SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P20133

- PGTB2_YEAST

UniProt

P20133 - PGTB2_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Geranylgeranyl transferase type-2 subunit beta
Gene
BET2, YPR176C, P9705.12
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to proteins having the C-terminal -XCC or -XCXC, where both cysteines may become modified. Acts on YPT1 and SEC4.

Catalytic activityi

Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi234 – 2341Zinc By similarity
Metal bindingi234 – 2341Zinc; catalytic By similarity
Metal bindingi236 – 2361Zinc By similarity
Metal bindingi236 – 2361Zinc; catalytic By similarity
Metal bindingi286 – 2861Zinc By similarity
Metal bindingi286 – 2861Zinc; via tele nitrogen; catalytic By similarity

GO - Molecular functioni

  1. Rab GTPase binding Source: UniProtKB
  2. Rab geranylgeranyltransferase activity Source: SGD
  3. protein binding Source: IntAct
  4. zinc ion binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. ER to Golgi vesicle-mediated transport Source: SGD
  2. protein geranylgeranylation Source: SGD
  3. protein targeting to membrane Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Prenyltransferase, Transferase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-1.

Names & Taxonomyi

Protein namesi
Recommended name:
Geranylgeranyl transferase type-2 subunit beta (EC:2.5.1.60)
Alternative name(s):
Geranylgeranyl transferase type II subunit beta
Short name:
GGTase-II-beta
Type II protein geranyl-geranyltransferase subunit beta
Short name:
PGGT
YPT1/SEC4 proteins geranylgeranyltransferase subunit beta
Gene namesi
Name:BET2
Ordered Locus Names:YPR176C
ORF Names:P9705.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR176c.
SGDiS000006380. BET2.

Subcellular locationi

GO - Cellular componenti

  1. Rab-protein geranylgeranyltransferase complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 325325Geranylgeranyl transferase type-2 subunit beta
PRO_0000119777Add
BLAST

Proteomic databases

MaxQBiP20133.
PaxDbiP20133.

Expressioni

Gene expression databases

GenevestigatoriP20133.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.

Binary interactionsi

WithEntry#Exp.IntActNotes
BET4Q006183EBI-3559,EBI-3573

Protein-protein interaction databases

BioGridi36349. 46 interactions.
DIPiDIP-2214N.
IntActiP20133. 3 interactions.
MINTiMINT-642059.
STRINGi4932.YPR176C.

Structurei

3D structure databases

ProteinModelPortaliP20133.
SMRiP20133. Positions 3-325.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati9 – 5042PFTB 1
Add
BLAST
Repeati57 – 9943PFTB 2
Add
BLAST
Repeati109 – 15042PFTB 3
Add
BLAST
Repeati157 – 19842PFTB 4
Add
BLAST
Repeati208 – 24942PFTB 5
Add
BLAST
Repeati256 – 29843PFTB 6
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni183 – 1853Geranylgeranyl diphosphate binding By similarity
Regioni228 – 24013Geranylgeranyl diphosphate binding By similarity
Add
BLAST

Sequence similaritiesi

Contains 6 PFTB repeats.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5029.
GeneTreeiENSGT00530000063392.
HOGENOMiHOG000180334.
KOiK05956.
OMAiERQDYES.
OrthoDBiEOG7FJH9X.

Family and domain databases

Gene3Di1.50.10.20. 1 hit.
InterProiIPR001330. Prenyltrans.
IPR026873. Ptb1.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PANTHERiPTHR11774:SF9. PTHR11774:SF9. 1 hit.
PfamiPF00432. Prenyltrans. 5 hits.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.

Sequencei

Sequence statusi: Complete.

P20133-1 [UniParc]FASTAAdd to Basket

« Hide

MSGSLTLLKE KHIRYIESLD TKKHNFEYWL TEHLRLNGIY WGLTALCVLD    50
SPETFVKEEV ISFVLSCWDD KYGAFAPFPR HDAHLLTTLS AVQILATYDA 100
LDVLGKDRKV RLISFIRGNQ LEDGSFQGDR FGEVDTRFVY TALSALSILG 150
ELTSEVVDPA VDFVLKCYNF DGGFGLCPNA ESHAAQAFTC LGALAIANKL 200
DMLSDDQLEE IGWWLCERQL PEGGLNGRPS KLPDVCYSWW VLSSLAIIGR 250
LDWINYEKLT EFILKCQDEK KGGISDRPEN EVDVFHTVFG VAGLSLMGYD 300
NLVPIDPIYC MPKSVTSKFK KYPYK 325
Length:325
Mass (Da):36,666
Last modified:February 1, 1996 - v3
Checksum:i6A9C44DFBE62AAFC
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221K → N1 Publication
Sequence conflicti22 – 221K → N1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M29585 Genomic DNA. Translation: AAA66939.1.
M26597 Genomic DNA. Translation: AAA79331.1.
U25842 Genomic DNA. Translation: AAB68110.1.
AY558067 Genomic DNA. Translation: AAS56393.1.
BK006949 Genomic DNA. Translation: DAA11593.1.
PIRiS59834.
RefSeqiNP_015502.1. NM_001184273.1.

Genome annotation databases

EnsemblFungiiYPR176C; YPR176C; YPR176C.
GeneIDi856306.
KEGGisce:YPR176C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M29585 Genomic DNA. Translation: AAA66939.1 .
M26597 Genomic DNA. Translation: AAA79331.1 .
U25842 Genomic DNA. Translation: AAB68110.1 .
AY558067 Genomic DNA. Translation: AAS56393.1 .
BK006949 Genomic DNA. Translation: DAA11593.1 .
PIRi S59834.
RefSeqi NP_015502.1. NM_001184273.1.

3D structure databases

ProteinModelPortali P20133.
SMRi P20133. Positions 3-325.
ModBasei Search...

Protein-protein interaction databases

BioGridi 36349. 46 interactions.
DIPi DIP-2214N.
IntActi P20133. 3 interactions.
MINTi MINT-642059.
STRINGi 4932.YPR176C.

Proteomic databases

MaxQBi P20133.
PaxDbi P20133.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR176C ; YPR176C ; YPR176C .
GeneIDi 856306.
KEGGi sce:YPR176C.

Organism-specific databases

CYGDi YPR176c.
SGDi S000006380. BET2.

Phylogenomic databases

eggNOGi COG5029.
GeneTreei ENSGT00530000063392.
HOGENOMi HOG000180334.
KOi K05956.
OMAi ERQDYES.
OrthoDBi EOG7FJH9X.

Enzyme and pathway databases

BioCyci YEAST:MONOMER3O-1.

Miscellaneous databases

NextBioi 981673.
PROi P20133.

Gene expression databases

Genevestigatori P20133.

Family and domain databases

Gene3Di 1.50.10.20. 1 hit.
InterProi IPR001330. Prenyltrans.
IPR026873. Ptb1.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view ]
PANTHERi PTHR11774:SF9. PTHR11774:SF9. 1 hit.
Pfami PF00432. Prenyltrans. 5 hits.
[Graphical view ]
SUPFAMi SSF48239. SSF48239. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An essential gene in Saccharomyces cerevisiae shares an upstream regulatory element with PRP4."
    Petersen-Bjoern S., Harrington T.R., Friesen J.D.
    Yeast 6:345-352(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Dependence of Ypt1 and Sec4 membrane attachment on Bet2."
    Rossi G., Jiang Y., Newman A.P., Ferro-Novick S.
    Nature 351:158-161(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle."
    Petersen-Bjoern S., Soltyk A., Beggs J.D., Friesen J.D.
    Mol. Cell. Biol. 9:3698-3709(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-187.
  7. "Bet2p and Mad2p are components of a prenyltransferase that adds geranylgeranyl onto Ypt1p and Sec4p."
    Jiang Y., Rossi G., Ferro-Novick S.
    Nature 366:84-86(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPGTB2_YEAST
AccessioniPrimary (citable) accession number: P20133
Secondary accession number(s): D6W4H7, P32433
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1520 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi