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Protein

U4/U6 small nuclear ribonucleoprotein PRP4

Gene

PRP4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in RNA splicing. Is required for the association of U4/U6 snRNP with U5 snRNP in an early step of spliceosome assembly.

Miscellaneous

Present with 1770 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
  • snoRNA splicing Source: SGD

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-34303-MONOMER
ReactomeiR-SCE-6781823 Formation of TC-NER Pre-Incision Complex
R-SCE-6782135 Dual incision in TC-NER
R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6 small nuclear ribonucleoprotein PRP4
Alternative name(s):
Pre-mRNA-processing protein 4
Gene namesi
Name:PRP4
Synonyms:RNA4
Ordered Locus Names:YPR178W
ORF Names:P9705.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR178W
SGDiS000006382 PRP4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000511521 – 465U4/U6 small nuclear ribonucleoprotein PRP4Add BLAST465

Proteomic databases

MaxQBiP20053
PaxDbiP20053
PRIDEiP20053

PTM databases

iPTMnetiP20053

Interactioni

Subunit structurei

Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36350, 540 interactors
DIPiDIP-2607N
IntActiP20053, 46 interactors
MINTiP20053
STRINGi4932.YPR178W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCMelectron microscopy3.80B1-465[»]
5GANelectron microscopy3.60H1-465[»]
5GAPelectron microscopy3.60H1-465[»]
5NRLelectron microscopy7.20H1-465[»]
ProteinModelPortaliP20053
SMRiP20053
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati173 – 212WD 1Add BLAST40
Repeati216 – 256WD 2Add BLAST41
Repeati263 – 302WD 3Add BLAST40
Repeati305 – 344WD 4Add BLAST40
Repeati347 – 386WD 5Add BLAST40
Repeati391 – 432WD 6Add BLAST42
Repeati435 – 464WD 7Add BLAST30

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00900000140925
HOGENOMiHOG000157615
InParanoidiP20053
KOiK12662
OMAiDSSWRLW
OrthoDBiEOG092C10HM

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR027106 Prp4
IPR014906 PRP4-like
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19846 PTHR19846, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 4 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00500 SFM, 1 hit
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 3 hits
PS50082 WD_REPEATS_2, 4 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

P20053-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKYIALENL PVDLQHKGAT QNESTADILK QLPHERLQAV LEKIPEEDLE
60 70 80 90 100
VRRLLSILKK PEVVENEDVQ QRRIRLAEIL MVDEIDLENI NNMENINGEE
110 120 130 140 150
VDEEDDEDFF TPATSELIFA RRFLINYSLE RSRKRLQKEM ERHQKFNTRQ
160 170 180 190 200
ELLSRRTELQ RMANLELAGS QLVSTKPISA VSLSTDDMVV ATGSWAGDLQ
210 220 230 240 250
VLNSQTLQPL TQKLDSHVGK IGAIDWHPDS NNQMISCAED GLIKNFQYSN
260 270 280 290 300
EEGGLRLLGD LVGHERRISD VKYHPSGKFI GSASHDMTWR LWDASTHQEL
310 320 330 340 350
LLQEGHDKGV FSLSFQCDGS LVCSGGMDSL SMLWDIRSGS KVMTLAGHSK
360 370 380 390 400
PIYTVAWSPN GYQVATGGGD GIINVWDIRK RDEGQLNQIL AHRNIVTQVR
410 420 430 440 450
FSKEDGGKKL VSCGYDNLIN VYSSDTWLKM GSLAGHTDKI ISLDISNNSH
460
FLVSGGWDRS IKLWN
Length:465
Mass (Da):52,442
Last modified:February 1, 1991 - v1
Checksum:i7CCCA3A92AC0CE78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26597 Genomic DNA Translation: AAA79332.1
M28518 Genomic DNA Translation: AAA79011.1
U25842 Genomic DNA Translation: AAB68111.1
BK006949 Genomic DNA Translation: DAA11594.1
PIRiA32569
RefSeqiNP_015504.1, NM_001184275.1

Genome annotation databases

EnsemblFungiiYPR178W; YPR178W; YPR178W
GeneIDi856307
KEGGisce:YPR178W

Similar proteinsi

Entry informationi

Entry nameiPRP4_YEAST
AccessioniPrimary (citable) accession number: P20053
Secondary accession number(s): D6W4H8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: May 23, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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