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Protein

PHO85 cyclin PHO80

Gene

PHO80

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cyclin partner of the cyclin-dependent kinase (CDK) PHO85. Negatively regulates the expression of phosphate-starvation-responsive genes under phosphate-rich conditions. The PHO80-PHO85 cyclin-CDK holoenzyme phosphorylates and inactivates the transcription factor PHO4, by preventing its association with the transcription factor PHO2 and the nuclear import receptor PSE1, and by promoting association with the nuclear export receptor MSN5, excluding PHO4 from the nucleus. PHO80-PHO85 phosphorylates and inactivates protein kinase RIM15 by retaining it in the cytoplasm, antagonizing RIM15-induced entry into stationary phase. PHO80-PHO85 also phosphorylates and inactivates the calcineurin-responsive transcription factor CRZ1, linking PHO85 to calcium signaling.6 Publications

Enzyme regulationi

Inhibited by the CDK inhibitor (CKI) PHO81 in response to phosphate starvation.1 Publication

GO - Molecular functioni

  • cyclin-dependent protein serine/threonine kinase regulator activity Source: SGD

GO - Biological processi

  • cellular metal ion homeostasis Source: SGD
  • negative regulation of calcium-mediated signaling Source: SGD
  • negative regulation of macroautophagy Source: SGD
  • negative regulation of phosphate metabolic process Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD
  • regulation of protein localization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Enzyme and pathway databases

BioCyciYEAST:G3O-33418-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
PHO85 cyclin PHO80
Alternative name(s):
Aminoglycoside antibiotic sensitivity protein 3
Phosphate system cyclin PHO80
Gene namesi
Name:PHO80
Synonyms:AGS3, TUP7, VAC5
Ordered Locus Names:YOL001W
ORF Names:O2505, UNB293
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL001W.
SGDiS000005361. PHO80.

Subcellular locationi

GO - Cellular componenti

  • cyclin-dependent protein kinase holoenzyme complex Source: SGD
  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: SGD
  • Pho85-Pho80 CDK-cyclin complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi163L → S: Temperature-sensitive allele. 1 Publication1
Mutagenesisi229G → D in PHO80-1. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2111355.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805021 – 293PHO85 cyclin PHO80Add BLAST293

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei234Phosphoserine; by PHO851 Publication1
Modified residuei267Phosphoserine; by PHO851 Publication1

Post-translational modificationi

Phosphorylation of Ser-267 by PHO85 is required to form an active cyclin-kinase complex and for function.5 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP20052.
PRIDEiP20052.

PTM databases

iPTMnetiP20052.

Interactioni

Subunit structurei

Forms a cyclin-CDK complex with PHO85. PHO80-PHO85 forms a stable complex with its inhibitor PHO81 under both high- and low-phosphate conditions, but PHO81 only inhibits the kinase upon phosphate starvation. Interacts with transcription factor PHO4.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PHO85P171574EBI-13310,EBI-13327

Protein-protein interaction databases

BioGridi34403. 288 interactors.
DIPiDIP-2479N.
IntActiP20052. 8 interactors.
MINTiMINT-2786576.

Chemistry databases

BindingDBiP20052.

Structurei

Secondary structure

1293
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi20 – 22Combined sources3
Helixi27 – 29Combined sources3
Helixi32 – 51Combined sources20
Helixi77 – 86Combined sources10
Turni87 – 89Combined sources3
Helixi92 – 108Combined sources17
Turni116 – 118Combined sources3
Helixi119 – 134Combined sources16
Helixi141 – 148Combined sources8
Helixi152 – 164Combined sources13
Turni165 – 168Combined sources4
Helixi176 – 183Combined sources8
Helixi210 – 213Combined sources4
Helixi216 – 227Combined sources12
Helixi235 – 237Combined sources3
Beta strandi242 – 244Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PK9X-ray2.91B/D1-293[»]
2PMIX-ray2.90B/D1-293[»]
ProteinModelPortaliP20052.
SMRiP20052.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20052.

Family & Domainsi

Sequence similaritiesi

Belongs to the cyclin family. PHO80 subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00530000066532.
HOGENOMiHOG000000777.
InParanoidiP20052.
KOiK06654.
OMAiSSLEHCV.
OrthoDBiEOG092C4D3O.

Family and domain databases

InterProiIPR013922. Cyclin_PHO80-like.
[Graphical view]
PfamiPF08613. Cyclin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20052-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESTSGERSE NIHEDQGIPK VILPADFNKC SRTDLVVLIS RMLVSLIAIN
60 70 80 90 100
ENSATKKSDD QITLTRYHSK IPPNISIFNY FIRLTKFSSL EHCVLMTSLY
110 120 130 140 150
YIDLLQTVYP DFTLNSLTAH RFLLTATTVA TKGLCDSFST NAHYAKVGGV
160 170 180 190 200
RCHELNILEN DFLKRVNYRI IPRDHNITLC SIEQKQKKFV IDKNALGSLD
210 220 230 240 250
LDSYSYVNRP KSGYNVLDKY YRRIVQLVGS FNASPDKSRK VDYVLPPNID
260 270 280 290
IVSESGSQTT QLKGSSSPNS HSSQKRYSEA KDAHIYNKRS KPD
Length:293
Mass (Da):33,227
Last modified:July 5, 2005 - v3
Checksum:i72918CAF845AE74C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65T → S in CAA30347 (PubMed:3283704).Curated1
Sequence conflicti247P → S in AAA34869 (PubMed:2269431).Curated1
Sequence conflicti247P → S in AAA34870 (PubMed:2269431).Curated1
Sequence conflicti254E → K in AAA34869 (PubMed:2269431).Curated1
Sequence conflicti254E → K in AAA34870 (PubMed:2269431).Curated1
Sequence conflicti292P → A in CAA30347 (PubMed:3283704).Curated1
Sequence conflicti292P → A no nucleotide entry (PubMed:3333302).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07464 Genomic DNA. Translation: CAA30347.1.
U43491 Genomic DNA. Translation: AAC49479.1.
Z74743 Genomic DNA. Translation: CAA99000.1.
Y00382 Genomic DNA. Translation: CAA68454.1.
M60624 Genomic DNA. Translation: AAA34869.1.
M60625 Genomic DNA. Translation: AAA34870.1.
BK006948 Genomic DNA. Translation: DAA10782.1.
PIRiS61983.
RefSeqiNP_014642.1. NM_001183255.1.

Genome annotation databases

EnsemblFungiiYOL001W; YOL001W; YOL001W.
GeneIDi854161.
KEGGisce:YOL001W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07464 Genomic DNA. Translation: CAA30347.1.
U43491 Genomic DNA. Translation: AAC49479.1.
Z74743 Genomic DNA. Translation: CAA99000.1.
Y00382 Genomic DNA. Translation: CAA68454.1.
M60624 Genomic DNA. Translation: AAA34869.1.
M60625 Genomic DNA. Translation: AAA34870.1.
BK006948 Genomic DNA. Translation: DAA10782.1.
PIRiS61983.
RefSeqiNP_014642.1. NM_001183255.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PK9X-ray2.91B/D1-293[»]
2PMIX-ray2.90B/D1-293[»]
ProteinModelPortaliP20052.
SMRiP20052.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34403. 288 interactors.
DIPiDIP-2479N.
IntActiP20052. 8 interactors.
MINTiMINT-2786576.

Chemistry databases

BindingDBiP20052.
ChEMBLiCHEMBL2111355.

PTM databases

iPTMnetiP20052.

Proteomic databases

MaxQBiP20052.
PRIDEiP20052.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL001W; YOL001W; YOL001W.
GeneIDi854161.
KEGGisce:YOL001W.

Organism-specific databases

EuPathDBiFungiDB:YOL001W.
SGDiS000005361. PHO80.

Phylogenomic databases

GeneTreeiENSGT00530000066532.
HOGENOMiHOG000000777.
InParanoidiP20052.
KOiK06654.
OMAiSSLEHCV.
OrthoDBiEOG092C4D3O.

Enzyme and pathway databases

BioCyciYEAST:G3O-33418-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP20052.
PROiP20052.

Family and domain databases

InterProiIPR013922. Cyclin_PHO80-like.
[Graphical view]
PfamiPF08613. Cyclin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHO80_YEAST
AccessioniPrimary (citable) accession number: P20052
Secondary accession number(s): D6W266, Q06882, Q06883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 5, 2005
Last modified: November 2, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.