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P20053

- PRP4_YEAST

UniProt

P20053 - PRP4_YEAST

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Protein

U4/U6 small nuclear ribonucleoprotein PRP4

Gene
PRP4, RNA4, YPR178W, P9705.6
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in RNA splicing. Is required for the association of U4/U6 snRNP with U5 snRNP in an early step of spliceosome assembly.

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-34303-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6 small nuclear ribonucleoprotein PRP4
Alternative name(s):
Pre-mRNA-processing protein 4
Gene namesi
Name:PRP4
Synonyms:RNA4
Ordered Locus Names:YPR178W
ORF Names:P9705.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR178w.
SGDiS000006382. PRP4.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. spliceosomal complex Source: UniProtKB-KW
  2. U4/U6 x U5 tri-snRNP complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 465465U4/U6 small nuclear ribonucleoprotein PRP4PRO_0000051152Add
BLAST

Proteomic databases

MaxQBiP20053.
PaxDbiP20053.

Expressioni

Gene expression databases

GenevestigatoriP20053.

Interactioni

Subunit structurei

Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
LSM7P539053EBI-219,EBI-141
LSM8P470933EBI-219,EBI-313
PRP3Q033385EBI-219,EBI-421

Protein-protein interaction databases

BioGridi36350. 309 interactions.
DIPiDIP-2607N.
IntActiP20053. 29 interactions.
MINTiMINT-423902.
STRINGi4932.YPR178W.

Structurei

3D structure databases

ProteinModelPortaliP20053.
SMRiP20053. Positions 130-465.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati173 – 21240WD 1Add
BLAST
Repeati216 – 25641WD 2Add
BLAST
Repeati263 – 30240WD 3Add
BLAST
Repeati305 – 34440WD 4Add
BLAST
Repeati347 – 38640WD 5Add
BLAST
Repeati391 – 43242WD 6Add
BLAST
Repeati435 – 46430WD 7Add
BLAST

Sequence similaritiesi

Contains 7 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00740000115057.
HOGENOMiHOG000157615.
KOiK12662.
OMAiHPSRQAY.
OrthoDBiEOG7H4F3W.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR027106. Prp4.
IPR003648. SFM.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19846. PTHR19846. 1 hit.
PfamiPF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00500. SFM. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20053-1 [UniParc]FASTAAdd to Basket

« Hide

MSKYIALENL PVDLQHKGAT QNESTADILK QLPHERLQAV LEKIPEEDLE    50
VRRLLSILKK PEVVENEDVQ QRRIRLAEIL MVDEIDLENI NNMENINGEE 100
VDEEDDEDFF TPATSELIFA RRFLINYSLE RSRKRLQKEM ERHQKFNTRQ 150
ELLSRRTELQ RMANLELAGS QLVSTKPISA VSLSTDDMVV ATGSWAGDLQ 200
VLNSQTLQPL TQKLDSHVGK IGAIDWHPDS NNQMISCAED GLIKNFQYSN 250
EEGGLRLLGD LVGHERRISD VKYHPSGKFI GSASHDMTWR LWDASTHQEL 300
LLQEGHDKGV FSLSFQCDGS LVCSGGMDSL SMLWDIRSGS KVMTLAGHSK 350
PIYTVAWSPN GYQVATGGGD GIINVWDIRK RDEGQLNQIL AHRNIVTQVR 400
FSKEDGGKKL VSCGYDNLIN VYSSDTWLKM GSLAGHTDKI ISLDISNNSH 450
FLVSGGWDRS IKLWN 465
Length:465
Mass (Da):52,442
Last modified:February 1, 1991 - v1
Checksum:i7CCCA3A92AC0CE78
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26597 Genomic DNA. Translation: AAA79332.1.
M28518 Genomic DNA. Translation: AAA79011.1.
U25842 Genomic DNA. Translation: AAB68111.1.
BK006949 Genomic DNA. Translation: DAA11594.1.
PIRiA32569.
RefSeqiNP_015504.1. NM_001184275.1.

Genome annotation databases

EnsemblFungiiYPR178W; YPR178W; YPR178W.
GeneIDi856307.
KEGGisce:YPR178W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26597 Genomic DNA. Translation: AAA79332.1 .
M28518 Genomic DNA. Translation: AAA79011.1 .
U25842 Genomic DNA. Translation: AAB68111.1 .
BK006949 Genomic DNA. Translation: DAA11594.1 .
PIRi A32569.
RefSeqi NP_015504.1. NM_001184275.1.

3D structure databases

ProteinModelPortali P20053.
SMRi P20053. Positions 130-465.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36350. 309 interactions.
DIPi DIP-2607N.
IntActi P20053. 29 interactions.
MINTi MINT-423902.
STRINGi 4932.YPR178W.

Proteomic databases

MaxQBi P20053.
PaxDbi P20053.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR178W ; YPR178W ; YPR178W .
GeneIDi 856307.
KEGGi sce:YPR178W.

Organism-specific databases

CYGDi YPR178w.
SGDi S000006382. PRP4.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00740000115057.
HOGENOMi HOG000157615.
KOi K12662.
OMAi HPSRQAY.
OrthoDBi EOG7H4F3W.

Enzyme and pathway databases

BioCyci YEAST:G3O-34303-MONOMER.

Miscellaneous databases

NextBioi 981676.
PROi P20053.

Gene expression databases

Genevestigatori P20053.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR020472. G-protein_beta_WD-40_rep.
IPR027106. Prp4.
IPR003648. SFM.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
PANTHERi PTHR19846. PTHR19846. 1 hit.
Pfami PF00400. WD40. 6 hits.
[Graphical view ]
PRINTSi PR00320. GPROTEINBRPT.
SMARTi SM00500. SFM. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle."
    Petersen-Bjoern S., Soltyk A., Beggs J.D., Friesen J.D.
    Mol. Cell. Biol. 9:3698-3709(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle."
    Banroques J., Abelson J.N.
    Mol. Cell. Biol. 9:3710-3719(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."
    Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P.
    EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPRP4_YEAST
AccessioniPrimary (citable) accession number: P20053
Secondary accession number(s): D6W4H8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 11, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1770 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

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