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Protein

Platelet-derived growth factor subunit A

Gene

Pdgfa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB.7 Publications

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • angiogenesis Source: MGI
  • animal organ morphogenesis Source: MGI
  • blood coagulation Source: GO_Central
  • bone development Source: MGI
  • cell projection assembly Source: MGI
  • digestive tract development Source: UniProtKB
  • embryonic lung development Source: BHF-UCL
  • hair follicle development Source: MGI
  • lung alveolus development Source: UniProtKB
  • negative chemotaxis Source: UniProtKB
  • negative regulation of phosphatidylinositol biosynthetic process Source: UniProtKB
  • negative regulation of platelet activation Source: UniProtKB
  • platelet-derived growth factor receptor signaling pathway Source: UniProtKB
  • positive regulation of cell division Source: UniProtKB-KW
  • positive regulation of cell migration Source: UniProtKB
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of DNA replication Source: UniProtKB
  • positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • positive regulation of fibroblast proliferation Source: UniProtKB
  • positive regulation of MAPK cascade Source: UniProtKB
  • positive regulation of MAP kinase activity Source: UniProtKB
  • positive regulation of mesenchymal cell proliferation Source: MGI
  • positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway Source: UniProtKB
  • positive regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
  • positive regulation of protein autophosphorylation Source: UniProtKB
  • positive regulation of protein kinase B signaling Source: UniProtKB
  • regulation of branching involved in salivary gland morphogenesis by epithelial-mesenchymal signaling Source: MGI
  • regulation of peptidyl-tyrosine phosphorylation Source: MGI
  • regulation of smooth muscle cell migration Source: UniProtKB
  • response to wounding Source: UniProtKB
  • skin development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Growth factor, Mitogen

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-derived growth factor subunit A
Short name:
PDGF subunit A
Alternative name(s):
PDGF-1
Platelet-derived growth factor A chain
Platelet-derived growth factor alpha polypeptide
Gene namesi
Name:Pdgfa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97527. Pdgfa.

Subcellular locationi

  • Secreted

  • Note: Released by platelets upon wounding.

GO - Cellular componenti

  • cell surface Source: UniProtKB
  • extracellular space Source: BHF-UCL
  • membrane Source: InterPro
  • microvillus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Lethal, due to defects in cell proliferation and migration, leading to defects in the development of the embryonic lung, gastrointestinal tract, oligodendrocytes and Leydig cells.7 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Add BLAST20
PropeptideiPRO_000002335821 – 86Removed in mature formAdd BLAST66
ChainiPRO_000002335987 – 211Platelet-derived growth factor subunit AAdd BLAST125

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi96 ↔ 140By similarity
Disulfide bondi123InterchainBy similarity
Disulfide bondi129 ↔ 177By similarity
Disulfide bondi132InterchainBy similarity
Disulfide bondi133 ↔ 179By similarity
Glycosylationi134N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP20033.
PeptideAtlasiP20033.
PRIDEiP20033.

PTM databases

PhosphoSitePlusiP20033.

Expressioni

Tissue specificityi

Expression primarily localized in papillary regions with presumable expression in tubular cells comprising the loop of Henle. In the renal cortex, a widespread expression seen in the vascular smooth muscle cells and is barely detectable in interstitial cells.1 Publication

Gene expression databases

CleanExiMM_PDGFA.

Interactioni

Subunit structurei

Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFB; antiparallel disulfide-linked dimer. The PDGFA homodimer interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with CSPG4 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000075463.

Structurei

3D structure databases

ProteinModelPortaliP20033.
SMRiP20033.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni158 – 162Receptor binding siteSequence analysis5

Domaini

The long form contains a basic insert which acts as a cell retention signal.

Sequence similaritiesi

Belongs to the PDGF/VEGF growth factor family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IEXJ. Eukaryota.
ENOG4111GMH. LUCA.
HOGENOMiHOG000286027.
HOVERGENiHBG053546.
InParanoidiP20033.
KOiK04359.
PhylomeDBiP20033.

Family and domain databases

CDDicd00135. PDGF. 1 hit.
Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
IPR006782. PDGF_N.
[Graphical view]
PfamiPF00341. PDGF. 1 hit.
PF04692. PDGF_N. 1 hit.
[Graphical view]
SMARTiSM00141. PDGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P20033-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRTWACLLLL GCGYLAHALA EEAEIPRELI ERLARSQIHS IRDLQRLLEI
60 70 80 90 100
DSVGAEDALE TSLRAHGSHA INHVPEKRPV PIRRKRSIEE AVPAVCKTRT
110 120 130 140 150
VIYEIPRSQV DPTSANFLIW PPCVEVKRCT GCCNTSSVKC QPSRVHHRSV
160 170 180 190 200
KVAKVEYVRK KPKLKEVQVR LEEHLECACA TSNLNPDHRE EETGRRRESG
210
KNRKRKRLKP T
Length:211
Mass (Da):24,102
Last modified:October 1, 1996 - v2
Checksum:iAC4345A10ECF4B39
GO
Isoform Short (identifier: P20033-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     194-196: GRR → DVR
     197-211: Missing.

Show »
Length:196
Mass (Da):22,267
Checksum:i3F219DA70E4007D4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92V → I in AAA39903 (PubMed:2155144).Curated1
Sequence conflicti174H → D in AAB28740 (PubMed:1340209).Curated1
Sequence conflicti174H → D in AAB28741 (PubMed:1340209).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004604194 – 196GRR → DVR in isoform Short. 2 Publications3
Alternative sequenceiVSP_004605197 – 211Missing in isoform Short. 2 PublicationsAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S66873
, S66868, S66869, S66870, S66871, S66872 Genomic DNA. Translation: AAB28740.2.
S66874
, S66868, S66869, S66870, S66871, S66872 Genomic DNA. Translation: AAB28741.2.
M29464 mRNA. Translation: AAA39903.1.
CCDSiCCDS19802.1. [P20033-2]
PIRiA37359.
RefSeqiNP_032834.1. NM_008808.3.
UniGeneiMm.2675.

Genome annotation databases

GeneIDi18590.
KEGGimmu:18590.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S66873
, S66868, S66869, S66870, S66871, S66872 Genomic DNA. Translation: AAB28740.2.
S66874
, S66868, S66869, S66870, S66871, S66872 Genomic DNA. Translation: AAB28741.2.
M29464 mRNA. Translation: AAA39903.1.
CCDSiCCDS19802.1. [P20033-2]
PIRiA37359.
RefSeqiNP_032834.1. NM_008808.3.
UniGeneiMm.2675.

3D structure databases

ProteinModelPortaliP20033.
SMRiP20033.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000075463.

PTM databases

PhosphoSitePlusiP20033.

Proteomic databases

PaxDbiP20033.
PeptideAtlasiP20033.
PRIDEiP20033.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18590.
KEGGimmu:18590.

Organism-specific databases

CTDi5154.
MGIiMGI:97527. Pdgfa.

Phylogenomic databases

eggNOGiENOG410IEXJ. Eukaryota.
ENOG4111GMH. LUCA.
HOGENOMiHOG000286027.
HOVERGENiHBG053546.
InParanoidiP20033.
KOiK04359.
PhylomeDBiP20033.

Miscellaneous databases

PROiP20033.
SOURCEiSearch...

Gene expression databases

CleanExiMM_PDGFA.

Family and domain databases

CDDicd00135. PDGF. 1 hit.
Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
IPR006782. PDGF_N.
[Graphical view]
PfamiPF00341. PDGF. 1 hit.
PF04692. PDGF_N. 1 hit.
[Graphical view]
SMARTiSM00141. PDGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDGFA_MOUSE
AccessioniPrimary (citable) accession number: P20033
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.