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P20060

- HEXB_MOUSE

UniProt

P20060 - HEXB_MOUSE

Protein

Beta-hexosaminidase subunit beta

Gene

Hexb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.

    Catalytic activityi

    Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei334 – 3341Proton donorBy similarity

    GO - Molecular functioni

    1. beta-N-acetylhexosaminidase activity Source: MGI
    2. protein homodimerization activity Source: MGI

    GO - Biological processi

    1. astrocyte cell migration Source: UniProtKB
    2. cellular calcium ion homeostasis Source: MGI
    3. cellular protein metabolic process Source: MGI
    4. ganglioside catabolic process Source: MGI
    5. glycosaminoglycan metabolic process Source: MGI
    6. glycosphingolipid metabolic process Source: MGI
    7. lipid storage Source: MGI
    8. locomotory behavior Source: MGI
    9. lysosome organization Source: MGI
    10. male courtship behavior Source: MGI
    11. myelination Source: MGI
    12. neuromuscular process Source: MGI
    13. neuromuscular process controlling balance Source: MGI
    14. oligosaccharide catabolic process Source: MGI
    15. oogenesis Source: MGI
    16. penetration of zona pellucida Source: MGI
    17. phospholipid biosynthetic process Source: MGI
    18. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    19. regulation of cell shape Source: UniProtKB
    20. regulation of cellular metabolic process Source: MGI
    21. sensory perception of sound Source: MGI
    22. sexual reproduction Source: MGI
    23. single fertilization Source: MGI
    24. skeletal system development Source: MGI

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    ReactomeiREACT_196554. Hyaluronan uptake and degradation.
    REACT_198960. Keratan sulfate degradation.
    REACT_198981. CS/DS degradation.
    REACT_199008. Glycosphingolipid metabolism.

    Protein family/group databases

    CAZyiGH20. Glycoside Hydrolase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-hexosaminidase subunit beta (EC:3.2.1.52)
    Alternative name(s):
    Beta-N-acetylhexosaminidase subunit beta
    Short name:
    Hexosaminidase subunit B
    N-acetyl-beta-glucosaminidase subunit beta
    Gene namesi
    Name:Hexb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:96074. Hexb.

    Subcellular locationi

    GO - Cellular componenti

    1. acrosomal vesicle Source: MGI
    2. lysosome Source: MGI
    3. membrane Source: MGI

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Sequence AnalysisAdd
    BLAST
    Chaini32 – 536505Beta-hexosaminidase subunit betaPRO_0000012006Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi70 ↔ 116By similarity
    Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi288 ↔ 339By similarity
    Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi513 ↔ 530By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP20060.
    PaxDbiP20060.
    PRIDEiP20060.

    PTM databases

    PhosphoSiteiP20060.

    Expressioni

    Gene expression databases

    ArrayExpressiP20060.
    BgeeiP20060.
    CleanExiMM_HEXB.
    GenevestigatoriP20060.

    Interactioni

    Protein-protein interaction databases

    IntActiP20060. 1 interaction.
    MINTiMINT-4097385.
    STRINGi10090.ENSMUSP00000022169.

    Structurei

    3D structure databases

    ProteinModelPortaliP20060.
    SMRiP20060. Positions 33-532.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 20 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3525.
    HOGENOMiHOG000157972.
    HOVERGENiHBG005961.
    InParanoidiP20060.
    KOiK12373.
    OMAiTVHRCRM.
    OrthoDBiEOG7KDFB6.
    PhylomeDBiP20060.
    TreeFamiTF313036.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.30.379.10. 1 hit.
    InterProiIPR025705. Beta_hexosaminidase_sua/sub.
    IPR029018. Chitobiase/Hex_dom_2-like.
    IPR015883. Glyco_hydro_20_cat-core.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR029019. HEX_eukaryotic_N.
    [Graphical view]
    PfamiPF00728. Glyco_hydro_20. 1 hit.
    PF14845. Glycohydro_20b2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
    PRINTSiPR00738. GLHYDRLASE20.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P20060-1 [UniParc]FASTAAdd to Basket

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    MPQSPRSAPG LLLLQALVSL VSLALVAPAR LQPALWPFPR SVQMFPRLLY    50
    ISAEDFSIDH SPNSTAGPSC SLLQEAFRRY YNYVFGFYKR HHGPARFRAE 100
    PQLQKLLVSI TLESECESFP SLSSDETYSL LVQEPVAVLK ANSVWGALRG 150
    LETFSQLVYQ DSFGTFTINE SSIADSPRFP HRGILIDTSR HFLPVKTILK 200
    TLDAMAFNKF NVLHWHIVDD QSFPYQSTTF PELSNKGSYS LSHVYTPNDV 250
    RMVLEYARLR GIRVIPEFDT PGHTQSWGKG QKNLLTPCYN QKTKTQVFGP 300
    VDPTVNTTYA FFNTFFKEIS SVFPDQFIHL GGDEVEFQCW ASNPNIQGFM 350
    KRKGFGSDFR RLESFYIKKI LEIISSLKKN SIVWQEVFDD KVELQPGTVV 400
    EVWKSEHYSY ELKQVTGSGF PAILSAPWYL DLISYGQDWK NYYKVEPLNF 450
    EGSEKQKQLV IGGEACLWGE FVDATNLTPR LWPRASAVGE RLWSPKTVTD 500
    LENAYKRLAV HRCRMVSRGI AAQPLYTGYC NYENKI 536
    Length:536
    Mass (Da):61,116
    Last modified:February 1, 1996 - v2
    Checksum:i579BBEEE9CB508BC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00964 mRNA. Translation: CAA68781.1.
    U07633 mRNA. Translation: AAA18776.1.
    U07049
    , U07036, U07037, U07038, U07039, U07040, U07041, U07042, U07043, U07044, U07045, U07046, U07047, U07048 Genomic DNA. Translation: AAA74738.1.
    U07742
    , U07722, U07723, U07724, U07725, U07726, U07727, U07728, U07737, U07738, U07739, U07740, U07741 Genomic DNA. Translation: AAB60667.1.
    CCDSiCCDS26709.1.
    PIRiB54745.
    RefSeqiNP_034552.1. NM_010422.2.
    UniGeneiMm.27816.

    Genome annotation databases

    EnsembliENSMUST00000022169; ENSMUSP00000022169; ENSMUSG00000021665.
    GeneIDi15212.
    KEGGimmu:15212.
    UCSCiuc007roc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00964 mRNA. Translation: CAA68781.1 .
    U07633 mRNA. Translation: AAA18776.1 .
    U07049
    , U07036 , U07037 , U07038 , U07039 , U07040 , U07041 , U07042 , U07043 , U07044 , U07045 , U07046 , U07047 , U07048 Genomic DNA. Translation: AAA74738.1 .
    U07742
    , U07722 , U07723 , U07724 , U07725 , U07726 , U07727 , U07728 , U07737 , U07738 , U07739 , U07740 , U07741 Genomic DNA. Translation: AAB60667.1 .
    CCDSi CCDS26709.1.
    PIRi B54745.
    RefSeqi NP_034552.1. NM_010422.2.
    UniGenei Mm.27816.

    3D structure databases

    ProteinModelPortali P20060.
    SMRi P20060. Positions 33-532.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P20060. 1 interaction.
    MINTi MINT-4097385.
    STRINGi 10090.ENSMUSP00000022169.

    Protein family/group databases

    CAZyi GH20. Glycoside Hydrolase Family 20.

    PTM databases

    PhosphoSitei P20060.

    Proteomic databases

    MaxQBi P20060.
    PaxDbi P20060.
    PRIDEi P20060.

    Protocols and materials databases

    DNASUi 15212.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022169 ; ENSMUSP00000022169 ; ENSMUSG00000021665 .
    GeneIDi 15212.
    KEGGi mmu:15212.
    UCSCi uc007roc.2. mouse.

    Organism-specific databases

    CTDi 3074.
    MGIi MGI:96074. Hexb.

    Phylogenomic databases

    eggNOGi COG3525.
    HOGENOMi HOG000157972.
    HOVERGENi HBG005961.
    InParanoidi P20060.
    KOi K12373.
    OMAi TVHRCRM.
    OrthoDBi EOG7KDFB6.
    PhylomeDBi P20060.
    TreeFami TF313036.

    Enzyme and pathway databases

    Reactomei REACT_196554. Hyaluronan uptake and degradation.
    REACT_198960. Keratan sulfate degradation.
    REACT_198981. CS/DS degradation.
    REACT_199008. Glycosphingolipid metabolism.

    Miscellaneous databases

    ChiTaRSi HEXB. mouse.
    NextBioi 287781.
    PROi P20060.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P20060.
    Bgeei P20060.
    CleanExi MM_HEXB.
    Genevestigatori P20060.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    3.30.379.10. 1 hit.
    InterProi IPR025705. Beta_hexosaminidase_sua/sub.
    IPR029018. Chitobiase/Hex_dom_2-like.
    IPR015883. Glyco_hydro_20_cat-core.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR029019. HEX_eukaryotic_N.
    [Graphical view ]
    Pfami PF00728. Glyco_hydro_20. 1 hit.
    PF14845. Glycohydro_20b2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
    PRINTSi PR00738. GLHYDRLASE20.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of a cDNA encoding the beta-subunit of mouse beta-hexosaminidase."
      Bapat B., Ethier M., Neote K., Mahuran D., Gravel R.A.
      FEBS Lett. 237:191-195(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structure and expression of the mouse beta-hexosaminidase genes, Hexa and Hexb."
      Yamanaka S., Johnson O.N., Norflus F., Boles D.J., Proia R.L.
      Genomics 21:588-596(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: C57BL/6 X CBA.
      Tissue: Liver.
    3. "Characterization of the murine beta-hexosaminidase (HEXB) gene."
      Triggs-Raine B.L., Benoit G., Salo T.J., Trasler J.M., Gravel R.A.
      Biochim. Biophys. Acta 1227:79-86(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.

    Entry informationi

    Entry nameiHEXB_MOUSE
    AccessioniPrimary (citable) accession number: P20060
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3