Reviewed, UniProtKB/Swiss-Prot P20128 (POLR_TYMVA)

Names and origin

Protein names	Recommended name:     RNA replicase polyprotein     EC=2.7.7.48
Organism	Turnip yellow mosaic virus (isolate Australia)
Taxonomic identifier	12155 [NCBI]
Taxonomic lineage	Viruses › ssRNA positive-strand viruses, no DNA stage › Tymoviridae › Tymovirus
Virus host	Brassica [TaxID: 3705] Cardamine lilacina [TaxID: 82359] Malva parviflora (Little mallow) (Cheeseweed mallow) [TaxID: 145753]

Protein attributes

Sequence length	1844 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Miscellaneous	The 206 kDa protein is potentially a polyprotein (By homology to the longer protein of the Sindbis virus).
Sequence similarities	Belongs to the tymovirus NS35 RNA replicase polyprotein family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA helicase activity Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1844	1844	RNA replicase polyprotein		PRO_0000222939
Regions
	Domain	1572 – 1678	107	RdRp catalytic
	Nucleotide binding	976 – 983	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20128-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: CB447EF05F199A18	FASTA	1,844	206,511
	10 20 30 40 50 60 MAFQLALDAL APTTHRDPSL HPILESTVDS IRSSIQTYPW SIPKELLPLL NSYGIPTSGL 70 80 90 100 110 120 GTSHHPHAAH KTIETFLLCT HWSFQATTPS SVMFMKPSKF NKLAQVNSNF RELKNYRLHP 130 140 150 160 170 180 NDSTRYPFTS PDLPVFPTIF MHDALMYYHP SQIMDLFLQK PNLERLYASL VVPPEAHLSD 190 200 210 220 230 240 QSFFPKLYTY TTTRHTLHYV PEGHEAGSYN QPSDAHSWLR INSIRLGNHH LSVTILESWG 250 260 270 280 290 300 PVHSLLIQRG TPPPDPSLQA PSTPMASDLF RSYQEPRLDV VSFRIPDAIE LPQATFLQQP 310 320 330 340 350 360 LRDRLVPRAV YNALFTYTRA VRTLRTSDPA AFVRMHSSKP DHDWVTSNAW DNLQTFALLN 370 380 390 400 410 420 VPLRPNVVYH VLQSPIASLA LYLRQHWRRL TATAVPILSF LTLLQRFLPL PIPLAEVKSI 430 440 450 460 470 480 TAFRRELYRK KAPHHPLDVF HLQQHLRNHH SAISAVRPAS PPHQRLPHAL QKAALLLLRP 490 500 510 520 530 540 ISPLLTATPF FRSEQKSMLP NAELSWTLKR FALPWQASLV LLSLSESSVL LHKLFSPPTL 550 560 570 580 590 600 QAQHDTYHRH LHPGSYSLQW ERTPLSIPRT TAFLPFTPTT STAPPDHSEA SLPPAFASTS 610 620 630 640 650 660 VPRPPPVASS LGAQPPTTTA APPTPIEPTQ RAHQNSDLTL ESSTPIEPPP PPIQSSDIPP 670 680 690 700 710 720 SAPVLFPEIN SPHRFSPKLP TTPDFEPTRT SPPPSTSHQD STDPADPLMG SHLLHHSLPA 730 740 750 760 770 780 PPTHPLQSSQ LLPAPLTNDP TAIGPVLPFE ELHPRRYPEN TATFLTRLRS LPSNHLPQPT 790 800 810 820 830 840 LNCLLSAVSD QTKVSEDHLW ESLQTILPDS QLRNEEINSL GLSTEHLTAL AHLYNFQATI 850 860 870 880 890 900 YSDRGPILFG PSDTIKRIDI THTTGPPSHF SPGKRLLGSQ PSAKGHPSDS LIRAMKSFKV 910 920 930 940 950 960 SGNYLPFSEA HNHPTSISHA KNLVSNMKNG FDGILSLLDV STGQRTGPTP KDAIIQIDHY 970 980 990 1000 1010 1020 LDTNPGKTTP VVHFAGFAGC GKTYPIQQLL KTKLFKDFRV SCPTTELRTE WKTAMELHGS 1030 1040 1050 1060 1070 1080 QSWRFNTWES SILKSSRILV IDEIYKMPRG YLDLSILADP ALELVIILGD PLQGEYHSQS 1090 1100 1110 1120 1130 1140 KDSSNHRLPS ETLRLLPYID MYCWWSYRIP QCIARLFQIH SFNAWQGIIG SVSTPQDQSP 1150 1160 1170 1180 1190 1200 VLTNSHASSL TFNSLGYRSC TISSSQGLTF CDPAIIVLDN YTKWLSSANG LVALTRSRSG 1210 1220 1230 1240 1250 1260 VQFMGPSSYV GGTNGSSAMF SDAFNNSLII MDRYFPSLFP QLKLITSPLT TRSPKLNGAT 1270 1280 1290 1300 1310 1320 PSASPTHRSP NFHLPPHIPL SYDRDFVTVN PTLPDQGPET RLDTHFLPPS RLPLHFDLPP 1330 1340 1350 1360 1370 1380 AITPPPISTS VDPPQAKASP VYPGEFFDSL AAFFLPAHDP STREVLHKDQ SSNQFPWFDR 1390 1400 1410 1420 1430 1440 PFSLSCQPSS LISAKHAPNH DPTLLPASIN KRLRFRPSEA PHQITADDVV LGLQLFHSLC 1450 1460 1470 1480 1490 1500 RAYSRQPNIT VPFNPELFAE CISLNEYAQL SSKTQSTIVA NASRSDPDWR HTTVKIFAKA 1510 1520 1530 1540 1550 1560 QHKVNDGSIF GSWKACQTLA LMHDYVILVL GPVKKYQRIF DNVDRPSHIY SHCGKTPNQL 1570 1580 1590 1600 1610 1620 RDWCQEHLTH STPKIANDYT AFDQSQHGES VVLEALKMKR LNIPSHLIQL HVHLKTNVST 1630 1640 1650 1660 1670 1680 QFGPLTCMRL TGEPGTYDDN TDYNLAVIYS QYDVGSCPIM VSGDDSLIDH PLPTRHDWPS 1690 1700 1710 1720 1730 1740 VLKRLHLRFK LELTSHPLFC GYYVGPAGCI RNPLALFCKL MIAVDDDALD DRRLSYLTEF 1750 1760 1770 1780 1790 1800 TTGHLLGESL WHLLPETHVQ YQSACFDFFC RRCPKHEKML LDDSTPTLSL LERITSSPRW 1810 1820 1830 1840 LTKNAMYLLP AKLRLAITSL SQTQSFPESI EVSHAESELL HYVQ

References

[1]	"Nucleotide sequence of the genome of an Australian isolate of turnip yellow mosaic tymovirus." Keese P., Mackenzie A., Gibbs A. Virology 172:536-546(1989) [PubMed: 2800335] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	J04373 Genomic RNA. Translation: AAA46592.1.
PIR	RRWPTM. JQ0109.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR008043. Peptidase_C21. IPR001788. RNA-dep_RNA_pol_vir-typ. IPR000606. RNA_helicase1_vir. IPR007094. RNA_pol_PSvir. IPR018308. RNA_replicase_MeTrfase_typovir. [Graphical view]
Pfam	PF10641. Methyltrans_Typ. 1 hit. PF05381. Peptidase_C21. 1 hit. PF00978. RdRP_2. 1 hit. PF01443. Viral_helicase1. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
PMAP-CutDB	P20128.

Entry information

Entry name

POLR_TYMVA

Accession

Primary (citable) accession number: P20128

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)