Reviewed, UniProtKB/Swiss-Prot P20049 (TYR1_YEAST)

Names and origin

Protein names

Recommended name:
    Prephenate dehydrogenase [NADP+]
      Short name=PRDH
    EC=1.3.1.13

Gene names

Name:	TYR1
Ordered Locus Names:	YBR166C
ORF Names:	YBR1218

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Prephenate + NADP+ = 4-hydroxyphenylpyruvate + CO2 + NADPH.
Pathway	Amino-acid biosynthesis; L-tyrosine biosynthesis; L-tyrosine from prephenate: step 1/2.
Induction	Presence of Phe represses the TYR1 gene expression.
Miscellaneous	Present with 2180 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Contains 1 prephenate/arogenate dehydrogenase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tyrosine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct prephenate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-19686,EBI-19686

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Prephenate dehydrogenase [NADP+]		PRO_0000119208
Regions
	Domain	14 – 297	284	Prephenate/arogenate dehydrogenase
	Nucleotide binding	14 – 43	30	NADP Potential
Experimental info
	Sequence conflict	38	1	G → S Ref.1
	Sequence conflict	204 – 211	8	IKIYPWTL → DKDSSLDS Ref.1
	Sequence conflict	312 – 313	2	GN → SD Ref.1
	Sequence conflict	433 – 452	20	MIKTI…EKRNT → DDKNHSESF Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P20049-1 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: D483B93ABBC8F8F4	FASTA	452	50,923
	10 20 30 40 50 60 MVSEDKIEQW KATKVIGIIG LGDMGLLYAN KFTDAGWGVI CCDREEYYDE LKEKYASAKF 70 80 90 100 110 120 ELVKNGHLVS RQSDYIIYSV EASNISKIVA TYGPSSKVGT IVGGQTSCKL PEIEAFEKYL 130 140 150 160 170 180 PKDCDIITVH SLHGPKVNTE GQPLVIINHR SQYPESFEFV NSVMACLKSK QVYLTYEEHD 190 200 210 220 230 240 KITADTQAVT HAAFLSMGSA WAKIKIYPWT LGVNKWYGGL ENVKVNISLR IYSNKWHVYA 250 260 270 280 290 300 GLAITNPSAH QQILQYATSA TELFSLMIDN KEQELTDRLL KAKQFVFGKH TGLLLLDDTI 310 320 330 340 350 360 LEKYSLSKSS IGNSNNCKPV PNSHLSLLAI VDSWFQLGID PYDHMICSTP LFRIFLGVSE 370 380 390 400 410 420 YLFLKPGLLE QTIDAAIHDK SFIKDDLEFV ISAREWSSVV SFANFDIYKK QFQSVQKFFE 430 440 450 PMLPEANLIG NEMIKTILSH SSDRSAAEKR NT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the prephenate dehydrogenase-encoding gene, TYR1, from Saccharomyces cerevisiae." Mannhaupt G., Stucka R., Pilz U., Schwarzlose C., Feldmann H. Gene 85:303-311(1989) [PubMed: 2697638] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z36035 Genomic DNA. Translation: CAA85127.1.
PIR	S46037.
RefSeq	NP_009725.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4627N.
IntAct	P20049. 12 interactions.
Proteomic databases
PeptideAtlas	P20049.
Genome annotation databases
Ensembl	YBR166C. Saccharomyces cerevisiae. [Contig view]
GeneID	852464.
GenomeReviews	Gene locus YBR166C in contig Y13134_GR.
KEGG	sce:YBR166C.
NMPDR	fig|4932.3.peg.431.
Organism-specific databases
CYGD	YBR166c.
SGD	S000000370. TYR1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P20049.
OMA	P20049. HVYAGLA.
Enzyme and pathway databases
BRENDA	1.3.1.13. 250.
Gene expression databases
GermOnline	YBR166C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003099. Prephen_DH. IPR012385. Prephenate_DH_fun. [Graphical view]
PIRSF	PIRSF036510. PDH_fung. 1 hit.
PROSITE	PS51176. PDH_ADH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	971408.

Entry information

Entry name

TYR1_YEAST

Accession

Primary (citable) accession number: P20049

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)