P20065 (TYB4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymosin beta-4
Short name=T beta 4

Cleaved into the following chain:

1. Hematopoietic system regulatory peptide
   Alternative name(s):
   Seraspenide

Gene names

Name:	Tmsb4x
Synonyms:	Ptmb4, Tmsb4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	50 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells into the S-phase By similarity.
Subcellular location	Cytoplasm › cytoskeleton.
Tissue specificity	Originally found in thymus but it is widely distributed in many tissues.
Sequence similarities	Belongs to the thymosin beta family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing
Ligand	Actin-binding
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	actin cytoskeleton organization Inferred from electronic annotation. Source: InterPro positive regulation of smooth muscle cell differentiation Traceable author statement. Source: DFLAT positive regulation of smooth muscle cell migration Traceable author statement. Source: DFLAT positive regulation of transcription, DNA-dependent Traceable author statement. Source: DFLAT sequestering of actin monomers Inferred from electronic annotation. Source: InterPro
Cellular component	cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from direct assay. Source: MGI nucleus Inferred from direct assay. Source: MGI
Molecular function	actin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P20065-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P20065-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Peptide

1 – 50

50

Thymosin beta-4

PRO_0000045923

Peptide

8 – 11

4

Hematopoietic system regulatory peptide By similarity

PRO_0000034297

Amino acid modifications

Modified residue

8

1

N-acetylserine By similarity

Modified residue

10

1

N6-acetyllysine By similarity

Modified residue

18

1

N6-acetyllysine By similarity

Modified residue

29

1

Phosphothreonine By similarity

Modified residue

32

1

N6-acetyllysine By similarity

Modified residue

38

1

N6-acetyllysine By similarity

Modified residue

45

1

N6-acetyllysine By similarity

Natural variations

Alternative sequence

1 – 6

6

Missing in isoform Short.

VSP_006424

Secondary structure

1

.

.

.

50

Helix Strand Turn

Details...

Helix

38 – 45

8

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 9A289F60EE48EB8A	FASTA	50	5,679
	10 20 30 40 50 MLLPATMSDK PDMAEIEKFD KSKLKKTETQ EKNPLPSKET IEQEKQAGES
	Isoform Short [UniParc]. Checksum: 440C6158482DAAD0	FASTA	44	5,053
	10 20 30 40 MSDKPDMAEI EKFDKSKLKK TETQEKNPLP SKETIEQEKQ AGES

References

[1]	"Differential splicing of thymosin beta 4 mRNA." Rudin C.M., Engler P., Storb U. J. Immunol. 144:4857-4862(1990) [PubMed: 2351831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
[2]	"Depression of prothymosin alpha production in murine thymus correlates with staphylococcal enterotoxin-B-induced immunosuppression." Low T.L.K., Pan T.L., Lin Y.S. FEBS Lett. 273:1-5(1990) [PubMed: 2226839] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM SHORT).
[3]	"The mouse thymosin beta 4 gene: structure, promoter identification, and chromosome localization." Li X., Zimmerman A., Copeland N.G., Gilbert D.J., Jenkins N.A., Yin H.L. Genomics 32:388-394(1996) [PubMed: 8838802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT). Strain: 129/Sv.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X16053 mRNA. Translation: CAA34187.1.
X16053 mRNA. Translation: CAA34188.1.
U38967 Genomic DNA. Translation: AAC52490.1.

IPI

IPI00228757.
IPI00853896.

PIR

A37217.

RefSeq

NP_067253.1. NM_021278.2.

UniGene

Mm.142729.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1T44	X-ray	2.00	G	28-46	[»]
3M3N	X-ray	7.00	W	26-50	[»]

ProteinModelPortal

P20065.

SMR

P20065. Positions 8-48.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-6542333.

STRING

P20065.

PTM databases

PhosphoSite

P20065.

Proteomic databases

PRIDE

P20065.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000112172; ENSMUSP00000107795; ENSMUSG00000049775.
ENSMUST00000112175; ENSMUSP00000107797; ENSMUSG00000049775.
ENSMUST00000112176; ENSMUSP00000107798; ENSMUSG00000049775.

GeneID

19241.

KEGG

mmu:19241.

Organism-specific databases

CTD

7114.

MGI

MGI:99510. Tmsb4x.

Phylogenomic databases

HOGENOM

HBG444190.

HOVERGEN

HBG012534.

InParanoid

P20065.

OMA

SCASLRF.

OrthoDB

EOG4HDSWB.

PhylomeDB

P20065.

Gene expression databases

ArrayExpress

P20065.

Bgee

P20065.

CleanEx

MM_TMSB4X.

Genevestigator

P20065.

GermOnline

ENSMUSG00000049775. Mus musculus.

Family and domain databases

InterPro

IPR001152. Thymosin_b4.
IPR016323. Thymosin_b4_chordata.
[Graphical view]

Gene3D

G3DSA:1.20.5.520. Thymosin_b4. 1 hit.

KO

K05764.

PANTHER

PTHR12021. PTHR12021. 1 hit.

Pfam

PF01290. Thymosin. 1 hit.
[Graphical view]

PIRSF

PIRSF001828. Thymosin_beta. 1 hit.

ProDom

PD005116. Thymosin_b4. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00152. THY. 1 hit.
[Graphical view]

PROSITE

PS00500. THYMOSIN_B4. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

SOURCE

Search...

Entry information

Entry name

TYB4_MOUSE

Accession

Primary (citable) accession number: P20065

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	November 16, 2011
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program