P20061 (TCO1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcobalamin-1
Short name=TC-1

Alternative name(s):
Transcobalamin I
Short name=TC I
Short name=TCI

Gene names

Name:	TCN1
Synonyms:	TC1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Vitamin B12-binding protein. Transports cobalamin into cells.
Subcellular location	Secreted.
Tissue specificity	Neutrophil granule protein.
Post-translational modification	Contains about 30% carbohydrates.
Sequence similarities	Belongs to the eukaryotic cobalamin transport proteins family.

Ontologies

Keywords
Biological process	Cobalt transport Ion transport Transport
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Ligand	Cobalt
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cobalamin metabolic process Inferred from Biological aspect of Ancestor. Source: RefGenome cobalamin transport Traceable author statement Ref.1. Source: ProtInc cobalt ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cobalamin binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23
	Chain	24 – 433	410	Transcobalamin-1		PRO_0000005561
Regions
	Region	142 – 146	5	Cobalamin binding By similarity
	Region	182 – 186	5	Cobalamin binding By similarity
	Region	402 – 404	3	Cobalamin binding By similarity
Sites
	Binding site	240	1	Cobalamin By similarity
	Binding site	243	1	Cobalamin By similarity
	Binding site	289	1	Cobalamin By similarity
Amino acid modifications
	Glycosylation	160	1	N-linked (GlcNAc...) Potential
	Glycosylation	216	1	N-linked (GlcNAc...) Ref.5 Ref.6
	Glycosylation	316	1	N-linked (GlcNAc...) Potential
	Glycosylation	337	1	N-linked (GlcNAc...) Potential
	Glycosylation	343	1	N-linked (GlcNAc...) Potential
	Glycosylation	349	1	N-linked (GlcNAc...) Potential
	Glycosylation	354	1	N-linked (GlcNAc...) Potential
	Glycosylation	369	1	N-linked (GlcNAc...) Ref.6
	Disulfide bond	26 ↔ 265		By similarity
	Disulfide bond	105 ↔ 308		By similarity
	Disulfide bond	155 ↔ 197		By similarity
Natural variations
	Natural variant	35	1	R → H. Corresponds to variant rs34528912 [ dbSNP | Ensembl ].		VAR_031923
	Natural variant	301	1	D → Y. Corresponds to variant rs34324219 [ dbSNP | Ensembl ].		VAR_031924
Experimental info
	Sequence conflict	119	1	I → T in AAA61058. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P20061 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: CF1052BE3DBA929A	FASTA	433	48,207
	10 20 30 40 50 60 MRQSHQLPLV GLLLFSFIPS QLCEICEVSE ENYIRLKPLL NTMIQSNYNR GTSAVNVVLS 70 80 90 100 110 120 LKLVGIQIQT LMQKMIQQIK YNVKSRLSDV SSGELALIIL ALGVCRNAEE NLIYDYHLID 130 140 150 160 170 180 KLENKFQAEI ENMEAHNGTP LTNYYQLSLD VLALCLFNGN YSTAEVVNHF TPENKNYYFG 190 200 210 220 230 240 SQFSVDTGAM AVLALTCVKK SLINGQIKAD EGSLKNISIY TKSLVEKILS EKKENGLIGN 250 260 270 280 290 300 TFSTGEAMQA LFVSSDYYNE NDWNCQQTLN TVLTEISQGA FSNPNAAAQV LPALMGKTFL 310 320 330 340 350 360 DINKDSSCVS ASGNFNISAD EPITVTPPDS QSYISVNYSV RINETYFTNV TVLNGSVFLS 370 380 390 400 410 420 VMEKAQKMND TIFGFTMEER SWGPYITCIQ GLCANNNDRT YWELLSGGEP LSQGAGSYVV 430 RNGENLEVRW SKY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the cDNA encoding transcobalamin I, a neutrophil granule protein." Johnston J., Bollekens J., Allen R.H., Berliner N. J. Biol. Chem. 264:15754-15757(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Trachea.
[3]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-216, MASS SPECTROMETRY. Tissue: Plasma.
[6]	"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry." Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A. J. Proteome Res. 5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-216 AND ASN-369, MASS SPECTROMETRY. Tissue: Saliva.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J05068 mRNA. Translation: AAA61058.1.
AK292990 mRNA. Translation: BAF85679.1.
AP002347 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73861.1.

IPI

IPI00299729.

PIR

A34227.

RefSeq

NP_001053.2. NM_001062.3.

UniGene

Hs.2012.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CKV	model	-	Y	24-433	[»]

ProteinModelPortal

P20061.

ModBase

Search...

Protein-protein interaction databases

IntAct

P20061. 1 interaction.

STRING

9606.ENSP00000257264.

PTM databases

PhosphoSite

P20061.

Polymorphism databases

DMDM

146345530.

Proteomic databases

PaxDb

P20061.

PRIDE

P20061.

Protocols and materials databases

DNASU

6947.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000257264; ENSP00000257264; ENSG00000134827.

GeneID

6947.

KEGG

hsa:6947.

UCSC

uc001noj.2. human.

Organism-specific databases

CTD

6947.

GeneCards

GC11M059620.

H-InvDB

HIX0009662.

HGNC

HGNC:11652. TCN1.

HPA

HPA041631.

MIM

189905. gene.

neXtProt

NX_P20061.

PharmGKB

PA36403.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG79743.

HOGENOM

HOG000013214.

HOVERGEN

HBG094393.

InParanoid

P20061.

OMA

ENLEVRW.

OrthoDB

EOG4V437P.

PhylomeDB

P20061.

Gene expression databases

Bgee

P20061.

CleanEx

HS_TCN1.

Genevestigator

P20061.

GermOnline

ENSG00000134827. Homo sapiens.

Family and domain databases

InterPro

IPR002157. Cbl-bd_transpt_euk.
[Graphical view]

PANTHER

PTHR10559. PTHR10559. 1 hit.

Pfam

PF01122. Cobalamin_bind. 1 hit.
[Graphical view]

PROSITE

PS00468. COBALAMIN_BINDING. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB00115. Cyanocobalamin.
DB00200. Hydroxocobalamin.

GenomeRNAi

6947.

NextBio

27199.

SOURCE

Search...

Entry information

Entry name

TCO1_HUMAN

Accession

Primary (citable) accession number: P20061
Secondary accession number(s): A8KAC5, Q8WV77

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	May 1, 2007
Last modified:	May 1, 2013
This is version 121 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.