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Protein

Ecdysone-induced protein 74EF isoform A

Gene

Eip74EF

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi733 – 81583ETSPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • autophagy Source: FlyBase
  • cell death Source: FlyBase
  • oogenesis Source: FlyBase
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • regulation of development, heterochronic Source: FlyBase
  • regulation of transcription, DNA-templated Source: FlyBase
  • salivary gland cell autophagic cell death Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ecdysone-induced protein 74EF isoform A
Alternative name(s):
ETS-related protein E74A
Gene namesi
Name:Eip74EF
Synonyms:E74
ORF Names:CG32180
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0000567. Eip74EF.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 829829Ecdysone-induced protein 74EF isoform APRO_0000204082Add
BLAST

Expressioni

Developmental stagei

In mid instar larvae salivary glands levels are low during puff stage 1 and increase during puff stage 2 to become the predominant form in stage 3. Levels reach maximum in late larvae during puff stages 8-10, decreasing abruptly at stage 11. This expression pattern is also seen in Malpighian tubules and wing disk. Levels at puff stage 11 are appreciable in the gut and fat body. Transcripts are detected again in salivary glands from puff stages 12-14 and 17-21.1 Publication

Inductioni

The expression of this protein is developmentally regulated and is correlated with the 20-OH-ecdysone induced activity of puff 74EF.

Gene expression databases

BgeeiFBgn0000567.
ExpressionAtlasiP20105. differential.
GenevisibleiP20105. DM.

Interactioni

Protein-protein interaction databases

IntActiP20105. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP20105.
SMRiP20105. Positions 734-815.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi126 – 1294Poly-Asn
Compositional biasi187 – 1904Poly-Ser
Compositional biasi191 – 20111Poly-AlaAdd
BLAST
Compositional biasi208 – 21912Poly-AsnAdd
BLAST
Compositional biasi227 – 2326Poly-Gly
Compositional biasi318 – 3247Poly-Gln
Compositional biasi352 – 36110Poly-Gln
Compositional biasi380 – 3834Poly-Gln
Compositional biasi432 – 4354Poly-Ser
Compositional biasi456 – 47015Poly-AlaAdd
BLAST
Compositional biasi560 – 57617Poly-GlnAdd
BLAST
Compositional biasi585 – 5884Poly-Gln
Compositional biasi597 – 60711Poly-GlnAdd
BLAST
Compositional biasi641 – 6499Poly-Ala
Compositional biasi653 – 6575Poly-Ser
Compositional biasi658 – 6636Poly-Ala
Compositional biasi667 – 67913Poly-AlaAdd
BLAST

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000118996.
OrthoDBiEOG091G04CD.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P20105-1) [UniParc]FASTAAdd to basket
Also known as: E74A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPFIDDALLW CPDNDGRLVG GLDLGTCIAD DSTANGTENL NPSIQSAGNP
60 70 80 90 100
NNPQQSVGGE ILGSVESAGN ELNGAAARNV NVVVEPLCGG DSSDELFRSF
110 120 130 140 150
SESNFEIESL LSDLATVEVK VENEENNNNV ITDDDFASVA AAVVANDDLL
160 170 180 190 200
AKENAQLSAQ GLVDSVAASL ADSGDAGGQQ ALLAFGSSSS AASAIAAAAA
210 220 230 240 250
ALCGDLINNN NNNSNSNNNS NGNGNHGGGG GGASSGGGVA GDCATKLEYA
260 270 280 290 300
LMGGQPLAEE PRFVTSAAAN PLLVEKLMSK CLNIEKRMDK LSDTEIPIVK
310 320 330 340 350
QSTSPAPQQQ LQQQHHLQQQ QQQQPHNGST FAGATALLHI KTEQNTLLTP
360 370 380 390 400
LQLQQQQQQQ QGLHGAAGNG GSSNGNNAHQ QQQPLAIPQR PLLHNLLSGG
410 420 430 440 450
AIHNPHHRNY TTATTGSFPP SPADSGVSDV DSSSSGGQPC ADELKARLGM
460 470 480 490 500
PPATSASAAA AAAAAAAAAA HLHTGTFLHP NLYQNNAANS LRNIWNRSVG
510 520 530 540 550
VPDNYYGSSG AGSGGTQPGG PGNPQTPGYL TTSYFNAPTA ATAAASQRGT
560 570 580 590 600
TINGYHSLHQ QQQQQQQSQQ SQQQQQLAHQ QLSHQQQQAL HQQLSHQQQQ
610 620 630 640 650
QQQQQQQHPH SQLNGPHPHS HPHSHPHSHP HAGQHTHSTI AAAAAAAAAS
660 670 680 690 700
VVSSSSSAVA AAAMLSASAA AAATAAAAAG GSQSVIQPAT SSVSYDLSYM
710 720 730 740 750
LELGGFQQRK AKKPRKPKLE MGVKRRSREG STTYLWEFLL KLLQDREYCP
760 770 780 790 800
RFIKWTNREK GVFKLVDSKA VSRLWGMHKN KPDMNYETMG RALRYYYQRG
810 820
ILAKVDGQRL VYQFVDVPKD IIEIDCNGV
Length:829
Mass (Da):87,139
Last modified:March 15, 2004 - v2
Checksum:i7C8B07CF0A1D7F5D
GO
Isoform B (identifier: P11536-1) [UniParc]FASTAAdd to basket
Also known as: D, E74B
The sequence of this isoform can be found in the external entry P11536.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:883
Mass (Da):94,811
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37082 mRNA. Translation: AAA28493.1.
AE014296 Genomic DNA. Translation: AAF49323.2.
PIRiA34692.
RefSeqiNP_730287.1. NM_168740.3. [P20105-1]
UniGeneiDm.3983.

Genome annotation databases

EnsemblMetazoaiFBtr0075202; FBpp0074965; FBgn0000567. [P20105-1]
GeneIDi39962.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37082 mRNA. Translation: AAA28493.1.
AE014296 Genomic DNA. Translation: AAF49323.2.
PIRiA34692.
RefSeqiNP_730287.1. NM_168740.3. [P20105-1]
UniGeneiDm.3983.

3D structure databases

ProteinModelPortaliP20105.
SMRiP20105. Positions 734-815.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP20105. 2 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0075202; FBpp0074965; FBgn0000567. [P20105-1]
GeneIDi39962.

Organism-specific databases

CTDi39962.
FlyBaseiFBgn0000567. Eip74EF.

Phylogenomic databases

GeneTreeiENSGT00760000118996.
OrthoDBiEOG091G04CD.

Miscellaneous databases

ChiTaRSiEip74EF. fly.
GenomeRNAii39962.

Gene expression databases

BgeeiFBgn0000567.
ExpressionAtlasiP20105. differential.
GenevisibleiP20105. DM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiE74EA_DROME
AccessioniPrimary (citable) accession number: P20105
Secondary accession number(s): Q9VVI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: March 15, 2004
Last modified: September 7, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.