Reviewed, UniProtKB/Swiss-Prot P20041 (PGLR2_RALSO)

Names and origin

Protein names

Recommended name:
    Polygalacturonase
      Short name=PGA
    EC=3.2.1.15
Alternative name(s):
    Pectinase

Gene names

Name:

pglA

Organism

Ralstonia solanacearum (Pseudomonas solanacearum)

Taxonomic identifier

305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Contributes to the wilt disease production on tomato.
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subunit structure	Monomer.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.1
	Chain	22 – 529	508	Polygalacturonase		PRO_0000024761
Regions
	Region	516 – 529	14	Required for PGA export across the outer membrane and catalytic activity
Sites
	Active site	305	1	Proton donor By similarity
	Active site	331	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P20041-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: F7438D7C007E401B	FASTA	529	54,636
	10 20 30 40 50 60 MNHRYTLLAL AAAALSAGAH ATGTSVTAPW GEVAEPSLPA DSAVCKTLSA SITPIKGSVD 70 80 90 100 110 120 SVDGNPANSQ PDASRIQSAI DNCPAGQAVK LVKGSAGESG FLSGSLKLKS GVTLWIDTGV 130 140 150 160 170 180 TLFASRNPAD YDNGLGTCGT ATTSNDKSCN ALIVARDTAG SGIVGAGAID GRGGSLVTSG 190 200 210 220 230 240 PNANRLTWWD IAYLNKTKGL NQQNPRLIQT YNGSAFTLYG VTVQNSPNFH IVTTGTSGVT 250 260 270 280 290 300 AWGIKIVTPS LAYAVAGYKC PSGSTPDKVT PATCFTPETV KNTDGFDPGQ STNVVLAYSY 310 320 330 340 350 360 INTGDDHVAV KASSGPTRNL LFAHNHFYYG HGLSIGSETN TGVSNMLVTD LTMDGNDSSA 370 380 390 400 410 420 GNGLRIKSDA SRGGKVTNIV YDGICMRNVK EPLVFDPFYS SVKGSLYPNF TNIVVKNFHD 430 440 450 460 470 480 LGSAKSIKRT MTFLGYKANK QKNPLTITLD NVVFDGTLPA FEGSHYGGPA SPNGVHFTFG 490 500 510 520 GTGPVSFADA IVTSSTTDVT VTGTPGTAAA VDCSKAFVPL KSVAPTSPI

References

[1]	"DNA sequence analysis of pglA and mechanism of export of its polygalacturonase product from Pseudomonas solanacearum." Huang J., Schell M.A. J. Bacteriol. 172:3879-3887(1990) [PubMed: 2193922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 22-39. Strain: AW.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M33692 Genomic DNA. Translation: AAA25931.1.
PIR	A44508.
3D structure databases
HSSP	HSSP built from PDB template 1BHE based on UniProtKB P26509.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 97066.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR2_RALSO

Accession

Primary (citable) accession number: P20041

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)