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P20115 (CISY4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Citrate synthase 4, mitochondrial

EC=2.3.3.16
Gene names
Name:CSY4
Ordered Locus Names:At2g44350
ORF Names:F4I1.16
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.

Subunit structure

Homodimer.

Subcellular location

Mitochondrion matrix Ref.6 Ref.7.

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Sequence similarities

Belongs to the citrate synthase family.

Sequence caution

The sequence CAA35570.1 differs from that shown. Reason: Frameshift at several positions.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P20115-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P20115-2)

The sequence of this isoform differs from the canonical sequence as follows:
     18-18: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1616Mitochondrion Potential
Chain17 – 474458Citrate synthase 4, mitochondrial
PRO_0000005484

Sites

Active site3081 By similarity
Active site3541 By similarity
Active site4091 By similarity

Natural variations

Alternative sequence181Missing in isoform 2.
VSP_009185

Experimental info

Sequence conflict251S → N in AAM62868. Ref.5
Sequence conflict52 – 532QQ → HK in CAA35570. Ref.1
Sequence conflict1121V → AL in CAA35570. Ref.1
Sequence conflict1561P → S in CAA35570. Ref.1
Sequence conflict257 – 2582KV → RL in CAA35570. Ref.1
Sequence conflict3751L → H in CAA35570. Ref.1
Sequence conflict383 – 3853LVS → CC in CAA35570. Ref.1
Sequence conflict448 – 4503ALG → ELL in CAA35570. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 16, 2004. Version 3.
Checksum: D306BD540868FCA9

FASTA47452,782
        10         20         30         40         50         60 
MVFFRSVSAF TRLRSRVQGQ QSSLSNSVRW IQMQSSTDLD LKSQLQELIP EQQDRLKKLK 

        70         80         90        100        110        120 
SEHGKVQLGN ITVDMVIGGM RGMTGLLWET SLLDPEEGIR FRGLSIPECQ KVLPTAQSGA 

       130        140        150        160        170        180 
EPLPEGLLWL LLTGKVPSKE QVEALSKDLA NRAAVPDYVY NAIDALPSTA HPMTQFASGV 

       190        200        210        220        230        240 
MALQVQSEFQ KAYENGIHKS KFWEPTYEDC LNLIARVPVV AAYVYRRMYK NGDSIPSDKS 

       250        260        270        280        290        300 
LDYGANFSHM LGFDDEKVKE LMRLYITIHS DHEGGNVSAH TGHLVGSALS DPYLSFAAAL 

       310        320        330        340        350        360 
NGLAGPLHGL ANQEVLLWIK SVVEECGEDI SKEQLKEYVW KTLNSGKVIP GYGHGVLRNT 

       370        380        390        400        410        420 
DPRYVCQREF ALKHLPDDPL FQLVSKLYEV VPPVLTELGK VKNPWPNVDA HSGVLLNHYG 

       430        440        450        460        470 
LTEARYYTVL FGVSRSLGIC SQLIWDRALG LALERPKSVT MDWLEAHCKK ASSA 

« Hide

Isoform 2 [UniParc].

Checksum: DF747D3DA8943FFB
Show »

FASTA47352,654

References

« Hide 'large scale' references
[1]"Isolation of a cDNA encoding mitochondrial citrate synthase from Arabidopsis thaliana."
Unger E.A., Hand J.M., Cashmore A.R., Vasconcelos A.C.
Plant Mol. Biol. 13:411-418(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[6]"Proteomic analysis of the Arabidopsis cell wall reveals unexpected proteins with new cellular locations."
Slabas A.R., Ndimba B., Simon W.J., Chivasa S.
Biochem. Soc. Trans. 32:524-528(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, POSSIBLE SUBCELLULAR LOCATION.
[7]"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Landsberg erecta.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X17528 mRNA. Translation: CAA35570.1. Frameshift.
AC004521 Genomic DNA. Translation: AAC16084.2.
CP002685 Genomic DNA. Translation: AEC10410.1.
CP002685 Genomic DNA. Translation: AEC10411.1.
AF387018 mRNA. Translation: AAK62463.1.
BT006613 mRNA. Translation: AAP31957.1.
AY085647 mRNA. Translation: AAM62868.1.
PIRYKMUM. JA0149.
T02390.
RefSeqNP_566016.1. NM_129998.3. [P20115-2]
NP_850415.1. NM_180084.2. [P20115-1]
UniGeneAt.34501.
At.71156.

3D structure databases

ProteinModelPortalP20115.
SMRP20115. Positions 40-465.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid4378. 3 interactions.
IntActP20115. 2 interactions.

Proteomic databases

PaxDbP20115.
PRIDEP20115.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G44350.2; AT2G44350.2; AT2G44350. [P20115-1]
GeneID819042.
KEGGath:AT2G44350.

Organism-specific databases

GeneFarm4309.
TAIRAT2G44350.

Phylogenomic databases

eggNOGCOG0372.
HOGENOMHOG000130831.
InParanoidP20115.
KOK01647.
OMAMQKDSKF.
PhylomeDBP20115.

Enzyme and pathway databases

BioCycARA:AT2G44350-MONOMER.
ARA:GQT-2323-MONOMER.
UniPathwayUPA00223; UER00717.

Gene expression databases

GenevestigatorP20115.

Family and domain databases

Gene3D1.10.580.10. 1 hit.
InterProIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERPTHR11739. PTHR11739. 1 hit.
PfamPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSPR00143. CITRTSNTHASE.
SUPFAMSSF48256. SSF48256. 1 hit.
TIGRFAMsTIGR01793. cit_synth_euk. 1 hit.
PROSITEPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCISY4_ARATH
AccessionPrimary (citable) accession number: P20115
Secondary accession number(s): O64869, Q8LE36, Q94EY6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 16, 2004
Last modified: May 14, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names