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P20155 (ISK2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine protease inhibitor Kazal-type 2
Alternative name(s):
Acrosin-trypsin inhibitor
Epididymis tissue protein Li 172
HUSI-II
Gene names
Name:SPINK2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length84 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Strong inhibitor of acrosin in male and/or female genital tract. Also inhibits trypsin. Ref.9

Subcellular location

Secreted.

Tissue specificity

Expressed in epididymis (at protein level). Ref.3

Sequence similarities

Contains 1 Kazal-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.8
Chain24 – 8461Serine protease inhibitor Kazal-type 2
PRO_0000016561

Regions

Domain30 – 8455Kazal-like

Sites

Site46 – 472Reactive bond

Amino acid modifications

Modified residue241Pyrrolidone carboxylic acid Ref.8
Disulfide bond36 ↔ 66 Ref.9
Disulfide bond44 ↔ 63 Ref.9
Disulfide bond52 ↔ 84 Ref.9

Experimental info

Mutagenesis451P → A: No effect on inhibitory activity towards trypsin. Ref.9
Mutagenesis461R → A: Loss of inhibitory activity towards trypsin. Ref.9
Mutagenesis471H → A: Reduces inhibitory activity towards trypsin. Ref.9
Mutagenesis481F → A: Reduces inhibitory activity towards trypsin. Ref.9

Secondary structure

............ 84
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P20155 [UniParc].

Last modified December 1, 1992. Version 2.
Checksum: E1DE1792BFB1BB85

FASTA849,291
        10         20         30         40         50         60 
MALSVLRLAL LLLAVTFAAS LIPQFGLFSK YRTPNCSQYR LPGCPRHFNP VCGSDMSTYA 

        70         80 
NECTLCMKIR EGGHNIKIIR NGPC 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and sequence analysis of the cDNA encoding the human acrosin-trypsin inhibitor (HUSI-II)."
Moeritz A., Lilja H., Fink E.
FEBS Lett. 278:127-130(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Organization and sequence of the gene encoding the human acrosin-trypsin inhibitor (HUSI-II)."
Moeritz A., Grzeschik K.H., Wingender E., Fink E.
Gene 123:277-281(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins."
Li J., Liu F., Wang H., Liu X., Liu J., Li N., Wan F., Wang W., Zhang C., Jin S., Liu J., Zhu P., Liu Y.
Mol. Cell. Proteomics 9:2517-2528(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Epididymis.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[5]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[8]"Amino acid sequence elucidation of human acrosin-trypsin inhibitor (HUSI-II) reveals that Kazal-type proteinase inhibitors are structurally related to beta-subunits of glycoprotein hormones."
Fink E., Hehlein-Fink C., Eulitz M.
FEBS Lett. 270:222-224(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-84, PYROGLUTAMATE FORMATION AT GLN-24.
Tissue: Semen.
[9]"Identification of trypsin-inhibitory site and structure determination of human SPINK2 serine proteinase inhibitor."
Chen T., Lee T.-R., Liang W.-G., Chang W.-S., Lyu P.-C.
Proteins 77:209-219(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 23-84, FUNCTION, MUTAGENESIS OF PRO-45; ARG-46; HIS-47 AND PHE-48, IDENTIFICATION BY MASS SPECTROMETRY, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M91438 Genomic DNA. Translation: AAB59431.1.
X57655 mRNA. Translation: CAB37834.1.
M84967 Genomic DNA. No translation available.
GU727622 mRNA. Translation: ADU87624.1.
AK312222 mRNA. Translation: BAG35155.1.
CR542135 mRNA. Translation: CAG46932.1.
CH471057 Genomic DNA. Translation: EAX05513.1.
BC022514 mRNA. Translation: AAH22514.1.
CCDSCCDS3508.1.
PIRJU0152.
RefSeqNP_001258650.1. NM_001271721.1.
NP_001258651.1. NM_001271722.1.
NP_066937.1. NM_021114.3.
UniGeneHs.98243.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2JXDNMR-A23-84[»]
ProteinModelPortalP20155.
SMRP20155. Positions 23-84.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000248701.

Protein family/group databases

MEROPSI01.012.

PTM databases

PhosphoSiteP20155.

Polymorphism databases

DMDM123985.

Proteomic databases

MaxQBP20155.
PaxDbP20155.
PRIDEP20155.

Protocols and materials databases

DNASU6691.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000248701; ENSP00000248701; ENSG00000128040.
GeneID6691.
KEGGhsa:6691.
UCSCuc003hcg.2. human.

Organism-specific databases

CTD6691.
GeneCardsGC04M057676.
HGNCHGNC:11245. SPINK2.
HPACAB020824.
HPA026813.
MIM605753. gene.
neXtProtNX_P20155.
PharmGKBPA36075.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG255629.
HOGENOMHOG000090244.
InParanoidP20155.
OrthoDBEOG7BW0M2.
PhylomeDBP20155.

Gene expression databases

ArrayExpressP20155.
BgeeP20155.
CleanExHS_SPINK2.
GenevestigatorP20155.

Family and domain databases

InterProIPR002350. Kazal_dom.
[Graphical view]
PfamPF00050. Kazal_1. 1 hit.
[Graphical view]
SMARTSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP20155.
GeneWikiSPINK2.
GenomeRNAi6691.
NextBio26077.
PROP20155.
SOURCESearch...

Entry information

Entry nameISK2_HUMAN
AccessionPrimary (citable) accession number: P20155
Secondary accession number(s): Q6FGH2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 1, 1992
Last modified: July 9, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM