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Protein

Zinc/cadmium resistance protein

Gene

ZRC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably responsible for the uptake of zinc and cadmium ions.

GO - Molecular functioni

  • zinc ion transmembrane transporter activity Source: SGD

GO - Biological processi

  • cellular zinc ion homeostasis Source: SGD
  • regulation of sequestering of zinc ion Source: GO_Central
  • response to cadmium ion Source: UniProtKB-KW
  • response to zinc ion Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Cadmium resistance, Ion transport, Transport, Zinc transport

Keywords - Ligandi

Cadmium, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32923-MONOMER.
ReactomeiR-SCE-264876. Insulin processing.
R-SCE-435368. Zinc efflux and compartmentalization by the SLC30 family.

Protein family/group databases

TCDBi2.A.4.2.2. the cation diffusion facilitator (cdf) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc/cadmium resistance protein
Gene namesi
Name:ZRC1
Ordered Locus Names:YMR243C
ORF Names:YM9408.05C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR243C.
SGDiS000004856. ZRC1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence analysis
Transmembranei10 – 2617HelicalSequence analysisAdd
BLAST
Topological domaini27 – 4115Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei42 – 5817HelicalSequence analysisAdd
BLAST
Topological domaini59 – 7921CytoplasmicSequence analysisAdd
BLAST
Transmembranei80 – 9617HelicalSequence analysisAdd
BLAST
Topological domaini97 – 11216Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei113 – 12917HelicalSequence analysisAdd
BLAST
Topological domaini130 – 239110CytoplasmicSequence analysisAdd
BLAST
Transmembranei240 – 25617HelicalSequence analysisAdd
BLAST
Topological domaini257 – 26913Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei270 – 28617HelicalSequence analysisAdd
BLAST
Topological domaini287 – 442156CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • fungal-type vacuole Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: GO_Central
  • mitochondrial membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 442442Zinc/cadmium resistance proteinPRO_0000206103Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki357 – 357Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei387 – 3871PhosphoserineCombined sources
Modified residuei393 – 3931PhosphoserineCombined sources
Modified residuei397 – 3971PhosphoserineCombined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP20107.

PTM databases

iPTMnetiP20107.

Interactioni

Protein-protein interaction databases

BioGridi35422. 54 interactions.
DIPiDIP-2030N.
IntActiP20107. 13 interactions.
MINTiMINT-387016.

Structurei

3D structure databases

ProteinModelPortaliP20107.
SMRiP20107. Positions 14-105.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi141 – 1455His-rich (could be involved in coordination of zinc or cadmium ions)
Compositional biasi163 – 1675His-rich (could be involved in coordination of zinc or cadmium ions)
Compositional biasi216 – 2205His-rich (could be involved in coordination of zinc or cadmium ions)

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074381.
HOGENOMiHOG000166089.
InParanoidiP20107.
KOiK14688.
OMAiNPCINSH.
OrthoDBiEOG7C5MJX.

Family and domain databases

Gene3Di1.20.1510.10. 2 hits.
InterProiIPR002524. Cation_efflux.
IPR027469. Cation_efflux_TMD.
[Graphical view]
PANTHERiPTHR11562. PTHR11562. 2 hits.
PfamiPF01545. Cation_efflux. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01297. CDF. 1 hit.

Sequencei

Sequence statusi: Complete.

P20107-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITGKELRII SLLTLDTVFF LLEITIGYMS HSLALIADSF HMLNDIISLL
60 70 80 90 100
VALWAVDVAK NRGPDAKYTY GWKRAEILGA LINAVFLIAL CFSIMIEALQ
110 120 130 140 150
RLIEPQEIQN PRLVLYVGVA GLISNVVGLF LFHDHGSDSL HSHSHGSVES
160 170 180 190 200
GNNDLDIESN ATHSHSHASL PNDNLAIDED AISSPGPSGQ IGEVLPQSVV
210 220 230 240 250
NRLSNESQPL LNHDDHDHSH ESKKPGHRSL NMHGVFLHVL GDALGNIGVI
260 270 280 290 300
AAALFIWKTE YSWRYYSDPI VSLIITIIIF SSALPLSRRA SRILLQATPS
310 320 330 340 350
TISADQIQRE ILAVPGVIAV HDFHVWNLTE SIYIASIHVQ IDCAPDKFMS
360 370 380 390 400
SAKLIRKIFH QHGIHSATVQ PEFVSGDVNE DIRRRFSIIA GGSPSSSQEA
410 420 430 440
FDSHGNTEHG RKKRSPTAYG ATTASSNCIV DDAVNCNTSN CL
Length:442
Mass (Da):48,345
Last modified:February 1, 1996 - v2
Checksum:iCBE6AA9716C0CEBE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti414 – 4141R → S in CAB56542 (PubMed:2693940).Curated
Sequence conflicti417 – 4171T → I in CAB56542 (PubMed:2693940).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17537 Genomic DNA. Translation: CAB56542.1.
Z48756 Genomic DNA. Translation: CAA88653.1.
AY693213 Genomic DNA. Translation: AAT93232.1.
BK006946 Genomic DNA. Translation: DAA10144.1.
PIRiS56057.
RefSeqiNP_013970.1. NM_001182750.1.

Genome annotation databases

EnsemblFungiiYMR243C; YMR243C; YMR243C.
GeneIDi855284.
KEGGisce:YMR243C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17537 Genomic DNA. Translation: CAB56542.1.
Z48756 Genomic DNA. Translation: CAA88653.1.
AY693213 Genomic DNA. Translation: AAT93232.1.
BK006946 Genomic DNA. Translation: DAA10144.1.
PIRiS56057.
RefSeqiNP_013970.1. NM_001182750.1.

3D structure databases

ProteinModelPortaliP20107.
SMRiP20107. Positions 14-105.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35422. 54 interactions.
DIPiDIP-2030N.
IntActiP20107. 13 interactions.
MINTiMINT-387016.

Protein family/group databases

TCDBi2.A.4.2.2. the cation diffusion facilitator (cdf) family.

PTM databases

iPTMnetiP20107.

Proteomic databases

MaxQBiP20107.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR243C; YMR243C; YMR243C.
GeneIDi855284.
KEGGisce:YMR243C.

Organism-specific databases

EuPathDBiFungiDB:YMR243C.
SGDiS000004856. ZRC1.

Phylogenomic databases

GeneTreeiENSGT00550000074381.
HOGENOMiHOG000166089.
InParanoidiP20107.
KOiK14688.
OMAiNPCINSH.
OrthoDBiEOG7C5MJX.

Enzyme and pathway databases

BioCyciYEAST:G3O-32923-MONOMER.
ReactomeiR-SCE-264876. Insulin processing.
R-SCE-435368. Zinc efflux and compartmentalization by the SLC30 family.

Miscellaneous databases

PROiP20107.

Family and domain databases

Gene3Di1.20.1510.10. 2 hits.
InterProiIPR002524. Cation_efflux.
IPR027469. Cation_efflux_TMD.
[Graphical view]
PANTHERiPTHR11562. PTHR11562. 2 hits.
PfamiPF01545. Cation_efflux. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01297. CDF. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a gene conferring resistance to zinc and cadmium ions in the yeast Saccharomyces cerevisiae."
    Kamizono A., Nishizawa M., Teranishi Y., Murata K., Kimura A.
    Mol. Gen. Genet. 219:161-167(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DKD-5D-H.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-387; SER-393 AND SER-397, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-387; SER-393 AND SER-397, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-357, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiZRC1_YEAST
AccessioniPrimary (citable) accession number: P20107
Secondary accession number(s): D6W070
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1996
Last modified: July 6, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.