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P20155

- ISK2_HUMAN

UniProt

P20155 - ISK2_HUMAN

Protein

Serine protease inhibitor Kazal-type 2

Gene

SPINK2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Strong inhibitor of acrosin in male and/or female genital tract. Also inhibits trypsin.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei46 – 472Reactive bond

    GO - Molecular functioni

    1. endopeptidase inhibitor activity Source: ProtInc
    2. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

    GO - Biological processi

    1. fertilization Source: Ensembl
    2. male germ cell proliferation Source: Ensembl
    3. negative regulation of serine-type endopeptidase activity Source: Ensembl
    4. seminiferous tubule development Source: Ensembl
    5. spermatid development Source: Ensembl

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Protein family/group databases

    MEROPSiI01.012.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine protease inhibitor Kazal-type 2
    Alternative name(s):
    Acrosin-trypsin inhibitor
    Epididymis tissue protein Li 172
    HUSI-II
    Gene namesi
    Name:SPINK2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:11245. SPINK2.

    Subcellular locationi

    GO - Cellular componenti

    1. acrosomal vesicle Source: Ensembl
    2. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi45 – 451P → A: No effect on inhibitory activity towards trypsin. 1 Publication
    Mutagenesisi46 – 461R → A: Loss of inhibitory activity towards trypsin. 1 Publication
    Mutagenesisi47 – 471H → A: Reduces inhibitory activity towards trypsin. 1 Publication
    Mutagenesisi48 – 481F → A: Reduces inhibitory activity towards trypsin. 1 Publication

    Organism-specific databases

    PharmGKBiPA36075.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23231 PublicationAdd
    BLAST
    Chaini24 – 8461Serine protease inhibitor Kazal-type 2PRO_0000016561Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei24 – 241Pyrrolidone carboxylic acid1 Publication
    Disulfide bondi36 ↔ 661 PublicationPROSITE-ProRule annotation
    Disulfide bondi44 ↔ 631 PublicationPROSITE-ProRule annotation
    Disulfide bondi52 ↔ 841 PublicationPROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Pyrrolidone carboxylic acid

    Proteomic databases

    MaxQBiP20155.
    PaxDbiP20155.
    PRIDEiP20155.

    PTM databases

    PhosphoSiteiP20155.

    Expressioni

    Tissue specificityi

    Expressed in epididymis (at protein level).1 Publication

    Gene expression databases

    ArrayExpressiP20155.
    BgeeiP20155.
    CleanExiHS_SPINK2.
    GenevestigatoriP20155.

    Organism-specific databases

    HPAiCAB020824.
    HPA026813.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000248701.

    Structurei

    Secondary structure

    1
    84
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi27 – 326
    Beta strandi51 – 533
    Turni62 – 643
    Helixi65 – 706
    Beta strandi73 – 753
    Beta strandi78 – 825

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2JXDNMR-A23-84[»]
    ProteinModelPortaliP20155.
    SMRiP20155. Positions 23-84.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP20155.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 8455Kazal-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Kazal-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG255629.
    HOGENOMiHOG000090244.
    InParanoidiP20155.
    OrthoDBiEOG7BW0M2.
    PhylomeDBiP20155.

    Family and domain databases

    InterProiIPR002350. Kazal_dom.
    [Graphical view]
    PfamiPF00050. Kazal_1. 1 hit.
    [Graphical view]
    SMARTiSM00280. KAZAL. 1 hit.
    [Graphical view]
    PROSITEiPS00282. KAZAL_1. 1 hit.
    PS51465. KAZAL_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P20155-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALSVLRLAL LLLAVTFAAS LIPQFGLFSK YRTPNCSQYR LPGCPRHFNP   50
    VCGSDMSTYA NECTLCMKIR EGGHNIKIIR NGPC 84
    Length:84
    Mass (Da):9,291
    Last modified:December 1, 1992 - v2
    Checksum:iE1DE1792BFB1BB85
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91438 Genomic DNA. Translation: AAB59431.1.
    X57655 mRNA. Translation: CAB37834.1.
    M84967 Genomic DNA. No translation available.
    GU727622 mRNA. Translation: ADU87624.1.
    AK312222 mRNA. Translation: BAG35155.1.
    CR542135 mRNA. Translation: CAG46932.1.
    CH471057 Genomic DNA. Translation: EAX05513.1.
    BC022514 mRNA. Translation: AAH22514.1.
    CCDSiCCDS3508.1.
    PIRiJU0152.
    RefSeqiNP_001258650.1. NM_001271721.1.
    NP_001258651.1. NM_001271722.1.
    NP_066937.1. NM_021114.3.
    UniGeneiHs.98243.

    Genome annotation databases

    EnsembliENST00000248701; ENSP00000248701; ENSG00000128040.
    GeneIDi6691.
    KEGGihsa:6691.
    UCSCiuc003hcg.2. human.

    Polymorphism databases

    DMDMi123985.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91438 Genomic DNA. Translation: AAB59431.1 .
    X57655 mRNA. Translation: CAB37834.1 .
    M84967 Genomic DNA. No translation available.
    GU727622 mRNA. Translation: ADU87624.1 .
    AK312222 mRNA. Translation: BAG35155.1 .
    CR542135 mRNA. Translation: CAG46932.1 .
    CH471057 Genomic DNA. Translation: EAX05513.1 .
    BC022514 mRNA. Translation: AAH22514.1 .
    CCDSi CCDS3508.1.
    PIRi JU0152.
    RefSeqi NP_001258650.1. NM_001271721.1.
    NP_001258651.1. NM_001271722.1.
    NP_066937.1. NM_021114.3.
    UniGenei Hs.98243.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2JXD NMR - A 23-84 [» ]
    ProteinModelPortali P20155.
    SMRi P20155. Positions 23-84.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000248701.

    Protein family/group databases

    MEROPSi I01.012.

    PTM databases

    PhosphoSitei P20155.

    Polymorphism databases

    DMDMi 123985.

    Proteomic databases

    MaxQBi P20155.
    PaxDbi P20155.
    PRIDEi P20155.

    Protocols and materials databases

    DNASUi 6691.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000248701 ; ENSP00000248701 ; ENSG00000128040 .
    GeneIDi 6691.
    KEGGi hsa:6691.
    UCSCi uc003hcg.2. human.

    Organism-specific databases

    CTDi 6691.
    GeneCardsi GC04M057676.
    HGNCi HGNC:11245. SPINK2.
    HPAi CAB020824.
    HPA026813.
    MIMi 605753. gene.
    neXtProti NX_P20155.
    PharmGKBi PA36075.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG255629.
    HOGENOMi HOG000090244.
    InParanoidi P20155.
    OrthoDBi EOG7BW0M2.
    PhylomeDBi P20155.

    Miscellaneous databases

    EvolutionaryTracei P20155.
    GeneWikii SPINK2.
    GenomeRNAii 6691.
    NextBioi 26077.
    PROi P20155.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P20155.
    Bgeei P20155.
    CleanExi HS_SPINK2.
    Genevestigatori P20155.

    Family and domain databases

    InterProi IPR002350. Kazal_dom.
    [Graphical view ]
    Pfami PF00050. Kazal_1. 1 hit.
    [Graphical view ]
    SMARTi SM00280. KAZAL. 1 hit.
    [Graphical view ]
    PROSITEi PS00282. KAZAL_1. 1 hit.
    PS51465. KAZAL_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and sequence analysis of the cDNA encoding the human acrosin-trypsin inhibitor (HUSI-II)."
      Moeritz A., Lilja H., Fink E.
      FEBS Lett. 278:127-130(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Organization and sequence of the gene encoding the human acrosin-trypsin inhibitor (HUSI-II)."
      Moeritz A., Grzeschik K.H., Wingender E., Fink E.
      Gene 123:277-281(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins."
      Li J., Liu F., Wang H., Liu X., Liu J., Li N., Wan F., Wang W., Zhang C., Jin S., Liu J., Zhu P., Liu Y.
      Mol. Cell. Proteomics 9:2517-2528(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Epididymis.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    5. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    8. "Amino acid sequence elucidation of human acrosin-trypsin inhibitor (HUSI-II) reveals that Kazal-type proteinase inhibitors are structurally related to beta-subunits of glycoprotein hormones."
      Fink E., Hehlein-Fink C., Eulitz M.
      FEBS Lett. 270:222-224(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-84, PYROGLUTAMATE FORMATION AT GLN-24.
      Tissue: Semen.
    9. "Identification of trypsin-inhibitory site and structure determination of human SPINK2 serine proteinase inhibitor."
      Chen T., Lee T.-R., Liang W.-G., Chang W.-S., Lyu P.-C.
      Proteins 77:209-219(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 23-84, FUNCTION, MUTAGENESIS OF PRO-45; ARG-46; HIS-47 AND PHE-48, IDENTIFICATION BY MASS SPECTROMETRY, DISULFIDE BONDS.

    Entry informationi

    Entry nameiISK2_HUMAN
    AccessioniPrimary (citable) accession number: P20155
    Secondary accession number(s): Q6FGH2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3