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Protein

Gastricsin

Gene

PGC

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes a variety of proteins.

Catalytic activityi

More restricted specificity than pepsin A, but shows preferential cleavage at Tyr-|-Xaa bonds. High activity on hemoglobin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei911
Active sitei2761

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: UniProtKB

GO - Biological processi

  • digestion Source: ProtInc
  • positive regulation of antibacterial peptide production Source: UniProtKB
  • protein catabolic process Source: GO_Central
  • proteolysis Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Biological processi

Digestion

Enzyme and pathway databases

BioCyciZFISH:HS01847-MONOMER.
BRENDAi3.4.23.3. 2681.

Protein family/group databases

MEROPSiA01.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Gastricsin (EC:3.4.23.3)
Alternative name(s):
Pepsinogen C
Gene namesi
Name:PGC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:8890. PGC.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi5225.
OpenTargetsiENSG00000096088.
PharmGKBiPA33228.

Chemistry databases

ChEMBLiCHEMBL2136.
GuidetoPHARMACOLOGYi2391.

Polymorphism and mutation databases

BioMutaiPGC.
DMDMi129796.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 162 PublicationsAdd BLAST16
PropeptideiPRO_000002605617 – 59Activation peptideAdd BLAST43
ChainiPRO_000002605760 – 388GastricsinAdd BLAST329

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi104 ↔ 109
Disulfide bondi267 ↔ 271
Disulfide bondi310 ↔ 343

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

MaxQBiP20142.
PaxDbiP20142.
PeptideAtlasiP20142.
PRIDEiP20142.

PTM databases

iPTMnetiP20142.
PhosphoSitePlusiP20142.

Miscellaneous databases

PMAP-CutDBP20142.

Expressioni

Gene expression databases

BgeeiENSG00000096088.
CleanExiHS_PGC.
ExpressionAtlasiP20142. baseline and differential.
GenevisibleiP20142. HS.

Organism-specific databases

HPAiHPA031717.
HPA031718.

Interactioni

Protein-protein interaction databases

BioGridi111246. 4 interactors.
STRINGi9606.ENSP00000362116.

Chemistry databases

BindingDBiP20142.

Structurei

Secondary structure

1388
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi19 – 25Combined sources7
Helixi29 – 35Combined sources7
Helixi39 – 43Combined sources5
Helixi50 – 54Combined sources5
Helixi65 – 68Combined sources4
Beta strandi73 – 79Combined sources7
Turni80 – 83Combined sources4
Beta strandi84 – 91Combined sources8
Beta strandi97 – 101Combined sources5
Helixi107 – 110Combined sources4
Helixi117 – 119Combined sources3
Beta strandi124 – 134Combined sources11
Beta strandi137 – 150Combined sources14
Beta strandi153 – 165Combined sources13
Helixi169 – 173Combined sources5
Beta strandi178 – 181Combined sources4
Helixi185 – 187Combined sources3
Helixi189 – 191Combined sources3
Helixi195 – 201Combined sources7
Beta strandi205 – 214Combined sources10
Beta strandi219 – 229Combined sources11
Helixi232 – 234Combined sources3
Beta strandi235 – 244Combined sources10
Beta strandi246 – 249Combined sources4
Beta strandi251 – 254Combined sources4
Beta strandi256 – 259Combined sources4
Turni266 – 269Combined sources4
Beta strandi271 – 275Combined sources5
Beta strandi281 – 285Combined sources5
Helixi286 – 288Combined sources3
Helixi289 – 296Combined sources8
Beta strandi306 – 308Combined sources3
Helixi310 – 315Combined sources6
Beta strandi319 – 323Combined sources5
Beta strandi326 – 330Combined sources5
Helixi332 – 335Combined sources4
Beta strandi336 – 338Combined sources3
Beta strandi343 – 350Combined sources8
Beta strandi360 – 363Combined sources4
Helixi365 – 368Combined sources4
Beta strandi371 – 376Combined sources6
Turni377 – 380Combined sources4
Beta strandi381 – 387Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AVFX-ray2.36A/J60-388[»]
P/Q17-42[»]
1HTRX-ray1.62B60-388[»]
P17-59[»]
ProteinModelPortaliP20142.
SMRiP20142.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20142.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 385Peptidase A1PROSITE-ProRule annotationAdd BLAST313

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiP20142.
KOiK01377.
OMAiLSNNGYC.
OrthoDBiEOG091G0JP7.
PhylomeDBiP20142.
TreeFamiTF314990.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033735. Gastricsin.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PTHR13683:SF292. PTHR13683:SF292. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P20142-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKWMVVVLVC LQLLEAAVVK VPLKKFKSIR ETMKEKGLLG EFLRTHKYDP
60 70 80 90 100
AWKYRFGDLS VTYEPMAYMD AAYFGEISIG TPPQNFLVLF DTGSSNLWVP
110 120 130 140 150
SVYCQSQACT SHSRFNPSES STYSTNGQTF SLQYGSGSLT GFFGYDTLTV
160 170 180 190 200
QSIQVPNQEF GLSENEPGTN FVYAQFDGIM GLAYPALSVD EATTAMQGMV
210 220 230 240 250
QEGALTSPVF SVYLSNQQGS SGGAVVFGGV DSSLYTGQIY WAPVTQELYW
260 270 280 290 300
QIGIEEFLIG GQASGWCSEG CQAIVDTGTS LLTVPQQYMS ALLQATGAQE
310 320 330 340 350
DEYGQFLVNC NSIQNLPSLT FIINGVEFPL PPSSYILSNN GYCTVGVEPT
360 370 380
YLSSQNGQPL WILGDVFLRS YYSVYDLGNN RVGFATAA
Length:388
Mass (Da):42,426
Last modified:February 1, 1991 - v1
Checksum:iF862DFDC1438BB92
GO
Isoform 2 (identifier: P20142-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     217-388: QQGSSGGAVV...NNRVGFATAA → LVLESSGLGP...PPRSQHHSGA

Show »
Length:315
Mass (Da):34,208
Checksum:i79BD7C79ABDF8879
GO

Sequence cautioni

The sequence AAA60062 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40 – 41GE → ED AA sequence (PubMed:6816595).Curated2
Sequence conflicti52W → S AA sequence (PubMed:6816595).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042312217 – 388QQGSS…FATAA → LVLESSGLGPLLTPSRAAPP SSTLQLPEKPLEQTWNILTP FTKTLPVSNLSRKVTSWAGV GIPVTCLPEAGSGGERRAEC GLGVPTTRGPPRSQHHSGA in isoform 2. 1 PublicationAdd BLAST172

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18667
, M18659, M18660, M18661, M18662, M18663, M18664, M18665, M18666 Genomic DNA. Translation: AAA60062.1. Different initiation.
J04443 mRNA. Translation: AAA60074.1.
M23077
, M23069, M23070, M23071, M23072, M23073, M23074, M23075 Genomic DNA. Translation: AAA60063.1.
U75272 mRNA. Translation: AAB18273.1.
AK301298 mRNA. Translation: BAG62855.1.
AL365205 Genomic DNA. Translation: CAI13181.1.
BC073740 mRNA. Translation: AAH73740.1.
CCDSiCCDS4859.1. [P20142-1]
CCDS55000.1. [P20142-2]
PIRiA29937.
RefSeqiNP_001159896.1. NM_001166424.1. [P20142-2]
NP_002621.1. NM_002630.3. [P20142-1]
UniGeneiHs.1867.

Genome annotation databases

EnsembliENST00000373025; ENSP00000362116; ENSG00000096088. [P20142-1]
ENST00000425343; ENSP00000405094; ENSG00000096088. [P20142-2]
GeneIDi5225.
KEGGihsa:5225.
UCSCiuc003ora.3. human. [P20142-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18667
, M18659, M18660, M18661, M18662, M18663, M18664, M18665, M18666 Genomic DNA. Translation: AAA60062.1. Different initiation.
J04443 mRNA. Translation: AAA60074.1.
M23077
, M23069, M23070, M23071, M23072, M23073, M23074, M23075 Genomic DNA. Translation: AAA60063.1.
U75272 mRNA. Translation: AAB18273.1.
AK301298 mRNA. Translation: BAG62855.1.
AL365205 Genomic DNA. Translation: CAI13181.1.
BC073740 mRNA. Translation: AAH73740.1.
CCDSiCCDS4859.1. [P20142-1]
CCDS55000.1. [P20142-2]
PIRiA29937.
RefSeqiNP_001159896.1. NM_001166424.1. [P20142-2]
NP_002621.1. NM_002630.3. [P20142-1]
UniGeneiHs.1867.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AVFX-ray2.36A/J60-388[»]
P/Q17-42[»]
1HTRX-ray1.62B60-388[»]
P17-59[»]
ProteinModelPortaliP20142.
SMRiP20142.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111246. 4 interactors.
STRINGi9606.ENSP00000362116.

Chemistry databases

BindingDBiP20142.
ChEMBLiCHEMBL2136.
GuidetoPHARMACOLOGYi2391.

Protein family/group databases

MEROPSiA01.003.

PTM databases

iPTMnetiP20142.
PhosphoSitePlusiP20142.

Polymorphism and mutation databases

BioMutaiPGC.
DMDMi129796.

Proteomic databases

MaxQBiP20142.
PaxDbiP20142.
PeptideAtlasiP20142.
PRIDEiP20142.

Protocols and materials databases

DNASUi5225.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373025; ENSP00000362116; ENSG00000096088. [P20142-1]
ENST00000425343; ENSP00000405094; ENSG00000096088. [P20142-2]
GeneIDi5225.
KEGGihsa:5225.
UCSCiuc003ora.3. human. [P20142-1]

Organism-specific databases

CTDi5225.
DisGeNETi5225.
GeneCardsiPGC.
HGNCiHGNC:8890. PGC.
HPAiHPA031717.
HPA031718.
MIMi169740. gene.
neXtProtiNX_P20142.
OpenTargetsiENSG00000096088.
PharmGKBiPA33228.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiP20142.
KOiK01377.
OMAiLSNNGYC.
OrthoDBiEOG091G0JP7.
PhylomeDBiP20142.
TreeFamiTF314990.

Enzyme and pathway databases

BioCyciZFISH:HS01847-MONOMER.
BRENDAi3.4.23.3. 2681.

Miscellaneous databases

ChiTaRSiPGC. human.
EvolutionaryTraceiP20142.
GeneWikiiGastricsin.
GenomeRNAii5225.
PMAP-CutDBP20142.
PROiP20142.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000096088.
CleanExiHS_PGC.
ExpressionAtlasiP20142. baseline and differential.
GenevisibleiP20142. HS.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033735. Gastricsin.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PTHR13683:SF292. PTHR13683:SF292. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEPC_HUMAN
AccessioniPrimary (citable) accession number: P20142
Secondary accession number(s): B4DVZ3, Q5T3D7, Q5T3D8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 30, 2016
This is version 167 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.