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P20115

- CISY4_ARATH

UniProt

P20115 - CISY4_ARATH

Protein

Citrate synthase 4, mitochondrial

Gene

CSY4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 3 (16 Jan 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei308 – 3081PROSITE-ProRule annotation
    Active sitei354 – 3541PROSITE-ProRule annotation
    Active sitei409 – 4091PROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: TAIR
    2. citrate (Si)-synthase activity Source: TAIR
    3. zinc ion binding Source: TAIR

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. response to cadmium ion Source: TAIR
    3. tricarboxylic acid cycle Source: TAIR

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Enzyme and pathway databases

    BioCyciARA:AT2G44350-MONOMER.
    ARA:GQT-2323-MONOMER.
    UniPathwayiUPA00223; UER00717.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Citrate synthase 4, mitochondrial (EC:2.3.3.16)
    Gene namesi
    Name:CSY4
    Ordered Locus Names:At2g44350
    ORF Names:F4I1.16
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G44350.

    Subcellular locationi

    Mitochondrion matrix 1 Publication

    GO - Cellular componenti

    1. cell wall Source: TAIR
    2. chloroplast Source: TAIR
    3. mitochondrial matrix Source: UniProtKB-SubCell
    4. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1616MitochondrionSequence AnalysisAdd
    BLAST
    Chaini17 – 474458Citrate synthase 4, mitochondrialPRO_0000005484Add
    BLAST

    Proteomic databases

    PaxDbiP20115.
    PRIDEiP20115.

    Expressioni

    Gene expression databases

    GenevestigatoriP20115.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi4378. 3 interactions.
    IntActiP20115. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP20115.
    SMRiP20115. Positions 40-465.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the citrate synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0372.
    HOGENOMiHOG000130831.
    InParanoidiP20115.
    KOiK01647.
    OMAiMQKDSKF.
    PhylomeDBiP20115.

    Family and domain databases

    Gene3Di1.10.580.10. 1 hit.
    InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
    IPR002020. Citrate_synthase-like.
    IPR016141. Citrate_synthase-like_core.
    IPR019810. Citrate_synthase_AS.
    IPR010109. Citrate_synthase_euk.
    [Graphical view]
    PANTHERiPTHR11739. PTHR11739. 1 hit.
    PfamiPF00285. Citrate_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00143. CITRTSNTHASE.
    SUPFAMiSSF48256. SSF48256. 1 hit.
    TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
    PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P20115-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVFFRSVSAF TRLRSRVQGQ QSSLSNSVRW IQMQSSTDLD LKSQLQELIP    50
    EQQDRLKKLK SEHGKVQLGN ITVDMVIGGM RGMTGLLWET SLLDPEEGIR 100
    FRGLSIPECQ KVLPTAQSGA EPLPEGLLWL LLTGKVPSKE QVEALSKDLA 150
    NRAAVPDYVY NAIDALPSTA HPMTQFASGV MALQVQSEFQ KAYENGIHKS 200
    KFWEPTYEDC LNLIARVPVV AAYVYRRMYK NGDSIPSDKS LDYGANFSHM 250
    LGFDDEKVKE LMRLYITIHS DHEGGNVSAH TGHLVGSALS DPYLSFAAAL 300
    NGLAGPLHGL ANQEVLLWIK SVVEECGEDI SKEQLKEYVW KTLNSGKVIP 350
    GYGHGVLRNT DPRYVCQREF ALKHLPDDPL FQLVSKLYEV VPPVLTELGK 400
    VKNPWPNVDA HSGVLLNHYG LTEARYYTVL FGVSRSLGIC SQLIWDRALG 450
    LALERPKSVT MDWLEAHCKK ASSA 474
    Length:474
    Mass (Da):52,782
    Last modified:January 16, 2004 - v3
    Checksum:iD306BD540868FCA9
    GO
    Isoform 2 (identifier: P20115-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         18-18: Missing.

    Show »
    Length:473
    Mass (Da):52,654
    Checksum:iDF747D3DA8943FFB
    GO

    Sequence cautioni

    The sequence CAA35570.1 differs from that shown. Reason: Frameshift at several positions.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti25 – 251S → N in AAM62868. 1 PublicationCurated
    Sequence conflicti52 – 532QQ → HK in CAA35570. (PubMed:2491664)Curated
    Sequence conflicti112 – 1121V → AL in CAA35570. (PubMed:2491664)Curated
    Sequence conflicti156 – 1561P → S in CAA35570. (PubMed:2491664)Curated
    Sequence conflicti257 – 2582KV → RL in CAA35570. (PubMed:2491664)Curated
    Sequence conflicti375 – 3751L → H in CAA35570. (PubMed:2491664)Curated
    Sequence conflicti383 – 3853LVS → CC in CAA35570. (PubMed:2491664)Curated
    Sequence conflicti448 – 4503ALG → ELL in CAA35570. (PubMed:2491664)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei18 – 181Missing in isoform 2. 2 PublicationsVSP_009185

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17528 mRNA. Translation: CAA35570.1. Frameshift.
    AC004521 Genomic DNA. Translation: AAC16084.2.
    CP002685 Genomic DNA. Translation: AEC10410.1.
    CP002685 Genomic DNA. Translation: AEC10411.1.
    AF387018 mRNA. Translation: AAK62463.1.
    BT006613 mRNA. Translation: AAP31957.1.
    AY085647 mRNA. Translation: AAM62868.1.
    PIRiJA0149. YKMUM.
    T02390.
    RefSeqiNP_566016.1. NM_129998.3. [P20115-2]
    NP_850415.1. NM_180084.2. [P20115-1]
    UniGeneiAt.34501.
    At.71156.

    Genome annotation databases

    EnsemblPlantsiAT2G44350.2; AT2G44350.2; AT2G44350. [P20115-1]
    GeneIDi819042.
    KEGGiath:AT2G44350.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17528 mRNA. Translation: CAA35570.1 . Frameshift.
    AC004521 Genomic DNA. Translation: AAC16084.2 .
    CP002685 Genomic DNA. Translation: AEC10410.1 .
    CP002685 Genomic DNA. Translation: AEC10411.1 .
    AF387018 mRNA. Translation: AAK62463.1 .
    BT006613 mRNA. Translation: AAP31957.1 .
    AY085647 mRNA. Translation: AAM62868.1 .
    PIRi JA0149. YKMUM.
    T02390.
    RefSeqi NP_566016.1. NM_129998.3. [P20115-2 ]
    NP_850415.1. NM_180084.2. [P20115-1 ]
    UniGenei At.34501.
    At.71156.

    3D structure databases

    ProteinModelPortali P20115.
    SMRi P20115. Positions 40-465.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 4378. 3 interactions.
    IntActi P20115. 2 interactions.

    Proteomic databases

    PaxDbi P20115.
    PRIDEi P20115.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G44350.2 ; AT2G44350.2 ; AT2G44350 . [P20115-1 ]
    GeneIDi 819042.
    KEGGi ath:AT2G44350.

    Organism-specific databases

    GeneFarmi 4309.
    TAIRi AT2G44350.

    Phylogenomic databases

    eggNOGi COG0372.
    HOGENOMi HOG000130831.
    InParanoidi P20115.
    KOi K01647.
    OMAi MQKDSKF.
    PhylomeDBi P20115.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER00717 .
    BioCyci ARA:AT2G44350-MONOMER.
    ARA:GQT-2323-MONOMER.

    Gene expression databases

    Genevestigatori P20115.

    Family and domain databases

    Gene3Di 1.10.580.10. 1 hit.
    InterProi IPR016142. Citrate_synth-like_lrg_a-sub.
    IPR002020. Citrate_synthase-like.
    IPR016141. Citrate_synthase-like_core.
    IPR019810. Citrate_synthase_AS.
    IPR010109. Citrate_synthase_euk.
    [Graphical view ]
    PANTHERi PTHR11739. PTHR11739. 1 hit.
    Pfami PF00285. Citrate_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00143. CITRTSNTHASE.
    SUPFAMi SSF48256. SSF48256. 1 hit.
    TIGRFAMsi TIGR01793. cit_synth_euk. 1 hit.
    PROSITEi PS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a cDNA encoding mitochondrial citrate synthase from Arabidopsis thaliana."
      Unger E.A., Hand J.M., Cashmore A.R., Vasconcelos A.C.
      Plant Mol. Biol. 13:411-418(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    6. "Proteomic analysis of the Arabidopsis cell wall reveals unexpected proteins with new cellular locations."
      Slabas A.R., Ndimba B., Simon W.J., Chivasa S.
      Biochem. Soc. Trans. 32:524-528(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, POSSIBLE SUBCELLULAR LOCATION.
    7. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.

    Entry informationi

    Entry nameiCISY4_ARATH
    AccessioniPrimary (citable) accession number: P20115
    Secondary accession number(s): O64869, Q8LE36, Q94EY6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: January 16, 2004
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Citrate synthase is found in nearly all cells capable of oxidative metabolism.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3