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P20053 (PRP4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
U4/U6 small nuclear ribonucleoprotein PRP4
Alternative name(s):
Pre-mRNA-processing protein 4
Gene names
Name:PRP4
Synonyms:RNA4
Ordered Locus Names:YPR178W
ORF Names:P9705.6
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in RNA splicing. Is required for the association of U4/U6 snRNP with U5 snRNP in an early step of spliceosome assembly.

Subunit structure

Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1. Ref.5

Subcellular location

Nucleus Probable.

Miscellaneous

Present with 1770 molecules/cell in log phase SD medium.

Sequence similarities

Contains 7 WD repeats.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 465465U4/U6 small nuclear ribonucleoprotein PRP4
PRO_0000051152

Regions

Repeat173 – 21240WD 1
Repeat216 – 25641WD 2
Repeat263 – 30240WD 3
Repeat305 – 34440WD 4
Repeat347 – 38640WD 5
Repeat391 – 43242WD 6
Repeat435 – 46430WD 7

Sequences

Sequence LengthMass (Da)Tools
P20053 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 7CCCA3A92AC0CE78

FASTA46552,442
        10         20         30         40         50         60 
MSKYIALENL PVDLQHKGAT QNESTADILK QLPHERLQAV LEKIPEEDLE VRRLLSILKK 

        70         80         90        100        110        120 
PEVVENEDVQ QRRIRLAEIL MVDEIDLENI NNMENINGEE VDEEDDEDFF TPATSELIFA 

       130        140        150        160        170        180 
RRFLINYSLE RSRKRLQKEM ERHQKFNTRQ ELLSRRTELQ RMANLELAGS QLVSTKPISA 

       190        200        210        220        230        240 
VSLSTDDMVV ATGSWAGDLQ VLNSQTLQPL TQKLDSHVGK IGAIDWHPDS NNQMISCAED 

       250        260        270        280        290        300 
GLIKNFQYSN EEGGLRLLGD LVGHERRISD VKYHPSGKFI GSASHDMTWR LWDASTHQEL 

       310        320        330        340        350        360 
LLQEGHDKGV FSLSFQCDGS LVCSGGMDSL SMLWDIRSGS KVMTLAGHSK PIYTVAWSPN 

       370        380        390        400        410        420 
GYQVATGGGD GIINVWDIRK RDEGQLNQIL AHRNIVTQVR FSKEDGGKKL VSCGYDNLIN 

       430        440        450        460 
VYSSDTWLKM GSLAGHTDKI ISLDISNNSH FLVSGGWDRS IKLWN 

« Hide

References

« Hide 'large scale' references
[1]"PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle."
Petersen-Bjoern S., Soltyk A., Beggs J.D., Friesen J.D.
Mol. Cell. Biol. 9:3698-3709(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle."
Banroques J., Abelson J.N.
Mol. Cell. Biol. 9:3710-3719(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."
Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P.
EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M26597 Genomic DNA. Translation: AAA79332.1.
M28518 Genomic DNA. Translation: AAA79011.1.
U25842 Genomic DNA. Translation: AAB68111.1.
BK006949 Genomic DNA. Translation: DAA11594.1.
PIRA32569.
RefSeqNP_015504.1. NM_001184275.1.

3D structure databases

ProteinModelPortalP20053.
SMRP20053. Positions 130-465.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36350. 309 interactions.
DIPDIP-2607N.
IntActP20053. 29 interactions.
MINTMINT-423902.
STRING4932.YPR178W.

Proteomic databases

PaxDbP20053.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR178W; YPR178W; YPR178W.
GeneID856307.
KEGGsce:YPR178W.

Organism-specific databases

CYGDYPR178w.
SGDS000006382. PRP4.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00740000115057.
HOGENOMHOG000157615.
KOK12662.
OMAHPSRQAY.
OrthoDBEOG7H4F3W.

Enzyme and pathway databases

BioCycYEAST:G3O-34303-MONOMER.

Gene expression databases

GenevestigatorP20053.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR020472. G-protein_beta_WD-40_rep.
IPR027106. Prp4.
IPR003648. SFM.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERPTHR19846. PTHR19846. 1 hit.
PfamPF00400. WD40. 6 hits.
[Graphical view]
PRINTSPR00320. GPROTEINBRPT.
SMARTSM00500. SFM. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981676.
PROP20053.

Entry information

Entry namePRP4_YEAST
AccessionPrimary (citable) accession number: P20053
Secondary accession number(s): D6W4H8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: April 16, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families