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P20053

- PRP4_YEAST

UniProt

P20053 - PRP4_YEAST

Protein

U4/U6 small nuclear ribonucleoprotein PRP4

Gene

PRP4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Involved in RNA splicing. Is required for the association of U4/U6 snRNP with U5 snRNP in an early step of spliceosome assembly.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: SGD
    2. snoRNA splicing Source: SGD

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34303-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    U4/U6 small nuclear ribonucleoprotein PRP4
    Alternative name(s):
    Pre-mRNA-processing protein 4
    Gene namesi
    Name:PRP4
    Synonyms:RNA4
    Ordered Locus Names:YPR178W
    ORF Names:P9705.6
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR178w.
    SGDiS000006382. PRP4.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. spliceosomal complex Source: UniProtKB-KW
    2. U4/U6 x U5 tri-snRNP complex Source: SGD

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 465465U4/U6 small nuclear ribonucleoprotein PRP4PRO_0000051152Add
    BLAST

    Proteomic databases

    MaxQBiP20053.
    PaxDbiP20053.

    Expressioni

    Gene expression databases

    GenevestigatoriP20053.

    Interactioni

    Subunit structurei

    Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LSM7P539053EBI-219,EBI-141
    LSM8P470933EBI-219,EBI-313
    PRP3Q033385EBI-219,EBI-421

    Protein-protein interaction databases

    BioGridi36350. 309 interactions.
    DIPiDIP-2607N.
    IntActiP20053. 29 interactions.
    MINTiMINT-423902.
    STRINGi4932.YPR178W.

    Structurei

    3D structure databases

    ProteinModelPortaliP20053.
    SMRiP20053. Positions 130-465.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati173 – 21240WD 1Add
    BLAST
    Repeati216 – 25641WD 2Add
    BLAST
    Repeati263 – 30240WD 3Add
    BLAST
    Repeati305 – 34440WD 4Add
    BLAST
    Repeati347 – 38640WD 5Add
    BLAST
    Repeati391 – 43242WD 6Add
    BLAST
    Repeati435 – 46430WD 7Add
    BLAST

    Sequence similaritiesi

    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00740000115057.
    HOGENOMiHOG000157615.
    KOiK12662.
    OMAiHPSRQAY.
    OrthoDBiEOG7H4F3W.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR027106. Prp4.
    IPR003648. SFM.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PANTHERiPTHR19846. PTHR19846. 1 hit.
    PfamiPF00400. WD40. 6 hits.
    [Graphical view]
    PRINTSiPR00320. GPROTEINBRPT.
    SMARTiSM00500. SFM. 1 hit.
    SM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 4 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P20053-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKYIALENL PVDLQHKGAT QNESTADILK QLPHERLQAV LEKIPEEDLE    50
    VRRLLSILKK PEVVENEDVQ QRRIRLAEIL MVDEIDLENI NNMENINGEE 100
    VDEEDDEDFF TPATSELIFA RRFLINYSLE RSRKRLQKEM ERHQKFNTRQ 150
    ELLSRRTELQ RMANLELAGS QLVSTKPISA VSLSTDDMVV ATGSWAGDLQ 200
    VLNSQTLQPL TQKLDSHVGK IGAIDWHPDS NNQMISCAED GLIKNFQYSN 250
    EEGGLRLLGD LVGHERRISD VKYHPSGKFI GSASHDMTWR LWDASTHQEL 300
    LLQEGHDKGV FSLSFQCDGS LVCSGGMDSL SMLWDIRSGS KVMTLAGHSK 350
    PIYTVAWSPN GYQVATGGGD GIINVWDIRK RDEGQLNQIL AHRNIVTQVR 400
    FSKEDGGKKL VSCGYDNLIN VYSSDTWLKM GSLAGHTDKI ISLDISNNSH 450
    FLVSGGWDRS IKLWN 465
    Length:465
    Mass (Da):52,442
    Last modified:February 1, 1991 - v1
    Checksum:i7CCCA3A92AC0CE78
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26597 Genomic DNA. Translation: AAA79332.1.
    M28518 Genomic DNA. Translation: AAA79011.1.
    U25842 Genomic DNA. Translation: AAB68111.1.
    BK006949 Genomic DNA. Translation: DAA11594.1.
    PIRiA32569.
    RefSeqiNP_015504.1. NM_001184275.1.

    Genome annotation databases

    EnsemblFungiiYPR178W; YPR178W; YPR178W.
    GeneIDi856307.
    KEGGisce:YPR178W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26597 Genomic DNA. Translation: AAA79332.1 .
    M28518 Genomic DNA. Translation: AAA79011.1 .
    U25842 Genomic DNA. Translation: AAB68111.1 .
    BK006949 Genomic DNA. Translation: DAA11594.1 .
    PIRi A32569.
    RefSeqi NP_015504.1. NM_001184275.1.

    3D structure databases

    ProteinModelPortali P20053.
    SMRi P20053. Positions 130-465.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36350. 309 interactions.
    DIPi DIP-2607N.
    IntActi P20053. 29 interactions.
    MINTi MINT-423902.
    STRINGi 4932.YPR178W.

    Proteomic databases

    MaxQBi P20053.
    PaxDbi P20053.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR178W ; YPR178W ; YPR178W .
    GeneIDi 856307.
    KEGGi sce:YPR178W.

    Organism-specific databases

    CYGDi YPR178w.
    SGDi S000006382. PRP4.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00740000115057.
    HOGENOMi HOG000157615.
    KOi K12662.
    OMAi HPSRQAY.
    OrthoDBi EOG7H4F3W.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34303-MONOMER.

    Miscellaneous databases

    NextBioi 981676.
    PROi P20053.

    Gene expression databases

    Genevestigatori P20053.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR020472. G-protein_beta_WD-40_rep.
    IPR027106. Prp4.
    IPR003648. SFM.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    PANTHERi PTHR19846. PTHR19846. 1 hit.
    Pfami PF00400. WD40. 6 hits.
    [Graphical view ]
    PRINTSi PR00320. GPROTEINBRPT.
    SMARTi SM00500. SFM. 1 hit.
    SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 4 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle."
      Petersen-Bjoern S., Soltyk A., Beggs J.D., Friesen J.D.
      Mol. Cell. Biol. 9:3698-3709(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle."
      Banroques J., Abelson J.N.
      Mol. Cell. Biol. 9:3710-3719(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."
      Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P.
      EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPRP4_YEAST
    AccessioniPrimary (citable) accession number: P20053
    Secondary accession number(s): D6W4H8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1770 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3