P20126 (POLR_EPMV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: RNA replicase polyprotein EC=2.7.7.48
Organism	Eggplant mosaic virus [Complete proteome]
Taxonomic identifier	12151 [NCBI]
Taxonomic lineage	Viruses › ssRNA positive-strand viruses, no DNA stage › Tymovirales › Tymoviridae › Tymovirus
Virus host	Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081] Solanum melongena (Eggplant) (Aubergine) [TaxID: 4111] Solanum seaforthianum (Brazilian nightshade) [TaxID: 45840]

Protein attributes

Sequence length	1839 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Sequence similarities	Belongs to the tymovirus NS35 RNA replicase polyprotein family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW helicase activity Inferred from electronic annotation. Source: InterPro mRNA methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1839	1839	RNA replicase polyprotein		PRO_0000222934
Regions
	Domain	1567 – 1673	107	RdRp catalytic
	Nucleotide binding	965 – 972	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20126 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: FD8DC1F5115E7861	FASTA	1,839	204,732
	10 20 30 40 50 60 MAFQSALEAL NSTTHRDAST NPILNSVVEP LRDSLSLYPW LLPKEAVPHL LSWGIPNSGL 70 80 90 100 110 120 GVTPHPHPIH KTVETFLLFN HWHALARLPS TVMFMKPSKF QKLAALNPKF QELINFRLTA 130 140 150 160 170 180 ADTTRYPSTS LTFPSNSICF MHDALMYFSP AQIVDLFTQS PALETLYCSL IVPPESHFTD 190 200 210 220 230 240 LSLFPEIYTY KISGQTLHYI PENHHSGSYN QPLQAPSWLK ISSILSPSLA LSVTKLESWG 250 260 270 280 290 300 PVHSILIQRG LPPKPSLSAR PPVLPNQPPR ATTPNSQNQL LHQTSQLFFQ LQQPQLSLVS 310 320 330 340 350 360 FRIPDCVELP QATFLRQPLR HRLVPTSVYN ALFTYTRAVR TLRTSDPAGF VRTQSNKPEH 370 380 390 400 410 420 AWVTPNAWDN LQTLSVNAPH RPQVCYHFFS SPVARLKLHF AQHWRAYLLA LTPFLTTSPL 430 440 450 460 470 480 LLPLFNFNTP FPLPRLLSLF RRSVSSPRLL HSILPSQLRG AAIPNRPLPL WVTKLHHFLD 490 500 510 520 530 540 SHSLLPTPPI RPRIELQRLP LMSLIPKPKI VLPLLSLLLS SPTIYIHFFQ AQTPQQLHDN 550 560 570 580 590 600 YHLHLHPSRF ELSWTLQSYH VTQAQSFLPL LLPAPTQAQA SNPAPRPPAF HAIPLPPQPS 610 620 630 640 650 660 TSSSPPLQEP TLSPHLIHPP LTREPSPLNG CACDSALLPS TAAMTSAEHP TPLNPPTPSP 670 680 690 700 710 720 TPDVPPPDSP GNPSLLKQVP PEANLHPIHN PDLPSSTTLP SGALTLVPAK TPSIYANPTP 730 740 750 760 770 780 PSSHPFTPLA DDPTAVGPCL PFHVLHPADY FPLSAEFLTR TRHVPPSSLS HPKLNCLLTC 790 800 810 820 830 840 FSELSGHSES DLWLSLQSIL PDSQLQNPEV STLGLSTDIL TALCFIYHSS VTLHAPSGVY 850 860 870 880 890 900 HYGIASSSTV YVIHYQPGPP PHFSLSPRLA ASAPRCNPTN SRLVRQALRF KLNGEFLPFT 910 920 930 940 950 960 QAYAHESSIT HAKNLISNMK NGFDGIMSSL TDSSKGPSPR EKLTTLDSLI DVAAPREVSL 970 980 990 1000 1010 1020 IHIAGFAGCG KTHPIQKLLQ TSPFHDFRIS CPTNELRSEW KRDMQPTAEN VWRFSTWESS 1030 1040 1050 1060 1070 1080 LLKHSEILVI DEIYKLPRGY LDLSILADPT LSLVIILGDP LQGEYHSTSP HSSNHFLPSE 1090 1100 1110 1120 1130 1140 VHRFKSYIDC YCFWSHRIPK QIASLFGVVC HNTNEGFVRA LTSHPPNSKN LTNATNTALS 1150 1160 1170 1180 1190 1200 LQQMGHHAIT ISARRVTFTE AHTILLDRHT NLLSPNNCLV ALTRSRTGVY FVGNLHLASN 1210 1220 1230 1240 1250 1260 SFGTNYMFSQ ALCQGTIDLN NVFPHIMPHL PKMYEPIRSR SNRFVSGSLN FRPTTNSRLL 1270 1280 1290 1300 1310 1320 SSLTKPTHLP PHIPTNHSLD VLVSNPVLLG ETLDPRLEVL HLPPTRLPLH LDLLPTVPSS 1330 1340 1350 1360 1370 1380 SSFSSVDHLF PTPISPAICG YTFENLAAFF LPAHDPDLKE VLINDQKSNQ FPYLDAPFEL 1390 1400 1410 1420 1430 1440 SCQPSSLLAP IHKPASDPTL LPGSIKKRLR FRASSSPYSI TPSDQLLGQH LFSSLCLAYG 1450 1460 1470 1480 1490 1500 RNPNSVLPFQ PELFSECICI NDYAQLSSKT QATIVANHQR SDPDWRLTAV RIFAKAQHKV 1510 1520 1530 1540 1550 1560 NDASIFSGWK ACQTLALMHG YIILVLGPVK KYQRIFDSKD RPPHIYYHCG KTPSQLSQWC 1570 1580 1590 1600 1610 1620 QTHLSGSSYI ANDYTAFDQS QHGEAVVLEC LKMRRLSIPD SLIQLHSHLK CSVDTQFGPL 1630 1640 1650 1660 1670 1680 TCMRLTGEPG TYDDNSDYNL AVIYSQYSLN GHPILISGDD SVLCGTPPPS PLWPTLKKML 1690 1700 1710 1720 1730 1740 HLRFKIERTS HPLFCGYYVS PHGAARNPYA LFAKLMICVD DKSLHDKKLS YLSEFSTGHL 1750 1760 1770 1780 1790 1800 AGDLVTSILP SHLLPYQSAV HDFFCRNCTP AEKILLSLDP IPESKILQLI LKVRWASQAF 1810 1820 1830 FSYLPQKARE LLVARSSLPS LYSNPKVSQL ESELLPFSQ

References

[1]	"Nucleotide sequence of the genome of eggplant mosaic tymovirus." Osorio-Keese M.E., Keese P., Gibbs A. Virology 172:547-554(1989) [PubMed: 2800336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04374 Genomic RNA. Translation: AAA43039.1.
PIR	RRWPEM. JQ0102.
RefSeq	NP_040968.1. NC_001480.1.
3D structure databases
ProteinModelPortal	P20126.
ModBase	Search...
Protein family/group databases
MEROPS	C21.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1493960.
Family and domain databases
InterPro	IPR000606. Helicase_domain_viral-like. IPR008043. Peptidase_C21. IPR007094. RNA-dir_pol_PSvirus. IPR002588. Tymovirus_MeTrfase. IPR001788. Tymovirus_RNA-dep_RNA_pol. [Graphical view]
Pfam	PF05381. Peptidase_C21. 1 hit. PF00978. RdRP_2. 1 hit. PF01443. Viral_helicase1. 1 hit. PF01660. Vmethyltransf. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POLR_EPMV

Accession

Primary (citable) accession number: P20126

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1996
Last modified:	September 21, 2011
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program