Reviewed, UniProtKB/Swiss-Prot P20151 (KLK2_HUMAN)

Names and origin

Protein names

Recommended name:
    Kallikrein-2
    EC=3.4.21.35
Alternative name(s):
    Tissue kallikrein-2
    Glandular kallikrein-1
      Short name=hGK-1

Gene names

Name:

KLK2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P20151-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P20151-2)

Also known as: PGK-10A;

The sequence of this isoform differs from the canonical sequence as follows:
     211-261: GDSGGPLVCNGVLQGITSWGPEPCALPEKPAVYTKVVHYRKWIKDTIAANP → VSHPYSQHLEGK

Isoform 3 (identifier: P20151-3)

The sequence of this isoform differs from the canonical sequence as follows:
     165-261: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Probable
	Propeptide	19 – 24	6	Activation peptide Probable		PRO_0000027929
	Chain	25 – 261	237	Kallikrein-2		PRO_0000027930
Regions
	Domain	25 – 258	234	Peptidase S1
Sites
	Active site	65	1	Charge relay system
	Active site	120	1	Charge relay system
	Active site	213	1	Charge relay system
Amino acid modifications
	Glycosylation	102	1	N-linked (GlcNAc...) Potential
	Disulfide bond	31 ↔ 173		By similarity
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	152 ↔ 219		By similarity
	Disulfide bond	184 ↔ 198		By similarity
	Disulfide bond	209 ↔ 234		By similarity
Natural variations
	Alternative sequence	165 – 261	97	Missing in isoform 3.		VSP_005400
	Alternative sequence	211 – 261	51	GDSGG…IAANP → VSHPYSQHLEGK in isoform 2.		VSP_005399
	Natural variant	18	1	V → L: dbSNP rs6072. Ref.9		VAR_014164
	Natural variant	250	1	R → W: dbSNP rs198977.		VAR_020178

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 9CF7F4A1162EF42D	FASTA	261	28,671
	10 20 30 40 50 60 MWDLVLSIAL SVGCTGAVPL IQSRIVGGWE CEKHSQPWQV AVYSHGWAHC GGVLVHPQWV 70 80 90 100 110 120 LTAAHCLKKN SQVWLGRHNL FEPEDTGQRV PVSHSFPHPL YNMSLLKHQS LRPDEDSSHD 130 140 150 160 170 180 LMLLRLSEPA KITDVVKVLG LPTQEPALGT TCYASGWGSI EPEEFLRPRS LQCVSLHLLS 190 200 210 220 230 240 NDMCARAYSE KVTEFMLCAG LWTGGKDTCG GDSGGPLVCN GVLQGITSWG PEPCALPEKP 250 260 AVYTKVVHYR KWIKDTIAAN P
	Isoform 2 (PGK-10A). Checksum: BF76900C2AFEA941	FASTA	222	24,617
	10 20 30 40 50 60 MWDLVLSIAL SVGCTGAVPL IQSRIVGGWE CEKHSQPWQV AVYSHGWAHC GGVLVHPQWV 70 80 90 100 110 120 LTAAHCLKKN SQVWLGRHNL FEPEDTGQRV PVSHSFPHPL YNMSLLKHQS LRPDEDSSHD 130 140 150 160 170 180 LMLLRLSEPA KITDVVKVLG LPTQEPALGT TCYASGWGSI EPEEFLRPRS LQCVSLHLLS 190 200 210 220 NDMCARAYSE KVTEFMLCAG LWTGGKDTCG VSHPYSQHLE GK
	Isoform 3. Checksum: 00D2AE5561DB788E	FASTA	164	18,162
	10 20 30 40 50 60 MWDLVLSIAL SVGCTGAVPL IQSRIVGGWE CEKHSQPWQV AVYSHGWAHC GGVLVHPQWV 70 80 90 100 110 120 LTAAHCLKKN SQVWLGRHNL FEPEDTGQRV PVSHSFPHPL YNMSLLKHQS LRPDEDSSHD 130 140 150 160 LMLLRLSEPA KITDVVKVLG LPTQEPALGT TCYASGWGSI EPEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of a human glandular kallikrein gene." Schedlich L.J., Bennetts B.H., Morris B.J. DNA 6:429-437(1987) [PubMed: 2824146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[2]	"Identification and androgen-regulated expression of two major human glandular kallikrein-1 (hGK-1) mRNA species." Riegman P.H., Vlietstra R.J., der Korput H.A., Romijn J.C., Trapman J. Mol. Cell. Endocrinol. 76:181-190(1991) [PubMed: 1726490] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Prostate.
[3]	"Identification of three new alternate human kallikrein 2 transcripts: evidence of long transcript and alternative splicing." Liu X.F., Essand M., Vasmatzis G., Lee B., Pastan I. Biochem. Biophys. Res. Commun. 264:833-839(1999) [PubMed: 10544017] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
[4]	"Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region." Gan L., Lee I., Smith R., Argonza-Barrett R., Lei H., McCuaig J., Moss P., Paeper B., Wang K. Gene 257:119-130(2000) [PubMed: 11054574] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Prostate.
[8]	"An unappreciated role for RNA surveillance." Hillman R.T., Green R.E., Brenner S.E. Genome Biol. 5:RESEARCH008.1-RESEARCH008.16(2004) [PubMed: 14759258] [Abstract] Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
[9]	"Characterization of single-nucleotide polymorphisms in coding regions of human genes." Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S. Nat. Genet. 22:231-238(1999) [PubMed: 10391209] [Abstract] Cited for: VARIANT LEU-18.
[10]	Erratum Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S. Nat. Genet. 23:373-373(1999)
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M18156 Genomic DNA. No translation available. M18157 Genomic DNA. Translation: AAA74454.1. S39329 mRNA. Translation: AAD13816.1. S39329 mRNA. Translation: AAD13817.1. AF188745 mRNA. Translation: AAF08275.1. AF188746 mRNA. Translation: AAF08276.1. AF188747 mRNA. Translation: AAF08277.1. AF243527 Genomic DNA. Translation: AAG33356.1. BT006650 mRNA. Translation: AAP35296.1. AC037199 Genomic DNA. No translation available. BC005196 mRNA. Translation: AAH05196.1.
IPI	IPI00022227. IPI00219231. IPI00434943.
PIR	A29586.
RefSeq	NP_001002231.1. NP_005542.1.
UniGene	Hs.515560
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
ModBase	Search...
Protein family/group databases
MEROPS	S01.161.
PTM databases
PhosphoSite	P20151.
Proteomic databases
PRIDE	P20151.
Genome annotation databases
Ensembl	ENSG00000167751. Homo sapiens. [Contig view]
GeneID	3817.
KEGG	hsa:3817.
UCSC	uc002ptu.1. human. uc002ptv.1. human.
Organism-specific databases
GeneCards	GC19P056068.
H-InvDB	HIX0015370.
HGNC	HGNC:6363. KLK2.
HPA	HPA000764.
MIM	147960. gene.
PharmGKB	PA30152.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P20151.
HOVERGEN	P20151.
OMA	P20151. AYSEKVT.
Enzyme and pathway databases
BRENDA	3.4.21.35. 247.
Pathway_Interaction_DB	ar_pathway. Coregulation of Androgen receptor activity. ar_tf_pathway. Regulation of Androgen receptor activity.
Gene expression databases
ArrayExpress	P20151.
Bgee	P20151.
CleanEx	HS_KLK2.
GermOnline	ENSG00000167751. Homo sapiens.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	15005.
PMAP-CutDB	P20151.
SOURCE	Search...

Entry information

Entry name

KLK2_HUMAN

Accession

Primary (citable) accession number: P20151
Secondary accession number(s): Q15946, Q9UJZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	July 7, 2009
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)