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Protein

NADH-cytochrome b5 reductase 3

Gene

Cyb5r3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

Catalytic activityi

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.

Cofactori

FAD1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi132 – 147FADAdd BLAST16
Nucleotide bindingi171 – 206FADAdd BLAST36

GO - Molecular functioni

  • ADP binding Source: RGD
  • AMP binding Source: RGD
  • cytochrome-b5 reductase activity, acting on NAD(P)H Source: RGD
  • FAD binding Source: RGD
  • flavin adenine dinucleotide binding Source: RGD
  • NAD binding Source: RGD

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

BRENDAi1.6.2.2 5301
ReactomeiR-RNO-196836 Vitamin C (ascorbate) metabolism
R-RNO-211945 Phase I - Functionalization of compounds
R-RNO-6798695 Neutrophil degranulation
SABIO-RKiP20070

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-cytochrome b5 reductase 3 (EC:1.6.2.2)
Short name:
B5R
Short name:
Cytochrome b5 reductase
Alternative name(s):
Diaphorase-1
Cleaved into the following 2 chains:
Gene namesi
Name:Cyb5r3
Synonyms:Dia1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi2502 Cyb5r3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000194012 – 301NADH-cytochrome b5 reductase 3 membrane-bound formAdd BLAST300
ChainiPRO_000001940327 – 301NADH-cytochrome b5 reductase 3 soluble formAdd BLAST275

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Modified residuei42N6-acetyllysineBy similarity1
Modified residuei43PhosphotyrosineBy similarity1
Modified residuei50N6-acetyllysineBy similarity1
Modified residuei120N6-acetyllysineBy similarity1

Post-translational modificationi

Only the isoform 1 is myristoyled.

Keywords - PTMi

Acetylation, Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiP20070
PRIDEiP20070

PTM databases

iPTMnetiP20070
PhosphoSitePlusiP20070
SwissPalmiP20070

Expressioni

Tissue specificityi

Isoform 1 and isoform 3 are ubiquitously expressed. Isoform 2 is expressed only in erythroid tissues, reticulocytes and liver.1 Publication

Gene expression databases

BgeeiENSRNOG00000009592
GenevisibleiP20070 RN

Interactioni

Subunit structurei

Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MOSC2.By similarity

Protein-protein interaction databases

CORUMiP20070
IntActiP20070, 2 interactors
STRINGi10116.ENSRNOP00000012878

Structurei

Secondary structure

1301
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi43 – 52Combined sources10
Beta strandi54 – 63Combined sources10
Beta strandi78 – 85Combined sources8
Beta strandi88 – 94Combined sources7
Beta strandi104 – 111Combined sources8
Beta strandi115 – 118Combined sources4
Helixi126 – 132Combined sources7
Beta strandi139 – 146Combined sources8
Beta strandi148 – 153Combined sources6
Beta strandi156 – 159Combined sources4
Beta strandi168 – 171Combined sources4
Beta strandi173 – 180Combined sources8
Helixi181 – 183Combined sources3
Helixi184 – 196Combined sources13
Beta strandi203 – 212Combined sources10
Helixi213 – 215Combined sources3
Helixi219 – 228Combined sources10
Turni230 – 232Combined sources3
Beta strandi233 – 241Combined sources9
Beta strandi247 – 252Combined sources6
Helixi255 – 261Combined sources7
Beta strandi269 – 275Combined sources7
Helixi277 – 282Combined sources6
Helixi285 – 291Combined sources7
Helixi295 – 297Combined sources3
Beta strandi298 – 300Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I7PX-ray2.00A34-301[»]
1IB0X-ray2.30A34-301[»]
1QX4X-ray1.80A/B34-301[»]
ProteinModelPortaliP20070
SMRiP20070
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20070

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 152FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0534 Eukaryota
COG0543 LUCA
GeneTreeiENSGT00390000008881
HOGENOMiHOG000175005
HOVERGENiHBG052580
InParanoidiP20070
KOiK00326
OMAiNGPMSTH
OrthoDBiEOG091G0EIS
PhylomeDBiP20070
TreeFamiTF314333

Family and domain databases

InterProiView protein in InterPro
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR001834 NADH-Cyt_B5_reductase
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00175 NAD_binding_1, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00371 FPNCR
SUPFAMiSSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative promoter usage and alternative initiation. AlignAdd to basket

Isoform 1 (identifier: P20070-1) [UniParc]FASTAAdd to basket
Also known as: L-form reticulocyte reductase

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAQLSTLSR VVLSPVWFVY SLFMKLFQRS SPAITLENPD IKYPLRLIDK
60 70 80 90 100
EIISHDTRRF RFALPSPQHI LGLPIGQHIY LSTRIDGNLV IRPYTPVSSD
110 120 130 140 150
DDKGFVDLVV KVYFKDTHPK FPAGGKMSQY LENMNIGDTI EFRGPNGLLV
160 170 180 190 200
YQGKGKFAIR ADKKSNPVVR TVKSVGMIAG GTGITPMLQV IRAVLKDPND
210 220 230 240 250
HTVCYLLFAN QSEKDILLRP ELEELRNEHS SRFKLWYTVD KAPDAWDYSQ
260 270 280 290 300
GFVNEEMIRD HLPPPGEETL ILMCGPPPMI QFACLPNLER VGHPKERCFT

F
Note: Produced by alternative promoter usage.
Length:301
Mass (Da):34,175
Last modified:January 23, 2007 - v2
Checksum:i45431A644413905F
GO
Isoform 2 (identifier: P20070-2) [UniParc]FASTAAdd to basket
Also known as: R-form reticulocyte reductase

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: MGAQLST → MLGPLLWTASLPV

Note: Produced by alternative promoter usage.
Show »
Length:307
Mass (Da):34,866
Checksum:iBEF7A5F4F12AC7C2
GO
Isoform 3 (identifier: P20070-3) [UniParc]FASTAAdd to basket
Also known as: Soluble form

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.

Note: Produced by alternative initiation.
Show »
Length:278
Mass (Da):31,592
Checksum:i4FA80DCDADD24A1B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti105F → L in AAA41008 (PubMed:3174630).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0096601 – 23Missing in isoform 3. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_0096611 – 7MGAQLST → MLGPLLWTASLPV in isoform 2. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00636 mRNA Translation: BAA00530.1
J03867 mRNA Translation: AAA41008.1
BC062066 mRNA Translation: AAH62066.1
X65191 mRNA Translation: CAA46308.1
X65191 mRNA Translation: CAA46309.1
X65190 Genomic DNA Translation: CAA46307.1
X77117 Genomic DNA No translation available.
PIRiA40495 RDRTB5
S23641
RefSeqiNP_620232.1, NM_138877.1 [P20070-1]
XP_006242127.1, XM_006242065.3 [P20070-2]
UniGeneiRn.35994

Genome annotation databases

EnsembliENSRNOT00000064700; ENSRNOP00000061381; ENSRNOG00000009592 [P20070-1]
GeneIDi25035
KEGGirno:25035

Keywords - Coding sequence diversityi

Alternative initiation, Alternative promoter usage

Similar proteinsi

Entry informationi

Entry nameiNB5R3_RAT
AccessioniPrimary (citable) accession number: P20070
Secondary accession number(s): Q64569, Q64720
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health