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P20133

- PGTB2_YEAST

UniProt

P20133 - PGTB2_YEAST

Protein

Geranylgeranyl transferase type-2 subunit beta

Gene

BET2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 3 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to proteins having the C-terminal -XCC or -XCXC, where both cysteines may become modified. Acts on YPT1 and SEC4.

    Catalytic activityi

    Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi234 – 2341ZincBy similarity
    Metal bindingi234 – 2341Zinc; catalyticBy similarity
    Metal bindingi236 – 2361ZincBy similarity
    Metal bindingi236 – 2361Zinc; catalyticBy similarity
    Metal bindingi286 – 2861ZincBy similarity
    Metal bindingi286 – 2861Zinc; via tele nitrogen; catalyticBy similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. Rab geranylgeranyltransferase activity Source: SGD
    3. Rab GTPase binding Source: UniProtKB
    4. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. ER to Golgi vesicle-mediated transport Source: SGD
    2. protein geranylgeranylation Source: SGD
    3. protein targeting to membrane Source: SGD

    Keywords - Molecular functioni

    Prenyltransferase, Transferase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:MONOMER3O-1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Geranylgeranyl transferase type-2 subunit beta (EC:2.5.1.60)
    Alternative name(s):
    Geranylgeranyl transferase type II subunit beta
    Short name:
    GGTase-II-beta
    Type II protein geranyl-geranyltransferase subunit beta
    Short name:
    PGGT
    YPT1/SEC4 proteins geranylgeranyltransferase subunit beta
    Gene namesi
    Name:BET2
    Ordered Locus Names:YPR176C
    ORF Names:P9705.12
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR176c.
    SGDiS000006380. BET2.

    Subcellular locationi

    GO - Cellular componenti

    1. Rab-protein geranylgeranyltransferase complex Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 325325Geranylgeranyl transferase type-2 subunit betaPRO_0000119777Add
    BLAST

    Proteomic databases

    MaxQBiP20133.
    PaxDbiP20133.

    Expressioni

    Gene expression databases

    GenevestigatoriP20133.

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta subunit.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BET4Q006183EBI-3559,EBI-3573

    Protein-protein interaction databases

    BioGridi36349. 46 interactions.
    DIPiDIP-2214N.
    IntActiP20133. 3 interactions.
    MINTiMINT-642059.
    STRINGi4932.YPR176C.

    Structurei

    3D structure databases

    ProteinModelPortaliP20133.
    SMRiP20133. Positions 3-325.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati9 – 5042PFTB 1Add
    BLAST
    Repeati57 – 9943PFTB 2Add
    BLAST
    Repeati109 – 15042PFTB 3Add
    BLAST
    Repeati157 – 19842PFTB 4Add
    BLAST
    Repeati208 – 24942PFTB 5Add
    BLAST
    Repeati256 – 29843PFTB 6Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni183 – 1853Geranylgeranyl diphosphate bindingBy similarity
    Regioni228 – 24013Geranylgeranyl diphosphate bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 6 PFTB repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5029.
    GeneTreeiENSGT00530000063392.
    HOGENOMiHOG000180334.
    KOiK05956.
    OMAiERQDYES.
    OrthoDBiEOG7FJH9X.

    Family and domain databases

    Gene3Di1.50.10.20. 1 hit.
    InterProiIPR001330. Prenyltrans.
    IPR026873. Ptb1.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    [Graphical view]
    PANTHERiPTHR11774:SF9. PTHR11774:SF9. 1 hit.
    PfamiPF00432. Prenyltrans. 5 hits.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P20133-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGSLTLLKE KHIRYIESLD TKKHNFEYWL TEHLRLNGIY WGLTALCVLD    50
    SPETFVKEEV ISFVLSCWDD KYGAFAPFPR HDAHLLTTLS AVQILATYDA 100
    LDVLGKDRKV RLISFIRGNQ LEDGSFQGDR FGEVDTRFVY TALSALSILG 150
    ELTSEVVDPA VDFVLKCYNF DGGFGLCPNA ESHAAQAFTC LGALAIANKL 200
    DMLSDDQLEE IGWWLCERQL PEGGLNGRPS KLPDVCYSWW VLSSLAIIGR 250
    LDWINYEKLT EFILKCQDEK KGGISDRPEN EVDVFHTVFG VAGLSLMGYD 300
    NLVPIDPIYC MPKSVTSKFK KYPYK 325
    Length:325
    Mass (Da):36,666
    Last modified:February 1, 1996 - v3
    Checksum:i6A9C44DFBE62AAFC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti22 – 221K → N(PubMed:2204247)Curated
    Sequence conflicti22 – 221K → N(PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M29585 Genomic DNA. Translation: AAA66939.1.
    M26597 Genomic DNA. Translation: AAA79331.1.
    U25842 Genomic DNA. Translation: AAB68110.1.
    AY558067 Genomic DNA. Translation: AAS56393.1.
    BK006949 Genomic DNA. Translation: DAA11593.1.
    PIRiS59834.
    RefSeqiNP_015502.1. NM_001184273.1.

    Genome annotation databases

    EnsemblFungiiYPR176C; YPR176C; YPR176C.
    GeneIDi856306.
    KEGGisce:YPR176C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M29585 Genomic DNA. Translation: AAA66939.1 .
    M26597 Genomic DNA. Translation: AAA79331.1 .
    U25842 Genomic DNA. Translation: AAB68110.1 .
    AY558067 Genomic DNA. Translation: AAS56393.1 .
    BK006949 Genomic DNA. Translation: DAA11593.1 .
    PIRi S59834.
    RefSeqi NP_015502.1. NM_001184273.1.

    3D structure databases

    ProteinModelPortali P20133.
    SMRi P20133. Positions 3-325.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36349. 46 interactions.
    DIPi DIP-2214N.
    IntActi P20133. 3 interactions.
    MINTi MINT-642059.
    STRINGi 4932.YPR176C.

    Proteomic databases

    MaxQBi P20133.
    PaxDbi P20133.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR176C ; YPR176C ; YPR176C .
    GeneIDi 856306.
    KEGGi sce:YPR176C.

    Organism-specific databases

    CYGDi YPR176c.
    SGDi S000006380. BET2.

    Phylogenomic databases

    eggNOGi COG5029.
    GeneTreei ENSGT00530000063392.
    HOGENOMi HOG000180334.
    KOi K05956.
    OMAi ERQDYES.
    OrthoDBi EOG7FJH9X.

    Enzyme and pathway databases

    BioCyci YEAST:MONOMER3O-1.

    Miscellaneous databases

    NextBioi 981673.
    PROi P20133.

    Gene expression databases

    Genevestigatori P20133.

    Family and domain databases

    Gene3Di 1.50.10.20. 1 hit.
    InterProi IPR001330. Prenyltrans.
    IPR026873. Ptb1.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    [Graphical view ]
    PANTHERi PTHR11774:SF9. PTHR11774:SF9. 1 hit.
    Pfami PF00432. Prenyltrans. 5 hits.
    [Graphical view ]
    SUPFAMi SSF48239. SSF48239. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "An essential gene in Saccharomyces cerevisiae shares an upstream regulatory element with PRP4."
      Petersen-Bjoern S., Harrington T.R., Friesen J.D.
      Yeast 6:345-352(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Dependence of Ypt1 and Sec4 membrane attachment on Bet2."
      Rossi G., Jiang Y., Newman A.P., Ferro-Novick S.
      Nature 351:158-161(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle."
      Petersen-Bjoern S., Soltyk A., Beggs J.D., Friesen J.D.
      Mol. Cell. Biol. 9:3698-3709(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-187.
    7. "Bet2p and Mad2p are components of a prenyltransferase that adds geranylgeranyl onto Ypt1p and Sec4p."
      Jiang Y., Rossi G., Ferro-Novick S.
      Nature 366:84-86(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPGTB2_YEAST
    AccessioniPrimary (citable) accession number: P20133
    Secondary accession number(s): D6W4H7, P32433
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 136 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1520 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3