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Protein

Saposin-C

Gene

PSAP

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Saposin-C
Alternative name(s):
Co-beta-glucosidase
Glucosylceramidase activator
Sphingolipid activator protein 2
Short name:
SAP-2
Gene namesi
Name:PSAP
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
Proteomesi
  • UP000005447 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8181Saposin-CPRO_0000175239Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi5 ↔ 78PROSITE-ProRule annotation
Disulfide bondi8 ↔ 72PROSITE-ProRule annotation
Glycosylationi22 – 221N-linked (GlcNAc...)
Disulfide bondi36 ↔ 47PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000001595.

Structurei

3D structure databases

ProteinModelPortaliP20097.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 8181Saposin B-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 saposin B-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1340. Eukaryota.
ENOG410XSI5. LUCA.
GeneTreeiENSGT00530000063434.
HOGENOMiHOG000049216.
InParanoidiP20097.

Family and domain databases

Gene3Di1.10.225.10. 1 hit.
InterProiIPR007856. SapB_1.
IPR008138. SapB_2.
IPR008373. Saposin.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
[Graphical view]
PfamiPF05184. SapB_1. 1 hit.
PF03489. SapB_2. 1 hit.
[Graphical view]
PRINTSiPR01797. SAPOSIN.
SMARTiSM00741. SapB. 1 hit.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
PROSITEiPS50015. SAP_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
ESVTCKACEY VVKKVMELID NNRTEEKIIH ALDSVCALLP ESVSEVCQEV
60 70 80
VDTYGDSIVA LLLQEMSPEL VCSELGLCMS G
Length:81
Mass (Da):8,852
Last modified:February 1, 1991 - v1
Checksum:iE564CE1F0A292596
GO

Sequence databases

PIRiA32026.

Genome annotation databases

EnsembliENSCPOT00000026133; ENSCPOP00000021187; ENSCPOG00000001764.

Cross-referencesi

Sequence databases

PIRiA32026.

3D structure databases

ProteinModelPortaliP20097.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000001595.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCPOT00000026133; ENSCPOP00000021187; ENSCPOG00000001764.

Phylogenomic databases

eggNOGiKOG1340. Eukaryota.
ENOG410XSI5. LUCA.
GeneTreeiENSGT00530000063434.
HOGENOMiHOG000049216.
InParanoidiP20097.

Family and domain databases

Gene3Di1.10.225.10. 1 hit.
InterProiIPR007856. SapB_1.
IPR008138. SapB_2.
IPR008373. Saposin.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
[Graphical view]
PfamiPF05184. SapB_1. 1 hit.
PF03489. SapB_2. 1 hit.
[Graphical view]
PRINTSiPR01797. SAPOSIN.
SMARTiSM00741. SapB. 1 hit.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
PROSITEiPS50015. SAP_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The activator protein for glucosylceramide beta-glucosidase from guinea pig liver. Improved isolation method and complete amino acid sequence."
    Sano A., Radin N.S., Johnson L.L., Tarr G.E.
    J. Biol. Chem. 263:19597-19601(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Tissue: Liver.

Entry informationi

Entry nameiSAP_CAVPO
AccessioniPrimary (citable) accession number: P20097
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 11, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.