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P20060

- HEXB_MOUSE

UniProt

P20060 - HEXB_MOUSE

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Protein

Beta-hexosaminidase subunit beta

Gene

Hexb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei334 – 3341Proton donorBy similarity

GO - Molecular functioni

  1. beta-N-acetylhexosaminidase activity Source: MGI
  2. protein homodimerization activity Source: MGI

GO - Biological processi

  1. astrocyte cell migration Source: UniProtKB
  2. cellular calcium ion homeostasis Source: MGI
  3. cellular protein metabolic process Source: MGI
  4. ganglioside catabolic process Source: MGI
  5. glycosaminoglycan metabolic process Source: MGI
  6. glycosphingolipid metabolic process Source: MGI
  7. lipid storage Source: MGI
  8. locomotory behavior Source: MGI
  9. lysosome organization Source: MGI
  10. male courtship behavior Source: MGI
  11. myelination Source: MGI
  12. neuromuscular process Source: MGI
  13. neuromuscular process controlling balance Source: MGI
  14. oligosaccharide catabolic process Source: MGI
  15. oogenesis Source: MGI
  16. penetration of zona pellucida Source: MGI
  17. phospholipid biosynthetic process Source: MGI
  18. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  19. regulation of cell shape Source: UniProtKB
  20. regulation of cellular metabolic process Source: MGI
  21. sensory perception of sound Source: MGI
  22. sexual reproduction Source: MGI
  23. single fertilization Source: MGI
  24. skeletal system development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiREACT_196554. Hyaluronan uptake and degradation.
REACT_198960. Keratan sulfate degradation.
REACT_198981. CS/DS degradation.
REACT_199008. Glycosphingolipid metabolism.

Protein family/group databases

CAZyiGH20. Glycoside Hydrolase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-hexosaminidase subunit beta (EC:3.2.1.52)
Alternative name(s):
Beta-N-acetylhexosaminidase subunit beta
Short name:
Hexosaminidase subunit B
N-acetyl-beta-glucosaminidase subunit beta
Gene namesi
Name:Hexb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:96074. Hexb.

Subcellular locationi

GO - Cellular componenti

  1. acrosomal vesicle Source: MGI
  2. extracellular vesicular exosome Source: Ensembl
  3. lysosome Source: MGI
  4. membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Chaini32 – 536505Beta-hexosaminidase subunit betaPRO_0000012006Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi70 ↔ 116By similarity
Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi288 ↔ 339By similarity
Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi513 ↔ 530By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP20060.
PaxDbiP20060.
PRIDEiP20060.

PTM databases

PhosphoSiteiP20060.

Expressioni

Gene expression databases

BgeeiP20060.
CleanExiMM_HEXB.
ExpressionAtlasiP20060. baseline and differential.
GenevestigatoriP20060.

Interactioni

Protein-protein interaction databases

IntActiP20060. 1 interaction.
MINTiMINT-4097385.
STRINGi10090.ENSMUSP00000022169.

Structurei

3D structure databases

ProteinModelPortaliP20060.
SMRiP20060. Positions 33-532.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 20 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3525.
HOGENOMiHOG000157972.
HOVERGENiHBG005961.
InParanoidiP20060.
KOiK12373.
OMAiTVHRCRM.
OrthoDBiEOG7KDFB6.
PhylomeDBiP20060.
TreeFamiTF313036.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProiIPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR029019. HEX_eukaryotic_N.
[Graphical view]
PfamiPF00728. Glyco_hydro_20. 1 hit.
PF14845. Glycohydro_20b2. 1 hit.
[Graphical view]
PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSiPR00738. GLHYDRLASE20.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20060-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPQSPRSAPG LLLLQALVSL VSLALVAPAR LQPALWPFPR SVQMFPRLLY
60 70 80 90 100
ISAEDFSIDH SPNSTAGPSC SLLQEAFRRY YNYVFGFYKR HHGPARFRAE
110 120 130 140 150
PQLQKLLVSI TLESECESFP SLSSDETYSL LVQEPVAVLK ANSVWGALRG
160 170 180 190 200
LETFSQLVYQ DSFGTFTINE SSIADSPRFP HRGILIDTSR HFLPVKTILK
210 220 230 240 250
TLDAMAFNKF NVLHWHIVDD QSFPYQSTTF PELSNKGSYS LSHVYTPNDV
260 270 280 290 300
RMVLEYARLR GIRVIPEFDT PGHTQSWGKG QKNLLTPCYN QKTKTQVFGP
310 320 330 340 350
VDPTVNTTYA FFNTFFKEIS SVFPDQFIHL GGDEVEFQCW ASNPNIQGFM
360 370 380 390 400
KRKGFGSDFR RLESFYIKKI LEIISSLKKN SIVWQEVFDD KVELQPGTVV
410 420 430 440 450
EVWKSEHYSY ELKQVTGSGF PAILSAPWYL DLISYGQDWK NYYKVEPLNF
460 470 480 490 500
EGSEKQKQLV IGGEACLWGE FVDATNLTPR LWPRASAVGE RLWSPKTVTD
510 520 530
LENAYKRLAV HRCRMVSRGI AAQPLYTGYC NYENKI
Length:536
Mass (Da):61,116
Last modified:February 1, 1996 - v2
Checksum:i579BBEEE9CB508BC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00964 mRNA. Translation: CAA68781.1.
U07633 mRNA. Translation: AAA18776.1.
U07049
, U07036, U07037, U07038, U07039, U07040, U07041, U07042, U07043, U07044, U07045, U07046, U07047, U07048 Genomic DNA. Translation: AAA74738.1.
U07742
, U07722, U07723, U07724, U07725, U07726, U07727, U07728, U07737, U07738, U07739, U07740, U07741 Genomic DNA. Translation: AAB60667.1.
CCDSiCCDS26709.1.
PIRiB54745.
RefSeqiNP_034552.1. NM_010422.2.
UniGeneiMm.27816.

Genome annotation databases

EnsembliENSMUST00000022169; ENSMUSP00000022169; ENSMUSG00000021665.
GeneIDi15212.
KEGGimmu:15212.
UCSCiuc007roc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00964 mRNA. Translation: CAA68781.1 .
U07633 mRNA. Translation: AAA18776.1 .
U07049
, U07036 , U07037 , U07038 , U07039 , U07040 , U07041 , U07042 , U07043 , U07044 , U07045 , U07046 , U07047 , U07048 Genomic DNA. Translation: AAA74738.1 .
U07742
, U07722 , U07723 , U07724 , U07725 , U07726 , U07727 , U07728 , U07737 , U07738 , U07739 , U07740 , U07741 Genomic DNA. Translation: AAB60667.1 .
CCDSi CCDS26709.1.
PIRi B54745.
RefSeqi NP_034552.1. NM_010422.2.
UniGenei Mm.27816.

3D structure databases

ProteinModelPortali P20060.
SMRi P20060. Positions 33-532.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P20060. 1 interaction.
MINTi MINT-4097385.
STRINGi 10090.ENSMUSP00000022169.

Protein family/group databases

CAZyi GH20. Glycoside Hydrolase Family 20.

PTM databases

PhosphoSitei P20060.

Proteomic databases

MaxQBi P20060.
PaxDbi P20060.
PRIDEi P20060.

Protocols and materials databases

DNASUi 15212.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022169 ; ENSMUSP00000022169 ; ENSMUSG00000021665 .
GeneIDi 15212.
KEGGi mmu:15212.
UCSCi uc007roc.2. mouse.

Organism-specific databases

CTDi 3074.
MGIi MGI:96074. Hexb.

Phylogenomic databases

eggNOGi COG3525.
HOGENOMi HOG000157972.
HOVERGENi HBG005961.
InParanoidi P20060.
KOi K12373.
OMAi TVHRCRM.
OrthoDBi EOG7KDFB6.
PhylomeDBi P20060.
TreeFami TF313036.

Enzyme and pathway databases

Reactomei REACT_196554. Hyaluronan uptake and degradation.
REACT_198960. Keratan sulfate degradation.
REACT_198981. CS/DS degradation.
REACT_199008. Glycosphingolipid metabolism.

Miscellaneous databases

ChiTaRSi HEXB. mouse.
NextBioi 287781.
PROi P20060.
SOURCEi Search...

Gene expression databases

Bgeei P20060.
CleanExi MM_HEXB.
ExpressionAtlasi P20060. baseline and differential.
Genevestigatori P20060.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProi IPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR029019. HEX_eukaryotic_N.
[Graphical view ]
Pfami PF00728. Glyco_hydro_20. 1 hit.
PF14845. Glycohydro_20b2. 1 hit.
[Graphical view ]
PIRSFi PIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSi PR00738. GLHYDRLASE20.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequence analysis of a cDNA encoding the beta-subunit of mouse beta-hexosaminidase."
    Bapat B., Ethier M., Neote K., Mahuran D., Gravel R.A.
    FEBS Lett. 237:191-195(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structure and expression of the mouse beta-hexosaminidase genes, Hexa and Hexb."
    Yamanaka S., Johnson O.N., Norflus F., Boles D.J., Proia R.L.
    Genomics 21:588-596(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C57BL/6 X CBA.
    Tissue: Liver.
  3. "Characterization of the murine beta-hexosaminidase (HEXB) gene."
    Triggs-Raine B.L., Benoit G., Salo T.J., Trasler J.M., Gravel R.A.
    Biochim. Biophys. Acta 1227:79-86(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.

Entry informationi

Entry nameiHEXB_MOUSE
AccessioniPrimary (citable) accession number: P20060
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3