Reviewed, UniProtKB/Swiss-Prot P20028 (RPA2_DROME)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase I subunit RPA2
      Short name=RNA polymerase I subunit 2
    EC=2.7.7.6
Alternative name(s):
    RPA135

Gene names

Name:	RpI135
ORF Names:	CG4033

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1129 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits By similarity.
Subcellular location	Nucleus › nucleolus By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1129	1129	DNA-directed RNA polymerase I subunit RPA2		PRO_0000048075
Regions
	Zinc finger	1061 – 1093	33	C4-type Potential
Experimental info
	Sequence conflict	369	1	A → S in CAA35185. Ref.1
	Sequence conflict	617	1	M → L in CAA35185. Ref.1
	Sequence conflict	727	1	N → I in CAA35185. Ref.1
	Sequence conflict	855	1	K → T in CAA35185. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P20028-1 [UniParc]. Last modified October 25, 2005. Version 2. Checksum: DB39B863EF48487B	FASTA	1,129	128,444
	10 20 30 40 50 60 MLEEMQQMKT IPVLTNSRPE FKQIPKKLSR HLANLGGPHV DSFDEMLTVG LDNSAKHMIP 70 80 90 100 110 120 NHWLSPAGEK ISMKVESIWI AKPKVPQDVI DVRTREIYPT DSRQLHVSYS GMCSVRLGWS 130 140 150 160 170 180 VNGVQKTPIN MDLGEVPIML RSKACNLGQA TPEEMVKHGE HDSEWGGIFV IRGNEKIVRM 190 200 210 220 230 240 LIMTRRNHPI CVKRSSWKDR GQNFSDLGML VQTVREDESS LSNVVHYLNN GTAKFMFSHV 250 260 270 280 290 300 KRLSYVPVCL ILKCLMDYTD EEIYNRLVQG YESDQYYVSC VQAMLREVQN ENVYTHAQCK 310 320 330 340 350 360 SFIGNLFRAR FPEVPEWQPD DDVTDFILRE RVMIHLDTYE DKFQLIVFMI QKLFQCAQGK 370 380 390 400 410 420 YKVENVDSAM MQEVLLPGHL YQKYLSERVE SWVSQVRRCL QKKLTSPDAL VTSAVMTQCM 430 440 450 460 470 480 RQAGGVGRAI ESFLATGNIA SRTGLGLMQN SGLVIMAENI NRMRYMSHFR AIHRGSYFTT 490 500 510 520 530 540 MRTTEARQLL PDAWGFICPV HTPDGTPCGL LNHLTLTCEI SMRPDPKLVK AIPKHLIDMG 550 560 570 580 590 600 MMPLSNRRYL GEKLYVVFLD GKHLGHIHQS EAEKIVDELR YGKIFGTLPQ MMEIGFIPFK 610 620 630 640 650 660 KNGQFPGLYI ATGPARMMRP VWNLKWKRVE YIGTLEQLYM EIAIDAKEMY PDFTTHLELA 670 680 690 700 710 720 KTHFMSNLAN LIPMPDYNQS PRNMYQCQMG KQTMGTPCLN WPKQAANKLY RLQTPGTPLF 730 740 750 760 770 780 RPVHYDNIQL DDFAMGTNAI VAVISYTGYD MEDAMIINKA AYERGFAYGS IYKTKFLTLD 790 800 810 820 830 840 KKSSYFARHP HMPELIKHLD TDGLPHPGSK LSYGSPLYCY FDGEVATYKV VKMDEKEDCI 850 860 870 880 890 900 VESIRQLGSF DLSPKKMVAI TLRVPRPATI GDKFASRAGQ KGICSQKYPA EDLPFTESGL 910 920 930 940 950 960 IPDIVFNPHG FPSRMTIAMM IETMAGKGAA IHGNVYDATP FRFSEENTAI DYFGKMLEAG 970 980 990 1000 1010 1020 GYNYYGTERL YSGVDGREMT ADIFFGVVHY QRLRHMVFDK WQVRSTGAVE ARTHQPIKGR 1030 1040 1050 1060 1070 1080 KRGGGVRFGE MERDALISHG AAFLLQDRLF HNSDKTHTLV CHKCGSILAP LQRIVKRNET 1090 1100 1110 1120 GGLSSQPDTC RLCGDNSSVS MIEIPFSFKY LVTELSSVNI NARFKLNEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure and functional aspects of the gene coding for the second-largest subunit of RNA polymerase III of Drosophila." Kontermann R., Sitzler S., Seifarth W., Petersen G., Bautz E.K.F. Mol. Gen. Genet. 219:373-380(1989) [PubMed: 2482932] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X17298 Genomic DNA. Translation: CAA35185.1. AE014134 Genomic DNA. Translation: AAF51503.1. AY075569 mRNA. Translation: AAL68376.1.
PIR	JQ0354.
RefSeq	NP_476708.1.
UniGene	Dm.30753
3D structure databases
HSSP	HSSP built from PDB template 1K83 based on UniProtKB P08518.
ModBase	Search...
Protein-protein interaction databases
STRING	P20028.
Genome annotation databases
Ensembl	FBtr0078054; FBpp0077714; FBgn0003278; Drosophila melanogaster. [Genome view]
GeneID	33210.
KEGG	dme:Dmel_CG4033.
NMPDR	fig|7227.3.peg.89.
Organism-specific databases
CTD	33210.
FlyBase	FBgn0003278. RpI135.
Phylogenomic databases
HOGENOM	P20028.
OMA	CGLMNHM.
Enzyme and pathway databases
BRENDA	2.7.7.6. 48.
Gene expression databases
ArrayExpress	P20028.
Bgee	P20028.
GermOnline	CG4033. Drosophila melanogaster.
Family and domain databases
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR009674. RNA_pol_Rpa2-specific. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007647. RNA_pol_Rpb2_5. IPR007641. RNA_pol_Rpb2_7. [Graphical view]
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF06883. RNA_pol_Rpa2_4. 1 hit. PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF04567. RNA_pol_Rpb2_5. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	782449.

Entry information

Entry name

RPA2_DROME

Accession

Primary (citable) accession number: P20028
Secondary accession number(s): Q9VPP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	October 25, 2005
Last modified:	November 3, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project