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Protein

Citrate synthase 4, mitochondrial

Gene

CSY4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase 5, mitochondrial (CSY5), Citrate synthase 1, peroxisomal (CSY1), Citrate synthase 2, peroxisomal (CSY2), Citrate synthase 3, peroxisomal (CSY3), Citrate synthase 4, mitochondrial (CSY4)
  2. Aconitate hydratase 2, mitochondrial (ACO2), Aconitate hydratase 1 (ACO1), Aconitate hydratase 3, mitochondrial (ACO3)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei308PROSITE-ProRule annotation1
Active sitei354PROSITE-ProRule annotation1
Active sitei409PROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: TAIR
  • citrate (Si)-synthase activity Source: TAIR
  • zinc ion binding Source: TAIR

GO - Biological processi

  • citrate metabolic process Source: InterPro
  • response to cadmium ion Source: TAIR
  • tricarboxylic acid cycle Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciARA:AT2G44350-MONOMER.
ReactomeiR-ATH-71403. Citric acid cycle (TCA cycle).
UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase 4, mitochondrial (EC:2.3.3.16)
Gene namesi
Name:CSY4
Ordered Locus Names:At2g44350
ORF Names:F4I1.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G44350.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: TAIR
  • chloroplast Source: TAIR
  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 16MitochondrionSequence analysisAdd BLAST16
ChainiPRO_000000548417 – 474Citrate synthase 4, mitochondrialAdd BLAST458

Proteomic databases

PaxDbiP20115.
PRIDEiP20115.

Expressioni

Gene expression databases

GenevisibleiP20115. AT.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4378. 3 interactors.
IntActiP20115. 2 interactors.
STRINGi3702.AT2G44350.2.

Structurei

3D structure databases

ProteinModelPortaliP20115.
SMRiP20115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2617. Eukaryota.
COG0372. LUCA.
HOGENOMiHOG000130831.
InParanoidiP20115.
KOiK01647.
OMAiSANFASM.
OrthoDBiEOG0936083W.
PhylomeDBiP20115.

Family and domain databases

Gene3Di1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P20115-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVFFRSVSAF TRLRSRVQGQ QSSLSNSVRW IQMQSSTDLD LKSQLQELIP
60 70 80 90 100
EQQDRLKKLK SEHGKVQLGN ITVDMVIGGM RGMTGLLWET SLLDPEEGIR
110 120 130 140 150
FRGLSIPECQ KVLPTAQSGA EPLPEGLLWL LLTGKVPSKE QVEALSKDLA
160 170 180 190 200
NRAAVPDYVY NAIDALPSTA HPMTQFASGV MALQVQSEFQ KAYENGIHKS
210 220 230 240 250
KFWEPTYEDC LNLIARVPVV AAYVYRRMYK NGDSIPSDKS LDYGANFSHM
260 270 280 290 300
LGFDDEKVKE LMRLYITIHS DHEGGNVSAH TGHLVGSALS DPYLSFAAAL
310 320 330 340 350
NGLAGPLHGL ANQEVLLWIK SVVEECGEDI SKEQLKEYVW KTLNSGKVIP
360 370 380 390 400
GYGHGVLRNT DPRYVCQREF ALKHLPDDPL FQLVSKLYEV VPPVLTELGK
410 420 430 440 450
VKNPWPNVDA HSGVLLNHYG LTEARYYTVL FGVSRSLGIC SQLIWDRALG
460 470
LALERPKSVT MDWLEAHCKK ASSA
Length:474
Mass (Da):52,782
Last modified:January 16, 2004 - v3
Checksum:iD306BD540868FCA9
GO
Isoform 2 (identifier: P20115-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     18-18: Missing.

Show »
Length:473
Mass (Da):52,654
Checksum:iDF747D3DA8943FFB
GO

Sequence cautioni

The sequence CAA35570 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25S → N in AAM62868 (Ref. 5) Curated1
Sequence conflicti52 – 53QQ → HK in CAA35570 (PubMed:2491664).Curated2
Sequence conflicti112V → AL in CAA35570 (PubMed:2491664).Curated1
Sequence conflicti156P → S in CAA35570 (PubMed:2491664).Curated1
Sequence conflicti257 – 258KV → RL in CAA35570 (PubMed:2491664).Curated2
Sequence conflicti375L → H in CAA35570 (PubMed:2491664).Curated1
Sequence conflicti383 – 385LVS → CC in CAA35570 (PubMed:2491664).Curated3
Sequence conflicti448 – 450ALG → ELL in CAA35570 (PubMed:2491664).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00918518Missing in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17528 mRNA. Translation: CAA35570.1. Frameshift.
AC004521 Genomic DNA. Translation: AAC16084.2.
CP002685 Genomic DNA. Translation: AEC10410.1.
CP002685 Genomic DNA. Translation: AEC10411.1.
AF387018 mRNA. Translation: AAK62463.1.
BT006613 mRNA. Translation: AAP31957.1.
AY085647 mRNA. Translation: AAM62868.1.
PIRiJA0149. YKMUM.
T02390.
RefSeqiNP_566016.1. NM_129998.3. [P20115-2]
NP_850415.1. NM_180084.2. [P20115-1]
UniGeneiAt.34501.
At.71156.

Genome annotation databases

EnsemblPlantsiAT2G44350.2; AT2G44350.2; AT2G44350. [P20115-1]
GeneIDi819042.
GrameneiAT2G44350.2; AT2G44350.2; AT2G44350.
KEGGiath:AT2G44350.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17528 mRNA. Translation: CAA35570.1. Frameshift.
AC004521 Genomic DNA. Translation: AAC16084.2.
CP002685 Genomic DNA. Translation: AEC10410.1.
CP002685 Genomic DNA. Translation: AEC10411.1.
AF387018 mRNA. Translation: AAK62463.1.
BT006613 mRNA. Translation: AAP31957.1.
AY085647 mRNA. Translation: AAM62868.1.
PIRiJA0149. YKMUM.
T02390.
RefSeqiNP_566016.1. NM_129998.3. [P20115-2]
NP_850415.1. NM_180084.2. [P20115-1]
UniGeneiAt.34501.
At.71156.

3D structure databases

ProteinModelPortaliP20115.
SMRiP20115.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4378. 3 interactors.
IntActiP20115. 2 interactors.
STRINGi3702.AT2G44350.2.

Proteomic databases

PaxDbiP20115.
PRIDEiP20115.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G44350.2; AT2G44350.2; AT2G44350. [P20115-1]
GeneIDi819042.
GrameneiAT2G44350.2; AT2G44350.2; AT2G44350.
KEGGiath:AT2G44350.

Organism-specific databases

TAIRiAT2G44350.

Phylogenomic databases

eggNOGiKOG2617. Eukaryota.
COG0372. LUCA.
HOGENOMiHOG000130831.
InParanoidiP20115.
KOiK01647.
OMAiSANFASM.
OrthoDBiEOG0936083W.
PhylomeDBiP20115.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.
BioCyciARA:AT2G44350-MONOMER.
ReactomeiR-ATH-71403. Citric acid cycle (TCA cycle).

Miscellaneous databases

PROiP20115.

Gene expression databases

GenevisibleiP20115. AT.

Family and domain databases

Gene3Di1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCISY4_ARATH
AccessioniPrimary (citable) accession number: P20115
Secondary accession number(s): O64869, Q8LE36, Q94EY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 16, 2004
Last modified: November 2, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.