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P20061

- TCO1_HUMAN

UniProt

P20061 - TCO1_HUMAN

Protein

Transcobalamin-1

Gene

TCN1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (01 May 2007)
      Previous versions | rss
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    Functioni

    Binds vitamin B12 with femtomolar affinity and protects it from the acidic environment of the stomach.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei240 – 2401Cobalamin
    Binding sitei243 – 2431Cobalamin
    Binding sitei289 – 2891Cobalamin
    Binding sitei433 – 4331Cobalamin

    GO - Molecular functioni

    1. cobalamin binding Source: RefGenome

    GO - Biological processi

    1. cobalamin metabolic process Source: RefGenome
    2. cobalamin transport Source: ProtInc
    3. cobalt ion transport Source: UniProtKB-KW
    4. small molecule metabolic process Source: Reactome
    5. vitamin metabolic process Source: Reactome
    6. water-soluble vitamin metabolic process Source: Reactome

    Keywords - Biological processi

    Cobalt transport, Ion transport, Transport

    Keywords - Ligandi

    Cobalt

    Enzyme and pathway databases

    ReactomeiREACT_163862. Cobalamin (Cbl, vitamin B12) transport and metabolism.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcobalamin-1
    Short name:
    TC-1
    Alternative name(s):
    Haptocorrin
    Short name:
    HC
    Protein R
    Transcobalamin I
    Short name:
    TC I
    Short name:
    TCI
    Gene namesi
    Name:TCN1
    Synonyms:TC1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:11652. TCN1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: Reactome
    2. extracellular space Source: UniProt

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA36403.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Add
    BLAST
    Chaini24 – 433410Transcobalamin-1PRO_0000005561Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi26 ↔ 2651 Publication
    Disulfide bondi105 ↔ 3081 Publication
    Disulfide bondi155 ↔ 1971 Publication
    Glycosylationi160 – 1601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi216 – 2161N-linked (GlcNAc...)3 Publications
    Glycosylationi316 – 3161N-linked (GlcNAc...)1 Publication
    Glycosylationi337 – 3371N-linked (GlcNAc...)1 Publication
    Glycosylationi343 – 3431N-linked (GlcNAc...)1 Publication
    Glycosylationi349 – 3491N-linked (GlcNAc...)1 Publication
    Glycosylationi354 – 3541N-linked (GlcNAc...)1 Publication
    Glycosylationi369 – 3691N-linked (GlcNAc...)2 Publications
    Disulfide bondi388 ↔ 3931 Publication

    Post-translational modificationi

    Contains about 30% carbohydrates.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP20061.
    PRIDEiP20061.

    PTM databases

    PhosphoSiteiP20061.

    Expressioni

    Tissue specificityi

    Produced by the salivary glands of the oral cavity, in response to ingestion of food. Major constituent of secondary granules in neutrophils.1 Publication

    Gene expression databases

    BgeeiP20061.
    CleanExiHS_TCN1.
    GenevestigatoriP20061.

    Organism-specific databases

    HPAiHPA041631.

    Interactioni

    Protein-protein interaction databases

    BioGridi112807. 2 interactions.
    IntActiP20061. 1 interaction.
    STRINGi9606.ENSP00000257264.

    Structurei

    Secondary structure

    1
    433
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi30 – 367
    Helixi37 – 459
    Helixi46 – 483
    Beta strandi50 – 523
    Helixi55 – 639
    Helixi69 – 8517
    Turni87 – 893
    Helixi92 – 10211
    Helixi108 – 1103
    Helixi111 – 1155
    Helixi118 – 13518
    Turni136 – 1383
    Helixi144 – 15613
    Helixi163 – 1697
    Helixi175 – 1773
    Helixi185 – 20319
    Helixi213 – 22917
    Beta strandi238 – 2403
    Turni241 – 2433
    Helixi244 – 2529
    Helixi255 – 2573
    Turni260 – 2623
    Helixi265 – 27713
    Turni278 – 2814
    Helixi284 – 29411
    Helixi299 – 3013
    Beta strandi333 – 35321
    Helixi358 – 36811
    Helixi370 – 3734
    Beta strandi375 – 3795
    Beta strandi381 – 3899
    Turni396 – 3994
    Beta strandi400 – 4067
    Turni415 – 4173
    Beta strandi426 – 4327

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CKVmodel-Y24-433[»]
    4KKIX-ray2.35A1-433[»]
    4KKJX-ray3.00A1-433[»]
    ProteinModelPortaliP20061.
    SMRiP20061. Positions 24-433.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni24 – 310287Globular N-terminal alpha domainAdd
    BLAST
    Regioni142 – 1465Cobalamin binding
    Regioni182 – 1865Cobalamin binding
    Regioni311 – 33222Flexible linkerAdd
    BLAST
    Regioni333 – 433101Globular C-terminal beta domainAdd
    BLAST
    Regioni402 – 4043Cobalamin binding

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG79743.
    HOGENOMiHOG000013214.
    HOVERGENiHBG094393.
    InParanoidiP20061.
    OMAiENLEVRW.
    OrthoDBiEOG771277.
    PhylomeDBiP20061.
    TreeFamiTF333092.

    Family and domain databases

    InterProiIPR002157. Cbl-bd_transpt_euk.
    IPR027954. DUF4430.
    [Graphical view]
    PANTHERiPTHR10559. PTHR10559. 1 hit.
    PfamiPF01122. Cobalamin_bind. 1 hit.
    PF14478. DUF4430. 1 hit.
    [Graphical view]
    PROSITEiPS00468. COBALAMIN_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P20061-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRQSHQLPLV GLLLFSFIPS QLCEICEVSE ENYIRLKPLL NTMIQSNYNR    50
    GTSAVNVVLS LKLVGIQIQT LMQKMIQQIK YNVKSRLSDV SSGELALIIL 100
    ALGVCRNAEE NLIYDYHLID KLENKFQAEI ENMEAHNGTP LTNYYQLSLD 150
    VLALCLFNGN YSTAEVVNHF TPENKNYYFG SQFSVDTGAM AVLALTCVKK 200
    SLINGQIKAD EGSLKNISIY TKSLVEKILS EKKENGLIGN TFSTGEAMQA 250
    LFVSSDYYNE NDWNCQQTLN TVLTEISQGA FSNPNAAAQV LPALMGKTFL 300
    DINKDSSCVS ASGNFNISAD EPITVTPPDS QSYISVNYSV RINETYFTNV 350
    TVLNGSVFLS VMEKAQKMND TIFGFTMEER SWGPYITCIQ GLCANNNDRT 400
    YWELLSGGEP LSQGAGSYVV RNGENLEVRW SKY 433
    Length:433
    Mass (Da):48,207
    Last modified:May 1, 2007 - v2
    Checksum:iCF1052BE3DBA929A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti119 – 1191I → T in AAA61058. (PubMed:2777761)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti35 – 351R → H.
    Corresponds to variant rs34528912 [ dbSNP | Ensembl ].
    VAR_031923
    Natural varianti301 – 3011D → Y.
    Corresponds to variant rs34324219 [ dbSNP | Ensembl ].
    VAR_031924

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05068 mRNA. Translation: AAA61058.1.
    AK292990 mRNA. Translation: BAF85679.1.
    AP002347 Genomic DNA. No translation available.
    CH471076 Genomic DNA. Translation: EAW73861.1.
    CCDSiCCDS7978.1.
    PIRiA34227.
    RefSeqiNP_001053.2. NM_001062.3.
    UniGeneiHs.2012.

    Genome annotation databases

    EnsembliENST00000257264; ENSP00000257264; ENSG00000134827.
    GeneIDi6947.
    KEGGihsa:6947.
    UCSCiuc001noj.2. human.

    Polymorphism databases

    DMDMi146345530.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05068 mRNA. Translation: AAA61058.1 .
    AK292990 mRNA. Translation: BAF85679.1 .
    AP002347 Genomic DNA. No translation available.
    CH471076 Genomic DNA. Translation: EAW73861.1 .
    CCDSi CCDS7978.1.
    PIRi A34227.
    RefSeqi NP_001053.2. NM_001062.3.
    UniGenei Hs.2012.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CKV model - Y 24-433 [» ]
    4KKI X-ray 2.35 A 1-433 [» ]
    4KKJ X-ray 3.00 A 1-433 [» ]
    ProteinModelPortali P20061.
    SMRi P20061. Positions 24-433.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112807. 2 interactions.
    IntActi P20061. 1 interaction.
    STRINGi 9606.ENSP00000257264.

    Chemistry

    DrugBanki DB00115. Cyanocobalamin.
    DB00200. Hydroxocobalamin.

    PTM databases

    PhosphoSitei P20061.

    Polymorphism databases

    DMDMi 146345530.

    Proteomic databases

    PaxDbi P20061.
    PRIDEi P20061.

    Protocols and materials databases

    DNASUi 6947.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000257264 ; ENSP00000257264 ; ENSG00000134827 .
    GeneIDi 6947.
    KEGGi hsa:6947.
    UCSCi uc001noj.2. human.

    Organism-specific databases

    CTDi 6947.
    GeneCardsi GC11M059620.
    H-InvDB HIX0009662.
    HGNCi HGNC:11652. TCN1.
    HPAi HPA041631.
    MIMi 189905. gene.
    neXtProti NX_P20061.
    PharmGKBi PA36403.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG79743.
    HOGENOMi HOG000013214.
    HOVERGENi HBG094393.
    InParanoidi P20061.
    OMAi ENLEVRW.
    OrthoDBi EOG771277.
    PhylomeDBi P20061.
    TreeFami TF333092.

    Enzyme and pathway databases

    Reactomei REACT_163862. Cobalamin (Cbl, vitamin B12) transport and metabolism.

    Miscellaneous databases

    GeneWikii Haptocorrin.
    GenomeRNAii 6947.
    NextBioi 27199.
    PROi P20061.
    SOURCEi Search...

    Gene expression databases

    Bgeei P20061.
    CleanExi HS_TCN1.
    Genevestigatori P20061.

    Family and domain databases

    InterProi IPR002157. Cbl-bd_transpt_euk.
    IPR027954. DUF4430.
    [Graphical view ]
    PANTHERi PTHR10559. PTHR10559. 1 hit.
    Pfami PF01122. Cobalamin_bind. 1 hit.
    PF14478. DUF4430. 1 hit.
    [Graphical view ]
    PROSITEi PS00468. COBALAMIN_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the cDNA encoding transcobalamin I, a neutrophil granule protein."
      Johnston J., Bollekens J., Allen R.H., Berliner N.
      J. Biol. Chem. 264:15754-15757(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Trachea.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
      Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
      J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-216.
      Tissue: Plasma.
    6. "Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
      Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A.
      J. Proteome Res. 5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-216 AND ASN-369.
      Tissue: Saliva.
    7. "Structural basis for universal corrinoid recognition by the cobalamin transport protein haptocorrin."
      Furger E., Frei D.C., Schibli R., Fischer E., Prota A.E.
      J. Biol. Chem. 288:25466-25476(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEX WITH CYANOCOBALAMIN, DISULFIDE BONDS, COBALAMIN-BINDING SITES, GLYCOSYLATION AT ASN-216; ASN-316; ASN-337; ASN-343; ASN-349; ASN-354 AND ASN-369.

    Entry informationi

    Entry nameiTCO1_HUMAN
    AccessioniPrimary (citable) accession number: P20061
    Secondary accession number(s): A8KAC5, Q8WV77
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3