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P20115

- CISY4_ARATH

UniProt

P20115 - CISY4_ARATH

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Protein
Citrate synthase 4, mitochondrial
Gene
CSY4, At2g44350, F4I1.16
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei308 – 3081 By similarity
Active sitei354 – 3541 By similarity
Active sitei409 – 4091 By similarity

GO - Molecular functioni

  1. ATP binding Source: TAIR
  2. citrate (Si)-synthase activity Source: TAIR
  3. zinc ion binding Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. response to cadmium ion Source: TAIR
  3. tricarboxylic acid cycle Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciARA:AT2G44350-MONOMER.
ARA:GQT-2323-MONOMER.
UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase 4, mitochondrial (EC:2.3.3.16)
Gene namesi
Name:CSY4
Ordered Locus Names:At2g44350
ORF Names:F4I1.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G44350.

Subcellular locationi

Mitochondrion matrix 2 Publications

GO - Cellular componenti

  1. cell wall Source: TAIR
  2. chloroplast Source: TAIR
  3. mitochondrial matrix Source: UniProtKB-SubCell
  4. mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1616Mitochondrion Reviewed prediction
Add
BLAST
Chaini17 – 474458Citrate synthase 4, mitochondrial
PRO_0000005484Add
BLAST

Proteomic databases

PaxDbiP20115.
PRIDEiP20115.

Expressioni

Gene expression databases

GenevestigatoriP20115.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4378. 3 interactions.
IntActiP20115. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP20115.
SMRiP20115. Positions 40-465.

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0372.
HOGENOMiHOG000130831.
InParanoidiP20115.
KOiK01647.
OMAiMQKDSKF.
PhylomeDBiP20115.

Family and domain databases

Gene3Di1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P20115-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MVFFRSVSAF TRLRSRVQGQ QSSLSNSVRW IQMQSSTDLD LKSQLQELIP    50
EQQDRLKKLK SEHGKVQLGN ITVDMVIGGM RGMTGLLWET SLLDPEEGIR 100
FRGLSIPECQ KVLPTAQSGA EPLPEGLLWL LLTGKVPSKE QVEALSKDLA 150
NRAAVPDYVY NAIDALPSTA HPMTQFASGV MALQVQSEFQ KAYENGIHKS 200
KFWEPTYEDC LNLIARVPVV AAYVYRRMYK NGDSIPSDKS LDYGANFSHM 250
LGFDDEKVKE LMRLYITIHS DHEGGNVSAH TGHLVGSALS DPYLSFAAAL 300
NGLAGPLHGL ANQEVLLWIK SVVEECGEDI SKEQLKEYVW KTLNSGKVIP 350
GYGHGVLRNT DPRYVCQREF ALKHLPDDPL FQLVSKLYEV VPPVLTELGK 400
VKNPWPNVDA HSGVLLNHYG LTEARYYTVL FGVSRSLGIC SQLIWDRALG 450
LALERPKSVT MDWLEAHCKK ASSA 474
Length:474
Mass (Da):52,782
Last modified:January 16, 2004 - v3
Checksum:iD306BD540868FCA9
GO
Isoform 2 (identifier: P20115-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     18-18: Missing.

Show »
Length:473
Mass (Da):52,654
Checksum:iDF747D3DA8943FFB
GO

Sequence cautioni

The sequence CAA35570.1 differs from that shown. Reason: Frameshift at several positions.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei18 – 181Missing in isoform 2.
VSP_009185

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 251S → N in AAM62868. 1 Publication
Sequence conflicti52 – 532QQ → HK in CAA35570. 1 Publication
Sequence conflicti112 – 1121V → AL in CAA35570. 1 Publication
Sequence conflicti156 – 1561P → S in CAA35570. 1 Publication
Sequence conflicti257 – 2582KV → RL in CAA35570. 1 Publication
Sequence conflicti375 – 3751L → H in CAA35570. 1 Publication
Sequence conflicti383 – 3853LVS → CC in CAA35570. 1 Publication
Sequence conflicti448 – 4503ALG → ELL in CAA35570. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17528 mRNA. Translation: CAA35570.1. Frameshift.
AC004521 Genomic DNA. Translation: AAC16084.2.
CP002685 Genomic DNA. Translation: AEC10410.1.
CP002685 Genomic DNA. Translation: AEC10411.1.
AF387018 mRNA. Translation: AAK62463.1.
BT006613 mRNA. Translation: AAP31957.1.
AY085647 mRNA. Translation: AAM62868.1.
PIRiJA0149. YKMUM.
T02390.
RefSeqiNP_566016.1. NM_129998.3. [P20115-2]
NP_850415.1. NM_180084.2. [P20115-1]
UniGeneiAt.34501.
At.71156.

Genome annotation databases

EnsemblPlantsiAT2G44350.2; AT2G44350.2; AT2G44350. [P20115-1]
GeneIDi819042.
KEGGiath:AT2G44350.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17528 mRNA. Translation: CAA35570.1 . Frameshift.
AC004521 Genomic DNA. Translation: AAC16084.2 .
CP002685 Genomic DNA. Translation: AEC10410.1 .
CP002685 Genomic DNA. Translation: AEC10411.1 .
AF387018 mRNA. Translation: AAK62463.1 .
BT006613 mRNA. Translation: AAP31957.1 .
AY085647 mRNA. Translation: AAM62868.1 .
PIRi JA0149. YKMUM.
T02390.
RefSeqi NP_566016.1. NM_129998.3. [P20115-2 ]
NP_850415.1. NM_180084.2. [P20115-1 ]
UniGenei At.34501.
At.71156.

3D structure databases

ProteinModelPortali P20115.
SMRi P20115. Positions 40-465.
ModBasei Search...

Protein-protein interaction databases

BioGridi 4378. 3 interactions.
IntActi P20115. 2 interactions.

Proteomic databases

PaxDbi P20115.
PRIDEi P20115.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G44350.2 ; AT2G44350.2 ; AT2G44350 . [P20115-1 ]
GeneIDi 819042.
KEGGi ath:AT2G44350.

Organism-specific databases

GeneFarmi 4309.
TAIRi AT2G44350.

Phylogenomic databases

eggNOGi COG0372.
HOGENOMi HOG000130831.
InParanoidi P20115.
KOi K01647.
OMAi MQKDSKF.
PhylomeDBi P20115.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER00717 .
BioCyci ARA:AT2G44350-MONOMER.
ARA:GQT-2323-MONOMER.

Gene expression databases

Genevestigatori P20115.

Family and domain databases

Gene3Di 1.10.580.10. 1 hit.
InterProi IPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view ]
PANTHERi PTHR11739. PTHR11739. 1 hit.
Pfami PF00285. Citrate_synt. 1 hit.
[Graphical view ]
PRINTSi PR00143. CITRTSNTHASE.
SUPFAMi SSF48256. SSF48256. 1 hit.
TIGRFAMsi TIGR01793. cit_synth_euk. 1 hit.
PROSITEi PS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a cDNA encoding mitochondrial citrate synthase from Arabidopsis thaliana."
    Unger E.A., Hand J.M., Cashmore A.R., Vasconcelos A.C.
    Plant Mol. Biol. 13:411-418(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  6. "Proteomic analysis of the Arabidopsis cell wall reveals unexpected proteins with new cellular locations."
    Slabas A.R., Ndimba B., Simon W.J., Chivasa S.
    Biochem. Soc. Trans. 32:524-528(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, POSSIBLE SUBCELLULAR LOCATION.
  7. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.

Entry informationi

Entry nameiCISY4_ARATH
AccessioniPrimary (citable) accession number: P20115
Secondary accession number(s): O64869, Q8LE36, Q94EY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 16, 2004
Last modified: May 14, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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