Tmsb4x

Functionⁱ

Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.

Seraspenide inhibits the entry of hematopoietic pluripotent stem cells into the S-phase.By similarity

GO - Molecular functionⁱ

actin monomer binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

actin filament organization Source: InterPro
osteoblast differentiation Source: Ensembl
positive regulation of smooth muscle cell differentiation
Source: DFLAT
Traceable author statementⁱ
- 20299672
positive regulation of smooth muscle cell migration
Source: DFLAT
Traceable author statementⁱ
- 20299672
positive regulation of transcription, DNA-templated
Source: DFLAT
Traceable author statementⁱ
- 20299672
regulation of cell migration
Source: MGI
Inferred from direct assayⁱ
- 15565145

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Actin-binding

Molecular function

Actin-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Thymosin beta-4 Short name: T beta 4 Cleaved into the following chain: Hematopoietic system regulatory peptide Alternative name(s): Seraspenide
Gene namesⁱ	Name:Tmsb4x Synonyms:Ptmb4, Tmsb4
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome X

Organism-specific databases

MGIⁱ	MGI:99510 Tmsb4x

Keywords - Cellular componentⁱ

Cytoplasm, Cytoskeleton

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000045923	1 – 50	Thymosin beta-4Add BLAST		50
PeptideⁱPRO_0000034297	8 – 11	Hematopoietic system regulatory peptideBy similarity		4

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	8	PhosphoserineBy similarity	1
Modified residueⁱ	10	N6-acetyllysineBy similarity	1
Modified residueⁱ	18	N6-acetyllysine; alternateBy similarity	1
Cross-linkⁱ	18	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residueⁱ	29	PhosphothreonineCombined sources	1
Modified residueⁱ	32	N6-acetyllysineBy similarity	1
Modified residueⁱ	37	PhosphoserineBy similarity	1
Modified residueⁱ	38	N6-acetyllysineBy similarity	1
Modified residueⁱ	40	PhosphothreonineBy similarity	1
Modified residueⁱ	45	N6-acetyllysineBy similarity	1

Keywords - PTMⁱ

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P20065
PaxDbⁱ	P20065
PeptideAtlas More...PeptideAtlasⁱ	P20065
PRIDEⁱ	P20065

PTM databases

iPTMnetⁱ	P20065
PhosphoSitePlusⁱ	P20065

Expressionⁱ

Tissue specificityⁱ

Originally found in thymus but it is widely distributed in many tissues.

Gene expression databases

Bgeeⁱ	ENSMUSG00000049775
CleanExⁱ	MM_TMSB4X
ExpressionAtlasⁱ	P20065 baseline and differential
Genevisibleⁱ	P20065 MM

Interactionⁱ

Subunit structureⁱ

Interacts with SERPINB1. Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X (By similarity).By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
STAB2	Q8WWQ8	3	EBI-7946048,EBI-7945957	From Homo sapiens.

With

Entry

#Exp.

IntAct

Notes

STAB2

Q8WWQ8

3

EBI-7946048,EBI-7945957

From Homo sapiens.

GO - Molecular functionⁱ

actin monomer binding Source: InterPro

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	2024719 interactors.
CORUMⁱ	P20065
ELMⁱ	P20065
IntActⁱ	P20065 1 interactor.
Molecular INTeraction database More...MINTⁱ	P20065
STRINGⁱ	10090.ENSMUSP00000107795

Structureⁱ

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1T44	X-ray	2.00	G	28-46	[»]
	3M3N	X-ray	7.00	W	26-50	[»]
ProteinModelPortalⁱ	P20065
SMRⁱ	P20065
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P20065

EvolutionaryTraceⁱ

P20065

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the thymosin beta family.Curated

Phylogenomic databases

eggNOGⁱ	KOG4794 Eukaryota ENOG410Y3I4 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000007040
HOGENOMⁱ	HOG000231370
HOVERGENⁱ	HBG012534
InParanoidⁱ	P20065
KEGG Orthology (KO) More...KOⁱ	K05764
OMAⁱ	SKETIGQ
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G1AVI
PhylomeDBⁱ	P20065

Family and domain databases

Gene3Dⁱ	1.20.5.5201 hit
InterProⁱ	View protein in InterPro IPR001152 Beta-thymosin IPR038386 Beta-thymosin_sf
PANTHERⁱ	PTHR12021 PTHR12021, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01290 Thymosin, 1 hit
PIRSFⁱ	PIRSF001828 Thymosin_beta, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD005116 Thymosin_b4, 1 hit
SMARTⁱ	View protein in SMART SM00152 THY, 1 hit
PROSITEⁱ	View protein in PROSITE PS00500 THYMOSIN_B4, 1 hit

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket Added to basket

Isoform Long (identifier: P20065-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLPATMSDK PDMAEIEKFD KSKLKKTETQ EKNPLPSKET IEQEKQAGES

Length:50

Mass (Da):5,679

Last modified:February 1, 1991 - v1

Checksum:ⁱ9A289F60EE48EB8A

GO

Isoform Short (identifier: P20065-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-6: Missing.

« Hide

        10         20         30         40 
MSDKPDMAEI EKFDKSKLKK TETQEKNPLP SKETIEQEKQ AGES

Show »

Length:44

Mass (Da):5,053

Checksum:ⁱ440C6158482DAAD0

GO

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_006424	1 – 6	Missing in isoform Short. Curated		6

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X16053 mRNA Translation: CAA34187.1 X16053 mRNA Translation: CAA34188.1 U38967 Genomic DNA Translation: AAC52490.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS30529.1 [P20065-2]
PIRⁱ	A37217
NCBI Reference Sequences More...RefSeqⁱ	NP_067253.1, NM_021278.2 [P20065-2] XP_006528822.1, XM_006528759.1 [P20065-2]
UniGeneⁱ	Mm.142729 Mm.490263

Genome annotation databases

Ensemblⁱ	ENSMUST00000112172; ENSMUSP00000107795; ENSMUSG00000049775 [P20065-2] ENSMUST00000112175; ENSMUSP00000107797; ENSMUSG00000049775 [P20065-1] ENSMUST00000112176; ENSMUSP00000107798; ENSMUSG00000049775 [P20065-1]
GeneIDⁱ	19241
KEGGⁱ	mmu:19241
UCSC genome browser More...UCSCⁱ	uc009uwx.1 mouse [P20065-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P20065	UPI0005FB492D UPI0004F03398 A0A2K5MGS8	Ovis aries (Sheep) Callithrix jacchus (White-tufted-ear marmoset) Cercocebus atys (Sooty mangabey) (Cercocebus torquatus atys)	50	UniRef90_P20065	Cluster: Thymosin beta-4	4
P20065-2	P62328 P62326 Q6S9C5 P62327 Q95274 Q5R7H8 P62329 A2VCK8 A0A2K5DFU2 F7HV51 +59	Homo sapiens (Human) Bos taurus (Bovine) Chinchilla lanigera (Long-tailed chinchilla) (Chinchilla villidera) Equus caballus (Horse) Sus scrofa (Pig) Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) Rattus norvegicus (Rat) Aotus nancymaae (Ma's night monkey) Callithrix jacchus (White-tufted-ear marmoset) Canis lupus familiaris (Dog) (Canis familiaris) And more	44	UniRef90_P62328	Cluster: Thymosin beta-4	70

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P20065	UPI0005FB492D UPI0004F03398 A0A2K5MGS8 L5JT10 UPI00067E4841	Ovis aries (Sheep) Callithrix jacchus (White-tufted-ear marmoset) Cercocebus atys (Sooty mangabey) (Cercocebus torquatus atys) Pteropus alecto (Black flying fox) Microtus ochrogaster (Prairie vole)	50	UniRef50_P20065	Cluster: Thymosin beta-4	6
P20065-2	P62328 P62326 Q6S9C5 P62327 Q95274 Q5R7H8 P62329 A2VCK8 A0A2K5DFU2 F7HV51 +59	Homo sapiens (Human) Bos taurus (Bovine) Chinchilla lanigera (Long-tailed chinchilla) (Chinchilla villidera) Equus caballus (Horse) Sus scrofa (Pig) Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) Rattus norvegicus (Rat) Aotus nancymaae (Ma's night monkey) Callithrix jacchus (White-tufted-ear marmoset) Canis lupus familiaris (Dog) (Canis familiaris) And more	44	UniRef50_P62328	Cluster: Thymosin beta-4	70

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X16053 mRNA Translation: CAA34187.1 X16053 mRNA Translation: CAA34188.1 U38967 Genomic DNA Translation: AAC52490.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS30529.1 [P20065-2]
PIRⁱ	A37217
NCBI Reference Sequences More...RefSeqⁱ	NP_067253.1, NM_021278.2 [P20065-2] XP_006528822.1, XM_006528759.1 [P20065-2]
UniGeneⁱ	Mm.142729 Mm.490263

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1T44	X-ray	2.00	G	28-46	[»]
	3M3N	X-ray	7.00	W	26-50	[»]
ProteinModelPortalⁱ	P20065
SMRⁱ	P20065
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	2024719 interactors.
CORUMⁱ	P20065
ELMⁱ	P20065
IntActⁱ	P20065 1 interactor.
Molecular INTeraction database More...MINTⁱ	P20065
STRINGⁱ	10090.ENSMUSP00000107795

PTM databases

iPTMnetⁱ	P20065
PhosphoSitePlusⁱ	P20065

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P20065
PaxDbⁱ	P20065
PeptideAtlas More...PeptideAtlasⁱ	P20065
PRIDEⁱ	P20065

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000112172; ENSMUSP00000107795; ENSMUSG00000049775 [P20065-2] ENSMUST00000112175; ENSMUSP00000107797; ENSMUSG00000049775 [P20065-1] ENSMUST00000112176; ENSMUSP00000107798; ENSMUSG00000049775 [P20065-1]
GeneIDⁱ	19241
KEGGⁱ	mmu:19241
UCSC genome browser More...UCSCⁱ	uc009uwx.1 mouse [P20065-1]

Organism-specific databases

CTDⁱ	7114
MGIⁱ	MGI:99510 Tmsb4x

Phylogenomic databases

eggNOGⁱ	KOG4794 Eukaryota ENOG410Y3I4 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000007040
HOGENOMⁱ	HOG000231370
HOVERGENⁱ	HBG012534
InParanoidⁱ	P20065
KEGG Orthology (KO) More...KOⁱ	K05764
OMAⁱ	SKETIGQ
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G1AVI
PhylomeDBⁱ	P20065

Miscellaneous databases

ChiTaRSⁱ	Tmsb4x mouse
EvolutionaryTraceⁱ	P20065
Protein Ontology More...PROⁱ	PR:P20065
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000049775
CleanExⁱ	MM_TMSB4X
ExpressionAtlasⁱ	P20065 baseline and differential
Genevisibleⁱ	P20065 MM

Family and domain databases

Gene3Dⁱ	1.20.5.5201 hit
InterProⁱ	View protein in InterPro IPR001152 Beta-thymosin IPR038386 Beta-thymosin_sf
PANTHERⁱ	PTHR12021 PTHR12021, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01290 Thymosin, 1 hit
PIRSFⁱ	PIRSF001828 Thymosin_beta, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD005116 Thymosin_b4, 1 hit
SMARTⁱ	View protein in SMART SM00152 THY, 1 hit
PROSITEⁱ	View protein in PROSITE PS00500 THYMOSIN_B4, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TYB4_MOUSE
Accessionⁱ	P20065Primary (citable) accession number: P20065
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
	Last sequence update:	February 1, 1991
	Last modified:	April 25, 2018
	This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P20065 (TYB4_MOUSE)

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Thymosin beta-4

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytoskeleton

Cytoskeleton

Cytosol

Extracellular region or secreted

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Alternative sequence

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ