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Protein

Transcobalamin-1

Gene

TCN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds vitamin B12 with femtomolar affinity and protects it from the acidic environment of the stomach.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei240Cobalamin1
Binding sitei243Cobalamin1
Binding sitei289Cobalamin1
Binding sitei433Cobalamin1

GO - Molecular functioni

GO - Biological processi

  • cobalamin metabolic process Source: Reactome
  • cobalamin transport Source: ProtInc
  • cobalt ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cobalt transport, Ion transport, Transport

Keywords - Ligandi

Cobalt

Enzyme and pathway databases

BioCyciZFISH:ENSG00000134827-MONOMER.
ReactomeiR-HSA-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.
R-HSA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcobalamin-1
Short name:
TC-1
Alternative name(s):
Haptocorrin
Short name:
HC
Protein R
Transcobalamin I
Short name:
TC I
Short name:
TCI
Gene namesi
Name:TCN1
Synonyms:TC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:11652. TCN1.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: Reactome
  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi6947.
OpenTargetsiENSG00000134827.
PharmGKBiPA36403.

Chemistry databases

DrugBankiDB00115. Cyanocobalamin.
DB00200. Hydroxocobalamin.

Polymorphism and mutation databases

BioMutaiTCN1.
DMDMi146345530.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Add BLAST23
ChainiPRO_000000556124 – 433Transcobalamin-1Add BLAST410

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 2651 Publication
Disulfide bondi105 ↔ 3081 Publication
Disulfide bondi155 ↔ 1971 Publication
Glycosylationi160N-linked (GlcNAc...)Sequence analysis1
Glycosylationi216N-linked (GlcNAc...)3 Publications1
Glycosylationi316N-linked (GlcNAc...)1 Publication1
Glycosylationi337N-linked (GlcNAc...)1 Publication1
Glycosylationi343N-linked (GlcNAc...)1 Publication1
Glycosylationi349N-linked (GlcNAc...)1 Publication1
Glycosylationi354N-linked (GlcNAc...)1 Publication1
Glycosylationi369N-linked (GlcNAc...)2 Publications1
Disulfide bondi388 ↔ 3931 Publication

Post-translational modificationi

Contains about 30% carbohydrates.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP20061.
PeptideAtlasiP20061.
PRIDEiP20061.

PTM databases

iPTMnetiP20061.
PhosphoSitePlusiP20061.

Expressioni

Tissue specificityi

Produced by the salivary glands of the oral cavity, in response to ingestion of food. Major constituent of secondary granules in neutrophils.1 Publication

Gene expression databases

BgeeiENSG00000134827.
CleanExiHS_TCN1.
GenevisibleiP20061. HS.

Interactioni

Protein-protein interaction databases

BioGridi112807. 3 interactors.
IntActiP20061. 1 interactor.
STRINGi9606.ENSP00000257264.

Structurei

Secondary structure

1433
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 36Combined sources7
Helixi37 – 45Combined sources9
Helixi46 – 48Combined sources3
Beta strandi50 – 52Combined sources3
Helixi55 – 63Combined sources9
Helixi69 – 85Combined sources17
Turni87 – 89Combined sources3
Helixi92 – 102Combined sources11
Helixi108 – 110Combined sources3
Helixi111 – 115Combined sources5
Helixi118 – 135Combined sources18
Turni136 – 138Combined sources3
Helixi144 – 156Combined sources13
Helixi163 – 169Combined sources7
Helixi175 – 177Combined sources3
Helixi185 – 203Combined sources19
Helixi213 – 229Combined sources17
Beta strandi238 – 240Combined sources3
Turni241 – 243Combined sources3
Helixi244 – 252Combined sources9
Helixi255 – 257Combined sources3
Turni260 – 262Combined sources3
Helixi265 – 277Combined sources13
Turni278 – 281Combined sources4
Helixi284 – 294Combined sources11
Helixi299 – 301Combined sources3
Beta strandi333 – 353Combined sources21
Helixi358 – 368Combined sources11
Helixi370 – 373Combined sources4
Beta strandi375 – 379Combined sources5
Beta strandi381 – 389Combined sources9
Turni396 – 399Combined sources4
Beta strandi400 – 406Combined sources7
Turni415 – 417Combined sources3
Beta strandi426 – 432Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CKVmodel-Y24-433[»]
4KKIX-ray2.35A1-433[»]
4KKJX-ray3.00A1-433[»]
ProteinModelPortaliP20061.
SMRiP20061.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 310Globular N-terminal alpha domainAdd BLAST287
Regioni142 – 146Cobalamin binding5
Regioni182 – 186Cobalamin binding5
Regioni311 – 332Flexible linkerAdd BLAST22
Regioni333 – 433Globular C-terminal beta domainAdd BLAST101
Regioni402 – 404Cobalamin binding3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IVBN. Eukaryota.
ENOG410XSU1. LUCA.
GeneTreeiENSGT00530000063370.
HOGENOMiHOG000013214.
HOVERGENiHBG094393.
InParanoidiP20061.
OMAiNNDRTYW.
OrthoDBiEOG091G095C.
PhylomeDBiP20061.
TreeFamiTF333092.

Family and domain databases

InterProiIPR002157. Cbl-bd_transpt_euk.
IPR027954. DUF4430.
[Graphical view]
PANTHERiPTHR10559. PTHR10559. 1 hit.
PfamiPF01122. Cobalamin_bind. 1 hit.
PF14478. DUF4430. 1 hit.
[Graphical view]
PROSITEiPS00468. COBALAMIN_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQSHQLPLV GLLLFSFIPS QLCEICEVSE ENYIRLKPLL NTMIQSNYNR
60 70 80 90 100
GTSAVNVVLS LKLVGIQIQT LMQKMIQQIK YNVKSRLSDV SSGELALIIL
110 120 130 140 150
ALGVCRNAEE NLIYDYHLID KLENKFQAEI ENMEAHNGTP LTNYYQLSLD
160 170 180 190 200
VLALCLFNGN YSTAEVVNHF TPENKNYYFG SQFSVDTGAM AVLALTCVKK
210 220 230 240 250
SLINGQIKAD EGSLKNISIY TKSLVEKILS EKKENGLIGN TFSTGEAMQA
260 270 280 290 300
LFVSSDYYNE NDWNCQQTLN TVLTEISQGA FSNPNAAAQV LPALMGKTFL
310 320 330 340 350
DINKDSSCVS ASGNFNISAD EPITVTPPDS QSYISVNYSV RINETYFTNV
360 370 380 390 400
TVLNGSVFLS VMEKAQKMND TIFGFTMEER SWGPYITCIQ GLCANNNDRT
410 420 430
YWELLSGGEP LSQGAGSYVV RNGENLEVRW SKY
Length:433
Mass (Da):48,207
Last modified:May 1, 2007 - v2
Checksum:iCF1052BE3DBA929A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119I → T in AAA61058 (PubMed:2777761).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03192335R → H.Corresponds to variant rs34528912dbSNPEnsembl.1
Natural variantiVAR_031924301D → Y.Corresponds to variant rs34324219dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05068 mRNA. Translation: AAA61058.1.
AK292990 mRNA. Translation: BAF85679.1.
AP002347 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73861.1.
CCDSiCCDS7978.1.
PIRiA34227.
RefSeqiNP_001053.2. NM_001062.3.
UniGeneiHs.2012.

Genome annotation databases

EnsembliENST00000257264; ENSP00000257264; ENSG00000134827.
GeneIDi6947.
KEGGihsa:6947.
UCSCiuc001noj.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05068 mRNA. Translation: AAA61058.1.
AK292990 mRNA. Translation: BAF85679.1.
AP002347 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73861.1.
CCDSiCCDS7978.1.
PIRiA34227.
RefSeqiNP_001053.2. NM_001062.3.
UniGeneiHs.2012.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CKVmodel-Y24-433[»]
4KKIX-ray2.35A1-433[»]
4KKJX-ray3.00A1-433[»]
ProteinModelPortaliP20061.
SMRiP20061.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112807. 3 interactors.
IntActiP20061. 1 interactor.
STRINGi9606.ENSP00000257264.

Chemistry databases

DrugBankiDB00115. Cyanocobalamin.
DB00200. Hydroxocobalamin.

PTM databases

iPTMnetiP20061.
PhosphoSitePlusiP20061.

Polymorphism and mutation databases

BioMutaiTCN1.
DMDMi146345530.

Proteomic databases

PaxDbiP20061.
PeptideAtlasiP20061.
PRIDEiP20061.

Protocols and materials databases

DNASUi6947.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000257264; ENSP00000257264; ENSG00000134827.
GeneIDi6947.
KEGGihsa:6947.
UCSCiuc001noj.3. human.

Organism-specific databases

CTDi6947.
DisGeNETi6947.
GeneCardsiTCN1.
H-InvDBHIX0009662.
HGNCiHGNC:11652. TCN1.
MIMi189905. gene.
neXtProtiNX_P20061.
OpenTargetsiENSG00000134827.
PharmGKBiPA36403.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IVBN. Eukaryota.
ENOG410XSU1. LUCA.
GeneTreeiENSGT00530000063370.
HOGENOMiHOG000013214.
HOVERGENiHBG094393.
InParanoidiP20061.
OMAiNNDRTYW.
OrthoDBiEOG091G095C.
PhylomeDBiP20061.
TreeFamiTF333092.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000134827-MONOMER.
ReactomeiR-HSA-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.
R-HSA-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiTCN1. human.
GeneWikiiHaptocorrin.
GenomeRNAii6947.
PROiP20061.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000134827.
CleanExiHS_TCN1.
GenevisibleiP20061. HS.

Family and domain databases

InterProiIPR002157. Cbl-bd_transpt_euk.
IPR027954. DUF4430.
[Graphical view]
PANTHERiPTHR10559. PTHR10559. 1 hit.
PfamiPF01122. Cobalamin_bind. 1 hit.
PF14478. DUF4430. 1 hit.
[Graphical view]
PROSITEiPS00468. COBALAMIN_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTCO1_HUMAN
AccessioniPrimary (citable) accession number: P20061
Secondary accession number(s): A8KAC5, Q8WV77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: May 1, 2007
Last modified: November 30, 2016
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.