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Protein

Prephenate dehydrogenase [NADP(+)]

Gene

TYR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Prephenate + NADP+ = 4-hydroxyphenylpyruvate + CO2 + NADPH.

Pathwayi: L-tyrosine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (4-hydroxyphenyl)pyruvate from prephenate (NADP(+) route).
Proteins known to be involved in this subpathway in this organism are:
  1. Prephenate dehydrogenase [NADP(+)] (TYR1)
This subpathway is part of the pathway L-tyrosine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (4-hydroxyphenyl)pyruvate from prephenate (NADP(+) route), the pathway L-tyrosine biosynthesis and in Amino-acid biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 4330NADPSequence analysisAdd
BLAST

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • NAD+ binding Source: GO_Central
  • prephenate dehydrogenase (NADP+) activity Source: GO_Central
  • prephenate dehydrogenase activity Source: SGD

GO - Biological processi

  • oxidation-reduction process Source: GO_Central
  • tyrosine biosynthetic process Source: GO_Central
  • tyrosine metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tyrosine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciYEAST:YBR166C-MONOMER.
UniPathwayiUPA00122; UER00962.

Names & Taxonomyi

Protein namesi
Recommended name:
Prephenate dehydrogenase [NADP(+)] (EC:1.3.1.13)
Short name:
PRDH
Gene namesi
Name:TYR1
Ordered Locus Names:YBR166C
ORF Names:YBR1218
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR166C.
SGDiS000000370. TYR1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452Prephenate dehydrogenase [NADP(+)]PRO_0000119208Add
BLAST

Proteomic databases

MaxQBiP20049.

Expressioni

Inductioni

Presence of Phe represses the TYR1 gene expression.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-19686,EBI-19686

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi32866. 21 interactions.
DIPiDIP-4627N.
IntActiP20049. 12 interactions.
MINTiMINT-542504.

Structurei

3D structure databases

ProteinModelPortaliP20049.
SMRiP20049. Positions 13-81.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 297284Prephenate/arogenate dehydrogenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 prephenate/arogenate dehydrogenase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000163305.
InParanoidiP20049.
KOiK00211.
OMAiYIIYSVE.
OrthoDBiEOG092C2OAE.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR003099. Prephen_DH.
IPR012385. Prephenate_DH_fun.
[Graphical view]
PfamiPF02153. PDH. 1 hit.
[Graphical view]
PIRSFiPIRSF036510. PDH_fung. 1 hit.
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF51735. SSF51735. 1 hit.
PROSITEiPS51176. PDH_ADH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20049-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSEDKIEQW KATKVIGIIG LGDMGLLYAN KFTDAGWGVI CCDREEYYDE
60 70 80 90 100
LKEKYASAKF ELVKNGHLVS RQSDYIIYSV EASNISKIVA TYGPSSKVGT
110 120 130 140 150
IVGGQTSCKL PEIEAFEKYL PKDCDIITVH SLHGPKVNTE GQPLVIINHR
160 170 180 190 200
SQYPESFEFV NSVMACLKSK QVYLTYEEHD KITADTQAVT HAAFLSMGSA
210 220 230 240 250
WAKIKIYPWT LGVNKWYGGL ENVKVNISLR IYSNKWHVYA GLAITNPSAH
260 270 280 290 300
QQILQYATSA TELFSLMIDN KEQELTDRLL KAKQFVFGKH TGLLLLDDTI
310 320 330 340 350
LEKYSLSKSS IGNSNNCKPV PNSHLSLLAI VDSWFQLGID PYDHMICSTP
360 370 380 390 400
LFRIFLGVSE YLFLKPGLLE QTIDAAIHDK SFIKDDLEFV ISAREWSSVV
410 420 430 440 450
SFANFDIYKK QFQSVQKFFE PMLPEANLIG NEMIKTILSH SSDRSAAEKR

NT
Length:452
Mass (Da):50,923
Last modified:October 1, 1994 - v2
Checksum:iD483B93ABBC8F8F4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 381G → S (PubMed:2697638).Curated
Sequence conflicti204 – 2118IKIYPWTL → DKDSSLDS (PubMed:2697638).Curated
Sequence conflicti312 – 3132GN → SD (PubMed:2697638).Curated
Sequence conflicti433 – 45220MIKTI…EKRNT → DDKNHSESF (PubMed:2697638).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36035 Genomic DNA. Translation: CAA85127.1.
BK006936 Genomic DNA. Translation: DAA07282.1.
PIRiS46037.
RefSeqiNP_009725.1. NM_001178514.1.

Genome annotation databases

EnsemblFungiiYBR166C; YBR166C; YBR166C.
GeneIDi852464.
KEGGisce:YBR166C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36035 Genomic DNA. Translation: CAA85127.1.
BK006936 Genomic DNA. Translation: DAA07282.1.
PIRiS46037.
RefSeqiNP_009725.1. NM_001178514.1.

3D structure databases

ProteinModelPortaliP20049.
SMRiP20049. Positions 13-81.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32866. 21 interactions.
DIPiDIP-4627N.
IntActiP20049. 12 interactions.
MINTiMINT-542504.

Proteomic databases

MaxQBiP20049.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR166C; YBR166C; YBR166C.
GeneIDi852464.
KEGGisce:YBR166C.

Organism-specific databases

EuPathDBiFungiDB:YBR166C.
SGDiS000000370. TYR1.

Phylogenomic databases

HOGENOMiHOG000163305.
InParanoidiP20049.
KOiK00211.
OMAiYIIYSVE.
OrthoDBiEOG092C2OAE.

Enzyme and pathway databases

UniPathwayiUPA00122; UER00962.
BioCyciYEAST:YBR166C-MONOMER.

Miscellaneous databases

PROiP20049.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR003099. Prephen_DH.
IPR012385. Prephenate_DH_fun.
[Graphical view]
PfamiPF02153. PDH. 1 hit.
[Graphical view]
PIRSFiPIRSF036510. PDH_fung. 1 hit.
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF51735. SSF51735. 1 hit.
PROSITEiPS51176. PDH_ADH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYR1_YEAST
AccessioniPrimary (citable) accession number: P20049
Secondary accession number(s): D6VQG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 1, 1994
Last modified: September 7, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2180 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.