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Protein

Probable cadmium-transporting ATPase

Gene

cadA

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This electroneutral antiporter ejects one cadmium ion while accumulating two protons by an energy-dependent efflux mechanism.

Catalytic activityi

ATP + H2O + Cd2+(In) = ADP + phosphate + Cd2+(Out).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi23CadmiumPROSITE-ProRule annotation1
Metal bindingi26CadmiumPROSITE-ProRule annotation1
Active sitei4154-aspartylphosphate intermediateCurated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antiport, Cadmium resistance, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Cadmium, Magnesium, Metal-binding, Nucleotide-binding

Protein family/group databases

TCDBi3.A.3.6.1. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cadmium-transporting ATPase (EC:3.6.3.3)
Alternative name(s):
Cadmium efflux ATPase
Gene namesi
Name:cadA
Encoded oniPlasmid pI2580 Publication
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei106 – 126HelicalSequence analysisAdd BLAST21
Transmembranei130 – 150HelicalSequence analysisAdd BLAST21
Transmembranei336 – 356HelicalSequence analysisAdd BLAST21
Transmembranei364 – 384HelicalSequence analysisAdd BLAST21
Transmembranei672 – 694HelicalSequence analysisAdd BLAST23
Transmembranei699 – 721HelicalSequence analysisAdd BLAST23

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462481 – 727Probable cadmium-transporting ATPaseAdd BLAST727

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliP20021.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 92HMAPROSITE-ProRule annotationAdd BLAST80

Sequence similaritiesi

Contains 1 HMA domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

CDDicd00371. HMA. 1 hit.
Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
[Graphical view]
PRINTSiPR00941. CDATPASE.
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P20021-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEQKVKLME EEMNVYRVQG FTCANCAGKF EKNVKKIPGV QDAKVNFGAS
60 70 80 90 100
KIDVYGNASV EELEKAGAFE NLKVSPEKLA NQTIQRVKDD TKAHKEEKTP
110 120 130 140 150
FYKKHSTLLF ATLLIAFGYL SHFVNGEDNL VTSMLFVGSI VIGGYSLFKV
160 170 180 190 200
GFQNLIRFDF DMKTLMTVAV IGATIIGKWA EASIVVILFA ISEALERFSM
210 220 230 240 250
DRSRQSIRSL MDIAPKEALV RRNGQEIIIH VDDIAVGDIM IVKPGEKIAM
260 270 280 290 300
DGIIVNGLSA VNQAAITGES VPVSKAVDDE VFAGTLNEEG LIEVKITKYV
310 320 330 340 350
EDTTITKIIH LVEEAQGERA PAQAFVDKFA KYYTPIIMVI AALVAVVPPL
360 370 380 390 400
FFGGSWDTWV YQGLAVLVVG CPCALVISTP ISIVSAIGNA AKKGVLVKGG
410 420 430 440 450
VYLEKLGAIK TVAFDKTGTL TKGVPVVTDF EVLNDQVEEK ELFSIITALE
460 470 480 490 500
YRSQHPLASA IMKKAEQDNI PYSNVQVEEF TSITGRGIKG IVNGTTYYIG
510 520 530 540 550
SPKLFKELNV SDFSLGFENN VKILQNQGKT AMIIGTEKTI LGVIAVADEV
560 570 580 590 600
RETSKNVIQK LHQLGIKQTI MLTGDNQGTA NAIGTHVGVS DIQSELMPQD
610 620 630 640 650
KLDYIKKMQS EYDNVAMIGD GVNDAPALAA STVGIAMGGA GTDTAIETAD
660 670 680 690 700
IALMGDDLSK LPFAVRLSRK TLNIIKANIT FAIGIKIIAL LLVIPGWLTL
710 720
WIAILSDMGA TILVALNSLR LMRVKDK
Length:727
Mass (Da):78,812
Last modified:February 1, 1991 - v1
Checksum:i7281664ED658FBE9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04551 Genomic DNA. Translation: AAB59154.1.
PIRiA32561.
RefSeqiWP_000003267.1. NZ_JXZP01000002.1.
YP_006937600.1. NC_013319.1.
YP_006938635.1. NC_013347.1.
YP_006938768.1. NC_013352.1.

Genome annotation databases

GeneIDi13874748.
13875812.
13875979.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04551 Genomic DNA. Translation: AAB59154.1.
PIRiA32561.
RefSeqiWP_000003267.1. NZ_JXZP01000002.1.
YP_006937600.1. NC_013319.1.
YP_006938635.1. NC_013347.1.
YP_006938768.1. NC_013352.1.

3D structure databases

ProteinModelPortaliP20021.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi3.A.3.6.1. the p-type atpase (p-atpase) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13874748.
13875812.
13875979.

Family and domain databases

CDDicd00371. HMA. 1 hit.
Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
[Graphical view]
PRINTSiPR00941. CDATPASE.
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCADA1_STAAU
AccessioniPrimary (citable) accession number: P20021
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 30, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.