Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

U4/U6 small nuclear ribonucleoprotein PRP4

Gene

PRP4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in RNA splicing. Is required for the association of U4/U6 snRNP with U5 snRNP in an early step of spliceosome assembly.

Miscellaneous

Present with 1770 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
  • snoRNA splicing Source: SGD

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-34303-MONOMER.
ReactomeiR-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SCE-77595. Processing of Intronless Pre-mRNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6 small nuclear ribonucleoprotein PRP4
Alternative name(s):
Pre-mRNA-processing protein 4
Gene namesi
Name:PRP4
Synonyms:RNA4
Ordered Locus Names:YPR178W
ORF Names:P9705.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR178W.
SGDiS000006382. PRP4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000511521 – 465U4/U6 small nuclear ribonucleoprotein PRP4Add BLAST465

Proteomic databases

MaxQBiP20053.
PRIDEiP20053.

PTM databases

iPTMnetiP20053.

Interactioni

Subunit structurei

Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36350. 537 interactors.
DIPiDIP-2607N.
IntActiP20053. 57 interactors.
MINTiMINT-423902.
STRINGi4932.YPR178W.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCMelectron microscopy3.80B1-465[»]
5GANelectron microscopy3.60H1-465[»]
5GAPelectron microscopy3.60H1-465[»]
5NRLelectron microscopy7.20H1-465[»]
ProteinModelPortaliP20053.
SMRiP20053.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati173 – 212WD 1Add BLAST40
Repeati216 – 256WD 2Add BLAST41
Repeati263 – 302WD 3Add BLAST40
Repeati305 – 344WD 4Add BLAST40
Repeati347 – 386WD 5Add BLAST40
Repeati391 – 432WD 6Add BLAST42
Repeati435 – 464WD 7Add BLAST30

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00810000125363.
HOGENOMiHOG000157615.
InParanoidiP20053.
KOiK12662.
OMAiDSSWRLW.
OrthoDBiEOG092C10HM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR020472. G-protein_beta_WD-40_rep.
IPR014906. PRP4-like.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
PfamiView protein in Pfam
PF00400. WD40. 4 hits.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiView protein in SMART
SM00500. SFM. 1 hit.
SM00320. WD40. 7 hits.
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiView protein in PROSITE
PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.

Sequencei

Sequence statusi: Complete.

P20053-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKYIALENL PVDLQHKGAT QNESTADILK QLPHERLQAV LEKIPEEDLE
60 70 80 90 100
VRRLLSILKK PEVVENEDVQ QRRIRLAEIL MVDEIDLENI NNMENINGEE
110 120 130 140 150
VDEEDDEDFF TPATSELIFA RRFLINYSLE RSRKRLQKEM ERHQKFNTRQ
160 170 180 190 200
ELLSRRTELQ RMANLELAGS QLVSTKPISA VSLSTDDMVV ATGSWAGDLQ
210 220 230 240 250
VLNSQTLQPL TQKLDSHVGK IGAIDWHPDS NNQMISCAED GLIKNFQYSN
260 270 280 290 300
EEGGLRLLGD LVGHERRISD VKYHPSGKFI GSASHDMTWR LWDASTHQEL
310 320 330 340 350
LLQEGHDKGV FSLSFQCDGS LVCSGGMDSL SMLWDIRSGS KVMTLAGHSK
360 370 380 390 400
PIYTVAWSPN GYQVATGGGD GIINVWDIRK RDEGQLNQIL AHRNIVTQVR
410 420 430 440 450
FSKEDGGKKL VSCGYDNLIN VYSSDTWLKM GSLAGHTDKI ISLDISNNSH
460
FLVSGGWDRS IKLWN
Length:465
Mass (Da):52,442
Last modified:February 1, 1991 - v1
Checksum:i7CCCA3A92AC0CE78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26597 Genomic DNA. Translation: AAA79332.1.
M28518 Genomic DNA. Translation: AAA79011.1.
U25842 Genomic DNA. Translation: AAB68111.1.
BK006949 Genomic DNA. Translation: DAA11594.1.
PIRiA32569.
RefSeqiNP_015504.1. NM_001184275.1.

Genome annotation databases

EnsemblFungiiYPR178W; YPR178W; YPR178W.
GeneIDi856307.
KEGGisce:YPR178W.

Similar proteinsi

Entry informationi

Entry nameiPRP4_YEAST
AccessioniPrimary (citable) accession number: P20053
Secondary accession number(s): D6W4H8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: September 27, 2017
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names