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Protein

Dolichol kinase

Gene

SEC59

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the sugar donor Dol-P-Man which is required in the synthesis of N-linked and O-linked oligosaccharides and for that of GPI anchors. It is required for spore germination. Has an essential role in cellular metabolism.

Catalytic activityi

CTP + dolichol = CDP + dolichyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • dolichol kinase activity Source: SGD

GO - Biological processi

  • dolichyl monophosphate biosynthetic process Source: SGD
  • protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-7121.
YEAST:YMR013C-MONOMER.
BRENDAi2.7.1.108. 984.
ReactomeiR-SCE-446199. Synthesis of Dolichyl-phosphate.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichol kinase (EC:2.7.1.108)
Gene namesi
Name:SEC59
Ordered Locus Names:YMR013C
ORF Names:YM8270.17C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR013C.
SGDiS000004615. SEC59.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4747CytoplasmicSequence analysisAdd
BLAST
Transmembranei48 – 6821HelicalSequence analysisAdd
BLAST
Topological domaini69 – 8820LumenalSequence analysisAdd
BLAST
Transmembranei89 – 10921HelicalSequence analysisAdd
BLAST
Topological domaini110 – 1189CytoplasmicSequence analysis
Transmembranei119 – 13921HelicalSequence analysisAdd
BLAST
Topological domaini140 – 15112LumenalSequence analysisAdd
BLAST
Transmembranei152 – 17221HelicalSequence analysisAdd
BLAST
Topological domaini173 – 1819CytoplasmicSequence analysis
Transmembranei182 – 20322HelicalSequence analysisAdd
BLAST
Topological domaini204 – 22320LumenalSequence analysisAdd
BLAST
Transmembranei224 – 24421HelicalSequence analysisAdd
BLAST
Topological domaini245 – 2539CytoplasmicSequence analysis
Transmembranei254 – 27421HelicalSequence analysisAdd
BLAST
Topological domaini275 – 29420LumenalSequence analysisAdd
BLAST
Transmembranei295 – 31521HelicalSequence analysisAdd
BLAST
Topological domaini316 – 32611CytoplasmicSequence analysisAdd
BLAST
Transmembranei327 – 34721HelicalSequence analysisAdd
BLAST
Topological domaini348 – 3492LumenalSequence analysis
Transmembranei350 – 37021HelicalSequence analysisAdd
BLAST
Topological domaini371 – 39424CytoplasmicSequence analysisAdd
BLAST
Transmembranei395 – 41521HelicalSequence analysisAdd
BLAST
Topological domaini416 – 4172LumenalSequence analysis
Transmembranei418 – 43821HelicalSequence analysisAdd
BLAST
Topological domaini439 – 44911CytoplasmicSequence analysisAdd
BLAST
Transmembranei450 – 47021HelicalSequence analysisAdd
BLAST
Topological domaini471 – 4722LumenalSequence analysis
Transmembranei473 – 49321HelicalSequence analysisAdd
BLAST
Topological domaini494 – 51926CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 519519Dolichol kinasePRO_0000097662Add
BLAST

Proteomic databases

MaxQBiP20048.

Interactioni

Protein-protein interaction databases

BioGridi35183. 31 interactions.
DIPiDIP-8198N.

Structurei

3D structure databases

ProteinModelPortaliP20048.
SMRiP20048. Positions 421-471.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polyprenol kinase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000004067.
HOGENOMiHOG000141906.
InParanoidiP20048.
KOiK00902.
OMAiNTSRKIW.
OrthoDBiEOG7KSXJ1.

Family and domain databases

InterProiIPR032974. Polypren_kinase.
[Graphical view]
PANTHERiPTHR13205. PTHR13205. 1 hit.

Sequencei

Sequence statusi: Complete.

P20048-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAIIPHASF TTIKLTQKTE GSQMPTEEIC KINMRTRKFD VGGNSRDFEC
60 70 80 90 100
FYSNFVQTVI LLGTFFYCVE RLQPWSIVTA DISYKQIFVN VFVVCLIMVG
110 120 130 140 150
LIFTKYWQHG YKSLPKFDTI YSLYLPFMVS LLFDTSSTVI NTILILSVLN
160 170 180 190 200
SYRWRTQLVV IILQLCLIFF NFEAGDRLKN IISIVINSLL SLILKYIGQL
210 220 230 240 250
KSLDNIDSNL FSILLTNILY VSEAGTVHFR ILKGIILALT TIISINYVLK
260 270 280 290 300
KVMHFKPFML SISFAIGLPL FANTFIHLED GENPLLWLVK YILESTIRQK
310 320 330 340 350
ILFAWSSILI LSIPSILIEK DSLSLNTSRK LWHFIIFLLI IPSFQMDSNF
360 370 380 390 400
VKIALSGTIP VFLSIEYIRF QNLPPLGSAI ELQLRRFADD RDHSGPLIIS
410 420 430 440 450
YLYLLFGIST PLLMNNSPMG LIGLGIGDSL ASIIGKRYGR IRWKGTQKTL
460 470 480 490 500
EGTLAFIVTS FIVCLVLLRF DKAAIFNHLT TLQLLTLCTL SGVLEGNSVL
510
NDNILIPAFM MICEKLITL
Length:519
Mass (Da):58,906
Last modified:February 1, 1991 - v1
Checksum:i2C75B6F175BBFF06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25779 Genomic DNA. Translation: AAA35033.1.
Z48613 Genomic DNA. Translation: CAA88530.1.
AY692829 Genomic DNA. Translation: AAT92848.1.
BK006946 Genomic DNA. Translation: DAA09911.1.
PIRiJQ0124.
RefSeqiNP_013726.1. NM_001182509.1.

Genome annotation databases

EnsemblFungiiYMR013C; YMR013C; YMR013C.
GeneIDi855026.
KEGGisce:YMR013C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25779 Genomic DNA. Translation: AAA35033.1.
Z48613 Genomic DNA. Translation: CAA88530.1.
AY692829 Genomic DNA. Translation: AAT92848.1.
BK006946 Genomic DNA. Translation: DAA09911.1.
PIRiJQ0124.
RefSeqiNP_013726.1. NM_001182509.1.

3D structure databases

ProteinModelPortaliP20048.
SMRiP20048. Positions 421-471.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35183. 31 interactions.
DIPiDIP-8198N.

Proteomic databases

MaxQBiP20048.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR013C; YMR013C; YMR013C.
GeneIDi855026.
KEGGisce:YMR013C.

Organism-specific databases

EuPathDBiFungiDB:YMR013C.
SGDiS000004615. SEC59.

Phylogenomic databases

GeneTreeiENSGT00390000004067.
HOGENOMiHOG000141906.
InParanoidiP20048.
KOiK00902.
OMAiNTSRKIW.
OrthoDBiEOG7KSXJ1.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:MONOMER-7121.
YEAST:YMR013C-MONOMER.
BRENDAi2.7.1.108. 984.
ReactomeiR-SCE-446199. Synthesis of Dolichyl-phosphate.

Miscellaneous databases

NextBioi978221.
PROiP20048.

Family and domain databases

InterProiIPR032974. Polypren_kinase.
[Graphical view]
PANTHERiPTHR13205. PTHR13205. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sec59 encodes a membrane protein required for core glycosylation in Saccharomyces cerevisiae."
    Bernstein M., Kepes F., Schekman R.
    Mol. Cell. Biol. 9:1191-1199(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Saccharomyces cerevisiae sec59 cells are deficient in dolichol kinase activity."
    Heller L., Orlean P., Adair W.L. Jr.
    Proc. Natl. Acad. Sci. U.S.A. 89:7013-7016(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: ATCC 204508 / S288c.
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiSEC59_YEAST
AccessioniPrimary (citable) accession number: P20048
Secondary accession number(s): D6VZI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: March 16, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.