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Protein

Eukaryotic translation initiation factor 2 subunit 2

Gene

EIF2S2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri281 – 305C4-typeSequence analysisAdd BLAST25

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • poly(A) RNA binding Source: UniProtKB
  • RNA binding Source: ProtInc
  • translation factor activity, RNA binding Source: ProtInc
  • translation initiation factor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000125977-MONOMER.
ReactomeiR-HSA-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-HSA-382556. ABC-family proteins mediated transport.
R-HSA-72649. Translation initiation complex formation.
R-HSA-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-HSA-72702. Ribosomal scanning and start codon recognition.
R-HSA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-HSA-72731. Recycling of eIF2:GDP.
SIGNORiP20042.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit 2
Alternative name(s):
Eukaryotic translation initiation factor 2 subunit beta
Short name:
eIF-2-beta
Gene namesi
Name:EIF2S2
Synonyms:EIF2B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:3266. EIF2S2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • eukaryotic translation initiation factor 2 complex Source: ProtInc
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi8894.
OpenTargetsiENSG00000125977.
PharmGKBiPA27696.

Polymorphism and mutation databases

BioMutaiEIF2S2.
DMDMi12644154.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001374062 – 333Eukaryotic translation initiation factor 2 subunit 2Add BLAST332

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineCombined sources1
Modified residuei13PhosphoserineCombined sources1
Modified residuei31PhosphothreonineCombined sources1
Modified residuei36PhosphothreonineCombined sources1
Modified residuei67PhosphoserineCombined sources1
Modified residuei105PhosphoserineCombined sources1
Modified residuei111PhosphothreonineCombined sources1
Modified residuei158PhosphoserineCombined sources1
Modified residuei218PhosphoserineBy similarity1
Modified residuei265N6-acetyllysineCombined sources1
Modified residuei293N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP20042.
PaxDbiP20042.
PeptideAtlasiP20042.
PRIDEiP20042.
TopDownProteomicsiP20042.

PTM databases

iPTMnetiP20042.
PhosphoSitePlusiP20042.
SwissPalmiP20042.

Expressioni

Gene expression databases

BgeeiENSG00000125977.
CleanExiHS_EIF2S2.
ExpressionAtlasiP20042. baseline and differential.
GenevisibleiP20042. HS.

Organism-specific databases

HPAiCAB034415.
HPA041262.
HPA041332.

Interactioni

Subunit structurei

Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
EIF2S1P051986EBI-711977,EBI-1056162
EIF2S3P410913EBI-711977,EBI-1054228
EIF4G2P783444EBI-711977,EBI-296519

Protein-protein interaction databases

BioGridi114411. 69 interactors.
IntActiP20042. 28 interactors.
MINTiMINT-5004121.
STRINGi9606.ENSP00000364119.

Structurei

3D structure databases

ProteinModelPortaliP20042.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 21Poly-Lys8
Compositional biasi79 – 87Poly-Lys9
Compositional biasi124 – 129Poly-Lys6

Sequence similaritiesi

Belongs to the eIF-2-beta/eIF-5 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri281 – 305C4-typeSequence analysisAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2768. Eukaryota.
COG1601. LUCA.
GeneTreeiENSGT00390000001804.
HOGENOMiHOG000107198.
HOVERGENiHBG000927.
InParanoidiP20042.
KOiK03238.
OMAiLSFKQMI.
OrthoDBiEOG091G0H6A.
PhylomeDBiP20042.
TreeFamiTF101503.

Family and domain databases

Gene3Di3.30.30.50. 1 hit.
InterProiIPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
[Graphical view]
PfamiPF01873. eIF-5_eIF-2B. 1 hit.
[Graphical view]
SMARTiSM00653. eIF2B_5. 1 hit.
[Graphical view]
SUPFAMiSSF100966. SSF100966. 1 hit.
SSF75689. SSF75689. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20042-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGDEMIFDP TMSKKKKKKK KPFMLDEEGD TQTEETQPSE TKEVEPEPTE
60 70 80 90 100
DKDLEADEED TRKKDASDDL DDLNFFNQKK KKKKTKKIFD IDEAEEGVKD
110 120 130 140 150
LKIESDVQEP TEPEDDLDIM LGNKKKKKKN VKFPDEDEIL EKDEALEDED
160 170 180 190 200
NKKDDGISFS NQTGPAWAGS ERDYTYEELL NRVFNIMREK NPDMVAGEKR
210 220 230 240 250
KFVMKPPQVV RVGTKKTSFV NFTDICKLLH RQPKHLLAFL LAELGTSGSI
260 270 280 290 300
DGNNQLVIKG RFQQKQIENV LRRYIKEYVT CHTCRSPDTI LQKDTRLYFL
310 320 330
QCETCHSRCS VASIKTGFQA VTGKRAQLRA KAN
Length:333
Mass (Da):38,388
Last modified:December 1, 2000 - v2
Checksum:i3E9FD4B48AA61A51
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti295T → I in AAA52383 (PubMed:3044606).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048909177E → D.1 PublicationCorresponds to variant rs17856024dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29536 mRNA. Translation: AAA52383.1.
AL031668 Genomic DNA. Translation: CAB43741.1.
BC000461 mRNA. Translation: AAH00461.1.
BC000934 mRNA. Translation: AAH00934.1.
CCDSiCCDS13231.1.
PIRiA31226.
RefSeqiNP_003899.2. NM_003908.4.
UniGeneiHs.429180.

Genome annotation databases

EnsembliENST00000374980; ENSP00000364119; ENSG00000125977.
GeneIDi8894.
KEGGihsa:8894.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29536 mRNA. Translation: AAA52383.1.
AL031668 Genomic DNA. Translation: CAB43741.1.
BC000461 mRNA. Translation: AAH00461.1.
BC000934 mRNA. Translation: AAH00934.1.
CCDSiCCDS13231.1.
PIRiA31226.
RefSeqiNP_003899.2. NM_003908.4.
UniGeneiHs.429180.

3D structure databases

ProteinModelPortaliP20042.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114411. 69 interactors.
IntActiP20042. 28 interactors.
MINTiMINT-5004121.
STRINGi9606.ENSP00000364119.

PTM databases

iPTMnetiP20042.
PhosphoSitePlusiP20042.
SwissPalmiP20042.

Polymorphism and mutation databases

BioMutaiEIF2S2.
DMDMi12644154.

Proteomic databases

EPDiP20042.
PaxDbiP20042.
PeptideAtlasiP20042.
PRIDEiP20042.
TopDownProteomicsiP20042.

Protocols and materials databases

DNASUi8894.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000374980; ENSP00000364119; ENSG00000125977.
GeneIDi8894.
KEGGihsa:8894.

Organism-specific databases

CTDi8894.
DisGeNETi8894.
GeneCardsiEIF2S2.
HGNCiHGNC:3266. EIF2S2.
HPAiCAB034415.
HPA041262.
HPA041332.
MIMi603908. gene.
neXtProtiNX_P20042.
OpenTargetsiENSG00000125977.
PharmGKBiPA27696.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2768. Eukaryota.
COG1601. LUCA.
GeneTreeiENSGT00390000001804.
HOGENOMiHOG000107198.
HOVERGENiHBG000927.
InParanoidiP20042.
KOiK03238.
OMAiLSFKQMI.
OrthoDBiEOG091G0H6A.
PhylomeDBiP20042.
TreeFamiTF101503.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000125977-MONOMER.
ReactomeiR-HSA-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-HSA-382556. ABC-family proteins mediated transport.
R-HSA-72649. Translation initiation complex formation.
R-HSA-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-HSA-72702. Ribosomal scanning and start codon recognition.
R-HSA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-HSA-72731. Recycling of eIF2:GDP.
SIGNORiP20042.

Miscellaneous databases

ChiTaRSiEIF2S2. human.
GeneWikiiEIF2S2.
GenomeRNAii8894.
PROiP20042.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000125977.
CleanExiHS_EIF2S2.
ExpressionAtlasiP20042. baseline and differential.
GenevisibleiP20042. HS.

Family and domain databases

Gene3Di3.30.30.50. 1 hit.
InterProiIPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
[Graphical view]
PfamiPF01873. eIF-5_eIF-2B. 1 hit.
[Graphical view]
SMARTiSM00653. eIF2B_5. 1 hit.
[Graphical view]
SUPFAMiSSF100966. SSF100966. 1 hit.
SSF75689. SSF75689. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIF2B_HUMAN
AccessioniPrimary (citable) accession number: P20042
Secondary accession number(s): Q9BVU0, Q9UJE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 177 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. Translation initiation factors
    List of translation initiation factor entries
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.