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Protein

Geranylgeranyl transferase type-2 subunit beta

Gene

BET2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to proteins having the C-terminal -XCC or -XCXC, where both cysteines may become modified. Acts on YPT1 and SEC4.

Miscellaneous

Present with 1520 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi234ZincBy similarity1
Metal bindingi234Zinc; catalyticBy similarity1
Metal bindingi236ZincBy similarity1
Metal bindingi236Zinc; catalyticBy similarity1
Metal bindingi286ZincBy similarity1
Metal bindingi286Zinc; via tele nitrogen; catalyticBy similarity1

GO - Molecular functioni

  • Rab geranylgeranyltransferase activity Source: SGD
  • Rab GTPase binding Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: SGD
  • protein geranylgeranylation Source: SGD
  • protein targeting to membrane Source: SGD

Keywordsi

Molecular functionPrenyltransferase, Transferase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-1
ReactomeiR-SCE-6803205 TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain
R-SCE-8873719 RAB geranylgeranylation

Names & Taxonomyi

Protein namesi
Recommended name:
Geranylgeranyl transferase type-2 subunit beta (EC:2.5.1.60)
Alternative name(s):
Geranylgeranyl transferase type II subunit beta
Short name:
GGTase-II-beta
Type II protein geranyl-geranyltransferase subunit beta
Short name:
PGGT
YPT1/SEC4 proteins geranylgeranyltransferase subunit beta
Gene namesi
Name:BET2
Ordered Locus Names:YPR176C
ORF Names:P9705.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR176C
SGDiS000006380 BET2

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001197771 – 325Geranylgeranyl transferase type-2 subunit betaAdd BLAST325

Proteomic databases

MaxQBiP20133
PaxDbiP20133
PRIDEiP20133

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.

Binary interactionsi

WithEntry#Exp.IntActNotes
BET4Q006183EBI-3559,EBI-3573

GO - Molecular functioni

Protein-protein interaction databases

BioGridi36349, 356 interactors
ComplexPortaliCPX-1636 Protein geranylgeranyltransferase type II complex
DIPiDIP-2214N
IntActiP20133, 3 interactors
STRINGi4932.YPR176C

Structurei

3D structure databases

ProteinModelPortaliP20133
SMRiP20133
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati9 – 50PFTB 1Add BLAST42
Repeati57 – 99PFTB 2Add BLAST43
Repeati109 – 150PFTB 3Add BLAST42
Repeati157 – 198PFTB 4Add BLAST42
Repeati208 – 249PFTB 5Add BLAST42
Repeati256 – 298PFTB 6Add BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni183 – 185Geranylgeranyl diphosphate bindingBy similarity3
Regioni228 – 240Geranylgeranyl diphosphate bindingBy similarityAdd BLAST13

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00530000063392
HOGENOMiHOG000180334
InParanoidiP20133
KOiK05956
OMAiWGEEDTR
OrthoDBiEOG092C3IJW

Family and domain databases

CDDicd02894 GGTase-II, 1 hit
InterProiView protein in InterPro
IPR001330 PFTB_repeat
IPR026873 Ptb1
IPR008930 Terpenoid_cyclase/PrenylTrfase
PfamiView protein in Pfam
PF00432 Prenyltrans, 5 hits
SUPFAMiSSF48239 SSF48239, 1 hit

Sequencei

Sequence statusi: Complete.

P20133-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGSLTLLKE KHIRYIESLD TKKHNFEYWL TEHLRLNGIY WGLTALCVLD
60 70 80 90 100
SPETFVKEEV ISFVLSCWDD KYGAFAPFPR HDAHLLTTLS AVQILATYDA
110 120 130 140 150
LDVLGKDRKV RLISFIRGNQ LEDGSFQGDR FGEVDTRFVY TALSALSILG
160 170 180 190 200
ELTSEVVDPA VDFVLKCYNF DGGFGLCPNA ESHAAQAFTC LGALAIANKL
210 220 230 240 250
DMLSDDQLEE IGWWLCERQL PEGGLNGRPS KLPDVCYSWW VLSSLAIIGR
260 270 280 290 300
LDWINYEKLT EFILKCQDEK KGGISDRPEN EVDVFHTVFG VAGLSLMGYD
310 320
NLVPIDPIYC MPKSVTSKFK KYPYK
Length:325
Mass (Da):36,666
Last modified:February 1, 1996 - v3
Checksum:i6A9C44DFBE62AAFC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22K → N (PubMed:2204247).Curated1
Sequence conflicti22K → N (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29585 Genomic DNA Translation: AAA66939.1
M26597 Genomic DNA Translation: AAA79331.1
U25842 Genomic DNA Translation: AAB68110.1
AY558067 Genomic DNA Translation: AAS56393.1
BK006949 Genomic DNA Translation: DAA11593.1
PIRiS59834
RefSeqiNP_015502.1, NM_001184273.1

Genome annotation databases

EnsemblFungiiYPR176C; YPR176C; YPR176C
GeneIDi856306
KEGGisce:YPR176C

Similar proteinsi

Entry informationi

Entry nameiPGTB2_YEAST
AccessioniPrimary (citable) accession number: P20133
Secondary accession number(s): D6W4H7, P32433
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1996
Last modified: June 20, 2018
This is version 164 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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