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P20060 (HEXB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-hexosaminidase subunit beta

EC=3.2.1.52
Alternative name(s):
Beta-N-acetylhexosaminidase subunit beta
Short name=Hexosaminidase subunit B
N-acetyl-beta-glucosaminidase subunit beta
Gene names
Name:Hexb
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length536 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

Subcellular location

Lysosome.

Sequence similarities

Belongs to the glycosyl hydrolase 20 family.

Ontologies

Keywords
   Cellular componentLysosome
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processastrocyte cell migration

Inferred from mutant phenotype PubMed 15155903. Source: UniProtKB

cellular calcium ion homeostasis

Inferred from mutant phenotype PubMed 12756243. Source: MGI

cellular protein metabolic process

Inferred from mutant phenotype PubMed 12657883. Source: MGI

ganglioside catabolic process

Inferred from mutant phenotype PubMed 10021458PubMed 12657883PubMed 12756243PubMed 15748167PubMed 8566348PubMed 8789434PubMed 9223328PubMed 9645704. Source: MGI

glycosaminoglycan metabolic process

Inferred from genetic interaction PubMed 8896570. Source: MGI

glycosphingolipid metabolic process

Inferred from mutant phenotype PubMed 8789434. Source: MGI

lipid storage

Inferred from mutant phenotype PubMed 10021458PubMed 7550345PubMed 8789434PubMed 9223328. Source: MGI

locomotory behavior

Inferred from mutant phenotype PubMed 7550345. Source: MGI

lysosome organization

Inferred from mutant phenotype PubMed 8789434PubMed 9645704. Source: MGI

male courtship behavior

Inferred from mutant phenotype PubMed 12617783. Source: MGI

myelination

Inferred from genetic interaction PubMed 9184660. Source: MGI

neuromuscular process

Inferred from mutant phenotype PubMed 10021458PubMed 7550345PubMed 9584189. Source: MGI

neuromuscular process controlling balance

Inferred from mutant phenotype PubMed 10021458PubMed 7550345PubMed 9584189. Source: MGI

oligosaccharide catabolic process

Inferred from mutant phenotype PubMed 10021458PubMed 15748167. Source: MGI

oogenesis

Inferred from mutant phenotype PubMed 9223328. Source: MGI

penetration of zona pellucida

Inferred from mutant phenotype PubMed 8269854. Source: MGI

phospholipid biosynthetic process

Inferred from mutant phenotype PubMed 15198669. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 15155903. Source: UniProtKB

regulation of cell shape

Inferred from mutant phenotype PubMed 15155903. Source: UniProtKB

regulation of cellular metabolic process

Inferred from mutant phenotype PubMed 15198669. Source: MGI

sensory perception of sound

Inferred from genetic interaction PubMed 8896570. Source: MGI

sexual reproduction

Inferred from mutant phenotype PubMed 9645704. Source: MGI

single fertilization

Inferred from mutant phenotype PubMed 12756243. Source: MGI

skeletal system development

Inferred from genetic interaction PubMed 8896570PubMed 9184660. Source: MGI

   Cellular_componentacrosomal vesicle

Inferred from direct assay PubMed 8269854. Source: MGI

lysosome

Inferred from direct assay PubMed 11854359. Source: MGI

membrane

Inferred from direct assay PubMed 11854359. Source: MGI

   Molecular_functionbeta-N-acetylhexosaminidase activity

Inferred from direct assay PubMed 11854359PubMed 7203014PubMed 8269854PubMed 9122231PubMed 9417048. Source: MGI

protein homodimerization activity

Inferred from direct assay PubMed 8269854. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 536505Beta-hexosaminidase subunit beta
PRO_0000012006

Sites

Active site3341Proton donor By similarity

Amino acid modifications

Glycosylation631N-linked (GlcNAc...) Potential
Glycosylation1691N-linked (GlcNAc...) Potential
Glycosylation3061N-linked (GlcNAc...) Potential
Disulfide bond70 ↔ 116 By similarity
Disulfide bond288 ↔ 339 By similarity
Disulfide bond513 ↔ 530 By similarity

Sequences

Sequence LengthMass (Da)Tools
P20060 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 579BBEEE9CB508BC

FASTA53661,116
        10         20         30         40         50         60 
MPQSPRSAPG LLLLQALVSL VSLALVAPAR LQPALWPFPR SVQMFPRLLY ISAEDFSIDH 

        70         80         90        100        110        120 
SPNSTAGPSC SLLQEAFRRY YNYVFGFYKR HHGPARFRAE PQLQKLLVSI TLESECESFP 

       130        140        150        160        170        180 
SLSSDETYSL LVQEPVAVLK ANSVWGALRG LETFSQLVYQ DSFGTFTINE SSIADSPRFP 

       190        200        210        220        230        240 
HRGILIDTSR HFLPVKTILK TLDAMAFNKF NVLHWHIVDD QSFPYQSTTF PELSNKGSYS 

       250        260        270        280        290        300 
LSHVYTPNDV RMVLEYARLR GIRVIPEFDT PGHTQSWGKG QKNLLTPCYN QKTKTQVFGP 

       310        320        330        340        350        360 
VDPTVNTTYA FFNTFFKEIS SVFPDQFIHL GGDEVEFQCW ASNPNIQGFM KRKGFGSDFR 

       370        380        390        400        410        420 
RLESFYIKKI LEIISSLKKN SIVWQEVFDD KVELQPGTVV EVWKSEHYSY ELKQVTGSGF 

       430        440        450        460        470        480 
PAILSAPWYL DLISYGQDWK NYYKVEPLNF EGSEKQKQLV IGGEACLWGE FVDATNLTPR 

       490        500        510        520        530 
LWPRASAVGE RLWSPKTVTD LENAYKRLAV HRCRMVSRGI AAQPLYTGYC NYENKI 

« Hide

References

[1]"Cloning and sequence analysis of a cDNA encoding the beta-subunit of mouse beta-hexosaminidase."
Bapat B., Ethier M., Neote K., Mahuran D., Gravel R.A.
FEBS Lett. 237:191-195(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structure and expression of the mouse beta-hexosaminidase genes, Hexa and Hexb."
Yamanaka S., Johnson O.N., Norflus F., Boles D.J., Proia R.L.
Genomics 21:588-596(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C57BL/6 X CBA.
Tissue: Liver.
[3]"Characterization of the murine beta-hexosaminidase (HEXB) gene."
Triggs-Raine B.L., Benoit G., Salo T.J., Trasler J.M., Gravel R.A.
Biochim. Biophys. Acta 1227:79-86(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00964 mRNA. Translation: CAA68781.1.
U07633 mRNA. Translation: AAA18776.1.
U07049 expand/collapse EMBL AC list , U07036, U07037, U07038, U07039, U07040, U07041, U07042, U07043, U07044, U07045, U07046, U07047, U07048 Genomic DNA. Translation: AAA74738.1.
U07742 expand/collapse EMBL AC list , U07722, U07723, U07724, U07725, U07726, U07727, U07728, U07737, U07738, U07739, U07740, U07741 Genomic DNA. Translation: AAB60667.1.
CCDSCCDS26709.1.
PIRB54745.
RefSeqNP_034552.1. NM_010422.2.
UniGeneMm.27816.

3D structure databases

ProteinModelPortalP20060.
SMRP20060. Positions 33-532.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP20060. 1 interaction.
MINTMINT-4097385.
STRING10090.ENSMUSP00000022169.

Protein family/group databases

CAZyGH20. Glycoside Hydrolase Family 20.

PTM databases

PhosphoSiteP20060.

Proteomic databases

MaxQBP20060.
PaxDbP20060.
PRIDEP20060.

Protocols and materials databases

DNASU15212.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022169; ENSMUSP00000022169; ENSMUSG00000021665.
GeneID15212.
KEGGmmu:15212.
UCSCuc007roc.2. mouse.

Organism-specific databases

CTD3074.
MGIMGI:96074. Hexb.

Phylogenomic databases

eggNOGCOG3525.
HOGENOMHOG000157972.
HOVERGENHBG005961.
InParanoidP20060.
KOK12373.
OMATVHRCRM.
OrthoDBEOG7KDFB6.
PhylomeDBP20060.
TreeFamTF313036.

Gene expression databases

ArrayExpressP20060.
BgeeP20060.
CleanExMM_HEXB.
GenevestigatorP20060.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProIPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR029019. HEX_eukaryotic_N.
[Graphical view]
PfamPF00728. Glyco_hydro_20. 1 hit.
PF14845. Glycohydro_20b2. 1 hit.
[Graphical view]
PIRSFPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSPR00738. GLHYDRLASE20.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
ProtoNetSearch...

Other

ChiTaRSHEXB. mouse.
NextBio287781.
PROP20060.
SOURCESearch...

Entry information

Entry nameHEXB_MOUSE
AccessionPrimary (citable) accession number: P20060
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries