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Protein

Cadmium resistance transcriptional regulatory protein CadC

Gene

cadC

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Metal-binding repressor for the cad operon. Involved in resistance to heavy metals, such as cadmium, bismuth, zinc or lead. Binds 2 metal ions per subunit. Metal binding to the N-terminal regulatory site causes the repressor to dissociate from the DNA.5 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71Cadmium; shared with dimeric partner
Metal bindingi11 – 111Cadmium; shared with dimeric partner
Metal bindingi58 – 581Cadmium; shared with dimeric partner
Metal bindingi60 – 601Cadmium; shared with dimeric partner
Metal bindingi101 – 1011Zinc; shared with dimeric partner
Metal bindingi103 – 1031Zinc; shared with dimeric partner
Metal bindingi114 – 1141Zinc; shared with dimeric partner
Metal bindingi117 – 1171Zinc; shared with dimeric partner

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi59 – 7820H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cadmium resistance, Transcription, Transcription regulation

Keywords - Ligandi

Cadmium, DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cadmium resistance transcriptional regulatory protein CadC
Alternative name(s):
Cadmium efflux system accessory protein
Gene namesi
Name:cadC
Encoded oniPlasmid pI2580 Publication
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi7 – 71C → S: Abolishes dissociation from DNA; when associated with G-11; S-58 and S-60. 2 Publications
Mutagenesisi11 – 111C → G: No effect. Abolishes dissociation from DNA; when associated with S-7; S-58 and S-60. 2 Publications
Mutagenesisi58 – 581C → S: Abolishes dissociation from DNA; when associated with S-7; G-11 and S-60. 2 Publications
Mutagenesisi60 – 601C → S: Abolishes dissociation from DNA; when associated with S-7; G-11 and S-58. 2 Publications
Mutagenesisi101 – 1011D → G: No effect on repressor activity; when associated with A-103. 1 Publication
Mutagenesisi103 – 1031H → A: No effect on repressor activity; when associated with G-101. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 122122Cadmium resistance transcriptional regulatory protein CadCPRO_0000160620Add
BLAST

Interactioni

Subunit structurei

Homodimer.4 Publications

Structurei

Secondary structure

1
122
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 133Combined sources
Helixi14 – 2512Combined sources
Turni26 – 283Combined sources
Helixi29 – 4012Combined sources
Helixi42 – 5312Combined sources
Helixi59 – 668Combined sources
Helixi70 – 8213Combined sources
Beta strandi85 – 906Combined sources
Beta strandi96 – 1005Combined sources
Helixi102 – 11514Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U2WX-ray1.90A/B/C/D1-122[»]
3F72X-ray2.31A/B/C/D/E/F1-122[»]
ProteinModelPortaliP20047.
SMRiP20047. Positions 12-117.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20047.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 11996HTH arsR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH arsR-type DNA-binding domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR018334. ArsR_HTH.
IPR001845. HTH_ArsR_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01022. HTH_5. 1 hit.
[Graphical view]
PRINTSiPR00778. HTHARSR.
SMARTiSM00418. HTH_ARSR. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00846. HTH_ARSR_1. 1 hit.
PS50987. HTH_ARSR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20047-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKDTCEIF CYDEEKVNRI QGDLQTVDIS GVSQILKAIA DENRAKITYA
60 70 80 90 100
LCQDEELCVC DIANILGVTI ANASHHLRTL YKQGVVNFRK EGKLALYSLG
110 120
DEHIRQIMMI ALAHKKEVKV NV
Length:122
Mass (Da):13,779
Last modified:February 1, 1991 - v1
Checksum:i7C60BBAFFEB91BC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04551 Genomic DNA. Translation: AAB59153.1.
PIRiB32561.
RefSeqiWP_000726007.1. NZ_LFED02000005.1.
YP_006937601.1. NC_013319.1.
YP_006937788.1. NC_013323.1.
YP_006938262.1. NC_013337.1.
YP_006938636.1. NC_013347.1.
YP_006938769.1. NC_013352.1.

Genome annotation databases

GeneIDi13874749.
13874940.
13875429.
13875813.
13875978.
KEGGipg:13874749.
pg:13874940.
pg:13875429.
pg:13875813.
pg:13875978.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04551 Genomic DNA. Translation: AAB59153.1.
PIRiB32561.
RefSeqiWP_000726007.1. NZ_LFED02000005.1.
YP_006937601.1. NC_013319.1.
YP_006937788.1. NC_013323.1.
YP_006938262.1. NC_013337.1.
YP_006938636.1. NC_013347.1.
YP_006938769.1. NC_013352.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U2WX-ray1.90A/B/C/D1-122[»]
3F72X-ray2.31A/B/C/D/E/F1-122[»]
ProteinModelPortaliP20047.
SMRiP20047. Positions 12-117.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13874749.
13874940.
13875429.
13875813.
13875978.
KEGGipg:13874749.
pg:13874940.
pg:13875429.
pg:13875813.
pg:13875978.

Miscellaneous databases

EvolutionaryTraceiP20047.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR018334. ArsR_HTH.
IPR001845. HTH_ArsR_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01022. HTH_5. 1 hit.
[Graphical view]
PRINTSiPR00778. HTHARSR.
SMARTiSM00418. HTH_ARSR. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00846. HTH_ARSR_1. 1 hit.
PS50987. HTH_ARSR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCADC_STAAU
AccessioniPrimary (citable) accession number: P20047
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 6, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The N-terminal, regulatory metal binding site 1 has higher affinity for cadmium than for zinc. The second metal binding site has higher affinity for zinc and has no regulatory function.

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.