Reviewed, UniProtKB/Swiss-Prot P20060 (HEXB_MOUSE)

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase subunit beta
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase subunit beta
    Beta-N-acetylhexosaminidase subunit beta
      Short name=Hexosaminidase subunit B

Gene names

Name:

Hexb

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the glycosyl hydrolase 20 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	cellular calcium ion homeostasis Inferred from genetic interaction. Source: MGI cellular protein metabolic process Inferred from mutant phenotype. Source: MGI ganglioside catabolic process Inferred from mutant phenotype. Source: MGI glycosaminoglycan metabolic process Inferred from genetic interaction. Source: MGI lipid storage Inferred from mutant phenotype. Source: MGI locomotory behavior Inferred from mutant phenotype. Source: MGI lysosome organization Inferred from mutant phenotype. Source: MGI male courtship behavior Inferred from mutant phenotype. Source: MGI myelination Inferred from genetic interaction. Source: MGI neuromuscular process controlling balance Inferred from mutant phenotype. Source: MGI oligosaccharide catabolic process Inferred from mutant phenotype. Source: MGI oogenesis Inferred from mutant phenotype. Source: MGI penetration of zona pellucida Inferred from mutant phenotype. Source: MGI phospholipid biosynthetic process Inferred from mutant phenotype. Source: MGI regulation of cellular metabolic process Inferred from mutant phenotype. Source: MGI sensory perception of sound Inferred from genetic interaction. Source: MGI skeletal system development Inferred from genetic interaction. Source: MGI
Cellular component	acrosomal vesicle Inferred from direct assay. Source: MGI lysosome Inferred from direct assay. Source: MGI membrane Inferred from direct assay. Source: MGI
Molecular function	beta-N-acetylhexosaminidase activity Inferred from direct assay. Source: MGI cation binding Inferred from electronic annotation. Source: InterPro protein homodimerization activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 536	505	Beta-hexosaminidase subunit beta		PRO_0000012006
Sites
	Active site	334	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	63	1	N-linked (GlcNAc...) Potential
	Glycosylation	169	1	N-linked (GlcNAc...) Potential
	Glycosylation	306	1	N-linked (GlcNAc...) Potential
	Disulfide bond	70 ↔ 116		By similarity
	Disulfide bond	288 ↔ 339		By similarity
	Disulfide bond	513 ↔ 530		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20060-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 579BBEEE9CB508BC	FASTA	536	61,116
	10 20 30 40 50 60 MPQSPRSAPG LLLLQALVSL VSLALVAPAR LQPALWPFPR SVQMFPRLLY ISAEDFSIDH 70 80 90 100 110 120 SPNSTAGPSC SLLQEAFRRY YNYVFGFYKR HHGPARFRAE PQLQKLLVSI TLESECESFP 130 140 150 160 170 180 SLSSDETYSL LVQEPVAVLK ANSVWGALRG LETFSQLVYQ DSFGTFTINE SSIADSPRFP 190 200 210 220 230 240 HRGILIDTSR HFLPVKTILK TLDAMAFNKF NVLHWHIVDD QSFPYQSTTF PELSNKGSYS 250 260 270 280 290 300 LSHVYTPNDV RMVLEYARLR GIRVIPEFDT PGHTQSWGKG QKNLLTPCYN QKTKTQVFGP 310 320 330 340 350 360 VDPTVNTTYA FFNTFFKEIS SVFPDQFIHL GGDEVEFQCW ASNPNIQGFM KRKGFGSDFR 370 380 390 400 410 420 RLESFYIKKI LEIISSLKKN SIVWQEVFDD KVELQPGTVV EVWKSEHYSY ELKQVTGSGF 430 440 450 460 470 480 PAILSAPWYL DLISYGQDWK NYYKVEPLNF EGSEKQKQLV IGGEACLWGE FVDATNLTPR 490 500 510 520 530 LWPRASAVGE RLWSPKTVTD LENAYKRLAV HRCRMVSRGI AAQPLYTGYC NYENKI

References

[1]	"Cloning and sequence analysis of a cDNA encoding the beta-subunit of mouse beta-hexosaminidase." Bapat B., Ethier M., Neote K., Mahuran D., Gravel R.A. FEBS Lett. 237:191-195(1988) [PubMed: 2971567] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structure and expression of the mouse beta-hexosaminidase genes, Hexa and Hexb." Yamanaka S., Johnson O.N., Norflus F., Boles D.J., Proia R.L. Genomics 21:588-596(1994) [PubMed: 7959736] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6 X CBA. Tissue: Liver.
[3]	"Characterization of the murine beta-hexosaminidase (HEXB) gene." Triggs-Raine B.L., Benoit G., Salo T.J., Trasler J.M., Gravel R.A. Biochim. Biophys. Acta 1227:79-86(1994) [PubMed: 7918686] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y00964 mRNA. Translation: CAA68781.1. U07633 mRNA. Translation: AAA18776.1. U07049 , U07036, U07037, U07038, U07039, U07040, U07041, U07042, U07043, U07044, U07045, U07046, U07047, U07048 Genomic DNA. Translation: AAA74738.1. U07742 , U07722, U07723, U07724, U07725, U07726, U07727, U07728, U07737, U07738, U07739, U07740, U07741 Genomic DNA. Translation: AAB60667.1.
IPI	IPI00115530.
PIR	B54745.
RefSeq	NP_034552.1.
UniGene	Mm.27816
3D structure databases
HSSP	HSSP built from PDB template 1NOW based on UniProtKB P07686.
SMR	P20060. Positions 34-531.
ModBase	Search...
Protein-protein interaction databases
STRING	P20060.
Protein family/group databases
CAZy	GH20. Glycoside Hydrolase Family 20.
Proteomic databases
PRIDE	P20060.
Genome annotation databases
Ensembl	ENSMUST00000022169; ENSMUSP00000022169; ENSMUSG00000021665; Mus musculus. [Genome view]
GeneID	15212.
KEGG	mmu:15212.
UCSC	uc007roc.1. mouse.
Organism-specific databases
CTD	15212.
MGI	MGI:96074. Hexb.
Phylogenomic databases
HOGENOM	P20060.
HOVERGEN	P20060.
OMA	PWYLDWI.
Enzyme and pathway databases
BRENDA	3.2.1.52. 244.
Gene expression databases
ArrayExpress	P20060.
Bgee	P20060.
CleanEx	MM_HEXB.
Genevestigator	P20060.
GermOnline	ENSMUSG00000021665. Mus musculus.
Family and domain databases
InterPro	IPR001540. Glyco_hydro_20. IPR015883. Glyco_hydro_20_cat-core. IPR013781. Glyco_hydro_sg_catalytic. IPR015882. HexNAc-like_b. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR22600. Glyco_hydro_20. 1 hit.
Pfam	PF00728. Glyco_hydro_20. 1 hit. PF02838. Glyco_hydro_20b. 1 hit. [Graphical view]
PRINTS	PR00738. GLHYDRLASE20.
ProtoNet	Search...
Other Resources
NextBio	287781.
SOURCE	Search...

Entry information

Entry name

HEXB_MOUSE

Accession

Primary (citable) accession number: P20060

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)