Reviewed, UniProtKB/Swiss-Prot P20127 (POLR_OYMV)

Names and origin

Protein names	Recommended name:     RNA replicase polyprotein     EC=2.7.7.48
Organism	Ononis yellow mosaic virus
Taxonomic identifier	12153 [NCBI]
Taxonomic lineage	Viruses › ssRNA positive-strand viruses, no DNA stage › Tymoviridae › Tymovirus
Virus host	Ononis [TaxID: 58889]

Protein attributes

Sequence length	1776 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Sequence similarities	Belongs to the tymovirus NS35 RNA replicase polyprotein family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA helicase activity Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1776	1776	RNA replicase polyprotein		PRO_0000222936
Regions
	Domain	1497 – 1603	107	RdRp catalytic
	Nucleotide binding	899 – 906	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20127-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 1C2E37B9EC3A5333	FASTA	1,776	198,129
	10 20 30 40 50 60 MAFELALNAL ASSTHKDSSL NPVLNSAVQP LQTSLQNFPW IIGKEHLPFL LAAGIPTSGF 70 80 90 100 110 120 GCNPHPHAVH KVIETFLLFN HWSFMATVQA SVMFMKPSKF KKLASVNPNF SELVNYRLTA 130 140 150 160 170 180 ADSVRYPSTS TSLPKYEIVF MHDALMYFNP SQILDLFIQC PSLQRLHCSL VVPPESSFTD 190 200 210 220 230 240 LSLHPNLYTY TISGNTLHYV PEGHHAGSYD QPLDAISWLK LNSIHSPHLN LSVSKLESWG 250 260 270 280 290 300 PVHSLLITRG LPHLPSSEKQ QVSFHIPNCL PLPEATFLHQ PLRHRLVPTE VYDALFTYTR 310 320 330 340 350 360 AVRTLRTSDP AGFVRTHSNK PQYSWVTSRA WDNLQTYALL NAPVRPVVLF DFFLSPLKKF 370 380 390 400 410 420 QLFMSQHINS LVIKALPFLG LIPPLVKLTT LGFPIPSVSH FQILFFTMIG PSGQFILEAF 430 440 450 460 470 480 PSMLHPAISY LESCGLVPRL PPPVAQQLQT MGILRKRSAF SLKTSLSTVK WPSWKITALI 490 500 510 520 530 540 SALPAALSVF LKTISPLSLQ SLHDGYNAHL HPSPFNLSWS LETFHVQSPS PFLPLSLTPP 550 560 570 580 590 600 SPSEEIALPP PIFRVPPPLP AQETPSPPAP ALVPPTQPPQ PQPWEEIIST FLSSLNASSH 610 620 630 640 650 660 KPSPSSAPLE SPSPIPESFE VLAEAPQHQR SINECGALNQ LPLPVPSPLQ NSTNPKPPFP 670 680 690 700 710 720 SSDLLSDMSC TGPVVEFETI FPAEYHMSNG SFPTRLRGHP RSSAPFPQKH CLLTAVASQL 730 740 750 760 770 780 SYTEHQLWEF LCDMLPDSLL TNSEVENFGL STDHLTCLSY RLHFECIIHT SHSTIPYGIK 790 800 810 820 830 840 KASTVIQISY IDGPPKHFKA FIKLAAAAPG SNPSKSNLVR AALRFKYNDA FLPFWDAHQH 850 860 870 880 890 900 TISVPHAKNL ISNMKNGFDG ITSQLSGPSN KSPKMKLLEL DATIDVSFPR KCDVIHIAGF 910 920 930 940 950 960 PGCGKSHPIQ KLLQTPAFRH FRLSVPTNEL RSEWKRDLNL PESEVWRLCT WETALFKSSN 970 980 990 1000 1010 1020 ILVVDEIYKL PRGYLDLILL ADPSIQLVIM LGDPLQGEYH SSHPSSSNSR LESETTRLSK 1030 1040 1050 1060 1070 1080 YIDCYCWWTY RCPKAVADLF GVKTFNSNEG FIRAVLSHPP NLPNLVNSIA TANTMQSLGH 1090 1100 1110 1120 1130 1140 HALTISSSQG MTYSDPVTVL LDRHSLLITP QTALVALTRS RSGIYFIGSM YTASGSAGTS 1150 1160 1170 1180 1190 1200 YMFSCALTGL PVDMMSAFPL FHTLPLIHEP IRSRRHRLVA GHTPSLHVPP SNKWPHRLHL 1210 1220 1230 1240 1250 1260 PPHIPTSHSK DVILAHGIVA SNAPERRLTT LHLPPTRLPL HFDLESCNPS TVSTSSTSNS 1270 1280 1290 1300 1310 1320 EVPFTHAFLG ESFEELAAHF LPAHDPDLKE VTVGDQTSQQ FPYLDQPYTL SCQPSSLLAA 1330 1340 1350 1360 1370 1380 SHKPASDPTL LIFSISKRLR FRASSSPYAF TPNDLILGHL LYTNWCKAFG RCPNSTIPFN 1390 1400 1410 1420 1430 1440 PALFAECICL NEYAQLSSKT QATIVSNASR SDPDWRYTVV RIFAKSQHKV NDGSIFGSWK 1450 1460 1470 1480 1490 1500 ACQTLALMHD FVILTLGPVK KYQRIIDHYD RPNFIYTHCG KTPSELSAWS HSFLKGDAYI 1510 1520 1530 1540 1550 1560 CNDYTSFDQS QHGEAVIFES LKMHRVGIPR HLIDLHIYLK TNVSTQFGPL TCMRLTGEPG 1570 1580 1590 1600 1610 1620 TYDDNTDYNL AVIFSQYVIS DHPIMVSGDD SVICGHPPIN PNWPAVEKLL HLRFKTEETS 1630 1640 1650 1660 1670 1680 LPLFCGYYVG PTGCCRNPFA LFAKLMISYD KGNLFETLPS YLYEFSIGHR LGDVVRLLFP 1690 1700 1710 1720 1730 1740 DHLLKYYSAC WDLFCRKCTA SQKLILSFEP IPPSFFSKLA STSRWVSKVL FSDLPTKIRD 1750 1760 1770 MLISSSKLPS YHQDPRVQYL ESELLTSFNH GRLSTN

References

[1]	"Nucleotide sequence of the ononis yellow mosaic tymovirus genome." Ding S.W., Keese P., Gibbs A. Virology 172:555-563(1989) [PubMed: 2800337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	J04375 Genomic RNA. Translation: AAA46796.1.
PIR	RRWPYM. JQ0106.
RefSeq	NP_041257.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	C21.001.
Genome annotation databases
GeneID	1493968.
Enzyme and pathway databases
BRENDA	2.7.7.48. 313966.
Family and domain databases
InterPro	IPR008043. Peptidase_C21. IPR001788. RNA-dep_RNA_pol_vir-typ. IPR000606. RNA_helicase1_vir. IPR007094. RNA_pol_PSvir. IPR018308. RNA_replicase_MeTrfase_typovir. [Graphical view]
Pfam	PF10641. Methyltrans_Typ. 1 hit. PF05381. Peptidase_C21. 1 hit. PF00978. RdRP_2. 1 hit. PF01443. Viral_helicase1. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POLR_OYMV

Accession

Primary (citable) accession number: P20127

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)