Tmsb4x

Functionⁱ

Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.

Seraspenide inhibits the entry of hematopoietic pluripotent stem cells into the S-phase.By similarity

GO - Molecular functionⁱ

actin monomer binding Source: InterPro

Complete GO annotation...

GO - Biological processⁱ

actin filament organization Source: InterPro
positive regulation of smooth muscle cell differentiation
Source: DFLAT
Traceable author statementⁱ
- 20299672
positive regulation of smooth muscle cell migration
Source: DFLAT
Traceable author statementⁱ
- 20299672
positive regulation of transcription, DNA-templated
Source: DFLAT
Traceable author statementⁱ
- 20299672
regulation of cell migration
Source: MGI
Inferred from direct assayⁱ
- 15565145

Complete GO annotation...

Keywordsⁱ

Molecular function	Actin-binding

Molecular function

Actin-binding

Enzyme and pathway databases

Reactomeⁱ	R-MMU-114608. Platelet degranulation.

Reactomeⁱ

R-MMU-114608. Platelet degranulation.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Thymosin beta-4 Short name: T beta 4 Cleaved into the following chain: Hematopoietic system regulatory peptide Alternative name(s): Seraspenide
Gene namesⁱ	Name:Tmsb4x Synonyms:Ptmb4, Tmsb4
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome X

Organism-specific databases

MGIⁱ	MGI:99510. Tmsb4x.

Subcellular locationⁱ

Cytoplasm › cytoskeleton

GO - Cellular componentⁱ

cytoskeleton Source: UniProtKB-SubCell
cytosol
Source: MGI
Inferred from direct assayⁱ
- 15565145
extracellular space
Source: BHF-UCL
Inferred from direct assayⁱ
- 24006456
nucleus
Source: MGI
Inferred from direct assayⁱ
- 15565145

Complete GO annotation...

Keywords - Cellular componentⁱ

Cytoplasm, Cytoskeleton

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000045923	1 – 50	Thymosin beta-4Add BLAST		50
PeptideⁱPRO_0000034297	8 – 11	Hematopoietic system regulatory peptideBy similarity		4

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	8	N-acetylserineBy similarity	1
Modified residueⁱ	8	PhosphoserineBy similarity	1
Modified residueⁱ	10	N6-acetyllysineBy similarity	1
Modified residueⁱ	18	N6-acetyllysineBy similarity	1
Modified residueⁱ	29	PhosphothreonineCombined sources	1
Modified residueⁱ	32	N6-acetyllysineBy similarity	1
Modified residueⁱ	37	PhosphoserineBy similarity	1
Modified residueⁱ	38	N6-acetyllysineBy similarity	1
Modified residueⁱ	40	PhosphothreonineBy similarity	1
Modified residueⁱ	45	N6-acetyllysineBy similarity	1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P20065.
PaxDbⁱ	P20065.
PeptideAtlas More...PeptideAtlasⁱ	P20065.
PRIDEⁱ	P20065.

PTM databases

iPTMnetⁱ	P20065.
PhosphoSitePlusⁱ	P20065.

Expressionⁱ

Tissue specificityⁱ

Originally found in thymus but it is widely distributed in many tissues.

Gene expression databases

Bgeeⁱ	ENSMUSG00000049775.
CleanExⁱ	MM_TMSB4X.
ExpressionAtlasⁱ	P20065. baseline and differential.
Genevisibleⁱ	P20065. MM.

Interactionⁱ

Subunit structureⁱ

Interacts with SERPINB1. Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X (By similarity).By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
STAB2	Q8WWQ8	3	EBI-7946048,EBI-7945957	From Homo sapiens.

With

Entry

#Exp.

IntAct

Notes

STAB2

Q8WWQ8

3

EBI-7946048,EBI-7945957

From Homo sapiens.

GO - Molecular functionⁱ

actin monomer binding Source: InterPro

Complete GO annotation...

Protein-protein interaction databases

BioGridⁱ	202471. 9 interactors.
IntActⁱ	P20065. 1 interactor.
Molecular INTeraction database More...MINTⁱ	MINT-6542333.
STRINGⁱ	10090.ENSMUSP00000107795.

Structureⁱ

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1T44	X-ray	2.00	G	28-46	[»]
	3M3N	X-ray	7.00	W	26-50	[»]
ProteinModelPortalⁱ	P20065.
SMRⁱ	P20065.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P20065.

EvolutionaryTraceⁱ

P20065.

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the thymosin beta family.Curated

Phylogenomic databases

eggNOGⁱ	KOG4794. Eukaryota. ENOG410Y3I4. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000007040.
HOGENOMⁱ	HOG000231370.
HOVERGENⁱ	HBG012534.
InParanoidⁱ	P20065.
KEGG Orthology (KO) More...KOⁱ	K05764.
OMAⁱ	SKETIGQ.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G1AVI.
PhylomeDBⁱ	P20065.

Family and domain databases

InterProⁱ	View protein in InterPro IPR001152. Beta-thymosin.
PANTHERⁱ	PTHR12021. PTHR12021. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01290. Thymosin. 1 hit.
PIRSFⁱ	PIRSF001828. Thymosin_beta. 1 hit.
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD005116. Thymosin_b4. 1 hit.
SMARTⁱ	View protein in SMART SM00152. THY. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00500. THYMOSIN_B4. 1 hit.

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket Added to basket

Isoform Long (identifier: P20065-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLPATMSDK PDMAEIEKFD KSKLKKTETQ EKNPLPSKET IEQEKQAGES

Length:50

Mass (Da):5,679

Last modified:February 1, 1991 - v1

Checksum:ⁱ9A289F60EE48EB8A

GO

Isoform Short (identifier: P20065-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-6: Missing.

« Hide

        10         20         30         40 
MSDKPDMAEI EKFDKSKLKK TETQEKNPLP SKETIEQEKQ AGES

Show »

Length:44

Mass (Da):5,053

Checksum:ⁱ440C6158482DAAD0

GO

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_006424	1 – 6	Missing in isoform Short. Curated		6

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X16053 mRNA. Translation: CAA34187.1. X16053 mRNA. Translation: CAA34188.1. U38967 Genomic DNA. Translation: AAC52490.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS30529.1. [P20065-2]
PIRⁱ	A37217.
NCBI Reference Sequences More...RefSeqⁱ	NP_067253.1. NM_021278.2. [P20065-2] XP_006528822.1. XM_006528759.1. [P20065-2]
UniGeneⁱ	Mm.142729. Mm.490263.

Genome annotation databases

Ensemblⁱ	ENSMUST00000112172; ENSMUSP00000107795; ENSMUSG00000049775. [P20065-2] ENSMUST00000112175; ENSMUSP00000107797; ENSMUSG00000049775. [P20065-1] ENSMUST00000112176; ENSMUSP00000107798; ENSMUSG00000049775. [P20065-1]
GeneIDⁱ	19241.
KEGGⁱ	mmu:19241.
UCSC genome browser More...UCSCⁱ	uc009uwx.1. mouse. [P20065-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X16053 mRNA. Translation: CAA34187.1. X16053 mRNA. Translation: CAA34188.1. U38967 Genomic DNA. Translation: AAC52490.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS30529.1. [P20065-2]
PIRⁱ	A37217.
NCBI Reference Sequences More...RefSeqⁱ	NP_067253.1. NM_021278.2. [P20065-2] XP_006528822.1. XM_006528759.1. [P20065-2]
UniGeneⁱ	Mm.142729. Mm.490263.

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1T44	X-ray	2.00	G	28-46	[»]
	3M3N	X-ray	7.00	W	26-50	[»]
ProteinModelPortalⁱ	P20065.
SMRⁱ	P20065.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	202471. 9 interactors.
IntActⁱ	P20065. 1 interactor.
Molecular INTeraction database More...MINTⁱ	MINT-6542333.
STRINGⁱ	10090.ENSMUSP00000107795.

PTM databases

iPTMnetⁱ	P20065.
PhosphoSitePlusⁱ	P20065.

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P20065.
PaxDbⁱ	P20065.
PeptideAtlas More...PeptideAtlasⁱ	P20065.
PRIDEⁱ	P20065.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000112172; ENSMUSP00000107795; ENSMUSG00000049775. [P20065-2] ENSMUST00000112175; ENSMUSP00000107797; ENSMUSG00000049775. [P20065-1] ENSMUST00000112176; ENSMUSP00000107798; ENSMUSG00000049775. [P20065-1]
GeneIDⁱ	19241.
KEGGⁱ	mmu:19241.
UCSC genome browser More...UCSCⁱ	uc009uwx.1. mouse. [P20065-1]

Organism-specific databases

CTDⁱ	7114.
MGIⁱ	MGI:99510. Tmsb4x.

Phylogenomic databases

eggNOGⁱ	KOG4794. Eukaryota. ENOG410Y3I4. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000007040.
HOGENOMⁱ	HOG000231370.
HOVERGENⁱ	HBG012534.
InParanoidⁱ	P20065.
KEGG Orthology (KO) More...KOⁱ	K05764.
OMAⁱ	SKETIGQ.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G1AVI.
PhylomeDBⁱ	P20065.

Enzyme and pathway databases

Reactomeⁱ	R-MMU-114608. Platelet degranulation.

Reactomeⁱ

R-MMU-114608. Platelet degranulation.

Miscellaneous databases

ChiTaRSⁱ	Tmsb4x. mouse.
EvolutionaryTraceⁱ	P20065.
Protein Ontology More...PROⁱ	PR:P20065.
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000049775.
CleanExⁱ	MM_TMSB4X.
ExpressionAtlasⁱ	P20065. baseline and differential.
Genevisibleⁱ	P20065. MM.

Family and domain databases

InterProⁱ	View protein in InterPro IPR001152. Beta-thymosin.
PANTHERⁱ	PTHR12021. PTHR12021. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01290. Thymosin. 1 hit.
PIRSFⁱ	PIRSF001828. Thymosin_beta. 1 hit.
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD005116. Thymosin_b4. 1 hit.
SMARTⁱ	View protein in SMART SM00152. THY. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00500. THYMOSIN_B4. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TYB4_MOUSE
Accessionⁱ	P20065Primary (citable) accession number: P20065
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
	Last sequence update:	February 1, 1991
	Last modified:	July 5, 2017
	This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P20065 (TYB4_MOUSE)

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Thymosin beta-4

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2)i

Alternative sequence

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequences (2)ⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ