Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcobalamin-1

Gene

TCN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds vitamin B12 with femtomolar affinity and protects it from the acidic environment of the stomach.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei240 – 2401Cobalamin
Binding sitei243 – 2431Cobalamin
Binding sitei289 – 2891Cobalamin
Binding sitei433 – 4331Cobalamin

GO - Molecular functioni

  1. cobalamin binding Source: GO_Central

GO - Biological processi

  1. cobalamin metabolic process Source: GO_Central
  2. cobalamin transport Source: ProtInc
  3. cobalt ion transport Source: UniProtKB-KW
  4. small molecule metabolic process Source: Reactome
  5. vitamin metabolic process Source: Reactome
  6. water-soluble vitamin metabolic process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Cobalt transport, Ion transport, Transport

Keywords - Ligandi

Cobalt

Enzyme and pathway databases

ReactomeiREACT_163862. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcobalamin-1
Short name:
TC-1
Alternative name(s):
Haptocorrin
Short name:
HC
Protein R
Transcobalamin I
Short name:
TC I
Short name:
TCI
Gene namesi
Name:TCN1
Synonyms:TC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:11652. TCN1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: Reactome
  2. extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36403.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Add
BLAST
Chaini24 – 433410Transcobalamin-1PRO_0000005561Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 2651 Publication
Disulfide bondi105 ↔ 3081 Publication
Disulfide bondi155 ↔ 1971 Publication
Glycosylationi160 – 1601N-linked (GlcNAc...)Sequence Analysis
Glycosylationi216 – 2161N-linked (GlcNAc...)3 Publications
Glycosylationi316 – 3161N-linked (GlcNAc...)1 Publication
Glycosylationi337 – 3371N-linked (GlcNAc...)1 Publication
Glycosylationi343 – 3431N-linked (GlcNAc...)1 Publication
Glycosylationi349 – 3491N-linked (GlcNAc...)1 Publication
Glycosylationi354 – 3541N-linked (GlcNAc...)1 Publication
Glycosylationi369 – 3691N-linked (GlcNAc...)2 Publications
Disulfide bondi388 ↔ 3931 Publication

Post-translational modificationi

Contains about 30% carbohydrates.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP20061.
PRIDEiP20061.

PTM databases

PhosphoSiteiP20061.

Expressioni

Tissue specificityi

Produced by the salivary glands of the oral cavity, in response to ingestion of food. Major constituent of secondary granules in neutrophils.1 Publication

Gene expression databases

BgeeiP20061.
CleanExiHS_TCN1.
GenevestigatoriP20061.

Organism-specific databases

HPAiHPA041631.

Interactioni

Protein-protein interaction databases

BioGridi112807. 2 interactions.
IntActiP20061. 1 interaction.
STRINGi9606.ENSP00000257264.

Structurei

Secondary structure

1
433
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi30 – 367Combined sources
Helixi37 – 459Combined sources
Helixi46 – 483Combined sources
Beta strandi50 – 523Combined sources
Helixi55 – 639Combined sources
Helixi69 – 8517Combined sources
Turni87 – 893Combined sources
Helixi92 – 10211Combined sources
Helixi108 – 1103Combined sources
Helixi111 – 1155Combined sources
Helixi118 – 13518Combined sources
Turni136 – 1383Combined sources
Helixi144 – 15613Combined sources
Helixi163 – 1697Combined sources
Helixi175 – 1773Combined sources
Helixi185 – 20319Combined sources
Helixi213 – 22917Combined sources
Beta strandi238 – 2403Combined sources
Turni241 – 2433Combined sources
Helixi244 – 2529Combined sources
Helixi255 – 2573Combined sources
Turni260 – 2623Combined sources
Helixi265 – 27713Combined sources
Turni278 – 2814Combined sources
Helixi284 – 29411Combined sources
Helixi299 – 3013Combined sources
Beta strandi333 – 35321Combined sources
Helixi358 – 36811Combined sources
Helixi370 – 3734Combined sources
Beta strandi375 – 3795Combined sources
Beta strandi381 – 3899Combined sources
Turni396 – 3994Combined sources
Beta strandi400 – 4067Combined sources
Turni415 – 4173Combined sources
Beta strandi426 – 4327Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CKVmodel-Y24-433[»]
4KKIX-ray2.35A1-433[»]
4KKJX-ray3.00A1-433[»]
SMRiP20061. Positions 24-433.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 310287Globular N-terminal alpha domainAdd
BLAST
Regioni142 – 1465Cobalamin binding
Regioni182 – 1865Cobalamin binding
Regioni311 – 33222Flexible linkerAdd
BLAST
Regioni333 – 433101Globular C-terminal beta domainAdd
BLAST
Regioni402 – 4043Cobalamin binding

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG79743.
GeneTreeiENSGT00530000063370.
HOGENOMiHOG000013214.
HOVERGENiHBG094393.
InParanoidiP20061.
OMAiENLEVRW.
OrthoDBiEOG771277.
PhylomeDBiP20061.
TreeFamiTF333092.

Family and domain databases

InterProiIPR002157. Cbl-bd_transpt_euk.
IPR027954. DUF4430.
[Graphical view]
PANTHERiPTHR10559. PTHR10559. 1 hit.
PfamiPF01122. Cobalamin_bind. 1 hit.
PF14478. DUF4430. 1 hit.
[Graphical view]
PROSITEiPS00468. COBALAMIN_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQSHQLPLV GLLLFSFIPS QLCEICEVSE ENYIRLKPLL NTMIQSNYNR
60 70 80 90 100
GTSAVNVVLS LKLVGIQIQT LMQKMIQQIK YNVKSRLSDV SSGELALIIL
110 120 130 140 150
ALGVCRNAEE NLIYDYHLID KLENKFQAEI ENMEAHNGTP LTNYYQLSLD
160 170 180 190 200
VLALCLFNGN YSTAEVVNHF TPENKNYYFG SQFSVDTGAM AVLALTCVKK
210 220 230 240 250
SLINGQIKAD EGSLKNISIY TKSLVEKILS EKKENGLIGN TFSTGEAMQA
260 270 280 290 300
LFVSSDYYNE NDWNCQQTLN TVLTEISQGA FSNPNAAAQV LPALMGKTFL
310 320 330 340 350
DINKDSSCVS ASGNFNISAD EPITVTPPDS QSYISVNYSV RINETYFTNV
360 370 380 390 400
TVLNGSVFLS VMEKAQKMND TIFGFTMEER SWGPYITCIQ GLCANNNDRT
410 420 430
YWELLSGGEP LSQGAGSYVV RNGENLEVRW SKY
Length:433
Mass (Da):48,207
Last modified:April 30, 2007 - v2
Checksum:iCF1052BE3DBA929A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti119 – 1191I → T in AAA61058 (PubMed:2777761).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti35 – 351R → H.
Corresponds to variant rs34528912 [ dbSNP | Ensembl ].
VAR_031923
Natural varianti301 – 3011D → Y.
Corresponds to variant rs34324219 [ dbSNP | Ensembl ].
VAR_031924

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05068 mRNA. Translation: AAA61058.1.
AK292990 mRNA. Translation: BAF85679.1.
AP002347 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73861.1.
CCDSiCCDS7978.1.
PIRiA34227.
RefSeqiNP_001053.2. NM_001062.3.
UniGeneiHs.2012.

Genome annotation databases

EnsembliENST00000257264; ENSP00000257264; ENSG00000134827.
GeneIDi6947.
KEGGihsa:6947.
UCSCiuc001noj.2. human.

Polymorphism databases

DMDMi146345530.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05068 mRNA. Translation: AAA61058.1.
AK292990 mRNA. Translation: BAF85679.1.
AP002347 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73861.1.
CCDSiCCDS7978.1.
PIRiA34227.
RefSeqiNP_001053.2. NM_001062.3.
UniGeneiHs.2012.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CKVmodel-Y24-433[»]
4KKIX-ray2.35A1-433[»]
4KKJX-ray3.00A1-433[»]
SMRiP20061. Positions 24-433.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112807. 2 interactions.
IntActiP20061. 1 interaction.
STRINGi9606.ENSP00000257264.

Chemistry

DrugBankiDB00115. Cyanocobalamin.
DB00200. Hydroxocobalamin.

PTM databases

PhosphoSiteiP20061.

Polymorphism databases

DMDMi146345530.

Proteomic databases

PaxDbiP20061.
PRIDEiP20061.

Protocols and materials databases

DNASUi6947.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000257264; ENSP00000257264; ENSG00000134827.
GeneIDi6947.
KEGGihsa:6947.
UCSCiuc001noj.2. human.

Organism-specific databases

CTDi6947.
GeneCardsiGC11M059620.
H-InvDBHIX0009662.
HGNCiHGNC:11652. TCN1.
HPAiHPA041631.
MIMi189905. gene.
neXtProtiNX_P20061.
PharmGKBiPA36403.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG79743.
GeneTreeiENSGT00530000063370.
HOGENOMiHOG000013214.
HOVERGENiHBG094393.
InParanoidiP20061.
OMAiENLEVRW.
OrthoDBiEOG771277.
PhylomeDBiP20061.
TreeFamiTF333092.

Enzyme and pathway databases

ReactomeiREACT_163862. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Miscellaneous databases

ChiTaRSiTCN1. human.
GeneWikiiHaptocorrin.
GenomeRNAii6947.
NextBioi27199.
PROiP20061.
SOURCEiSearch...

Gene expression databases

BgeeiP20061.
CleanExiHS_TCN1.
GenevestigatoriP20061.

Family and domain databases

InterProiIPR002157. Cbl-bd_transpt_euk.
IPR027954. DUF4430.
[Graphical view]
PANTHERiPTHR10559. PTHR10559. 1 hit.
PfamiPF01122. Cobalamin_bind. 1 hit.
PF14478. DUF4430. 1 hit.
[Graphical view]
PROSITEiPS00468. COBALAMIN_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of the cDNA encoding transcobalamin I, a neutrophil granule protein."
    Johnston J., Bollekens J., Allen R.H., Berliner N.
    J. Biol. Chem. 264:15754-15757(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Trachea.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
    Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
    J. Proteome Res. 4:2070-2080(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-216.
    Tissue: Plasma.
  6. "Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
    Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A.
    J. Proteome Res. 5:1493-1503(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-216 AND ASN-369.
    Tissue: Saliva.
  7. "Structural basis for universal corrinoid recognition by the cobalamin transport protein haptocorrin."
    Furger E., Frei D.C., Schibli R., Fischer E., Prota A.E.
    J. Biol. Chem. 288:25466-25476(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEX WITH CYANOCOBALAMIN, DISULFIDE BONDS, COBALAMIN-BINDING SITES, GLYCOSYLATION AT ASN-216; ASN-316; ASN-337; ASN-343; ASN-349; ASN-354 AND ASN-369.

Entry informationi

Entry nameiTCO1_HUMAN
AccessioniPrimary (citable) accession number: P20061
Secondary accession number(s): A8KAC5, Q8WV77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1991
Last sequence update: April 30, 2007
Last modified: March 31, 2015
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.