P20099 (BISC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin sulfoxide reductase
EC=1.-.-.-

Alternative name(s):
BDS reductase
BSO reductase

Gene names

Name:	bisC
Ordered Locus Names:	b3551, JW5940

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	777 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme may serve as a scavenger, allowing the cell to utilize biotin sulfoxide as a biotin source. It reduces a spontaneous oxidation product of biotin, D-biotin D-sulfoxide (BSO or BDS), back to biotin.
Cofactor	Molybdenum (molybdopterin).
Miscellaneous	Requires a small thioredoxin-like protein for activity.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.
Sequence caution	The sequence AAB18528.1 differs from that shown. Reason: Erroneous initiation. The sequence BAE77744.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular respiration Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	L-methionine-(S)-S-oxide reductase activity Inferred from direct assay PubMed 15601707. Source: EcoCyc electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from mutant phenotype PubMed 15601707. Source: EcoCyc oxidoreductase activity, acting on other nitrogenous compounds as donors Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 777	777	Biotin sulfoxide reductase		PRO_0000063219
Experimental info
	Sequence conflict	583 – 591	9	DFCRDPLAH → AFLPRSAGD in AAA23522. Ref.1
	Sequence conflict	596 – 598	3	ASG → QR in AAA23522. Ref.1
	Sequence conflict	747 – 777	31	NGCAG…PPASS → MAVRVIRRWHGWKNTTVRN in AAA23522. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P20099 [UniParc]. Last modified May 30, 2000. Version 3. Checksum: 51087D957E4FB38B	FASTA	777	85,851
	10 20 30 40 50 60 MANSSSRYSV LTAAHWGPML VETDGETVFS SRGALATGME NSLQSAVRDQ VHSNTRVRFP 70 80 90 100 110 120 MVRKGFLASP ENPQGIRGQD EFVRVSWDEA LDLIHQQHKR IREAYGPASI FAGSYGWRSN 130 140 150 160 170 180 GVLHKASTLL QRYMALAGGY TGHLGDYSTG AAQAIMPYVV GGSEVYQQQT SWPLVLEHSD 190 200 210 220 230 240 VVVLWSANPL NTLKIAWNAS DEQGLSYFSA LRDSGKKLIC IDPMRSETVD FFGDKMEWVA 250 260 270 280 290 300 PHMGTDVALM LGIAHTLVEN GWHDEAFLAR CTTGYAVFAS YLLGESDGIA KTAEWAAEIC 310 320 330 340 350 360 GVGAAKIREL AAIFHQNTTM LMAGWGMQRQ QFGEQKHWMI VTLAAMLGQI GTPGGGFGLS 370 380 390 400 410 420 YHFANGGNPT RRSAVLSSMQ GSLPGGCDAV DKIPVARIVE ALENPGGAYQ HNGMNRHFPD 430 440 450 460 470 480 IRFIWWAGGA NFTHHQDTNR LIRAWQKPEL VVISECFWTA AAKHADIVLP ATTSFERNDL 490 500 510 520 530 540 TMTGDYSNQH LVPMKQVVPP RYEARNDFDV FAELSERWEK GGYARFTEGK SELQWLETFY 550 560 570 580 590 600 NVARQRGASQ QVELPPFAEF WQANQLIEMP ENPDSERFIR FADFCRDPLA HPLKTASGKI 610 620 630 640 650 660 EIFSQRIADY GYPDCPGHPM WLEPDEWQGN AEPEQLQVLS AHPAHRLHSQ LNYSSLRELY 670 680 690 700 710 720 AVANREPVTI HPDDAQERGI QDGDTVRLWN ARGQILAGAV ISEGIKPGVI CIHEGAWPDL 730 740 750 760 770 DLTADGICKN GAVNVLTKDL PSSRLGNGCA GNTALAWLEK YNGPELTLTA FEPPASS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of bisC, the structural gene for biotin sulfoxide reductase in Escherichia coli." Pierson D.E., Campbell A. J. Bacteriol. 172:2194-2198(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M34827 Genomic DNA. Translation: AAA23522.1. Frameshift. U00039 Genomic DNA. Translation: AAB18528.1. Different initiation. U00096 Genomic DNA. Translation: AAC76575.3. AP009048 Genomic DNA. Translation: BAE77744.1. Different initiation.
PIR	S47772.
RefSeq	NP_418007.3. NC_000913.2. YP_491885.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P20099.
SMR	P20099. Positions 10-777.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b3551.
Protein family/group databases
TCDB	5.A.3.4.3. prokaryotic molybdopterin-containing oxidoreductase (PMO) family.
Proteomic databases
PaxDb	P20099.
PRIDE	P20099.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76575; AAC76575; b3551. BAE77744; BAE77744; BAE77744.
GeneID	12932253. 946915.
KEGG	ecj:Y75_p3626. eco:b3551.
PATRIC	32122574. VBIEscCol129921_3664.
Organism-specific databases
EchoBASE	EB0122.
EcoGene	EG10124. bisC.
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HOG000284391.
KO	K08351.
OMA	AWASEIC.
ProtClustDB	CLSK880720.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10124-MONOMER. ECOL316407:JW5940-MONOMER. MetaCyc:EG10124-MONOMER.
Gene expression databases
Genevestigator	P20099.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR006658. BisC. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006655. Mopterin_OxRdtase_prok_CS. [Graphical view]
Pfam	PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR00509. bisC_fam. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. False negative. PS00490. MOLYBDOPTERIN_PROK_2. 1 hit. PS00932. MOLYBDOPTERIN_PROK_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BISC_ECOLI

Accession

Primary (citable) accession number: P20099
Secondary accession number(s): Q2M7L2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	May 30, 2000
Last modified:	May 1, 2013
This is version 109 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program