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Protein

Hemopexin

Gene

Hpx

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi79 – 791Iron (heme 1 axial ligand)By similarity
Metal bindingi149 – 1491Iron (heme 1 axial ligand)By similarity
Metal bindingi235 – 2351Iron (heme 2 axial ligand)By similarity
Metal bindingi291 – 2911Iron (heme 2 axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-2168880. Scavenging of heme from plasma.

Names & Taxonomyi

Protein namesi
Recommended name:
Hemopexin
Gene namesi
Name:Hpx
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi62040. Hpx.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2176811.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 460437HemopexinPRO_0000021410Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...)Sequence analysis
Disulfide bondi50 ↔ 230By similarity
Glycosylationi64 – 641N-linked (GlcNAc...)Sequence analysis
Disulfide bondi148 ↔ 153By similarity
Glycosylationi186 – 1861N-linked (GlcNAc...)Sequence analysis
Disulfide bondi187 ↔ 199By similarity
Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence analysis
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence analysis
Disulfide bondi255 ↔ 458By similarity
Disulfide bondi364 ↔ 406By similarity
Disulfide bondi416 ↔ 433By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP20059.
PRIDEiP20059.

PTM databases

iPTMnetiP20059.
PhosphoSiteiP20059.

Expressioni

Tissue specificityi

Expressed by the liver and secreted in plasma.

Gene expression databases

BgeeiENSRNOG00000018257.
GenevisibleiP20059. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024710.

Structurei

3D structure databases

ProteinModelPortaliP20059.
SMRiP20059. Positions 47-460.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati53 – 9341Hemopexin 1Add
BLAST
Repeati94 – 13845Hemopexin 2Add
BLAST
Repeati139 – 18345Hemopexin 3Add
BLAST
Repeati184 – 23047Hemopexin 4Add
BLAST
Repeati257 – 30246Hemopexin 5Add
BLAST
Repeati303 – 35048Hemopexin 6Add
BLAST
Repeati355 – 39440Hemopexin 7Add
BLAST
Repeati398 – 44851Hemopexin 8Add
BLAST

Sequence similaritiesi

Belongs to the hemopexin family.Curated
Contains 8 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00390000009178.
HOGENOMiHOG000112887.
HOVERGENiHBG005956.
InParanoidiP20059.
KOiK18977.
OMAiAVECHRG.
OrthoDBiEOG091G052J.
PhylomeDBiP20059.
TreeFamiTF331201.

Family and domain databases

Gene3Di2.110.10.10. 2 hits.
InterProiIPR016358. Hemopexin.
IPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
[Graphical view]
PfamiPF00045. Hemopexin. 4 hits.
[Graphical view]
PIRSFiPIRSF002551. Hemopexin_chordata. 1 hit.
SMARTiSM00120. HX. 5 hits.
[Graphical view]
SUPFAMiSSF50923. SSF50923. 2 hits.
PROSITEiPS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 8 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20059-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARTVVALNI LVLLGLCWSL AVANPLPAAH ETVAKGENGT KPDSDVIEHC
60 70 80 90 100
SDAWSFDATT MDHNGTMLFF KGEFVWRGHS GIRELISERW KNPVTSVDAA
110 120 130 140 150
FRGPDSVFLI KEDKVWVYPP EKKENGYPKL FQEEFPGIPY PPDAAVECHR
160 170 180 190 200
GECQSEGVLF FQGNRKWFWD FATRTQKERS WPAVGNCTAA LRWLERYYCF
210 220 230 240 250
QGNKFLRFNP VTGEVPPRYP LDARDYFISC PGRGHGKLRN GTAHGNSTHP
260 270 280 290 300
MHSRCNADPG LSALLSDHRG ATYAFSGSHY WRLDSSRDGW HSWPIAHHWP
310 320 330 340 350
QGPSAVDAAF SWDEKVYLIQ GTQVYVFLTK GGNNLVSGYP KRLEKELGSP
360 370 380 390 400
PGISLDTIDA AFSCPGSSKL YVTSGRRLWW LDLKSGAQAT WAELSWPHEK
410 420 430 440 450
VDGALCLEKS LGPYSCSSNG PNLFFIHGPN LYCYSSIDKL NAAKSLPQPQ
460
KVNSILGCSQ
Length:460
Mass (Da):51,351
Last modified:January 9, 2007 - v3
Checksum:i508963FEE0E31D92
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 381N → C AA sequence (PubMed:3421961).Curated
Sequence conflicti49 – 502HC → KW AA sequence (PubMed:3421961).Curated
Sequence conflicti135 – 1351F → S in AAA41337 (PubMed:1988069).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62642 Genomic DNA. Translation: AAA41337.1.
X60006 Genomic DNA. Translation: CAA42621.1.
BC091137 mRNA. Translation: AAH91137.1.
PIRiA43079. OQRT.
RefSeqiNP_445770.1. NM_053318.1.
UniGeneiRn.2380.

Genome annotation databases

EnsembliENSRNOT00000024710; ENSRNOP00000024710; ENSRNOG00000018257.
GeneIDi58917.
KEGGirno:58917.
UCSCiRGD:62040. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62642 Genomic DNA. Translation: AAA41337.1.
X60006 Genomic DNA. Translation: CAA42621.1.
BC091137 mRNA. Translation: AAH91137.1.
PIRiA43079. OQRT.
RefSeqiNP_445770.1. NM_053318.1.
UniGeneiRn.2380.

3D structure databases

ProteinModelPortaliP20059.
SMRiP20059. Positions 47-460.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024710.

Chemistry

ChEMBLiCHEMBL2176811.

PTM databases

iPTMnetiP20059.
PhosphoSiteiP20059.

Proteomic databases

PaxDbiP20059.
PRIDEiP20059.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024710; ENSRNOP00000024710; ENSRNOG00000018257.
GeneIDi58917.
KEGGirno:58917.
UCSCiRGD:62040. rat.

Organism-specific databases

CTDi3263.
RGDi62040. Hpx.

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00390000009178.
HOGENOMiHOG000112887.
HOVERGENiHBG005956.
InParanoidiP20059.
KOiK18977.
OMAiAVECHRG.
OrthoDBiEOG091G052J.
PhylomeDBiP20059.
TreeFamiTF331201.

Enzyme and pathway databases

ReactomeiR-RNO-2168880. Scavenging of heme from plasma.

Miscellaneous databases

PROiP20059.

Gene expression databases

BgeeiENSRNOG00000018257.
GenevisibleiP20059. RN.

Family and domain databases

Gene3Di2.110.10.10. 2 hits.
InterProiIPR016358. Hemopexin.
IPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
[Graphical view]
PfamiPF00045. Hemopexin. 4 hits.
[Graphical view]
PIRSFiPIRSF002551. Hemopexin_chordata. 1 hit.
SMARTiSM00120. HX. 5 hits.
[Graphical view]
SUPFAMiSSF50923. SSF50923. 2 hits.
PROSITEiPS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 8 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEMO_RAT
AccessioniPrimary (citable) accession number: P20059
Secondary accession number(s): Q5BKB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The isolated N-terminal domain binds one heme. The full-length protein also binds one heme, but at a different site. The physiological significance of this is not clear (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.