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Protein

Thymosin beta-4

Gene

Tmsb4x

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.
Seraspenide inhibits the entry of hematopoietic pluripotent stem cells into the S-phase.By similarity

GO - Molecular functioni

GO - Biological processi

  • actin filament organization Source: InterPro
  • osteoblast differentiation Source: Ensembl
  • positive regulation of smooth muscle cell differentiation Source: DFLAT
  • positive regulation of smooth muscle cell migration Source: DFLAT
  • positive regulation of transcription, DNA-templated Source: DFLAT
  • regulation of cell migration Source: MGI
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymosin beta-4
Short name:
T beta 4
Cleaved into the following chain:
Alternative name(s):
Seraspenide
Gene namesi
Name:Tmsb4x
Synonyms:Ptmb4, Tmsb4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:99510. Tmsb4x.

Subcellular locationi

GO - Cellular componenti

  • cytoskeleton Source: UniProtKB-SubCell
  • cytosol Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5050Thymosin beta-4PRO_0000045923Add
BLAST
Peptidei8 – 114Hematopoietic system regulatory peptideBy similarityPRO_0000034297

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81N-acetylserineBy similarity
Modified residuei8 – 81PhosphoserineBy similarity
Modified residuei10 – 101N6-acetyllysineBy similarity
Modified residuei18 – 181N6-acetyllysineBy similarity
Modified residuei29 – 291PhosphothreonineCombined sources
Modified residuei32 – 321N6-acetyllysineBy similarity
Modified residuei38 – 381N6-acetyllysineBy similarity
Modified residuei40 – 401PhosphothreonineBy similarity
Modified residuei45 – 451N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP20065.
MaxQBiP20065.
PaxDbiP20065.
PRIDEiP20065.

PTM databases

iPTMnetiP20065.
PhosphoSiteiP20065.

Expressioni

Tissue specificityi

Originally found in thymus but it is widely distributed in many tissues.

Gene expression databases

BgeeiP20065.
CleanExiMM_TMSB4X.
ExpressionAtlasiP20065. baseline and differential.
GenevisibleiP20065. MM.

Interactioni

Subunit structurei

Interacts with SERPINB1. Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
STAB2Q8WWQ83EBI-7946048,EBI-7945957From a different organism.

Protein-protein interaction databases

BioGridi202471. 9 interactions.
IntActiP20065. 1 interaction.
MINTiMINT-6542333.
STRINGi10090.ENSMUSP00000107795.

Structurei

Secondary structure

1
50
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi38 – 458

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T44X-ray2.00G28-46[»]
3M3NX-ray7.00W26-50[»]
ProteinModelPortaliP20065.
SMRiP20065. Positions 12-47.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20065.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymosin beta family.Curated

Phylogenomic databases

eggNOGiKOG4794. Eukaryota.
ENOG410Y3I4. LUCA.
GeneTreeiENSGT00390000007040.
HOGENOMiHOG000231370.
HOVERGENiHBG012534.
InParanoidiP20065.
KOiK05764.
OMAiCASLRFS.
OrthoDBiEOG7TJ3MT.
PhylomeDBiP20065.

Family and domain databases

Gene3Di1.20.5.520. 1 hit.
InterProiIPR001152. Beta-thymosin.
[Graphical view]
PANTHERiPTHR12021. PTHR12021. 1 hit.
PfamiPF01290. Thymosin. 1 hit.
[Graphical view]
PIRSFiPIRSF001828. Thymosin_beta. 1 hit.
ProDomiPD005116. Thymosin_b4. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00152. THY. 1 hit.
[Graphical view]
PROSITEiPS00500. THYMOSIN_B4. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P20065-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLPATMSDK PDMAEIEKFD KSKLKKTETQ EKNPLPSKET IEQEKQAGES
Length:50
Mass (Da):5,679
Last modified:February 1, 1991 - v1
Checksum:i9A289F60EE48EB8A
GO
Isoform Short (identifier: P20065-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: Missing.

Show »
Length:44
Mass (Da):5,053
Checksum:i440C6158482DAAD0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 66Missing in isoform Short. CuratedVSP_006424

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16053 mRNA. Translation: CAA34187.1.
X16053 mRNA. Translation: CAA34188.1.
U38967 Genomic DNA. Translation: AAC52490.1.
CCDSiCCDS30529.1. [P20065-2]
PIRiA37217.
RefSeqiNP_067253.1. NM_021278.2. [P20065-2]
XP_006528822.1. XM_006528759.1. [P20065-2]
UniGeneiMm.142729.
Mm.490263.

Genome annotation databases

EnsembliENSMUST00000112172; ENSMUSP00000107795; ENSMUSG00000049775. [P20065-2]
ENSMUST00000112175; ENSMUSP00000107797; ENSMUSG00000049775. [P20065-1]
ENSMUST00000112176; ENSMUSP00000107798; ENSMUSG00000049775. [P20065-1]
GeneIDi19241.
KEGGimmu:19241.
UCSCiuc009uwx.1. mouse. [P20065-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16053 mRNA. Translation: CAA34187.1.
X16053 mRNA. Translation: CAA34188.1.
U38967 Genomic DNA. Translation: AAC52490.1.
CCDSiCCDS30529.1. [P20065-2]
PIRiA37217.
RefSeqiNP_067253.1. NM_021278.2. [P20065-2]
XP_006528822.1. XM_006528759.1. [P20065-2]
UniGeneiMm.142729.
Mm.490263.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T44X-ray2.00G28-46[»]
3M3NX-ray7.00W26-50[»]
ProteinModelPortaliP20065.
SMRiP20065. Positions 12-47.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202471. 9 interactions.
IntActiP20065. 1 interaction.
MINTiMINT-6542333.
STRINGi10090.ENSMUSP00000107795.

PTM databases

iPTMnetiP20065.
PhosphoSiteiP20065.

Proteomic databases

EPDiP20065.
MaxQBiP20065.
PaxDbiP20065.
PRIDEiP20065.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000112172; ENSMUSP00000107795; ENSMUSG00000049775. [P20065-2]
ENSMUST00000112175; ENSMUSP00000107797; ENSMUSG00000049775. [P20065-1]
ENSMUST00000112176; ENSMUSP00000107798; ENSMUSG00000049775. [P20065-1]
GeneIDi19241.
KEGGimmu:19241.
UCSCiuc009uwx.1. mouse. [P20065-1]

Organism-specific databases

CTDi7114.
MGIiMGI:99510. Tmsb4x.

Phylogenomic databases

eggNOGiKOG4794. Eukaryota.
ENOG410Y3I4. LUCA.
GeneTreeiENSGT00390000007040.
HOGENOMiHOG000231370.
HOVERGENiHBG012534.
InParanoidiP20065.
KOiK05764.
OMAiCASLRFS.
OrthoDBiEOG7TJ3MT.
PhylomeDBiP20065.

Miscellaneous databases

ChiTaRSiTmsb4x. mouse.
EvolutionaryTraceiP20065.
NextBioi296050.
PROiP20065.
SOURCEiSearch...

Gene expression databases

BgeeiP20065.
CleanExiMM_TMSB4X.
ExpressionAtlasiP20065. baseline and differential.
GenevisibleiP20065. MM.

Family and domain databases

Gene3Di1.20.5.520. 1 hit.
InterProiIPR001152. Beta-thymosin.
[Graphical view]
PANTHERiPTHR12021. PTHR12021. 1 hit.
PfamiPF01290. Thymosin. 1 hit.
[Graphical view]
PIRSFiPIRSF001828. Thymosin_beta. 1 hit.
ProDomiPD005116. Thymosin_b4. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00152. THY. 1 hit.
[Graphical view]
PROSITEiPS00500. THYMOSIN_B4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Differential splicing of thymosin beta 4 mRNA."
    Rudin C.M., Engler P., Storb U.
    J. Immunol. 144:4857-4862(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
  2. "Depression of prothymosin alpha production in murine thymus correlates with staphylococcal enterotoxin-B-induced immunosuppression."
    Low T.L.K., Pan T.L., Lin Y.S.
    FEBS Lett. 273:1-5(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM SHORT).
  3. "The mouse thymosin beta 4 gene: structure, promoter identification, and chromosome localization."
    Li X., Zimmerman A., Copeland N.G., Gilbert D.J., Jenkins N.A., Yin H.L.
    Genomics 32:388-394(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
    Strain: 129/Sv.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-29, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Kidney, Lung, Pancreas and Spleen.

Entry informationi

Entry nameiTYB4_MOUSE
AccessioniPrimary (citable) accession number: P20065
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: May 11, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.