P20049 (TYR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prephenate dehydrogenase [NADP+]
Short name=PRDH
EC=1.3.1.13

Gene names

Name:	TYR1
Ordered Locus Names:	YBR166C
ORF Names:	YBR1218

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Prephenate + NADP+ = 4-hydroxyphenylpyruvate + CO2 + NADPH.
Pathway	Amino-acid biosynthesis; L-tyrosine biosynthesis; (4-hydroxyphenyl)pyruvate from prephenate (NADP(+) route): step 1/1.
Induction	Presence of Phe represses the TYR1 gene expression.
Miscellaneous	Present with 2180 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 1 prephenate/arogenate dehydrogenase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tyrosine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tyrosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro prephenate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC prephenate dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-19686,EBI-19686

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Prephenate dehydrogenase [NADP+]		PRO_0000119208
Regions
	Domain	14 – 297	284	Prephenate/arogenate dehydrogenase
	Nucleotide binding	14 – 43	30	NADP Potential
Experimental info
	Sequence conflict	38	1	G → S Ref.1
	Sequence conflict	204 – 211	8	IKIYPWTL → DKDSSLDS Ref.1
	Sequence conflict	312 – 313	2	GN → SD Ref.1
	Sequence conflict	433 – 452	20	MIKTI…EKRNT → DDKNHSESF Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P20049 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: D483B93ABBC8F8F4	FASTA	452	50,923
	10 20 30 40 50 60 MVSEDKIEQW KATKVIGIIG LGDMGLLYAN KFTDAGWGVI CCDREEYYDE LKEKYASAKF 70 80 90 100 110 120 ELVKNGHLVS RQSDYIIYSV EASNISKIVA TYGPSSKVGT IVGGQTSCKL PEIEAFEKYL 130 140 150 160 170 180 PKDCDIITVH SLHGPKVNTE GQPLVIINHR SQYPESFEFV NSVMACLKSK QVYLTYEEHD 190 200 210 220 230 240 KITADTQAVT HAAFLSMGSA WAKIKIYPWT LGVNKWYGGL ENVKVNISLR IYSNKWHVYA 250 260 270 280 290 300 GLAITNPSAH QQILQYATSA TELFSLMIDN KEQELTDRLL KAKQFVFGKH TGLLLLDDTI 310 320 330 340 350 360 LEKYSLSKSS IGNSNNCKPV PNSHLSLLAI VDSWFQLGID PYDHMICSTP LFRIFLGVSE 370 380 390 400 410 420 YLFLKPGLLE QTIDAAIHDK SFIKDDLEFV ISAREWSSVV SFANFDIYKK QFQSVQKFFE 430 440 450 PMLPEANLIG NEMIKTILSH SSDRSAAEKR NT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the prephenate dehydrogenase-encoding gene, TYR1, from Saccharomyces cerevisiae." Mannhaupt G., Stucka R., Pilz U., Schwarzlose C., Feldmann H. Gene 85:303-311(1989) [PubMed: 2697638] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z36035 Genomic DNA. Translation: CAA85127.1. BK006936 Genomic DNA. Translation: DAA07282.1.
PIR	S46037.
RefSeq	NP_009725.1. NM_001178514.1.
3D structure databases
ProteinModelPortal	P20049.
SMR	P20049. Positions 12-82.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4627N.
IntAct	P20049. 13 interactions.
MINT	MINT-542504.
STRING	P20049.
Proteomic databases
PeptideAtlas	P20049.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR166C; YBR166C; YBR166C.
GeneID	852464.
KEGG	sce:YBR166C.
NMPDR	fig|4932.3.peg.431.
Organism-specific databases
CYGD	YBR166c.
SGD	S000000370. TYR1.
Phylogenomic databases
eggNOG	fuNOG05654.
GeneTree	EFGT00050000005741.
HOGENOM	HBG330953.
OMA	HVYAGLA.
OrthoDB	EOG4MGWH6.
Gene expression databases
ArrayExpress	P20049.
Genevestigator	P20049.
GermOnline	YBR166C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR016040. NAD(P)-bd_dom. IPR003099. Prephen_DH. IPR012385. Prephenate_DH_fun. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00211.
Pfam	PF02153. PDH. 1 hit. [Graphical view]
PIRSF	PIRSF036510. PDH_fung. 1 hit.
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
PROSITE	PS51176. PDH_ADH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971408.

Entry information

Entry name

TYR1_YEAST

Accession

Primary (citable) accession number: P20049
Secondary accession number(s): D6VQG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	October 1, 1994
Last modified:	December 14, 2011
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program