Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P20133 (PGTB2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Geranylgeranyl transferase type-2 subunit beta

EC=2.5.1.60
Alternative name(s):
Geranylgeranyl transferase type II subunit beta
Short name=GGTase-II-beta
Type II protein geranyl-geranyltransferase subunit beta
Short name=PGGT
YPT1/SEC4 proteins geranylgeranyltransferase subunit beta
Gene names
Name:BET2
Ordered Locus Names:YPR176C
ORF Names:P9705.12
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to proteins having the C-terminal -XCC or -XCXC, where both cysteines may become modified. Acts on YPT1 and SEC4.

Catalytic activity

Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Heterodimer of an alpha and a beta subunit.

Miscellaneous

Present with 1520 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein prenyltransferase subunit beta family.

Contains 6 PFTB repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BET4Q006183EBI-3559,EBI-3573

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 325325Geranylgeranyl transferase type-2 subunit beta
PRO_0000119777

Regions

Repeat9 – 5042PFTB 1
Repeat57 – 9943PFTB 2
Repeat109 – 15042PFTB 3
Repeat157 – 19842PFTB 4
Repeat208 – 24942PFTB 5
Repeat256 – 29843PFTB 6
Region183 – 1853Geranylgeranyl diphosphate binding By similarity
Region228 – 24013Geranylgeranyl diphosphate binding By similarity

Sites

Metal binding2341Zinc By similarity
Metal binding2341Zinc; catalytic By similarity
Metal binding2361Zinc By similarity
Metal binding2361Zinc; catalytic By similarity
Metal binding2861Zinc By similarity
Metal binding2861Zinc; via tele nitrogen; catalytic By similarity

Experimental info

Sequence conflict221K → N Ref.1
Sequence conflict221K → N Ref.5

Sequences

Sequence LengthMass (Da)Tools
P20133 [UniParc].

Last modified February 1, 1996. Version 3.
Checksum: 6A9C44DFBE62AAFC

FASTA32536,666
        10         20         30         40         50         60 
MSGSLTLLKE KHIRYIESLD TKKHNFEYWL TEHLRLNGIY WGLTALCVLD SPETFVKEEV 

        70         80         90        100        110        120 
ISFVLSCWDD KYGAFAPFPR HDAHLLTTLS AVQILATYDA LDVLGKDRKV RLISFIRGNQ 

       130        140        150        160        170        180 
LEDGSFQGDR FGEVDTRFVY TALSALSILG ELTSEVVDPA VDFVLKCYNF DGGFGLCPNA 

       190        200        210        220        230        240 
ESHAAQAFTC LGALAIANKL DMLSDDQLEE IGWWLCERQL PEGGLNGRPS KLPDVCYSWW 

       250        260        270        280        290        300 
VLSSLAIIGR LDWINYEKLT EFILKCQDEK KGGISDRPEN EVDVFHTVFG VAGLSLMGYD 

       310        320 
NLVPIDPIYC MPKSVTSKFK KYPYK 

« Hide

References

« Hide 'large scale' references
[1]"An essential gene in Saccharomyces cerevisiae shares an upstream regulatory element with PRP4."
Petersen-Bjoern S., Harrington T.R., Friesen J.D.
Yeast 6:345-352(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Dependence of Ypt1 and Sec4 membrane attachment on Bet2."
Rossi G., Jiang Y., Newman A.P., Ferro-Novick S.
Nature 351:158-161(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle."
Petersen-Bjoern S., Soltyk A., Beggs J.D., Friesen J.D.
Mol. Cell. Biol. 9:3698-3709(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-187.
[7]"Bet2p and Mad2p are components of a prenyltransferase that adds geranylgeranyl onto Ypt1p and Sec4p."
Jiang Y., Rossi G., Ferro-Novick S.
Nature 366:84-86(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M29585 Genomic DNA. Translation: AAA66939.1.
M26597 Genomic DNA. Translation: AAA79331.1.
U25842 Genomic DNA. Translation: AAB68110.1.
AY558067 Genomic DNA. Translation: AAS56393.1.
BK006949 Genomic DNA. Translation: DAA11593.1.
PIRS59834.
RefSeqNP_015502.1. NM_001184273.1.

3D structure databases

ProteinModelPortalP20133.
SMRP20133. Positions 3-325.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36349. 46 interactions.
DIPDIP-2214N.
IntActP20133. 3 interactions.
MINTMINT-642059.
STRING4932.YPR176C.

Proteomic databases

MaxQBP20133.
PaxDbP20133.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR176C; YPR176C; YPR176C.
GeneID856306.
KEGGsce:YPR176C.

Organism-specific databases

CYGDYPR176c.
SGDS000006380. BET2.

Phylogenomic databases

eggNOGCOG5029.
GeneTreeENSGT00530000063392.
HOGENOMHOG000180334.
KOK05956.
OMAERQDYES.
OrthoDBEOG7FJH9X.

Enzyme and pathway databases

BioCycYEAST:MONOMER3O-1.

Gene expression databases

GenevestigatorP20133.

Family and domain databases

Gene3D1.50.10.20. 1 hit.
InterProIPR001330. Prenyltrans.
IPR026873. Ptb1.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PANTHERPTHR11774:SF9. PTHR11774:SF9. 1 hit.
PfamPF00432. Prenyltrans. 5 hits.
[Graphical view]
SUPFAMSSF48239. SSF48239. 1 hit.
ProtoNetSearch...

Other

NextBio981673.
PROP20133.

Entry information

Entry namePGTB2_YEAST
AccessionPrimary (citable) accession number: P20133
Secondary accession number(s): D6W4H7, P32433
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families