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Protein

Alpha-actinin-2

Gene

ACTN2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi769 – 780121; possibly ancestralPROSITE-ProRule annotationAdd
BLAST
Calcium bindingi805 – 816122; possibly ancestralPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-GGA-114608. Platelet degranulation.
R-GGA-390522. Striated Muscle Contraction.
R-GGA-438066. Unblocking of NMDA receptor, glutamate binding and activation.
R-GGA-442729. CREB phosphorylation through the activation of CaMKII.
R-GGA-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-GGA-5673001. RAF/MAP kinase cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-actinin-2
Alternative name(s):
Alpha-actinin skeletal muscle isoform 2
F-actin cross-linking protein
Gene namesi
Name:ACTN2
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: Ensembl
  • filopodium Source: UniProtKB
  • focal adhesion Source: Ensembl
  • plasma membrane Source: Ensembl
  • Z disc Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 897897Alpha-actinin-2PRO_0000073437Add
BLAST

Post-translational modificationi

Ubiquitinated by FBXL22, leading to proteasomal degradation.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiP20111.
PRIDEiP20111.

Expressioni

Gene expression databases

BgeeiENSGALG00000014463.
ExpressionAtlasiP20111. baseline and differential.

Interactioni

Subunit structurei

Homodimer; antiparallel.

Protein-protein interaction databases

STRINGi9031.ENSGALP00000023309.

Structurei

3D structure databases

ProteinModelPortaliP20111.
SMRiP20111. Positions 38-264, 277-749, 826-897.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 257257Actin-bindingAdd
BLAST
Domaini41 – 145105CH 1PROSITE-ProRule annotationAdd
BLAST
Domaini154 – 257104CH 2PROSITE-ProRule annotationAdd
BLAST
Repeati284 – 394111Spectrin 1Add
BLAST
Repeati404 – 509106Spectrin 2Add
BLAST
Repeati519 – 630112Spectrin 3Add
BLAST
Repeati640 – 743104Spectrin 4Add
BLAST
Domaini756 – 79136EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini792 – 82736EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the alpha-actinin family.Curated
Contains 1 actin-binding domain.Curated
Contains 2 CH (calponin-homology) domains.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 4 spectrin repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0035. Eukaryota.
COG5069. LUCA.
HOGENOMiHOG000263418.
HOVERGENiHBG050453.
InParanoidiP20111.
KOiK05699.
OrthoDBiEOG091G020R.
PhylomeDBiP20111.
TreeFamiTF352676.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
1.10.418.10. 2 hits.
InterProiIPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR014837. EF-hand_Ca_insen.
IPR002048. EF_hand_dom.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
[Graphical view]
PfamiPF00307. CH. 2 hits.
PF08726. EFhand_Ca_insen. 1 hit.
PF00435. Spectrin. 4 hits.
[Graphical view]
SMARTiSM00033. CH. 2 hits.
SM00054. EFh. 2 hits.
SM00150. SPEC. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS00019. ACTININ_1. 1 hit.
PS00020. ACTININ_2. 1 hit.
PS50021. CH. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P20111-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSMNQIETN MQYTYNYEED EYMTQEEEWD RDLLLDPAWE KQQRKTFTAW
60 70 80 90 100
CNSHLRKAGT QIENIEEDFR NGLKLMLLLE VISGERLPKP DRGKMRFHKI
110 120 130 140 150
ANVNKALDYI ASKGVKLVSI GAEEIVDGNV KMTLGMIWTI ILRFAIQDIS
160 170 180 190 200
VEETSAKEGL LLWCQRKTAP YRNVNIQNFH LSWKDGLGLC ALIHRHRPDL
210 220 230 240 250
IDYSKLNKDD PIGNINLAME IAEKHLDIPK MLDAEDIVNT PKPDERAIMT
260 270 280 290 300
YVSCFYHAFA GAEQAETAAN RICKVLAVNQ ENERLMEEYE RLASELLEWI
310 320 330 340 350
RRTIPWLENR TPEKTMQAMQ KKLEDFRDYR RKHKPPKVQE KCQLEINFNT
360 370 380 390 400
LQTKLRISNR PAFMPSEGKM VSDIAGAWQR LEQAEKGYEE WLLNEIRRLE
410 420 430 440 450
RLEHLAEKFR QKASTHEQWA YGKEQILLQK DYESASLTEV RAMLRKHEAF
460 470 480 490 500
ESDLAAHQDR VEQIAAIAQE LNELDYHDAA SVNDRCQKIC DQWDSLGTLT
510 520 530 540 550
QKRREALERT EKLLETIDQL HLEFAKRAAP FNNWMEGAME DLQDMFIVHS
560 570 580 590 600
IEEIQSLISA HDQFKATLPE ADGERQAILS IQNEVEKVIQ SYSMRISASN
610 620 630 640 650
PYSTVTVEEI RTKWEKVKQL VPQRDQSLQE ELARQHANER LRRQFAAQAN
660 670 680 690 700
VIGPWIQTKM EEIARSSIEM TGPLEDQMNQ LKQYEQNIIN YKHNIDKLEG
710 720 730 740 750
DHQLIQEALV FDNKHTNYTM EHIRVGWELL LTTIARTINE VETQILTRDA
760 770 780 790 800
KGITQEQMND FRASFNHFDR RKNGLMDHDD FRACLISMGY DLGEAEFARI
810 820 830 840 850
MSLVDPNGQG TVTFQSFIDF MTRETADTDT AEQVIASFRI LASDKPYILA
860 870 880 890
DELRRELPPE QAQYCIKRMP QYTGPGSVPG ALDYTSFSSA LYGESDL
Length:897
Mass (Da):104,275
Last modified:February 1, 1991 - v1
Checksum:iF4FAC12F7F4C8834
GO
Isoform 2 (identifier: P20111-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     792-792: L → LDESDNLHSDEFKACLISLGEVGNDLQ

Show »
Length:923
Mass (Da):107,106
Checksum:i5EF2D3022DA85517
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei792 – 7921L → LDESDNLHSDEFKACLISLG EVGNDLQ in isoform 2. CuratedVSP_012544

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13874 mRNA. Translation: CAA32078.1.
X59247 mRNA. Translation: CAA41935.1.
X68797
, X68798, X68799, X68800, X68801, X68802 Genomic DNA. Translation: CAA48702.1.
PIRiS02032.
RefSeqiNP_990654.1. NM_205323.1. [P20111-1]
UniGeneiGga.4843.

Genome annotation databases

GeneIDi396263.
KEGGigga:396263.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13874 mRNA. Translation: CAA32078.1.
X59247 mRNA. Translation: CAA41935.1.
X68797
, X68798, X68799, X68800, X68801, X68802 Genomic DNA. Translation: CAA48702.1.
PIRiS02032.
RefSeqiNP_990654.1. NM_205323.1. [P20111-1]
UniGeneiGga.4843.

3D structure databases

ProteinModelPortaliP20111.
SMRiP20111. Positions 38-264, 277-749, 826-897.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000023309.

Proteomic databases

PaxDbiP20111.
PRIDEiP20111.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396263.
KEGGigga:396263.

Organism-specific databases

CTDi88.

Phylogenomic databases

eggNOGiKOG0035. Eukaryota.
COG5069. LUCA.
HOGENOMiHOG000263418.
HOVERGENiHBG050453.
InParanoidiP20111.
KOiK05699.
OrthoDBiEOG091G020R.
PhylomeDBiP20111.
TreeFamiTF352676.

Enzyme and pathway databases

ReactomeiR-GGA-114608. Platelet degranulation.
R-GGA-390522. Striated Muscle Contraction.
R-GGA-438066. Unblocking of NMDA receptor, glutamate binding and activation.
R-GGA-442729. CREB phosphorylation through the activation of CaMKII.
R-GGA-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-GGA-5673001. RAF/MAP kinase cascade.

Miscellaneous databases

PROiP20111.

Gene expression databases

BgeeiENSGALG00000014463.
ExpressionAtlasiP20111. baseline and differential.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
1.10.418.10. 2 hits.
InterProiIPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR014837. EF-hand_Ca_insen.
IPR002048. EF_hand_dom.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
[Graphical view]
PfamiPF00307. CH. 2 hits.
PF08726. EFhand_Ca_insen. 1 hit.
PF00435. Spectrin. 4 hits.
[Graphical view]
SMARTiSM00033. CH. 2 hits.
SM00054. EFh. 2 hits.
SM00150. SPEC. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS00019. ACTININ_1. 1 hit.
PS00020. ACTININ_2. 1 hit.
PS50021. CH. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACTN2_CHICK
AccessioniPrimary (citable) accession number: P20111
Secondary accession number(s): Q90821
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: September 7, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.