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Protein

Citrate synthase 4, mitochondrial

Gene

CSY4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase 5, mitochondrial (CSY5), Citrate synthase 1, peroxisomal (CSY1), Citrate synthase 2, peroxisomal (CSY2), Citrate synthase 3, peroxisomal (CSY3), Citrate synthase 4, mitochondrial (CSY4)
  2. Aconitate hydratase 3, mitochondrial (ACO3), Aconitate hydratase 1 (ACO1), Aconitate hydratase 2, mitochondrial (ACO2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei308PROSITE-ProRule annotation1
Active sitei354PROSITE-ProRule annotation1
Active sitei409PROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: TAIR
  • citrate (Si)-synthase activity Source: TAIR
  • zinc ion binding Source: TAIR

GO - Biological processi

  • response to cadmium ion Source: TAIR
  • tricarboxylic acid cycle Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

BioCyciARA:AT2G44350-MONOMER
ReactomeiR-ATH-71403 Citric acid cycle (TCA cycle)
UniPathwayiUPA00223; UER00717

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase 4, mitochondrial (EC:2.3.3.16)
Gene namesi
Name:CSY4
Ordered Locus Names:At2g44350
ORF Names:F4I1.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G44350
TAIRilocus:2050554 AT2G44350

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 16MitochondrionSequence analysisAdd BLAST16
ChainiPRO_000000548417 – 474Citrate synthase 4, mitochondrialAdd BLAST458

Proteomic databases

PaxDbiP20115
PRIDEiP20115

Expressioni

Gene expression databases

ExpressionAtlasiP20115 differential
GenevisibleiP20115 AT

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4378, 3 interactors
IntActiP20115, 2 interactors
STRINGi3702.AT2G44350.2

Structurei

3D structure databases

ProteinModelPortaliP20115
SMRiP20115
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2617 Eukaryota
COG0372 LUCA
HOGENOMiHOG000130831
InParanoidiP20115
KOiK01647
OMAiNCYPVLF
OrthoDBiEOG0936083W
PhylomeDBiP20115

Family and domain databases

Gene3Di1.10.230.10, 1 hit
1.10.580.10, 2 hits
InterProiView protein in InterPro
IPR016142 Citrate_synth-like_lrg_a-sub
IPR016143 Citrate_synth-like_sm_a-sub
IPR002020 Citrate_synthase
IPR019810 Citrate_synthase_AS
IPR010109 Citrate_synthase_euk
IPR036969 Citrate_synthase_sf
PANTHERiPTHR11739 PTHR11739, 1 hit
PfamiView protein in Pfam
PF00285 Citrate_synt, 1 hit
PRINTSiPR00143 CITRTSNTHASE
SUPFAMiSSF48256 SSF48256, 1 hit
TIGRFAMsiTIGR01793 cit_synth_euk, 1 hit
PROSITEiView protein in PROSITE
PS00480 CITRATE_SYNTHASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P20115-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVFFRSVSAF TRLRSRVQGQ QSSLSNSVRW IQMQSSTDLD LKSQLQELIP
60 70 80 90 100
EQQDRLKKLK SEHGKVQLGN ITVDMVIGGM RGMTGLLWET SLLDPEEGIR
110 120 130 140 150
FRGLSIPECQ KVLPTAQSGA EPLPEGLLWL LLTGKVPSKE QVEALSKDLA
160 170 180 190 200
NRAAVPDYVY NAIDALPSTA HPMTQFASGV MALQVQSEFQ KAYENGIHKS
210 220 230 240 250
KFWEPTYEDC LNLIARVPVV AAYVYRRMYK NGDSIPSDKS LDYGANFSHM
260 270 280 290 300
LGFDDEKVKE LMRLYITIHS DHEGGNVSAH TGHLVGSALS DPYLSFAAAL
310 320 330 340 350
NGLAGPLHGL ANQEVLLWIK SVVEECGEDI SKEQLKEYVW KTLNSGKVIP
360 370 380 390 400
GYGHGVLRNT DPRYVCQREF ALKHLPDDPL FQLVSKLYEV VPPVLTELGK
410 420 430 440 450
VKNPWPNVDA HSGVLLNHYG LTEARYYTVL FGVSRSLGIC SQLIWDRALG
460 470
LALERPKSVT MDWLEAHCKK ASSA
Length:474
Mass (Da):52,782
Last modified:January 16, 2004 - v3
Checksum:iD306BD540868FCA9
GO
Isoform 2 (identifier: P20115-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     18-18: Missing.

Show »
Length:473
Mass (Da):52,654
Checksum:iDF747D3DA8943FFB
GO

Sequence cautioni

The sequence CAA35570 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25S → N in AAM62868 (Ref. 5) Curated1
Sequence conflicti52 – 53QQ → HK in CAA35570 (PubMed:2491664).Curated2
Sequence conflicti112V → AL in CAA35570 (PubMed:2491664).Curated1
Sequence conflicti156P → S in CAA35570 (PubMed:2491664).Curated1
Sequence conflicti257 – 258KV → RL in CAA35570 (PubMed:2491664).Curated2
Sequence conflicti375L → H in CAA35570 (PubMed:2491664).Curated1
Sequence conflicti383 – 385LVS → CC in CAA35570 (PubMed:2491664).Curated3
Sequence conflicti448 – 450ALG → ELL in CAA35570 (PubMed:2491664).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00918518Missing in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17528 mRNA Translation: CAA35570.1 Frameshift.
AC004521 Genomic DNA Translation: AAC16084.2
CP002685 Genomic DNA Translation: AEC10410.1
CP002685 Genomic DNA Translation: AEC10411.1
CP002685 Genomic DNA Translation: ANM62353.1
CP002685 Genomic DNA Translation: ANM62354.1
AF387018 mRNA Translation: AAK62463.1
BT006613 mRNA Translation: AAP31957.1
AY085647 mRNA Translation: AAM62868.1
PIRiJA0149 YKMUM
T02390
RefSeqiNP_001324514.1, NM_001337082.1 [P20115-1]
NP_001324515.1, NM_001337083.1 [P20115-2]
NP_566016.1, NM_129998.3 [P20115-2]
NP_850415.1, NM_180084.2 [P20115-1]
UniGeneiAt.34501
At.71156

Genome annotation databases

EnsemblPlantsiAT2G44350.1; AT2G44350.1; AT2G44350 [P20115-2]
AT2G44350.2; AT2G44350.2; AT2G44350 [P20115-1]
AT2G44350.3; AT2G44350.3; AT2G44350 [P20115-1]
AT2G44350.4; AT2G44350.4; AT2G44350 [P20115-2]
GeneIDi819042
GrameneiAT2G44350.1; AT2G44350.1; AT2G44350 [P20115-2]
AT2G44350.2; AT2G44350.2; AT2G44350 [P20115-1]
AT2G44350.3; AT2G44350.3; AT2G44350 [P20115-1]
AT2G44350.4; AT2G44350.4; AT2G44350 [P20115-2]
KEGGiath:AT2G44350

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCISY4_ARATH
AccessioniPrimary (citable) accession number: P20115
Secondary accession number(s): O64869, Q8LE36, Q94EY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 16, 2004
Last modified: May 23, 2018
This is version 153 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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