P20060 (HEXB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-hexosaminidase subunit beta
EC=3.2.1.52

Alternative name(s):
Beta-N-acetylhexosaminidase subunit beta
Short name=Hexosaminidase subunit B
N-acetyl-beta-glucosaminidase subunit beta

Gene names

Name:

Hexb

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the glycosyl hydrolase 20 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	astrocyte cell migration Inferred from mutant phenotype PubMed 15155903. Source: UniProtKB cellular calcium ion homeostasis Inferred from mutant phenotype PubMed 12756243. Source: MGI cellular protein metabolic process Inferred from mutant phenotype PubMed 12657883. Source: MGI ganglioside catabolic process Inferred from mutant phenotype PubMed 10021458PubMed 12657883PubMed 12756243PubMed 15748167PubMed 8566348PubMed 8789434PubMed 9223328PubMed 9645704. Source: MGI glycosaminoglycan metabolic process Inferred from genetic interaction PubMed 8896570. Source: MGI lipid storage Inferred from mutant phenotype PubMed 10021458PubMed 7550345PubMed 8789434PubMed 9223328. Source: MGI locomotory behavior Inferred from mutant phenotype PubMed 7550345. Source: MGI lysosome organization Inferred from mutant phenotype PubMed 8789434PubMed 9645704. Source: MGI male courtship behavior Inferred from mutant phenotype PubMed 12617783. Source: MGI myelination Inferred from genetic interaction PubMed 9184660. Source: MGI neuromuscular process controlling balance Inferred from mutant phenotype PubMed 10021458PubMed 7550345PubMed 9584189. Source: MGI oligosaccharide catabolic process Inferred from mutant phenotype PubMed 10021458PubMed 15748167. Source: MGI oogenesis Inferred from mutant phenotype PubMed 9223328. Source: MGI penetration of zona pellucida Inferred from mutant phenotype PubMed 8269854. Source: MGI phospholipid biosynthetic process Inferred from mutant phenotype PubMed 15198669. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 15155903. Source: UniProtKB regulation of cell shape Inferred from mutant phenotype PubMed 15155903. Source: UniProtKB sensory perception of sound Inferred from genetic interaction PubMed 8896570. Source: MGI skeletal system development Inferred from genetic interaction PubMed 8896570PubMed 9184660. Source: MGI
Cellular_component	acrosomal vesicle Inferred from direct assay PubMed 8269854. Source: MGI lysosome Inferred from direct assay PubMed 11854359. Source: MGI membrane Inferred from direct assay PubMed 11854359. Source: MGI
Molecular_function	beta-N-acetylhexosaminidase activity Inferred from direct assay PubMed 11854359PubMed 7203014PubMed 8269854PubMed 9122231PubMed 9417048. Source: MGI protein homodimerization activity Inferred from direct assay PubMed 8269854. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 536	505	Beta-hexosaminidase subunit beta		PRO_0000012006
Sites
	Active site	334	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	63	1	N-linked (GlcNAc...) Potential
	Glycosylation	169	1	N-linked (GlcNAc...) Potential
	Glycosylation	306	1	N-linked (GlcNAc...) Potential
	Disulfide bond	70 ↔ 116		By similarity
	Disulfide bond	288 ↔ 339		By similarity
	Disulfide bond	513 ↔ 530		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20060 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 579BBEEE9CB508BC	FASTA	536	61,116
	10 20 30 40 50 60 MPQSPRSAPG LLLLQALVSL VSLALVAPAR LQPALWPFPR SVQMFPRLLY ISAEDFSIDH 70 80 90 100 110 120 SPNSTAGPSC SLLQEAFRRY YNYVFGFYKR HHGPARFRAE PQLQKLLVSI TLESECESFP 130 140 150 160 170 180 SLSSDETYSL LVQEPVAVLK ANSVWGALRG LETFSQLVYQ DSFGTFTINE SSIADSPRFP 190 200 210 220 230 240 HRGILIDTSR HFLPVKTILK TLDAMAFNKF NVLHWHIVDD QSFPYQSTTF PELSNKGSYS 250 260 270 280 290 300 LSHVYTPNDV RMVLEYARLR GIRVIPEFDT PGHTQSWGKG QKNLLTPCYN QKTKTQVFGP 310 320 330 340 350 360 VDPTVNTTYA FFNTFFKEIS SVFPDQFIHL GGDEVEFQCW ASNPNIQGFM KRKGFGSDFR 370 380 390 400 410 420 RLESFYIKKI LEIISSLKKN SIVWQEVFDD KVELQPGTVV EVWKSEHYSY ELKQVTGSGF 430 440 450 460 470 480 PAILSAPWYL DLISYGQDWK NYYKVEPLNF EGSEKQKQLV IGGEACLWGE FVDATNLTPR 490 500 510 520 530 LWPRASAVGE RLWSPKTVTD LENAYKRLAV HRCRMVSRGI AAQPLYTGYC NYENKI

References

[1]	"Cloning and sequence analysis of a cDNA encoding the beta-subunit of mouse beta-hexosaminidase." Bapat B., Ethier M., Neote K., Mahuran D., Gravel R.A. FEBS Lett. 237:191-195(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structure and expression of the mouse beta-hexosaminidase genes, Hexa and Hexb." Yamanaka S., Johnson O.N., Norflus F., Boles D.J., Proia R.L. Genomics 21:588-596(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6 X CBA. Tissue: Liver.
[3]	"Characterization of the murine beta-hexosaminidase (HEXB) gene." Triggs-Raine B.L., Benoit G., Salo T.J., Trasler J.M., Gravel R.A. Biochim. Biophys. Acta 1227:79-86(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00964 mRNA. Translation: CAA68781.1. U07633 mRNA. Translation: AAA18776.1. U07049 , U07036, U07037, U07038, U07039, U07040, U07041, U07042, U07043, U07044, U07045, U07046, U07047, U07048 Genomic DNA. Translation: AAA74738.1. U07742 , U07722, U07723, U07724, U07725, U07726, U07727, U07728, U07737, U07738, U07739, U07740, U07741 Genomic DNA. Translation: AAB60667.1.
IPI	IPI00115530.
PIR	B54745.
RefSeq	NP_034552.1. NM_010422.2.
UniGene	Mm.27816.
3D structure databases
ProteinModelPortal	P20060.
SMR	P20060. Positions 33-532.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000022169.
Protein family/group databases
CAZy	GH20. Glycoside Hydrolase Family 20.
PTM databases
PhosphoSite	P20060.
Proteomic databases
PaxDb	P20060.
PRIDE	P20060.
Protocols and materials databases
DNASU	15212.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022169; ENSMUSP00000022169; ENSMUSG00000021665.
GeneID	15212.
KEGG	mmu:15212.
Organism-specific databases
CTD	3074.
MGI	MGI:96074. Hexb.
Phylogenomic databases
eggNOG	COG3525.
HOGENOM	HOG000157972.
HOVERGEN	HBG005961.
InParanoid	P20060.
KO	K12373.
OMA	PWYLDWI.
OrthoDB	EOG42Z4Q7.
Gene expression databases
ArrayExpress	P20060.
Bgee	P20060.
CleanEx	MM_HEXB.
Genevestigator	P20060.
GermOnline	ENSMUSG00000021665. Mus musculus.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR025705. Beta_hexosaminidase_sua/sub. IPR015883. Glyco_hydro_20_cat-core. IPR015882. Glyco_hydro_20b. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00728. Glyco_hydro_20. 1 hit. PF02838. Glyco_hydro_20b. 1 hit. [Graphical view]
PIRSF	PIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTS	PR00738. GLHYDRLASE20.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	HEXB. mouse.
NextBio	287781.
SOURCE	Search...

Entry information

Entry name

HEXB_MOUSE

Accession

Primary (citable) accession number: P20060

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program