Reviewed, UniProtKB/Swiss-Prot P20069 (MPPA_RAT)

Names and origin

Protein names

Recommended name:
    Mitochondrial-processing peptidase subunit alpha
    EC=3.4.24.64
Alternative name(s):
    Alpha-MPP
    P-55

Gene names

Name:	Pmpca
Synonyms:	Mppa

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The mitochondrial processing protease (MPP-I) cleaves presequences from mitochondrial protein precursors. Most MPP-I cleavage sites follow an arginine at position -2.
Catalytic activity	Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.
Subunit structure	Heterodimer of alpha and beta subunits.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the peptidase M16 family.
Caution	Does not seem to have a protease activity as it lack the zinc-binding site.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Ref.1 Inferred from direct assay. Source: RGD
Cellular component	mitochondrial matrix Ref.1 Traceable author statement. Source: RGD
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 32	32	Mitochondrion Ref.1
	Chain	33 – 524	492	Mitochondrial-processing peptidase subunit alpha		PRO_0000026769

Sequences

	Sequence		Length	Mass (Da)
	P20069-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 8BF08FBC9FF09DB2	FASTA	524	58,608
	10 20 30 40 50 60 MATAVWAAAR LLRGSAALCA RPKFGSPAHR RFSSGATYPN IPLSSPLPGV PKPIFATVDG 70 80 90 100 110 120 QEKFETKVTT LDNGLRVASQ NKFGQFCTLG ILINSGSRYE AKYLSGIAHF LEKLAFSSTA 130 140 150 160 170 180 RFDSKDEILL TLEKHGGICD CQTSRDTTMY AVSADSKGLD TVVGLLADVV LHPRLTDEEI 190 200 210 220 230 240 EMTRMAVQFE LEDLNMRPDP EPLLTEMIHE AAFRENTVGL HRFCPVENIG KIDREVLHSY 250 260 270 280 290 300 LKNYYTPDRM VLAGVGVEHE HLVECARKYL LGVQPAWGAP GAVWMLTAQW HSTRGGSSRW 310 320 330 340 350 360 RETCQMSALR PPRFQSSHIY GGARELLLLE EDFIPFAVLN MMMGGGGSFS AGGPGKGMFS 370 380 390 400 410 420 RLYLNVLNRH HWMYNATSYH HSYEDTGLLC IHASADPRQV REMVEIITKE FILMGRTVDL 430 440 450 460 470 480 VELERAKTQL MSMLMMNLES RPVIFEDVGR QVLATHSRKL PHELCTLIRN VKPEDIKRVA 490 500 510 520 SKMLRGKPAV AALGDLTDLP TYEHIQAALS SRDGRLPRTY RLFR

References

[1]

"The general mitochondrial matrix processing protease from rat liver: structural characterization of the catalytic subunit." Kleiber J., Kalousek F., Swaroop M., Rosenberg L.E. Proc. Natl. Acad. Sci. U.S.A. 87:7978-7982(1990) [PubMed: 2236012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 33-44; 113-121; 188-202; 223-231; 235-242; 362-369 AND 486-511. Strain: Sprague-Dawley. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M57728 mRNA. Translation: AAA41632.1.
IPI	IPI00195551.
PIR	A36205.
UniGene	Rn.11175
3D structure databases
HSSP	HSSP built from PDB template 1HR6 based on UniProtKB P11914.
ModBase	Search...
Protein family/group databases
MEROPS	M16.971.
Genome annotation databases
Ensembl	ENSRNOG00000026775. Rattus norvegicus. [Contig view]
Organism-specific databases
RGD	727897. Pmpca.
Phylogenomic databases
HOVERGEN	P20069.
Enzyme and pathway databases
BRENDA	3.4.24.64. 248.
Gene expression databases
ArrayExpress	P20069.
GermOnline	ENSRNOG00000026775. Rattus norvegicus.
Family and domain databases
InterPro	IPR011237. Pept_M16_core. IPR011765. Pept_M16_N. IPR001431. Pept_M16_Zn_BS. IPR007863. Peptidase_M16_C. [Graphical view]
Gene3D	G3DSA:3.30.830.10. Pept_M16_core. 2 hits.
Pfam	PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 1 hit. [Graphical view]
PROSITE	PS00143. INSULINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPPA_RAT

Accession

Primary (citable) accession number: P20069

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)