Probable cadmium-transporting ATPase

Preferred order of sections

Names and origin

Protein names

Recommended name:
Probable cadmium-transporting ATPase
EC=3.6.3.3

Alternative name(s):
Cadmium efflux ATPase

Gene names

Name:

cadA

Encoded on

Plasmid pI258

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This electroneutral antiporter ejects one cadmium ion while accumulating two protons by an energy-dependent efflux mechanism.
Catalytic activity	ATP + H₂O + Cd²⁺(In) = ADP + phosphate + Cd²⁺(Out).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. [View classification] Contains 1 HMA domain.

Ontologies

Keywords
Biological process	Antiport Cadmium resistance Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Cadmium Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Plasmid
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro response to cadmium ion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW antiporter activity Inferred from electronic annotation. Source: UniProtKB-KW cadmium-exporting ATPase activity Inferred from electronic annotation. Source: EC cation channel activity Inferred from electronic annotation. Source: InterPro copper-exporting ATPase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 727

727

Probable cadmium-transporting ATPase

PRO_0000046248

Regions

Transmembrane

106 – 126

21

Helical; Potential

Transmembrane

130 – 150

21

Helical; Potential

Transmembrane

336 – 356

21

Helical; Potential

Transmembrane

364 – 384

21

Helical; Potential

Transmembrane

672 – 694

23

Helical; Potential

Transmembrane

699 – 721

23

Helical; Potential

Domain

13 – 92

80

HMA

Sites

Active site

415

1

4-aspartylphosphate intermediate Probable

Metal binding

23

1

Cadmium Potential

Metal binding

26

1

Cadmium Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P20021 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 7281664ED658FBE9
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FASTA

727

78,812

        10         20         30         40         50         60 
MSEQKVKLME EEMNVYRVQG FTCANCAGKF EKNVKKIPGV QDAKVNFGAS KIDVYGNASV 

        70         80         90        100        110        120 
EELEKAGAFE NLKVSPEKLA NQTIQRVKDD TKAHKEEKTP FYKKHSTLLF ATLLIAFGYL 

       130        140        150        160        170        180 
SHFVNGEDNL VTSMLFVGSI VIGGYSLFKV GFQNLIRFDF DMKTLMTVAV IGATIIGKWA 

       190        200        210        220        230        240 
EASIVVILFA ISEALERFSM DRSRQSIRSL MDIAPKEALV RRNGQEIIIH VDDIAVGDIM 

       250        260        270        280        290        300 
IVKPGEKIAM DGIIVNGLSA VNQAAITGES VPVSKAVDDE VFAGTLNEEG LIEVKITKYV 

       310        320        330        340        350        360 
EDTTITKIIH LVEEAQGERA PAQAFVDKFA KYYTPIIMVI AALVAVVPPL FFGGSWDTWV 

       370        380        390        400        410        420 
YQGLAVLVVG CPCALVISTP ISIVSAIGNA AKKGVLVKGG VYLEKLGAIK TVAFDKTGTL 

       430        440        450        460        470        480 
TKGVPVVTDF EVLNDQVEEK ELFSIITALE YRSQHPLASA IMKKAEQDNI PYSNVQVEEF 

       490        500        510        520        530        540 
TSITGRGIKG IVNGTTYYIG SPKLFKELNV SDFSLGFENN VKILQNQGKT AMIIGTEKTI 

       550        560        570        580        590        600 
LGVIAVADEV RETSKNVIQK LHQLGIKQTI MLTGDNQGTA NAIGTHVGVS DIQSELMPQD 

       610        620        630        640        650        660 
KLDYIKKMQS EYDNVAMIGD GVNDAPALAA STVGIAMGGA GTDTAIETAD IALMGDDLSK 

       670        680        690        700        710        720 
LPFAVRLSRK TLNIIKANIT FAIGIKIIAL LLVIPGWLTL WIAILSDMGA TILVALNSLR 


LMRVKDK

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References

[1]	"Cadmium resistance from Staphylococcus aureus plasmid pI258 cadA gene results from a cadmium-efflux ATPase." Nucifora G., Chu L., Misra T.K., Silver S. Proc. Natl. Acad. Sci. U.S.A. 86:3544-3548(1989) [PubMed: 2524829] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BD224.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04551 Genomic DNA. Translation: AAB59154.1.
PIR	A32561.
3D structure databases
ProteinModelPortal	P20021.
SMR	P20021. Positions 12-80.
ModBase	Search...
Protein family/group databases
TCDB	3.A.3.6.1. P-type ATPase (P-ATPase) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR006403. ATPase_P-typ_cat/Cu-transptr. IPR001366. ATPase_P-typ_Cd-transp. IPR006404. ATPase_P-typ_Cd/Co/Hg/Pb/Zn. IPR023300. ATPase_P-typ_cyto_domA. IPR023299. ATPase_P-typ_cyto_domN. IPR006416. ATPase_P-typ_heavy-metal. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. IPR023214. HAD-like_dom. IPR017969. Heavy-metal-associated_CS. IPR006121. HeavyMe-assoc_HMA. [Graphical view]
Gene3D	G3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 1 hit. G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit. G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF00403. HMA. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00941. CDATPASE.
SUPFAM	SSF56784. HAD-like_dom. 1 hit. SSF55008. HeavyMe_transpt. 1 hit.
TIGRFAMs	TIGR01511. ATPase-IB1_Cu. 1 hit. TIGR01512. ATPase-IB2_Cd. 1 hit. TIGR01525. ATPase-IB_hvy. 1 hit. TIGR01494. ATPase_P-type. 1 hit.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. PS01047. HMA_1. 1 hit. PS50846. HMA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CADA1_STAAU

Accession

Primary (citable) accession number: P20021

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	September 21, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Family and domain databases

Entry information

Relevant documents