Reviewed, UniProtKB/Swiss-Prot P20133 (PGTB2_YEAST)

Names and origin

Protein names

Recommended name:
    Geranylgeranyl transferase type-2 subunit beta
    EC=2.5.1.60
Alternative name(s):
    Geranylgeranyl transferase type II subunit beta
      Short name=GGTase-II-beta
    Type II protein geranyl-geranyltransferase subunit beta
      Short name=PGGT
    YPT1/SEC4 proteins geranylgeranyltransferase subunit beta

Gene names

Name:	BET2
Ordered Locus Names:	YPR176C
ORF Names:	P9705.12

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to proteins having the C-terminal -XCC or -XCXC, where both cysteines may become modified. Acts on YPT1 and SEC4.
Catalytic activity	Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Heterodimer of an alpha and a beta subunit.
Miscellaneous	Present with 1520 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the protein prenyltransferase subunit beta family. Contains 6 PFTB repeats.

Ontologies

Keywords
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Prenyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid geranylgeranylation Inferred from direct assay. Source: SGD
Cellular component	Rab-protein geranylgeranyltransferase complex Inferred from direct assay. Source: SGD
Molecular function	Rab geranylgeranyltransferase activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BET4	Q00618	1	EBI-3559,EBI-3573

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Geranylgeranyl transferase type-2 subunit beta		PRO_0000119777
Regions
	Repeat	9 – 50	42	PFTB 1
	Repeat	57 – 99	43	PFTB 2
	Repeat	109 – 150	42	PFTB 3
	Repeat	157 – 198	42	PFTB 4
	Repeat	208 – 249	42	PFTB 5
	Repeat	256 – 298	43	PFTB 6
Sites
	Metal binding	234	1	Zinc By similarity
	Metal binding	236	1	Zinc By similarity
	Metal binding	286	1	Zinc By similarity
Experimental info
	Sequence conflict	22	1	K → N Ref.1
	Sequence conflict	22	1	K → N Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P20133-1 [UniParc]. Last modified February 1, 1996. Version 3. Checksum: 6A9C44DFBE62AAFC	FASTA	325	36,666
	10 20 30 40 50 60 MSGSLTLLKE KHIRYIESLD TKKHNFEYWL TEHLRLNGIY WGLTALCVLD SPETFVKEEV 70 80 90 100 110 120 ISFVLSCWDD KYGAFAPFPR HDAHLLTTLS AVQILATYDA LDVLGKDRKV RLISFIRGNQ 130 140 150 160 170 180 LEDGSFQGDR FGEVDTRFVY TALSALSILG ELTSEVVDPA VDFVLKCYNF DGGFGLCPNA 190 200 210 220 230 240 ESHAAQAFTC LGALAIANKL DMLSDDQLEE IGWWLCERQL PEGGLNGRPS KLPDVCYSWW 250 260 270 280 290 300 VLSSLAIIGR LDWINYEKLT EFILKCQDEK KGGISDRPEN EVDVFHTVFG VAGLSLMGYD 310 320 NLVPIDPIYC MPKSVTSKFK KYPYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An essential gene in Saccharomyces cerevisiae shares an upstream regulatory element with PRP4." Petersen-Bjoern S., Harrington T.R., Friesen J.D. Yeast 6:345-352(1990) [PubMed: 2204247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Dependence of Ypt1 and Sec4 membrane attachment on Bet2." Rossi G., Jiang Y., Newman A.P., Ferro-Novick S. Nature 351:158-161(1991) [PubMed: 1903184] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle." Petersen-Bjoern S., Soltyk A., Beggs J.D., Friesen J.D. Mol. Cell. Biol. 9:3698-3709(1989) [PubMed: 2528686] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-187.
[6]	"Bet2p and Mad2p are components of a prenyltransferase that adds geranylgeranyl onto Ypt1p and Sec4p." Jiang Y., Rossi G., Ferro-Novick S. Nature 366:84-86(1993) [PubMed: 8232542] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M29585 Genomic DNA. Translation: AAA66939.1. M26597 Genomic DNA. Translation: AAA79331.1. U25842 Genomic DNA. Translation: AAB68110.1. AY558067 Genomic DNA. Translation: AAS56393.1.
PIR	S59834.
RefSeq	NP_015502.1.
3D structure databases
HSSP	HSSP built from PDB template 1DCE based on UniProtKB Q08603.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2214N.
IntAct	P20133. 5 interactions.
STRING	P20133.
Genome annotation databases
Ensembl	YPR176C; YPR176C; YPR176C; Saccharomyces cerevisiae. [Genome view]
GeneID	856306.
GenomeReviews	Gene locus YPR176C in contig U00094_GR.
KEGG	sce:YPR176C.
NMPDR	fig|4932.3.peg.6651.
Organism-specific databases
CYGD	YPR176c.
SGD	S000006380. BET2.
Phylogenomic databases
HOGENOM	P20133.
OMA	YWGLTAL.
Enzyme and pathway databases
BRENDA	2.5.1.60. 250.
Gene expression databases
ArrayExpress	P20133.
Genevestigator	P20133.
GermOnline	YPR176C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001330. Prenyltrans. [Graphical view]
Pfam	PF00432. Prenyltrans. 5 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981673.

Entry information

Entry name

PGTB2_YEAST

Accession

Primary (citable) accession number: P20133
Secondary accession number(s): P32433

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)