi65 – 70Combined sources6
Beta strandi73 – 75Combined sources3
Beta strandi78 – 82Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JXDNMR-A23-84[»]
ProteinModelPortaliP20155.
SMRiP20155.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20155.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 84Kazal-likePROSITE-ProRule annotationAdd BLAST55

Sequence similaritiesi

Contains 1 Kazal-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IP08. Eukaryota.
ENOG410ZF57. LUCA.
GeneTreeiENSGT00530000064285.
HOGENOMiHOG000090244.
InParanoidiP20155.
PhylomeDBiP20155.

Family and domain databases

InterProiIPR002350. Kazal_dom.
[Graphical view]
PfamiPF00050. Kazal_1. 1 hit.
[Graphical view]
SMARTiSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20155-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSVLRLAL LLLAVTFAAS LIPQFGLFSK YRTPNCSQYR LPGCPRHFNP
60 70 80
VCGSDMSTYA NECTLCMKIR EGGHNIKIIR NGPC
Length:84
Mass (Da):9,291
Last modified:December 1, 1992 - v2
Checksum:iE1DE1792BFB1BB85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91438 Genomic DNA. Translation: AAB59431.1.
X57655 mRNA. Translation: CAB37834.1.
M84967 Genomic DNA. No translation available.
GU727622 mRNA. Translation: ADU87624.1.
AK312222 mRNA. Translation: BAG35155.1.
CR542135 mRNA. Translation: CAG46932.1.
CH471057 Genomic DNA. Translation: EAX05513.1.
BC022514 mRNA. Translation: AAH22514.1.
CCDSiCCDS3508.1.
PIRiJU0152.
RefSeqiNP_001258650.1. NM_001271721.1.
NP_001258651.1. NM_001271722.1.
NP_066937.1. NM_021114.3.
UniGeneiHs.98243.

Genome annotation databases

EnsembliENST00000248701; ENSP00000248701; ENSG00000128040.
GeneIDi6691.
KEGGihsa:6691.
UCSCiuc003hcg.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91438 Genomic DNA. Translation: AAB59431.1.
X57655 mRNA. Translation: CAB37834.1.
M84967 Genomic DNA. No translation available.
GU727622 mRNA. Translation: ADU87624.1.
AK312222 mRNA. Translation: BAG35155.1.
CR542135 mRNA. Translation: CAG46932.1.
CH471057 Genomic DNA. Translation: EAX05513.1.
BC022514 mRNA. Translation: AAH22514.1.
CCDSiCCDS3508.1.
PIRiJU0152.
RefSeqiNP_001258650.1. NM_001271721.1.
NP_001258651.1. NM_001271722.1.
NP_066937.1. NM_021114.3.
UniGeneiHs.98243.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JXDNMR-A23-84[»]
ProteinModelPortaliP20155.
SMRiP20155.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112569. 26 interactors.
IntActiP20155. 4 interactors.
STRINGi9606.ENSP00000248701.

Protein family/group databases

MEROPSiI01.012.

PTM databases

iPTMnetiP20155.
PhosphoSitePlusiP20155.

Polymorphism and mutation databases

DMDMi123985.

Proteomic databases

EPDiP20155.
PaxDbiP20155.
PeptideAtlasiP20155.
PRIDEiP20155.

Protocols and materials databases

DNASUi6691.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000248701; ENSP00000248701; ENSG00000128040.
GeneIDi6691.
KEGGihsa:6691.
UCSCiuc003hcg.3. human.

Organism-specific databases

CTDi6691.
DisGeNETi6691.
GeneCardsiSPINK2.
HGNCiHGNC:11245. SPINK2.
HPAiCAB020824.
HPA026813.
MIMi605753. gene.
neXtProtiNX_P20155.
OpenTargetsiENSG00000128040.
PharmGKBiPA36075.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IP08. Eukaryota.
ENOG410ZF57. LUCA.
GeneTreeiENSGT00530000064285.
HOGENOMiHOG000090244.
InParanoidiP20155.
PhylomeDBiP20155.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000128040-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP20155.
GeneWikiiSPINK2.
GenomeRNAii6691.
PROiP20155.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000128040.
CleanExiHS_SPINK2.
ExpressionAtlasiP20155. baseline and differential.
GenevisibleiP20155. HS.

Family and domain databases

InterProiIPR002350. Kazal_dom.
[Graphical view]
PfamiPF00050. Kazal_1. 1 hit.
[Graphical view]
SMARTiSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISK2_HUMAN
AccessioniPrimary (citable) accession number: P20155
Secondary accession number(s): Q6FGH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.