P20133 (PGTB2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Geranylgeranyl transferase type-2 subunit beta
EC=2.5.1.60

Alternative name(s):
Geranylgeranyl transferase type II subunit beta
Short name=GGTase-II-beta
Type II protein geranyl-geranyltransferase subunit beta
Short name=PGGT
YPT1/SEC4 proteins geranylgeranyltransferase subunit beta

Gene names

Name:	BET2
Ordered Locus Names:	YPR176C
ORF Names:	P9705.12

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to proteins having the C-terminal -XCC or -XCXC, where both cysteines may become modified. Acts on YPT1 and SEC4.
Catalytic activity	Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Heterodimer of an alpha and a beta subunit.
Miscellaneous	Present with 1520 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the protein prenyltransferase subunit beta family. Contains 6 PFTB repeats.

Ontologies

Keywords
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Prenyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from mutant phenotype PubMed 3312234PubMed 8232541. Source: SGD protein geranylgeranylation Inferred from direct assay PubMed 8756702. Source: SGD protein targeting to membrane Inferred from mutant phenotype Ref.2. Source: SGD
Cellular_component	Rab-protein geranylgeranyltransferase complex Inferred from direct assay PubMed 8183917PubMed 8583924PubMed 8756702. Source: SGD
Molecular_function	Rab geranylgeranyltransferase activity Inferred from direct assay PubMed 8756702. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BET4	Q00618	3	EBI-3559,EBI-3573

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Geranylgeranyl transferase type-2 subunit beta		PRO_0000119777
Regions
	Repeat	9 – 50	42	PFTB 1
	Repeat	57 – 99	43	PFTB 2
	Repeat	109 – 150	42	PFTB 3
	Repeat	157 – 198	42	PFTB 4
	Repeat	208 – 249	42	PFTB 5
	Repeat	256 – 298	43	PFTB 6
Sites
	Metal binding	234	1	Zinc By similarity
	Metal binding	236	1	Zinc By similarity
	Metal binding	286	1	Zinc By similarity
Experimental info
	Sequence conflict	22	1	K → N Ref.1
	Sequence conflict	22	1	K → N Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P20133 [UniParc]. Last modified February 1, 1996. Version 3. Checksum: 6A9C44DFBE62AAFC	FASTA	325	36,666
	10 20 30 40 50 60 MSGSLTLLKE KHIRYIESLD TKKHNFEYWL TEHLRLNGIY WGLTALCVLD SPETFVKEEV 70 80 90 100 110 120 ISFVLSCWDD KYGAFAPFPR HDAHLLTTLS AVQILATYDA LDVLGKDRKV RLISFIRGNQ 130 140 150 160 170 180 LEDGSFQGDR FGEVDTRFVY TALSALSILG ELTSEVVDPA VDFVLKCYNF DGGFGLCPNA 190 200 210 220 230 240 ESHAAQAFTC LGALAIANKL DMLSDDQLEE IGWWLCERQL PEGGLNGRPS KLPDVCYSWW 250 260 270 280 290 300 VLSSLAIIGR LDWINYEKLT EFILKCQDEK KGGISDRPEN EVDVFHTVFG VAGLSLMGYD 310 320 NLVPIDPIYC MPKSVTSKFK KYPYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An essential gene in Saccharomyces cerevisiae shares an upstream regulatory element with PRP4." Petersen-Bjoern S., Harrington T.R., Friesen J.D. Yeast 6:345-352(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Dependence of Ypt1 and Sec4 membrane attachment on Bet2." Rossi G., Jiang Y., Newman A.P., Ferro-Novick S. Nature 351:158-161(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle." Petersen-Bjoern S., Soltyk A., Beggs J.D., Friesen J.D. Mol. Cell. Biol. 9:3698-3709(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-187.
[7]	"Bet2p and Mad2p are components of a prenyltransferase that adds geranylgeranyl onto Ypt1p and Sec4p." Jiang Y., Rossi G., Ferro-Novick S. Nature 366:84-86(1993) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M29585 Genomic DNA. Translation: AAA66939.1. M26597 Genomic DNA. Translation: AAA79331.1. U25842 Genomic DNA. Translation: AAB68110.1. AY558067 Genomic DNA. Translation: AAS56393.1. BK006949 Genomic DNA. Translation: DAA11593.1.
PIR	S59834.
RefSeq	NP_015502.1. NM_001184273.1.
3D structure databases
ProteinModelPortal	P20133.
SMR	P20133. Positions 6-323.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2214N.
IntAct	P20133. 3 interactions.
MINT	MINT-642059.
STRING	4932.YPR176C.
Proteomic databases
PaxDb	P20133.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPR176C; YPR176C; YPR176C.
GeneID	856306.
KEGG	sce:YPR176C.
Organism-specific databases
CYGD	YPR176c.
SGD	S000006380. BET2.
Phylogenomic databases
eggNOG	COG5029.
GeneTree	ENSGT00530000063392.
HOGENOM	HOG000180334.
KO	K05956.
OMA	ERQDYES.
OrthoDB	EOG43V34B.
Gene expression databases
Genevestigator	P20133.
GermOnline	YPR176C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001330. Prenyltrans. IPR026873. Ptb1. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
PANTHER	PTHR11774:SF5. PTHR11774:SF5. 1 hit.
Pfam	PF00432. Prenyltrans. 5 hits. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 1 hit.
ProtoNet	Search...
Other
NextBio	981673.

Entry information

Entry name

PGTB2_YEAST

Accession

Primary (citable) accession number: P20133
Secondary accession number(s): D6W4H7, P32433

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 124 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program